A Simplified Gas Chromatographic Fatty-Acid Analysis by the Direct Saponification/Methylation Procedure and Its Application on Wild Tuna Larvae.

abstract：:The activity of pyruvate dehydrogenase from freshly isolated mitochondria was shown to be dependent upon the nutritional and metabolic state of the animal prior to sacrifice, such that mitochondria from the livers of 48 hr starved, diabetic, or high fat fed rats had lower enzyme activity than normal, chow fed rats. Th...

journal_title：Lipids

authors： Mapes JP,Harris RA

更新日期：1975-12-01 00:00:00

abstract：:Microtiter plate methods were developed for the enzymatic determination of serum total cholesterol (TC), high density lipoprotein cholesterol (HDL-C) and triglyceride (TG), and for the turbidometric determination of apolipoproteins. The micromethods resulted in accurate, precise values that were in good agreement with...

journal_title：Lipids

authors： Shireman RB,Durieux J

更新日期：1993-02-01 00:00:00

abstract：:Several water soluble and organic solvent soluble fluorescent peaks were observed in extracts of uteri from vitamin E deficient or supplemented rats following Sephadex column chromatography and spectrophotofluorometric analysis. Levels of one of the organic and two of the water soluble fluorescent peaks were found to ...

journal_title：Lipids

authors： Csallany AS,Ayaz KL,Menken BZ

更新日期：1984-12-01 00:00:00

abstract：:The catfish, Ictalurus punctatus, is an important model for studying the biochemical mechanisms of taste at the peripheral level. The type, amount and metabolic activity of the lipids within this tissue play important roles in taste transduction by forming the matrix in which the receptors for taste stimuli are imbedd...

journal_title：Lipids

authors： Rabinowitz JL,Huque T,Brand JG,Bayley DL

更新日期：1990-04-01 00:00:00

abstract：:The effects of increasing concentrations of eicosapentaenoic acid (20:5n-3; EPA) and oleic acid (18:1n-9; OA) on esterification to triacylglycerols (TG) and phospholipids (PL), and the relationship to formation and secretion of the very low density lipoproteins (VLDL) were compared in the isolated perfused rat liver. ...

journal_title：Lipids

authors： Zhang ZJ,Wilcox HG,Castellani L,Fungwe TV,Elam MB,Heimberg M

更新日期：1993-05-01 00:00:00

abstract：:During our ongoing project on the biosynthesis of R-(+)-octane-1,3-diol the metabolism of linoleic acid was investigated in stored apples after injection of [1-14C]-, [9,10,12,13-3H]-,13C18- and unlabeled substrates. After different incubation periods the products were analyzed by gas chromatography-mass spectroscopy ...

journal_title：Lipids

authors： Beuerle T,Schwab W

更新日期：1999-04-01 00:00:00

abstract：:In previous studies, zinc-deficient rats force-fed a diet with coconut oil as the major dietary fat developed a fatty liver, whereas zinc-deficient rats force-fed a diet with linseed oil did not. The present study was conducted to elucidate the reason for this phenomenon. In a bifactorial experiment, rats were fed zin...

journal_title：Lipids

authors： Eder K,Kirchgessner M

更新日期：1995-01-01 00:00:00

abstract：:A methodology combining finger-pricked blood sampling, microwave accelerated fatty acid assay, fast gas chromatography data acquisition, and automated data processing was developed, evaluated and applied to a population study. Finger-pricked blood was collected on filter paper previously impregnated with 0.05 mg of th...

journal_title：Lipids

authors： Lin YH,Hanson JA,Strandjord SE,Salem NM,Dretsch MN,Haub MD,Hibbeln JR

更新日期：2014-08-01 00:00:00

abstract：:We have investigated the regulation of cholesterol synthesis in isolated human small intestine epithelial cells (enterocytes). It was established that the amount of cholesterol synthesized increased linearly with the incubation time and the number of cells in the incubation mixture; the synthesis was suppressed by 7-k...

journal_title：Lipids

authors： Sviridov DD,Safonova IG,Talalaev AG,Repin VS,Smirnov VN

更新日期：1986-12-01 00:00:00

abstract：:We examined growth performance and the lipid content in juvenile cobia, Rachycentron canadum, fed a taurine supplemented (1.5 %), plant protein based diet with two fish oil replacements. The first fish oil replacement was a thraustochytrid meal (TM + SOY) plus soybean oil (~9 % CL) and the second was a canola oil supp...

journal_title：Lipids

authors： Watson AM,Barrows FT,Place AR

更新日期：2013-09-01 00:00:00

abstract：:[1-14C]alpha-Linolenic acid was incubated with a particulate fraction of homogenate of leaves of the meadow buttercup (Ranunculus acris L.). The main product was a divinyl ether fatty acid, which was identified as 12-[1'(Z),3'(Z)-hexadienyloxy]-9(Z),11(E)-dodecadienoic acid. Addition of glutathione peroxidase and redu...

journal_title：Lipids

authors： Hamberg M

更新日期：1998-11-01 00:00:00

abstract：:Glucose-sensitive liposomes were prepared by incorporating hydrophobically modified glucose oxidase (EC 1.1.3.4.) into the liposomal bilayer of dioleoylphosphatidylethanolamine and cholesteryl hemisuccinate. For the release test, calcein, a fluorescence marker, was entrapped in the liposomes. The liposomes were stable...

journal_title：Lipids

authors： Jo SM,Lee HY,Kim JC

更新日期：2008-10-01 00:00:00

abstract：:Earlier studies showed that during the first 20 to 25 seconds of aggregation induced by thrombin (0.1 U/mL) or adenosine diphosphate (ADP) (2 microM) of rabbit or human platelets prelabeled with [3H]palmitic acid, labeled lipid became associated with the cytoskeleton (isolated after lysis with 1% Triton X-100, 5 mM EG...

journal_title：Lipids

authors： Packham MA,Guccione MA,Bryant NL,Livne A

更新日期：1991-07-01 00:00:00

abstract：:The degradation of alpha-tocopherol and the formation of alpha-tocopherol and triacylglycerol oxidation products at high temperatures (150-250 degrees C) over a heating period (0-4 h) for a model system ranging between triolein and tripalmitin were modeled by use of an experimental design. The oxidation products of al...

journal_title：Lipids

authors： Verleyen T,Kamal-Eldin A,Dobarganes C,Verhe R,Dewettinck K,Huyghebaert A

更新日期：2001-07-01 00:00:00

abstract：:The presence of leptin receptors in white adipose tissue (WAT) suggests a type of peripheral control during the development of obesity and other metabolic disorders. Both diet composition and exercise influence serum leptin; however, the effect of their combination on long-term WAT leptin metabolism is unknown. In thi...

journal_title：Lipids

authors： de Queiroz KB,Guimarães JB,Coimbra CC,Rodovalho GV,Carneiro CM,Evangelista EA,Guerra-Sá R

更新日期：2014-01-01 00:00:00

abstract：:In an attempt to correlate the incorporation of fatty acids (FA) of different chain length into liver and biliary lipids, isolated rat livers were perfused for 2 h with Krebs-Ringer bicarbonate containing 1% albumin and 10 mumol of [1-14C]-labeled FA: C2, C8, C10, C12, C16, and C18:1. One to 1.36 mumol of medium-chain...

journal_title：Lipids

authors： Rubin M,Pakula R,Gilat T,Tietz A

更新日期：1999-06-01 00:00:00

abstract：:The separation of lecithin derivatives based on their fatty acid substituents has been investigated. Several synthetic and natural lecithins were converted to their corresponding dimethylphosphatidates by hydrolysis with phospholipase D (phosphatidylcholine phospholipase D (phosphatidylcholine phosphatidohydrolase, EC...

journal_title：Lipids

authors： Wurster CF Jr,Copenhaver JH Jr

更新日期：1966-11-01 00:00:00

abstract：:A one-carbon degradation of long-chain fatty acids, which was found to occur in the brains of rats in vivo, has been investigated in a brain microsomal fraction in vitro. Decarboxylation of the alpha-hydroxy acid, a possible intermediate product between the substrate and the next shorter acid, in the presence of brain...

journal_title：Lipids

authors： Macdonald RC,Mead JF

更新日期：1968-05-01 00:00:00

abstract：:Primary cultured hepatocytes from normal mice and mice with Sarcoma 180 were characterized. The viability of freshly isolated hepatocytes from both sources was over 90% and the cells had a relatively stable population of DNA for a minimum of three days. After incubation with (3H)leucine, the syntheses and secretions o...

journal_title：Lipids

authors： Masuno H,Okuda H

更新日期：1985-07-01 00:00:00

abstract：:Salmon farmers are currently using high-energy feeds containing up to 35% fat; the fish's capability of fully utilizing these high-energy feeds has received little attention. Carnitine is an essential component in the process of mitochondrial fatty acid oxidation and, with the cooperation of two carnitine palmitoyltra...

journal_title：Lipids

authors： Frøyland L,Madsen L,Eckhoff KM,Lie O,Berge RK

更新日期：1998-09-01 00:00:00

abstract：:Triacylglycerols containing linoleate and linolenate found in vegetable oils were synthesized in gram quantities for oxidation studies. Two acylation methods were examined to convert diacylglycerols or monoacylglycerols to the desired triacylglycerols. Acylations with fatty acid and 1,1'-dicyclohexylcarbodiimide in th...

journal_title：Lipids

authors： Awl RA,Frankel EN,Weisleder D

更新日期：1989-10-01 00:00:00

abstract：:Human chylomicron triglyceride (TG) kinetics has been difficult to determine directly owing to technical limitations. This report describes a new method for studying chylomicron metabolism. Healthy volunteers (n = 10) sipped a drink providing 175 mg fat x kg(-1) h(-1) for 7.5 h to produce a steady-state chylomicronemi...

journal_title：Lipids

authors： Park Y,Damron BD,Miles JM,Harris WS

更新日期：2001-02-01 00:00:00

abstract：:This work reports the effect of the apolipoproteins A-I and A-II (apoA-I and apoA-II) on the release of tumor necrosis factor (TNF)-alpha, interleukin (IL)-1beta, IL-8, and IL-1 receptor antagonist (IL-1Ra) and on the oxidative burst of human neutrophils. By themselves, apoA-I and apoA-II do not affect the basal liber...

journal_title：Lipids

authors： Furlaneto CJ,Ribeiro FP,Hatanaka E,Souza GM,Cassatella MA,Campa A

更新日期：2002-09-01 00:00:00

abstract：:Diets that contain high levels of n-3 fatty acids from fish oil have been shown to significantly effect macrophage cytolytic capacity, tumor necrosis factor alpha production and eicosanoid production. The present study was undertaken to determine whether n-3 fatty acids from vegetable origin [linseed oil (LIN)] would ...

journal_title：Lipids

authors： Hubbard NE,Chapkin RS,Erickson KL

更新日期：1994-09-01 00:00:00

abstract：:Azaserine induced two phenotypically different populations of foci, namely, acidophilic and basophilic foci. The effects of dietary modification during the post-initiation phase of carcinogenesis were examined. A diet of 20% (w/w) unsaturated fat (unsat) compared to a 20% saturated fat (sat) diet or a control diet (5%...

journal_title：Lipids

authors： Roebuck BD

更新日期：1986-04-01 00:00:00

abstract：:After two months feeding either an (n-3) or an (n-6) fatty acid-rich diet, two groups of trout were switched to the (n-6) or the (n-3) fatty acid-rich diet, respectively. Half of each group was treated with 0.03% 5,8,11,14-eicosatetraynoic acid (ETYA) in the diet. Liver and intestinal brush border membrane lipids were...

journal_title：Lipids

authors： Pelletier X,Leray C

更新日期：1987-12-01 00:00:00

abstract：:Stearoyl-coenzyme A desaturase-1 (SCD1) is a rate-limiting enzyme that catalyzes the biosynthesis of monounsaturated fatty acids from saturated fatty acids. Recently, SCD1 down-regulation has been implicated in the prevention of obesity, and the improvement of insulin and leptin sensitivity. In this study, we examined...

journal_title：Lipids

authors： Beppu F,Hosokawa M,Yim MJ,Shinoda T,Miyashita K

更新日期：2013-05-01 00:00:00

abstract：:This study was undertaken to determine the effect of various lipid emulsions on the hepato-biliary system in rats. Rats were randomly divided into six groups and infused continuously for 48 hr with either long-chain triglycerides (LCT), medium-chain triglycerides (MCT) or a mixture of MCT and LCT. One group infused wi...

journal_title：Lipids

authors： Rubin M,Halpern Z,Livoff A,Wennberg A,Tietz A,Antebi E,Lichtenberg D

更新日期：1992-05-01 00:00:00

abstract：:A mutant of Mortierella alpina, JT-180, which is defective in its delta12 desaturase activity but exhibits enhanced activities of delta5 and delta6 desaturases, produced a high proportion (up to 80%) of odd-chain FA when grown on 3% n-heptadecane. Following growth of the mutant on n-heptadecane, three unusual odd-chai...

journal_title：Lipids

authors： Zhang S,Sakuradani E,Shimizu S

更新日期：2006-06-01 00:00:00

abstract：:Active lipases were isolated from the culture supernatant of the basidiomycetous fungus Pleurotus sapidus by foam fractionation. The pH value, the gas flow, and the foaming period were systematically varied to optimize the transport of the enzymes into the foam phase. On a 70-mL scale, a maximum recovery of hydrolytic...

journal_title：Lipids

authors： Linke D,Zorn H,Gerken B,Parlar H,Berger RG

更新日期：2005-03-01 00:00:00