Fast transmethylation of total lipids in dried blood by microwave irradiation and its application to a population study.

Abstract:

:A methodology combining finger-pricked blood sampling, microwave accelerated fatty acid assay, fast gas chromatography data acquisition, and automated data processing was developed, evaluated and applied to a population study. Finger-pricked blood was collected on filter paper previously impregnated with 0.05 mg of the antioxidant butylated hydroxytoluene and air-dried at room temperature. Transmethylation was accelerated by microwave irradiation in an explosion-proof multimode microwave reaction system. The chemical procedure was based on a one-step direct transmethylation procedure catalyzed by acetyl chloride. The short-term stability of PUFA in blood dried on filter paper and storage at room temperature was examined using venous blood. The recoveries ranged from 97 to 101 % for the categorized fatty acids as well as the ratios of n-6 to n-3 PUFA and the n-3 % highly unsaturated fatty acid. Specifically, recoveries were 99, 98, 97, and 97 % for linoleic acid (18:2n-6), arachidonic acid (ARA), α-linolenic acid (ALA), and docosahexaenoic acid (DHA), respectively. The mol% (mean ± SD, 95 % confidence interval) of fatty acid composition in subjects from the population study was determined as 36.2 ± 3.8 (35.8, 36.7), 23.2 ± 3.0 (22.8, 23.5), 36.8 ± 3.5 (36.4, 37.2) and 3.79 ± 1.0 (3.68, 3.91) for the saturated, monounsaturated, n-6 and n-3 PUFA, respectively. Individually, the mean mol% (95 % CI) was 22.6 (22.3, 22.9) for 18:2n-6, 9.5 (9.3, 9.7) for ARA, 0.51 (0.49, 0.53) for ALA, 0.42 (0.38, 0.47) for eicosapentaenoic acid (EPA), and 1.67 (1.61, 1.73) for DHA. This methodology provides an accelerated yet high-efficiency, chemically safe, and temperature-controlled transmethylation, with diverse laboratory applications including population studies.

journal_name

Lipids

journal_title

Lipids

authors

Lin YH,Hanson JA,Strandjord SE,Salem NM,Dretsch MN,Haub MD,Hibbeln JR

doi

10.1007/s11745-014-3918-3

subject

Has Abstract

pub_date

2014-08-01 00:00:00

pages

839-51

issue

8

eissn

0024-4201

issn

1558-9307

journal_volume

49

pub_type

杂志文章

相关文献

LIPIDS文献大全
  • Cardiopathogenicity of rapeseed oils and oil blends differing in erucic, linoleic, and linolenic acid content.

    abstract::Male Wistar rats were fed semipurifed diets containing 20% fat for 25 weeks. Ten different oils or oil blends were employed, including rapessed oils, simulated rapeseed-type oils, and modified rapeseed-type oils. Safflower, soybean, and hydrogenated coconut oils served as control oils. Histopathological examination of...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02532900

    authors: McCutcheon JS,Umermura T,Bhatnager MK,Walker BL

    更新日期:1976-07-01 00:00:00

  • A maternal high fat diet has long-lasting effects on skeletal muscle lipid and PLIN protein content in rat offspring at young adulthood.

    abstract::A maternal high fat diet (HFD) can have adverse effects on skeletal muscle development. Skeletal muscle PLIN proteins (PLIN2, 3 and 5) are thought to play critical roles in lipid metabolism, however effects of HFD on PLIN and lipases (HSL, ATGL, CGI-58) in mothers as well as their offspring have yet to be investigated...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/s11745-014-3985-5

    authors: MacPherson RE,Castelli LM,Miotto PM,Frendo-Cumbo S,Milburn A,Roy BD,LeBlanc PJ,Ward WE,Peters SJ

    更新日期:2015-02-01 00:00:00

  • Comparison of ultracentrifugation and gel filtration for the isolation of bovine lipoproteins.

    abstract::Lipoproteins from the plasma of three nonlactating Holstein cows were isolated using either preparative ultracentrifugation or gel filtration chromatography. Lipoprotein classes obtained by ultracentrifugation were very low density plus chylomicra, less than 1.006 g/ml; low density, 1.007-1.039 g/ml; high density1, 1....

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02534638

    authors: Grummer RR,Davis CL,Hegarty HM

    更新日期:1983-11-01 00:00:00

  • Phospholipid metabolism in Neisseria gonorrhoeae: phospholipid hydrolysis in nongrowing cells.

    abstract::Hydrolysis of cell envelope phospholipids was demonstrated in cells of both autolytic and nonautolytic strains of Neisseria gonorrhoeae that were labeled during growth in the presence of [3H] acetate. The label incorporated into the cellular phospholipids was located exclusively in the fatty acid acyl side chains. Lab...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02533897

    authors: Cacciapuoti AF,Wegener WS,Morse SA

    更新日期:1979-08-01 00:00:00

  • Effect of synthetic phospholipids on platelet aggregation and serotonin release.

    abstract::1-O-Hexadecyl-2-O-acetyl-sn-glycero-3-phosphocholine (platelet-activating factor, PAF) is known to stimulate platelet aggregation and serotonin release in concentrations ranging from 10(-10)-10(-5) M. Since a variety of synthetic PAF analogues are potent antineoplastic agents in vitro and in vivo, it was the aim of th...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02535546

    authors: Söling U,Eibl H,Nagel GA,Unger C

    更新日期:1987-11-01 00:00:00

  • Activation of acyl-CoA cholesterol acyltransferase: redistribution in microsomal fragments of cholesterol and its facilitated movement by methyl-beta-cyclodextrin.

    abstract::Acyl-CoA cholesterol acyltransferase (ACAT) (EC 2.3.1.26) in the yolk sac membrane of chicken eggs plays an important role in the transport of lipids, which serve as both structural components and as an energy source during embryogenesis. ACAT from the yolk sac membrane of chicken eggs 16 d after fertilization has hig...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/s11745-999-0362-z

    authors: Cheng D,Tipton CL

    更新日期:1999-03-01 00:00:00

  • Types of oilseed and adipose tissue influence the composition and relationships of polyunsaturated fatty acid biohydrogenation products in steers fed a grass hay diet.

    abstract::The current study evaluated the composition and relationships of polyunsaturated fatty acid biohydrogenation products (PUFA-BHP) from the perirenal (PRF) and subcutaneous fat (SCF) of yearling steers fed a 70 % grass hay diet with concentrates containing either sunflower-seed (SS) or flaxseed (FS). Analysis of varianc...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/s11745-013-3876-1

    authors: Mapiye C,Aalhus JL,Turner TD,Rolland DC,Basarab JA,Baron VS,McAllister TA,Block HC,Proctor SD,Dugan ME

    更新日期:2014-03-01 00:00:00

  • Effects of dietary vitamin E, selenium, and polyunsaturated fats on in vivo lipid peroxidation in the rat as measured by pentane production.

    abstract::Starting at 21 days of age, groups of six rats each were fed a basal Torula yeast diet supplemented with 0.4% L-methionine and varying amounts of vitamin E as dl-alpha tocopherol acetate, selenium as sodium selenite, and with either 10% stripped corn oil, stripped lard, or coconut oil. By 7 wk, pentane production by r...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02533708

    authors: Dillard CJ,Litov RE,Tappel AL

    更新日期:1978-06-01 00:00:00

  • The effect of hibernation on the positional distribution of ethanolamineglycerophospholipid fatty acids in hamster brain membranes.

    abstract::Microsomal and myelin membrane fractions were prepared from the brains of warm-adapted (room temperature) and hibernating Syrian Hamsters (Mesocricetus auratus). Ethanolamineglycerophospholipids were isolated and subjected to a fractionation scheme to separate the fatty acids of the plasmenylethanolamine and the phosp...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02533441

    authors: Blaker WD,Moscatelli EA

    更新日期:1979-12-01 00:00:00

  • A rapid method for the determination of vitamin E forms in tissues and diet by high-performance liquid chromatography using a normal-phase diol column.

    abstract::This paper describes a simple method for the analysis of tocopherols in tissues by which frozen tissues-70 degrees C were pulverized at dry ice temperatures (-70 degrees C) and immediately extracted with hexane. There was no need to remove the coeluting lipids from tissues by saponification, since at that level of neu...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/s11745-997-0040-1

    authors: Kramer JK,Blais L,Fouchard RC,Melnyk RA,Kallury KM

    更新日期:1997-03-01 00:00:00

  • Dietary lipid level induced antioxidant response in Manchurian trout, Brachymystax lenok (Pallas) larvae.

    abstract::This study was designed to determine the nutritional lipid requirement of Manchurian trout and to investigate the effects of lipid concentrations on the antioxidant status in larvae with experimental diets with different lipid levels. Oxidative stress differences between different organs and tissues were also assessed...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/s11745-009-3313-7

    authors: Zhang H,Mu Z,Xu L,Xu G,Liu M,Shan A

    更新日期:2009-07-01 00:00:00

  • Effects of high levels of dietary fats on the growth of azaserine-induced foci in the rat pancreas.

    abstract::Azaserine induced two phenotypically different populations of foci, namely, acidophilic and basophilic foci. The effects of dietary modification during the post-initiation phase of carcinogenesis were examined. A diet of 20% (w/w) unsaturated fat (unsat) compared to a 20% saturated fat (sat) diet or a control diet (5%...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02536413

    authors: Roebuck BD

    更新日期:1986-04-01 00:00:00

  • Supplementation with a pine bark extract rich in polyphenols increases plasma antioxidant capacity and alters the plasma lipoprotein profile.

    abstract::Pycnogenol (PYC), an extract of French maritime pine bark (Pinus pinaster), is a potent antioxidant with potential health benefits. Its bioavailabilty has previously been shown by urinary excretion studies of constituents and metabolites of PYC. The aim of this study was to test the effect of PYC supplementation on me...

    journal_title:Lipids

    pub_type: 临床试验,杂志文章

    doi:10.1007/s11745-006-0982-3

    authors: Devaraj S,Vega-López S,Kaul N,Schönlau F,Rohdewald P,Jialal I

    更新日期:2002-10-01 00:00:00

  • Comparison of antioxidant activities of tocol and its methyl derivatives.

    abstract::In tests with purified menhaden oil at 37C and with squalene at 37C and 50C, unsubstituted tocol, 7,8-dimethyl tocol (gamma-tocol), and 8-methyl tocol (sigma-tocol) were superior antioxidants to 5,7-dimethyl tocol and 5,7,8-trimethyl tocol (alpha-tocol). In contrast to previous reports, there were no differences in th...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02530934

    authors: Olcott HS,Van der Veen J

    更新日期:1968-07-01 00:00:00

  • Serum high density lipoprotein and its relationship to cardiovascular disease risk factor variables in children--the Bogalusa heart study.

    abstract::Serum high density lipoprotein is increasingly recognized as a negative risk for cardiovascular disease. The distribution and interrelationship of serum lipids, lipoproteins, anthropometric measurements and blood pressures were determined in some 5,000 children. Children had mean +/- S.D. alpha-lipoprotein cholesterol...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02533576

    authors: Berenson GS,Srinivasan SR,Frerichs RR,Webber LS

    更新日期:1979-01-01 00:00:00

  • Intestinal absorption of ester and ether glycerophospholipids in guinea pig. Role of a phospholipase A2 from brush border membrane.

    abstract::In vivo intestinal perfusion was used to follow the absorption of three different choline glycerophospholipids (CGP) in guinea pig. These included 1-[3H]palmitoyl-2-acyl-sn-glycero-3-phosphocholine (diacyl-GPC), 1-[3H]-O-hexadecyl-2-acyl-sn-glycero-3-phosphocholine (alkylacyl-GPC) and 1,2-di-O-hexadecyl-sn-glycero-3-p...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02534872

    authors: Diagne A,Mitjavila S,Fauvel J,Chap H,Douste-Blazy L

    更新日期:1987-01-01 00:00:00

  • The lipid components of white potato tubers (Solanum tuberosum).

    abstract::Four Canadian varieties of potatoes were examined for their lipid composition. Lipids, extracted with chloroformmethanol, were shown by TLC and column chromatography to consist of 16.5% neutral lipids, 45.5% phospholipids and 38.1% glycolipids. Among the phospholipids and glycolipids, phosphatidyl choline, phosphatidy...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02530889

    authors: Lepage M

    更新日期:1968-11-01 00:00:00

  • Lymphatic transport of α-linolenic acid and its conversion to long chain n-3 fatty acids in rats fed microemulsions of linseed oil.

    abstract::In the present study we evaluated the uptake of ALA and its conversion to EPA + DHA in rats given linseed oil (LSO) in native form or as a microemulsion in whey protein or in lipoid. In a single oral dose study in which rats maintained on rodent pellets deficient in ω-3 fatty acids were intubated with 0.35 g LSO in li...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/s11745-013-3873-4

    authors: Sugasini D,Devaraj VC,Ramesh M,Lokesh BR

    更新日期:2014-03-01 00:00:00

  • The effect of modest vitamin E supplementation on lipid peroxidation products and other cardiovascular risk factors in diabetic patients.

    abstract::Among many factors, elevated lipids and lipid peroxide levels in blood are major risk factors in the development of cardiovascular disease in diabetic patients. This study has examined whether oral supplementation of vitamin E, an antioxidant, has any effect on blood lipid peroxidation products (LP) and lipid profile ...

    journal_title:Lipids

    pub_type: 临床试验,杂志文章

    doi:10.1007/BF02637057

    authors: Jain SK,McVie R,Jaramillo JJ,Palmer M,Smith T,Meachum ZD,Little RL

    更新日期:1996-03-01 00:00:00

  • Human platelets release a paf-acether: acetylhydrolase similar to that in plasma.

    abstract::Intact washed human platelets aggregated in response to paf-acether (paf) and did not metabolize [3H]paf at concentrations up to 10 nM. However, when platelets were lysed by exposure to pH 9.5, resulting in 37.5 +/- 2.5% (mean +/- SD, n = 3) lactic dehydrogenase (LDH) release, 20.5 +/- 5.7% of the radioactivity was de...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02536639

    authors: Korth R,Bidault J,Palmantier R,Benveniste J,Ninio E

    更新日期:1993-03-01 00:00:00

  • Enrichment of LDL with EPA and DHA decreased oxidized LDL-induced apoptosis in U937 cells.

    abstract::Oxidized LDL (oxLDL) may contribute to the accumulation of apoptotic cells in atherosclerotic plaques. Although it is well established in monophasic chemical systems that the highly unsaturated EPA and DHA will oxidize more readily than FA that contain fewer double bonds, our previous studies showed that enrichment of...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/s11745-002-0962-7

    authors: Wu T,Geigerman C,Lee YS,Wander RC

    更新日期:2002-08-01 00:00:00

  • Membrane docosahexaenoic acid vs. eicosapentaenoic acid and the beating function of the cardiomyocyte and its regulation through the adrenergic receptors.

    abstract::The beta-adrenergic system in cardiac muscle cells is influenced by the n-3 polyunsaturated fatty acid (PUFA) content in cell membranes. This study deals with the specific effect of docosahexaenoic acid (DHA) as compared to other n-3 PUFA, without modification of the arachidonic acid content. Increasing the DHA conten...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:

    authors: Grynberg A,Fournier A,Sergiel JP,Athias P

    更新日期:1996-03-01 00:00:00

  • Glucose Uptake and Triacylglycerol Synthesis Are Increased in Barth Syndrome Lymphoblasts.

    abstract::Barth syndrome (BTHS) is an X-linked genetic disease resulting in loss of cardiolipin (Ptd2Gro). Patients may be predisposed to hypoglycemia and exhibit increases in whole-body glucose disposal rates and a higher fat mass percentage. We examined the reasons for this in BTHS lymphoblasts. BTHS lymphoblasts exhibited a ...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/s11745-017-4232-7

    authors: Mejia EM,Zinko JC,Hauff KD,Xu FY,Ravandi A,Hatch GM

    更新日期:2017-02-01 00:00:00

  • Comparison of separations of fatty acids from fish products using a 30-m Supelcowax-10 and a 100-m SP-2560 column.

    abstract::Commercial fish oils and foods containing fish may contain trans and/or isomerized fatty acids (FA) produced during processing or as part of prepared foods. The current American Oil Chemists' Society (AOCS) official method for marine oils (method Ce 1i-07) is based on separation by use of poly(ethylene glycol) (PEG) c...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/s11745-011-3645-y

    authors: Santercole V,Delmonte P,Kramer JK

    更新日期:2012-03-01 00:00:00

  • Positional specificity of trans fatty acids in fetal lecithin.

    abstract::Differences in the positional incorporation of 9-trans[1-(14)C] octadecenoic (elaidic) and 9-trans,12-trans[1-(14)C] octadecadienoic (linoelaidic) acids in fetal lecithin of rats were demonstrated. On the 20th day of gestation, a 14C-labeled albumin complex of elaidic or linoelaidic acid was injected into the jugular ...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02535045

    authors: Moore CE,Dhopeshwarkar GA

    更新日期:1981-07-01 00:00:00

  • 2-Methoxylated FA Display Unusual Antibacterial Activity Towards Clinical Isolates of Methicillin-Resistant Staphylococcus aureus (CIMRSA) and Escherichia coli.

    abstract::The naturally occurring (6Z)-(±)-2-methoxy-6-hexadecenoic acid (1) and (6Z)-(±)-2-methoxy-6-octadecenoic acid (2) were synthesized in 7-8 steps with 38 and 13% overall yields, respectively, by using an acetylide coupling approach, which made it possible to obtain a 100% cis-stereochemistry for the double bonds. In a s...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/s11745-017-4262-1

    authors: Carballeira NM,Montano N,Morales C,Mooney J,Torres X,Díaz D,Sanabria-Rios DJ

    更新日期:2017-06-01 00:00:00

  • The effects of trans fatty acids on fatty acyl delta 5 desaturation by human skin fibroblasts.

    abstract::The effectiveness of different fatty acids as inhibitors of fatty acyl delta 5 desaturation activity in human skin fibroblasts has been investigated. When incubated with 2.25 microM [14C] eicosatrienoate (20:3 omega 6) in otherwise lipid-free medium, these cells rapidly incorporate the radiolabeled fatty acid into cel...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02534517

    authors: Rosenthal MD,Doloresco MA

    更新日期:1984-11-01 00:00:00

  • Sterol composition of phaeodactylum tricornutum as influenced by growth temperature and light spectral quality.

    abstract::In a detailed sterol analysis of the marine diatom Phaeodactylum tricornutum, free sterols as well as esterified and glycosylated conjugates were found. When the alga was grown under standard conditions (i.e., at 13 degrees C under white light), 64% of total sterols were steryl glycosides. In all sterol classes, excep...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/BF02522694

    authors: Véron B,Billard C,Dauguet JC,Hartmann MA

    更新日期:1996-09-01 00:00:00

  • Kocuria Bacterial Isolates from Radioactive Springs of Jáchymov spa (Joachimsthal) as Sources of Polyunsaturated Fatty Acids.

    abstract::Four bacterial isolates, which produced polyunsaturated fatty acids (PUFA), were isolated from water samples of radioactive springs collected from Jáchymov spa. Jáchymov (Sankt Joachimsthal) is a city in northwestern Bohemia, where Marie and Pierre Curie isolated radium in 1898 from the mineral uraninite. To date, fou...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1002/lipd.12136

    authors: Řezanka T,Gharwalová L,Nováková G,Kolouchová I,Uhlík O,Sigler K

    更新日期:2019-02-01 00:00:00

  • Glucose-sensitive liposomes incorporating hydrophobically modified glucose oxidase.

    abstract::Glucose-sensitive liposomes were prepared by incorporating hydrophobically modified glucose oxidase (EC 1.1.3.4.) into the liposomal bilayer of dioleoylphosphatidylethanolamine and cholesteryl hemisuccinate. For the release test, calcein, a fluorescence marker, was entrapped in the liposomes. The liposomes were stable...

    journal_title:Lipids

    pub_type: 杂志文章

    doi:10.1007/s11745-008-3223-0

    authors: Jo SM,Lee HY,Kim JC

    更新日期:2008-10-01 00:00:00