Abstract:
:A quantitative method was developed for the assay of vitamin K in human colostrum and milk. The procedure combines preparative and analytical chromatography on silica gel in a nitrogen atmosphere followed by reversed phase high performance liquid chromatography (HPLC). Two HPLC steps were used: gradient separation with ultraviolet (UV) detection followed by isocratic separation detected electrochemically. Due to co-migrating impurities, UV detection alone is insufficient for identification of vitamin K. Exogenous vitamin K was shown to equilibrate with endogenous vitamin K in the samples. A statistical method was incorporated to control for experimental variability. Vitamin K1 was analyzed in 16 pooled milk samples from 7 donors and in individual samples from 15 donors at 1 month post-partum. Vitamin K1 was present at 2.94 +/- 1.94 and 3.15 +/- 2.87 ng/mL in pools and in individuals, respectively. Menaquinones, the bacterial form of the vitamin, were not detected. The significance of experimental variation to studies of vitamin K in individuals is discussed.
journal_name
Lipidsjournal_title
Lipidsauthors
Canfield LM,Hopkinson JM,Lima AF,Martin GS,Sugimoto K,Burr J,Clark L,McGee DLdoi
10.1007/BF02537985subject
Has Abstractpub_date
1990-07-01 00:00:00pages
406-11issue
7eissn
0024-4201issn
1558-9307journal_volume
25pub_type
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