cis-9,10-epoxystearic acid in human leukocytes: isolation and quantitative determination.

Abstract:

:Racemic cis-9,10-epoxystearic acid was isolated from total lipids of human leukocytes. Identification of the epoxy acid was based mainly on gas liquid chromatographic-mass spectrometric analysis and on its chemical conversion into threo-9,10-dihydroxystearic acid. A mass spectrometric method for quantitative determination of cis-9,10-epoxystearic acid using the tetradeuterated compound as internal standard was developed. Using this method, nonstimulated human leukocytes were found to contain 5.1 +/- 2.2 micrograms (SD) of cis-9,10-epoxystearic acid per 10(9) cells (n = 8). More than 90% of the epoxy acid occurred in its esterified form in leukocyte lipids.

journal_name

Lipids

journal_title

Lipids

authors

Fahlstadius P

doi

10.1007/BF02535645

subject

Has Abstract

pub_date

1988-11-01 00:00:00

pages

1015-8

issue

11

eissn

0024-4201

issn

1558-9307

journal_volume

23

pub_type

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