Abstract:
:Phospholipids are integral constituents of the milk fat globule membranes and they play a central role in infants' immune and inflammatory responses. A methodology employing liquid chromatography coupled with evaporative light scattering detector has been optimized and validated to quantify the major phospholipids classes in human milk. Phospholipids were extracted using chloroform and methanol and separated on C18 column. Repeatability, intermediate reproducibility, and recovery values were calculated and a large sample set of human milk analyzed. In human milk, phospholipid classes were quantified at concentrations of 0.6 mg/100 g for phosphatidylinositol; 4.2 mg/100 g for phosphatidylethanolamine, 0.4 mg/100 g for phosphatidylserine, 2.8 mg/100 g for phosphatidylcholine, and 4.6 mg/100 g for sphingomyelin. Their relative standard deviation of repeatability and intermediate reproducibility values ranging between 0.8 and 13.4 % and between 2.4 and 25.7 %, respectively. The recovery values ranged between 67 and 112 %. Finally, the validated method was used to quantify phospholipid classes in human milk collected from 50 volunteers 4 weeks postpartum providing absolute content of these lipids in a relatively large cohort. The average content of total phospholipids was 23.8 mg/100 g that corresponds to an estimated mean intake of 140 mg phospholipids/day in a 4-week old infant when exclusively breast-fed.
journal_name
Lipidsjournal_title
Lipidsauthors
Giuffrida F,Cruz-Hernandez C,Flück B,Tavazzi I,Thakkar SK,Destaillats F,Braun Mdoi
10.1007/s11745-013-3825-zsubject
Has Abstractpub_date
2013-10-01 00:00:00pages
1051-8issue
10eissn
0024-4201issn
1558-9307journal_volume
48pub_type
杂志文章相关文献
LIPIDS文献大全abstract::The content and positional distribution of CLA in TAG fractions of lamb tissues was examined with either preformed CLA or the linoleic acid precursor of CLA in the diet as experimental treatments. The CLA content of phospholipid (PL) from these tissues was also examined. Thirteen lambs were randomized to the following...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-002-0939-6
更新日期:2002-06-01 00:00:00
abstract::The molecular species of sphingoid bases were tagged with the fluorescent amino group reagent, 4-fluoro-7-nitrobenzofurazan (NBD-F). The NBD-sphingoid bases were analyzed by a highly selective and sensitive liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) technique capable of relia...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-013-3871-6
更新日期:2014-03-01 00:00:00
abstract::A systematic study of the influence of biological lipid peroxidation conditions on lipid hydroperoxide decomposition to thiobarbituric acid-reactive malondialdehyde is presented. A superoxide-dependent, iron-catalyzed peroxidation system was employed with xanthine oxidase plus hypoxanthine plus ferric iron-adenosine d...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02535249
更新日期:1989-02-01 00:00:00
abstract::The skin surface lipid of the dog has been reported to contain a high proportion of diol diesters having a lower mobility on thin layer chromatography than diesters from other species in spite of containing similar fatty acid and diol components. In the present study, dog skin surface lipid was separated by preparativ...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02533265
更新日期:1977-10-01 00:00:00
abstract::According to the model of "response to injury," the arterial endothelium is occasionally injured in hyperlipidemia, hypertension, diabetes mellitus and in other states known as risk factors. The ensuing inflammatory response is modulated by cytokines and growth factors, among them platelet-derived growth factor (PDGF)...
journal_title:Lipids
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1007/s11745-001-0689-5
更新日期:2001-01-01 00:00:00
abstract::Isolated rat hepatocytes were preincubated with egg lecithin liposomes containing [3H]dolichol and [3H]dolichyl ester, and the intracellular levels and distributions of these lipids were subsequently determined after incubation in a liposome-free medium. [3H]Dolichol was recovered initially mainly in microsomes, and n...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02536470
更新日期:1992-04-01 00:00:00
abstract::The all-cis-5,9,12-heptadecatrienoic acid was identified in the cellular slime mold Polysphondylium pallidum. The structural elucidation was accomplished by capillary gas chromatography, argentation thin-layer chromatography, and gas chromatography/mass spectrometry. This fatty acid has not been reported previously. ...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02522934
更新日期:1996-04-01 00:00:00
abstract::Orally administered dihomo-gamma-linolenic acid (DHLA) is well absorbed in man; it appears in blood after ca. 4 hr first as triglyceride ester and later as phospholipid. After sustained-dosing, DHLA penetrated membrane pools and all phospholipid components but, depending on the dosage, reached a metabolic equilibrium ...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02533869
更新日期:1979-02-01 00:00:00
abstract::Saturated fatty acids are the most abundant fatty acids in the brain, however, there has been some debate regarding the ability of intact dietary saturated fatty acids to be incorporated into the brain. In the present study, we use compound specific isotope analysis to measure the natural abundance carbon isotopic sig...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1002/lipd.12046
更新日期:2018-05-01 00:00:00
abstract::Recent reports show that a fatty meal can substantially increase the concentration of oxidized lipids in low density lipoprotein (LDL). Knowing the LDL-specific antioxidant effects of high density lipoprotein (HDL), we aimed to investigate whether HDL can modify the postprandial oxidative stress after a fatty meal. Su...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-014-3963-y
更新日期:2014-12-01 00:00:00
abstract::Recent work in our lab has shown that the chemoprotective fatty acid, conjugated linoleic acid (CLA), inhibits phorbol ester skin tumor promotion in mice. Because little is known about the deposition of CLA into tissues as well as its biological activity, this study compared the incorporation and biological activity o...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-997-0092-2
更新日期:1997-07-01 00:00:00
abstract::The activity of pyruvate dehydrogenase from freshly isolated mitochondria was shown to be dependent upon the nutritional and metabolic state of the animal prior to sacrifice, such that mitochondria from the livers of 48 hr starved, diabetic, or high fat fed rats had lower enzyme activity than normal, chow fed rats. Th...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02532317
更新日期:1975-12-01 00:00:00
abstract::Microtiter plate methods were developed for the enzymatic determination of serum total cholesterol (TC), high density lipoprotein cholesterol (HDL-C) and triglyceride (TG), and for the turbidometric determination of apolipoproteins. The micromethods resulted in accurate, precise values that were in good agreement with...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02535780
更新日期:1993-02-01 00:00:00
abstract::This paper represents the first synthesis, spectroscopic characterization, and antitumor evaluation of F-, N-, and S-containing C4alpha-FA derivatives of podophyllotoxin. In a synthetic strategy, a FA unit of 4-O-podophyllotoxinyl 12-hydroxyoctadec-Z-9-enoate 2, a derivative of podophyllotoxin, was functionalized at t...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-006-1397-x
更新日期:2005-04-01 00:00:00
abstract::Alteration of lipid constituents of cellular membranes has been proposed as a possible mechanism for cancer promotion by fumonisin B(1 )(FB(1)). To further investigate this hypothesis a dietary dosage which initiates and promotes liver cancer (250 mg FB(1)/kg) was fed to male Fischer rats for 21 days and the lipid com...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-007-3025-9
更新日期:2007-04-01 00:00:00
abstract::The objective of the present studies was to examine the effect of dietary fat on the lipid composition of rat colonocytes and their utilization of short-chain fatty acids (SCFA). Rats were fed 14% beef fat, fish oil or safflower oil plus 2% corn oil in a semi-synthetic base diet for 4 wk. Colonocytes were isolated and...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02544340
更新日期:1990-06-01 00:00:00
abstract::High-performance liquid chromatography-electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS) approaches have enabled high selectivity and sensitivity for the identification and quantification of glucosylceramide molecular species. Here we demonstrate that HPLC-ESI-MS/MS is an efficient method for character...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-012-3725-7
更新日期:2012-12-01 00:00:00
abstract::In this paper, evidence is presented on the capacity of Ehrlich ascites cells to synthesize in vitro monounsaturated fatty acids from radioactive palmitate. Localization of the double bond was determined by ozonolysis and subsequent reduction of the ozonides to aldesters followed by gas liquid chromatography. These re...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02534994
更新日期:1981-12-01 00:00:00
abstract::A one-carbon degradation of long-chain fatty acids, which was found to occur in the brains of rats in vivo, has been investigated in a brain microsomal fraction in vitro. Decarboxylation of the alpha-hydroxy acid, a possible intermediate product between the substrate and the next shorter acid, in the presence of brain...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02531201
更新日期:1968-05-01 00:00:00
abstract::In vivo intestinal perfusion was used to follow the absorption of three different choline glycerophospholipids (CGP) in guinea pig. These included 1-[3H]palmitoyl-2-acyl-sn-glycero-3-phosphocholine (diacyl-GPC), 1-[3H]-O-hexadecyl-2-acyl-sn-glycero-3-phosphocholine (alkylacyl-GPC) and 1,2-di-O-hexadecyl-sn-glycero-3-p...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02534872
更新日期:1987-01-01 00:00:00
abstract::[1-14C]alpha-Linolenic acid was incubated with a particulate fraction of homogenate of leaves of the meadow buttercup (Ranunculus acris L.). The main product was a divinyl ether fatty acid, which was identified as 12-[1'(Z),3'(Z)-hexadienyloxy]-9(Z),11(E)-dodecadienoic acid. Addition of glutathione peroxidase and redu...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-998-0306-7
更新日期:1998-11-01 00:00:00
abstract::The triacylglycerols of very low density lipoproteins (VLDL-TG) were analyzed in samples from normal subjects and patients with Frederickson's Type III and Type IV hyperlipoproteinemia. VLDL were obtained by conventional ultracentrifugation, and the triacylglycerols were isolated by thin-layer chromatography (TLC). Re...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02534528
更新日期:1984-09-01 00:00:00
abstract::The effect of simvastatin, a hypocholesterolemic drug, on the biosynthesis of arachidonic acid was studied in obese and lean Zucker rats. After administration of 2 mg/kg body weight/d for 13 d, delta 6 and delta 5 desaturase activities were measured in liver microsomes at two substrate concentrations. In untreated rat...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02536363
更新日期:1993-01-01 00:00:00
abstract::Seventy-two surgically obtained Dupuytren's disease palmar-fat (DDPF) specimens and 18 location-matched specimens from patients not suffering from this disease (controls) were studied for their total lipid composition and de novo lipogenic activity. Incubation of "DDPF" with 1-[14C]acetate in oxygen produced [14C]palm...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02537234
更新日期:1983-05-01 00:00:00
abstract::Guar gum has a well-recognized hypolipidemic effect. This effect is thought to be due to the physicochemical properties of guar gum, which may cause changes in adsorption of lipids or the viscosity of the intestinal contents. Guar gum is a non-specific absorption inhibitor of any type of lipid-soluble compound. Perman...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-011-3570-0
更新日期:2011-08-01 00:00:00
abstract::Acetate supplementation increases plasma acetate, brain acetyl-CoA, histone acetylation, phosphocreatine levels, and is anti-inflammatory in models of neuroinflammation and neuroborreliosis. Although radiolabeled acetate is incorporated into the cellular lipid pools, the effect that acetate supplementation has on lipi...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-014-3911-x
更新日期:2014-07-01 00:00:00
abstract::The synthesis of phosphoglycerides is reviewed. Emphasis is placed upon precursor and intermediate compounds, phosphatidic acid, phosphatidyl choline, phosphatidyl ethanolamine, phosphatidyl serine, monoacylphosphoglycerides, purification and proof of structure. Suggestions are made for large scale synthesis of phosph...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02531964
更新日期:1972-09-01 00:00:00
abstract::A methodology combining finger-pricked blood sampling, microwave accelerated fatty acid assay, fast gas chromatography data acquisition, and automated data processing was developed, evaluated and applied to a population study. Finger-pricked blood was collected on filter paper previously impregnated with 0.05 mg of th...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-014-3918-3
更新日期:2014-08-01 00:00:00
abstract::Using the spectrofluorimetric method described by Wittenauer et al. [Wittenauer, L.A., Shirai, K., Jackson, R.L., and Johnson, J.D. (1984) Biochem. Biophys. Res. Commun. 118, 894-901] for phospholipase A2 (PLA2) measurement, we have detected a phospholipase activity in Ailsa Craig and in mutant rin tomatoes at their n...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02537801
更新日期:1995-08-01 00:00:00
abstract::Rats were fed semipurified diets containing olive oil or partially hydrogenated corn oil at the 5 or 20% level for ca. 30 days. These fat diets contained the same amount of octadecenoate but differed in the geometry with respect to each fat level. Contents of t-18:1 were 26% and 41% of total fatty acids, respectively....
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02534500
更新日期:1984-02-01 00:00:00