Quantitation of vitamin K in human milk.


:A quantitative method was developed for the assay of vitamin K in human colostrum and milk. The procedure combines preparative and analytical chromatography on silica gel in a nitrogen atmosphere followed by reversed phase high performance liquid chromatography (HPLC). Two HPLC steps were used: gradient separation with ultraviolet (UV) detection followed by isocratic separation detected electrochemically. Due to co-migrating impurities, UV detection alone is insufficient for identification of vitamin K. Exogenous vitamin K was shown to equilibrate with endogenous vitamin K in the samples. A statistical method was incorporated to control for experimental variability. Vitamin K1 was analyzed in 16 pooled milk samples from 7 donors and in individual samples from 15 donors at 1 month post-partum. Vitamin K1 was present at 2.94 +/- 1.94 and 3.15 +/- 2.87 ng/mL in pools and in individuals, respectively. Menaquinones, the bacterial form of the vitamin, were not detected. The significance of experimental variation to studies of vitamin K in individuals is discussed.






Canfield LM,Hopkinson JM,Lima AF,Martin GS,Sugimoto K,Burr J,Clark L,McGee DL




Has Abstract


1990-07-01 00:00:00














  • Dietary CLA and DHA modify skin properties in mice.

    abstract::This study investigated the influence of PUFA on the properties of mouse skin. Mice (3 wk old) were given free access to oils high in linoleic acid, CLA, or DHA for 4 wk. At the end of the experiment, their skins were compared by both biochemical and histological methods. No significant differences in lipid and collag...


    pub_type: 杂志文章


    authors: Oikawa D,Nakanishi T,Nakamura Y,Takahashi Y,Yamamoto T,Shiba N,Tobisa M,Takagi T,Iwamoto H,Tachibana T,Furuse M

    更新日期:2003-06-01 00:00:00

  • Composition of lipids of bovine optic nerve.

    abstract::Lipids from bovine optic nerve were analyzed. The total content of 16.5% by weight included 27.2% nonpolar lipids, 26.1% glycolipids, and 46.7% phospholipids by weight. Free cholesterol was the major component of the nonpolar lipid fraction. The cerebrosides, 73.5% of total glycolipids, were separated by thin layer ch...


    pub_type: 杂志文章


    authors: Das SK,Steen ME,McCullough MS,Bhattacharyya DK

    更新日期:1978-10-01 00:00:00

  • Lecithin-cholesterol acyltransferase: inhibition by local anesthetics in plasma from man and experimental animals in vitro.

    abstract::Lecithin-cholesterol acyltransferase (LCAT, EC was assayed in vitro in plasma from normal man, rat, rabbit and dog by following the formation of 14C-cholesteryl esters subsequent to labeling the plasma with 14C-cholesterol in vitro. In all species examined, various local anesthetics were found to inhibit LCA...


    pub_type: 杂志文章


    authors: Bell FP,Hubert EV

    更新日期:1980-10-01 00:00:00

  • Membrane docosahexaenoic acid vs. eicosapentaenoic acid and the beating function of the cardiomyocyte and its regulation through the adrenergic receptors.

    abstract::The beta-adrenergic system in cardiac muscle cells is influenced by the n-3 polyunsaturated fatty acid (PUFA) content in cell membranes. This study deals with the specific effect of docosahexaenoic acid (DHA) as compared to other n-3 PUFA, without modification of the arachidonic acid content. Increasing the DHA conten...


    pub_type: 杂志文章


    authors: Grynberg A,Fournier A,Sergiel JP,Athias P

    更新日期:1996-03-01 00:00:00

  • Factors affecting the storage and excretion of toxic lipophilic xenobiotics.

    abstract::Lipophilic toxins have been introduced into the environment both as functional compounds, such as pesticides, and as industrial waste from incineration or the manufacture of electrical transformer components. Among these substances are compounds that are carcinogenic and that affect the endocrine system. Accidental hi...


    pub_type: 杂志文章,评审


    authors: Jandacek RJ,Tso P

    更新日期:2001-12-01 00:00:00

  • Dietary sunflower oil reduces plasma and liver triacylglycerols in fasting rats and is associated with decreased liver microsomal phosphatidate phosphohydrolase activity.

    abstract::Plasma and liver lipids were studied in male weanling rats fed diets containing moderate levels of fat (6% by weight) as sunflower oil (SF diet, rich in linoleic acid), salmon oil (SM diet, rich in long-chain n-3 fatty acids), or a blend of peanut and rapeseed oil (PR diet, rich in oleic acid). After nine weeks of fee...


    pub_type: 杂志文章


    authors: Frémont L,Gozzelino MT

    更新日期:1996-08-01 00:00:00

  • Dual action of neutral sphingomyelinase on rat hepatocytes: activation of cholesteryl ester metabolism and biliary cholesterol secretion and inhibition of VLDL secretion.

    abstract::To address the role of cell membrane neutral sphingomyelinase (EC; SMase) in the regulation of cholesterol metabolism in the liver parenchymal cell, we examined the effect of exogenous neutral SMase on the metabolism of cholesteryl esters and the secretion of VLDL and biliary lipids in isolated rat hepatocyte...


    pub_type: 杂志文章


    authors: Liza M,Chico Y,Fresnedo O,Ochoa B

    更新日期:2003-01-01 00:00:00

  • Inhibition of 12- and 15-lipoxygenase activities and protection of human and tilapia low density lipoprotein oxidation by I-Tiao-Gung (Glycine tomentella).

    abstract::I-Tiao-Gung, Glycine tomentella, has been used extensively as a traditional herbal medicine to relieve physical pain, but its bioactivity has not been studied systematically. Ninety-five percent ethanol extracts of G. tomentella (GT-E) showed antioxidant activity in human plasma by prolonging the lag phase (+Tlag) of ...


    pub_type: 杂志文章


    authors: Chen TY,Shiao MS,Pan BS

    更新日期:2005-11-01 00:00:00

  • Analytical Method for Diacylglycerol Kinase ζ Activity in Cells Using Protein Myristoylation and Liquid Chromatography-Tandem Mass Spectrometry.

    abstract::Specific inhibitors of diacylglycerol kinase (DGK) ζ can be promising anticancer medications via the activation of cancer immunity. Although the detection of cellular activities of target enzymes is essential for drug screening in addition to in vitro assays, it is difficult to detect the activity of DGKζ in cells. In...


    pub_type: 杂志文章


    authors: Honda S,Murakami C,Yamada H,Murakami Y,Ishizaki A,Sakane F

    更新日期:2019-11-01 00:00:00

  • Effects of dehydroepiandrosterone (DHEA) on hepatic lipid metabolism parameters and lipogenic gene mRNA expression in broiler chickens.

    abstract::The aim of the present study was to identify the effects of dehydroepiandrosterone (DHEA) on hepatic lipid metabolism parameters and lipogenic gene mRNA expression in broiler chickens. A total of 72 1-day-old broiler chicks received a common basal diet with DHEA added at either 0 (control), 5 or 20 mg/kg feed. In the ...


    pub_type: 杂志文章


    authors: Tang X,Ma H,Zou S,Chen W

    更新日期:2007-11-01 00:00:00

  • Enrichment of LDL with EPA and DHA decreased oxidized LDL-induced apoptosis in U937 cells.

    abstract::Oxidized LDL (oxLDL) may contribute to the accumulation of apoptotic cells in atherosclerotic plaques. Although it is well established in monophasic chemical systems that the highly unsaturated EPA and DHA will oxidize more readily than FA that contain fewer double bonds, our previous studies showed that enrichment of...


    pub_type: 杂志文章


    authors: Wu T,Geigerman C,Lee YS,Wander RC

    更新日期:2002-08-01 00:00:00

  • Fish consumption and blood lipids in three ethnic groups of Québec (Canada).

    abstract::The purpose of this study was to compare fish intake and plasma phospholipid concentrations of n-3 fatty acids, in particular of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), among representative population samples of Québecers, James Bay Cree, and Inuit of Nunavik (Canada). The relationships between the...


    pub_type: 杂志文章


    authors: Dewailly E,Blanchet C,Gingras S,Lemieux S,Holub BJ

    更新日期:2003-04-01 00:00:00

  • Beta(3)-adrenergic signaling acutely down regulates adipose triglyceride lipase in brown adipocytes.

    abstract::Mice exposed to cold rely upon brown adipose tissue (BAT)-mediated nonshivering thermogenesis to generate body heat using dietary glucose and lipids from the liver and white adipose tissue. In this report, we investigate how cold exposure affects the PI3 K/Akt signaling cascade and the expression of genes involved in ...


    pub_type: 杂志文章


    authors: Deiuliis JA,Liu LF,Belury MA,Rim JS,Shin S,Lee K

    更新日期:2010-06-01 00:00:00

  • The effect of short-term lipid infusion on liver function and biliary secretion in rats.

    abstract::This study was undertaken to determine the effect of various lipid emulsions on the hepato-biliary system in rats. Rats were randomly divided into six groups and infused continuously for 48 hr with either long-chain triglycerides (LCT), medium-chain triglycerides (MCT) or a mixture of MCT and LCT. One group infused wi...


    pub_type: 杂志文章


    authors: Rubin M,Halpern Z,Livoff A,Wennberg A,Tietz A,Antebi E,Lichtenberg D

    更新日期:1992-05-01 00:00:00

  • Headspace gas chromatography of volatile lipid peroxidation products from human red blood cell membranes.

    abstract::An improved headspace capillary gas chromatographic (GC) method was developed to measure the oxidative susceptibility of human red blood cell (RBC) membranes. This method analyzed volatile peroxidation products of both n-6 (hexanal and pentane) and n-3 (propanal) polyunsaturated fatty acids. Oxidative susceptibility t...


    pub_type: 杂志文章


    authors: Frankel EN,Tappel AL

    更新日期:1991-06-01 00:00:00

  • Recent advances in the purification, characterization and structure determination of lipases.

    abstract::Recently, lipases have been purified from mammalian, bacterial, fungal and plant sources by different methodologies. Purified lipases subsequently have been characterized for molecular size, metal binding capabilities, glycoside and phosphorus contents, and substrate specificities. Primary structures of several lipase...


    pub_type: 杂志文章,评审


    authors: Antonian E

    更新日期:1988-12-01 00:00:00

  • Kinetics of 14C distribution after tracer dose of 14C-lutein in an adult woman.

    abstract::Lutein is an oxygenated carotenoid (xanthophyll) found in dark green leafy vegetables. High intakes of lutein may lower the risk of age-related macular degeneration. Current understanding of human lutein metabolism as it might occur in vivo is incomplete. Therefore, we conducted a feasibility study where we dosed a no...


    pub_type: 杂志文章


    authors: de Moura FF,Ho CC,Getachew G,Hickenbottom S,Clifford AJ

    更新日期:2005-10-01 00:00:00

  • Long-chain Acyl-CoA levels in liver from rats fed high-fat diets: is it of significance for an increased peroxisomal beta-oxidation?

    abstract::The levels of long-chain acyl-CoA in the livers of rats given diets containing various amounts of dietary oils were investigated. Increasing the amount of soybean oil in the diet from 5% to 25% (w/w) led to a 40% increase in long-chain acyl-CoA. With partially hydrogenated marine oil, a sigmoidal dose-response curve w...


    pub_type: 杂志文章


    authors: Nilsson A,Thomassen MS,Christiansen E

    更新日期:1984-03-01 00:00:00

  • Transient activation of 1-O-alkyl-sn-glycero-3-phosphocholine: acetyl-CoA acetyltransferase during the incubation of macrophages.

    abstract::The activity of the platelet-activating factor (PAF)-synthesizing enzyme, 1-O-alkyl-sn-glycero-3-phosphocholine (lysoPAF):acetyl-CoA acetyltransferase (EC in alveolar macrophage lysate was found to be elevated after warming the cells to 37 degrees C. Such an increase in enzyme activity was detectable only wh...


    pub_type: 杂志文章


    authors: Sugiura T,Fukuda T,Cheng NN,Waku K

    更新日期:1991-11-01 00:00:00

  • Lipid content of swine influenza and other vaccines.

    abstract::An analysis of the lipids in swine influenza vaccines was performed, comparing six different lots of swine influenza, other influenza and noninfluenza vaccines. Cholesterol content and phospholipid content varied greatly, but there were no major differences between the types of vaccines. Appreciable amounts of phospha...


    pub_type: 杂志文章


    authors: Leskawa KC,Hogan EL,Dasgupta S,Chien JL,Erwin RE,Brostoff SW

    更新日期:1986-05-01 00:00:00

  • Trifluoperazine increases fatty acid turnover in phospholipids in cultured human fibroblasts.

    abstract::A 24-hr pretreatment of cultured human fibroblasts with trifluoperazine induced a marked increase in incorporation of saturated (stearic, palmitic) and unsaturated (oleic, arachidonic) fatty acids into phospholipids (1.5- to 2-fold for 5.10(-5) M trifluoperazine). Concomitantly, incorporation into cholesteryl esters w...


    pub_type: 杂志文章


    authors: Mazière C,Mazière JC,Mora L,Auclair M,Polonovski J

    更新日期:1988-05-01 00:00:00

  • Effects of ethionine and phenobarbital on the phosphatidylcholines of rat liver.

    abstract::The diglyceride species of phosphatidylcholines from livers of male rats after treatment of the animals with ethionine (1 mg/g divided among 4 doses 2 hr apart), phenobarbital (80 mg/kg each day for 3 days), or a combination of the two drugs were determined using gas chromatography. Ethionine treatment greatly elevate...


    pub_type: 杂志文章


    authors: Dyer RA,Klopfenstein WE

    更新日期:1977-11-01 00:00:00

  • Lysophosphatidylcholine acyltransferase activity during experimental cholelithiasis.

    abstract::The accumulation of (1-palmitoyl)lysophosphatidylcholine, lysolecithin, in gallbladder bile was observed during the first week of cholesterol-induced experimental cholelithiasis using the prairie dog model for cholesterol gallstone formation. Gallbladder fluid transport function decreased as bile lysolecithin concentr...


    pub_type: 杂志文章


    authors: Rutledge FA 2nd,Hickman DM,Dunn JJ,Frey CF,Matson RS

    更新日期:1981-10-01 00:00:00

  • Synthesis of phosphatidylcholine and phosphatidylethanolamine at different ages in the rat brain in vitro.

    abstract::The de novo synthesis of choline and ethanolamine phosphoglycerides in brain microsomes from 18 month-old male rats was investigated in vitro by using labeled cytidine-5'-diphosphate choline and cytidine-5'-diphosphate ethanolamine as lipid precursors. The rate of synthesis of the two phospholipid classes was found to...


    pub_type: 杂志文章


    authors: Brunetti M,Gaiti A,Porcellati G

    更新日期:1979-11-01 00:00:00

  • Highly unsaturated fatty acid synthesis in marine fish: cloning, functional characterization, and nutritional regulation of fatty acyl delta 6 desaturase of Atlantic cod (Gadus morhua L.).

    abstract::This study reports the cloning, functional characterization, tissue expression, and nutritional regulation of a delta6 fatty acyl desaturase of Atlantic cod (Gadus morhua). PCR primers were designed based on the sequences of conserved motifs in available fish desaturases and used to isolate a cDNA fragment from cod li...


    pub_type: 杂志文章


    authors: Tocher DR,Zheng X,Schlechtriem C,Hastings N,Dick JR,Teale AJ

    更新日期:2006-11-01 00:00:00

  • Triester glycerothiophosphates of cholecalciferol (vitamin D3).

    abstract::Tris(N,N-dimethyl)amide of phosphorous acid activated by addition of iodine at an optimal molar ratio of 1.05:0.05 was used as a phosphorylating reagent to synthesize cholecalciferyl-3-O-(1-2-isopropylidene-rac-glycero-3-O-)-thiophos phate and cholecalciferyl-3-O-(1,2-dipalmitoyl-rac-glycero-3-O-)thiophosphat e of met...


    pub_type: 杂志文章


    authors: Stamatov SD,Gronowitz S

    更新日期:1992-06-01 00:00:00

  • Polyunsaturated fatty acids of serum lipids in myocardial infarction.

    abstract::Two groups of volunteers had blood drawn for serum analysis of fatty acids. The first group was comprised of patients admitted to the hospital with possible myocardial infarction (MI). Blood was drawn at admission and at 12, 24 and 48 hr. These patients were subsequently divided into three groups, those with MI, those...


    pub_type: 杂志文章


    authors: Crofts JW,Ogburn PL Jr,Johnson SB,Holman RT

    更新日期:1988-06-01 00:00:00

  • Effect of synthetic phospholipids on platelet aggregation and serotonin release.

    abstract::1-O-Hexadecyl-2-O-acetyl-sn-glycero-3-phosphocholine (platelet-activating factor, PAF) is known to stimulate platelet aggregation and serotonin release in concentrations ranging from 10(-10)-10(-5) M. Since a variety of synthetic PAF analogues are potent antineoplastic agents in vitro and in vivo, it was the aim of th...


    pub_type: 杂志文章


    authors: Söling U,Eibl H,Nagel GA,Unger C

    更新日期:1987-11-01 00:00:00

  • Suppression of cholesterogenesis by plant constituents: review of Wisconsin contributions to NC-167.

    abstract::In animals, non-sterol metabolites of the mevalonate pathway act independently from receptor-mediated cholesterol uptake in the multivalent feedback regulation of mevalonate biosynthesis. Studies leading to the isolation and characterization of plant-borne suppressors of mevalonate biosynthesis are reviewed. We propos...


    pub_type: 杂志文章


    authors: Qureshi AA,Burger WC,Peterson DM,Elson C

    更新日期:1985-11-01 00:00:00

  • Photo-initiated peroxidation of lipids in micelles by azaaromatics.

    abstract::The monoazaaromatics, pyridine (1), hexyl nicotinate (2), and quinoline (3) and diazaaromatics, pyrimidine (4) and purine (5), readily act as photo-initiators for the peroxidation of methyl linoleate in 0.50 M SDS at 37 degrees C giving free radical chain oxidations of linoleate. Quantitative kinetic runs on the order...


    pub_type: 杂志文章


    authors: Barclay LR,Crowe E,Edwards CD

    更新日期:1997-03-01 00:00:00