Abstract:
:In plants, two pathways are utilized for the synthesis of isopentenyl diphosphate (IPP), the universal precursor for isoprenoid biosynthesis. In this paper we review findings and observations made primarily with tobacco BY-2 cells (TBY-2), which have proven to be an excellent system in which to study the two biosynthetic pathways. A major advantage of these cells as an experimental system is their ability to readily take up specific inhibitors and stably- and/or radiolabeled precursors. This permits the functional elucidation of the role of isoprenoid end products and intermediates. Because TBY-2 cells undergo rapid cell division and can be synchronized within the cell cycle, they constitute a highly suitable test system for determination of those isoprenoids and intermediates that act as cell cycle inhibitors, thus giving an indication of which branches of the isoprenoid pathway are essential. Through chemical complementation; and use of precursors, intracellular compartmentation can be elucidated, as well as the extent to which the plastidial and cytosolic pathways contribute to the syntheses of specific groups of isoprenoids (e.g., sterols) via exchange of intermediates across membranes. These topics are discussed in the context of the pertinent literature.
journal_name
Lipidsjournal_title
Lipidsauthors
Hemmerlin A,Gerber E,Feldtrauer JF,Wentzinger L,Hartmann MA,Tritsch D,Hoeffler JF,Rohmer M,Bach TJdoi
10.1007/s11745-004-1289-0subject
Has Abstractpub_date
2004-08-01 00:00:00pages
723-35issue
8eissn
0024-4201issn
1558-9307journal_volume
39pub_type
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