Abstract:
:Ceramides are a family of signalling lipids with diverse physiological functions that include pro-differentiative and pro-apoptotic signalling. Ceramides and their derivatives are major constituents of myelin, maintaining neuronal conductivity. Ceramides are synthesized by ceramide synthases, of which there are six isoforms in mammals (CERS1-6). These enzymes catalyse the transfer of a variable length fatty acid to a sphingoid base, typically sphingosine or dihydrosphingosine. We previously reported a fluorescent thin-layer chromatography assay for ceramide synthase activity. In this paper we describe an improved fluorescent assay, using HPLC to achieve clear resolution of closely related ceramide species and to facilitate easy quantification of both product and substrate. Our HPLC assay protocol eliminates the need for a chloroform extraction step. Instead a simple three-step procedure is used: (1) reactions are run; (2) reactions are terminated with addition of methanol and centrifuged; (3) products are quantified with HPLC. HPLC resolution enables assays in which multiple fatty acid substrates are used in the same reaction. Using this approach, we show that CERS2 demonstrates a preference for the monounsaturated C24:1 fatty acid substrate compared to the saturated C24:0 substrate, potentially explaining why myelin is enriched in ceramides containing the monounsaturated form of very long chain fatty acids.
journal_name
Lipidsjournal_title
Lipidsauthors
Couttas TA,Lim XY,Don ASdoi
10.1007/s11745-014-3969-5subject
Has Abstractpub_date
2015-01-01 00:00:00pages
101-9issue
1eissn
0024-4201issn
1558-9307journal_volume
50pub_type
杂志文章相关文献
LIPIDS文献大全abstract::Minimal deviation hepatoma 7288 C cells were cultured in Swim's medium containing 10% serum for 48 hr. The growth medium was replaced with serum free media containing different concentrations of [1-14C] eicosa-8,11,14-trienoic acid and the cells were incubated for 24 hr. Incorporation into cell lipids, oxidation to CO...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02532312
更新日期:1975-12-01 00:00:00
abstract::Intact washed human platelets aggregated in response to paf-acether (paf) and did not metabolize [3H]paf at concentrations up to 10 nM. However, when platelets were lysed by exposure to pH 9.5, resulting in 37.5 +/- 2.5% (mean +/- SD, n = 3) lactic dehydrogenase (LDH) release, 20.5 +/- 5.7% of the radioactivity was de...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02536639
更新日期:1993-03-01 00:00:00
abstract::Plasma fatty acid (FA) composition is known to be an indicator of dietary fat quality, but the associations of other dietary factors with plasma FA composition remain unknown in children. We investigated the cross-sectional associations of food consumption with the proportions of FA and estimated desaturase activities...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-014-3894-7
更新日期:2014-05-01 00:00:00
abstract::Mycobacterium fortuitum utilizes certain stereoisometric mixtures of individual multimethyl branched alkanes as sole carbon source, including 2,6(R), 10(S), 14(RS)-tetramethylhexadecane; 2.6(R), 10(S), 14(RS)-tetramethylheptadecane; 2,6(RS), 10(RS)-trimethyltetradecane, and 2,6(R), 10(S)-trimethylpentadecane. Products...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02532587
更新日期:1976-01-01 00:00:00
abstract::Oxidized lipoprotein(a) (oxLp(a)) is a more potent marker of atherogenesis than native Lp(a). However, the molecular mechanisms of oxLp(a) activity are not clear. Reactive oxygen species (ROS) have recently been suggested as acting as intracellular second messengers. In this study, the effects of oxLp(a) on endothelia...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-013-3795-1
更新日期:2013-06-01 00:00:00
abstract::Stearoyl-CoA desaturase (SCD) is a regulatory enzyme in lipogenesis, catalyzing the rate-limiting step in the overall de novo synthesis of monounsaturated FA, mainly oleate and palmitoleate from stearoyl- and palmitoyl-CoA, respectively. Oleate and palmitoleate are the major monounsaturated FA of membrane phospholipid...
journal_title:Lipids
pub_type: 杂志文章,评审
doi:10.1007/s11745-004-1331-2
更新日期:2004-11-01 00:00:00
abstract::CLA has a range of biological properties, including effects on lipid metabolism and body composition in experimental animals. The prevalent isomer of CLA found in the human diet is 9c,11 t-CLA, and it is predominantly found in products containing fat from ruminant animals. This study investigated the effect of dietary...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-002-0950-y
更新日期:2002-07-01 00:00:00
abstract::The production of cardiac arrhythmias and the elevation of lysophosphatidylcholine level in the ischemic myocardium have been well-documented in a number of studies. However, the relationship between the production arrhythmias and the elevation of tissue lysophosphatidylcholine level was not reported. In this study, t...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02535301
更新日期:1988-01-01 00:00:00
abstract::Plasma lipidomic studies using high performance liquid chromatography and mass spectroscopy offer detailed insights into metabolic processes. Taking the example of the most abundant plasma lipid class (phosphatidylcholines) we used the rich phenotypic and lipidomic data from the ongoing San Antonio Family Heart Study ...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-013-3781-7
更新日期:2013-05-01 00:00:00
abstract::Diets that contain high levels of n-3 fatty acids from fish oil have been shown to significantly effect macrophage cytolytic capacity, tumor necrosis factor alpha production and eicosanoid production. The present study was undertaken to determine whether n-3 fatty acids from vegetable origin [linseed oil (LIN)] would ...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02536100
更新日期:1994-09-01 00:00:00
abstract::Male and female weanling rats that were born to dams fed a diet low in linolenic acid received diets of 15% lipids by weight containing 45% elaidic acid (as trielaidin) and 8.5% or 0.1% linolenic acid for 10 weeks. Four other groups, in which palmitic or oleic acid replaced elaidic acid in the diet, served as controls...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02536444
更新日期:1987-12-01 00:00:00
abstract::Cis-9-conjugated, trans-11-conjugated linoleic acid (CLA) is known for its positive activities on human health. The synthesis of cis-9, trans-11 CLA in mammary glands is generally thought to be catalyzed by stearoyl-CoA desaturase 1 (SCD1), but this has not been rigorously established. In this study, we hypothesized t...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1002/lipd.12077
更新日期:2018-06-01 00:00:00
abstract::This study analyses the effects of dietary lipid level and source on lipid absorption and metabolism in Senegalese sole (Solea senegalensis). Juvenile fish were fed 4 experimental diets containing either 100 % fish oil (FO) or 25 % FO and 75 % vegetable oil (VO; rapeseed, linseed and soybean oils) at two lipid levels ...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-015-4089-6
更新日期:2016-01-01 00:00:00
abstract::A fast and efficient shotgun lipidomics strategy was applied to analyze phospholipids (PL) in the oyster Crassostrea plicatula, including 29 species of phosphatidylcholine (PtdCho), 23 species of phosphatidylethanolamine (PtdEtn), 11 species of phosphatidylserine (PtdSer), 6 species of phosphatidylinositol (PtdIns), a...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-017-4305-7
更新日期:2017-12-01 00:00:00
abstract::The molecular species in mixtures of phospholipid hydroperoxides are difficult to separate and identify by typical chromatographic and mass spectrometric techniques. As reported by Havrilla and coworkers, silver ion coordination ion-spray mass spectrometry (CIS-MS) has proven to be a powerful technique for the identif...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-001-0841-2
更新日期:2001-11-01 00:00:00
abstract::After administering the equivalent of 1 g of eicosapentaenoic acid (EPA) in four different chemical forms, the kinetics of EPA incorporation into plasma triglycerides (TG) were compared by gas liquid chromatography on a capillary column following separation of the lipid fraction by thin layer chromatography. EPA incor...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02533970
更新日期:1987-10-01 00:00:00
abstract::The effect of apo very low density lipoprotein (apo VLDL) and apoprotein C-II on the phospholipase A1 activity associated with lipoprotein lipase (E.C.3.1.1.3) was studied using purified bovine milk lipoprotein lipase. The enzyme degraded 14C phosphatidylcholine (PC) to 14C 2-acyl lysophosphatidylcholine at a rate of ...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02540967
更新日期:1980-03-01 00:00:00
abstract::Salmon farmers are currently using high-energy feeds containing up to 35% fat; the fish's capability of fully utilizing these high-energy feeds has received little attention. Carnitine is an essential component in the process of mitochondrial fatty acid oxidation and, with the cooperation of two carnitine palmitoyltra...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-998-0289-4
更新日期:1998-09-01 00:00:00
abstract::Mice exposed to cold rely upon brown adipose tissue (BAT)-mediated nonshivering thermogenesis to generate body heat using dietary glucose and lipids from the liver and white adipose tissue. In this report, we investigate how cold exposure affects the PI3 K/Akt signaling cascade and the expression of genes involved in ...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-010-3422-3
更新日期:2010-06-01 00:00:00
abstract::An important enzyme involved in phospholipid turnover is the acyl-CoA: lysophosphatidylcholine acyltransferase (LPCAT). Here, we report characterization of a newly discovered human LPCAT (LPCAT3), which has distinct substrate preferences strikingly consistent with a role in phosphatidylcholine (PtdCho) remodeling and ...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-008-3233-y
更新日期:2008-10-01 00:00:00
abstract::In view of previous reports that the activity of the Mg(++)-dependent phosphatidic acid phosphatase in adipose tissues of rat and mouse is elevated in obesity, we attempted to assay this activity in biopsies of human omental adipose tissue obtained from normal-weight and morbidly obese subjects in connection with oper...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02544078
更新日期:1989-12-01 00:00:00
abstract::Three groups of male rats were fed either a corn oil-enriched diet (17%, w/w), a salmon oil-enriched diet (12.5%) supplemented with corn oil (4.5%) or a low-fat diet (4.4%) for eight wk to investigate the possible relationships between dietary fatty acids and lipid composition, and prostaglandin E2 level and phospholi...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02535665
更新日期:1988-07-01 00:00:00
abstract::To test the effects of eicosapentaenoic acid (EPA) infusion on pulmonary edema induced by coronary ligation and reperfusion, extravascular lung water (EVLW) was measured in situ by the thermal-dye double indicator dilution method in dogs. In the control group of five dogs, 30 mL of a 10% soybean oil emulsion was infus...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-997-0015-2
更新日期:1997-01-01 00:00:00
abstract::A recent study on the metabolism of 1-14C-alpha-linolenic acid in the guinea pig revealed that the fur had the highest specific activity of all tissues examined, 48 h after dosing. The present study investigated the pattern of tissue lipid labeling following an oral dose of 1-14C-linoleic acid after the animals had be...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-001-0715-7
更新日期:2001-03-01 00:00:00
abstract::The objective of the present studies was to examine the effect of dietary fat on the lipid composition of rat colonocytes and their utilization of short-chain fatty acids (SCFA). Rats were fed 14% beef fat, fish oil or safflower oil plus 2% corn oil in a semi-synthetic base diet for 4 wk. Colonocytes were isolated and...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02544340
更新日期:1990-06-01 00:00:00
abstract::Docosahexaenoic acid (DHA), a prominent long-chain fatty acid of the omega-3 family, is present at high amount in brain tissues, especially in membrane phospholipids. This polyunsaturated fatty acid is the precursor of various oxygenated lipid mediators involved in diverse physiological and pathophysiological processe...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1002/lipd.12006
更新日期:2018-01-01 00:00:00
abstract::The purpose of these experiments was to study the uptake and metabolism of exogenous triglyceride in the isolated perfused rabbit heart. When infused into the rabbit heart, [9,10-3H(N)]triolein was retained and incorporated into a lipid fraction that had the chromatographic mobility of authentic triolein. Incorporatio...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02538094
更新日期:1990-08-01 00:00:00
abstract::Rats were fed either a high alpha-linolenate diet or a high linoleate diet from weaning to 4 mon of age. Soybean oil was used as a control. Phospholipid compositions of erythrocytes from the three dietary groups were not significantly different. However, the difference in the alpha-linolenate (18:3n-3)/linoleate (18:2...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02535899
更新日期:1990-12-01 00:00:00
abstract::The fatty acid composition of cerebrosides of developing foetal human brain and regional variations, if any, during intrauterine life were studied. While palmitic and stearic acids were the predominant normal fatty acids throughout intrauterine life, long chain fatty acids, like lignoceric and nervonic acids, which we...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02533635
更新日期:1977-04-01 00:00:00
abstract::The effect of phosphatidylserine and sterol carrier proteins on cholesterol exchange was determined using an assay not requiring separation of donor and acceptor membrane vesicles. Sterol carrier protein-2 (SCP2, also called nonspecific lipid transfer protein), but not fatty acid binding protein (FABP, also called ste...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02544032
更新日期:1990-11-01 00:00:00