Abstract:
:In an attempt to correlate the incorporation of fatty acids (FA) of different chain length into liver and biliary lipids, isolated rat livers were perfused for 2 h with Krebs-Ringer bicarbonate containing 1% albumin and 10 mumol of [1-14C]-labeled FA: C2, C8, C10, C12, C16, and C18:1. One to 1.36 mumol of medium-chain fatty acids (MCFA, C8, C10, and C12) and 6.6 mumol of long-chain FA (LCFA) were incorporated into liver lipids, 40% of the latter into phosphatidylcholine (PC). 14C-acetate (13 nmol) was incorporated into biliary cholesterol; 14C-MCFA contributed only 3.2-5 nmol; LCFA did not lead to newly synthesized cholesterol. Newly synthesized liver PC (2.75 to 3.25%) and newly synthesized liver cholesterol (6.5 to 10%) were secreted into bile. The specific radioactivity of biliary PC after infusion of all-saturated FA was 3.8-6.8 times higher than that of liver PC; for C18:1 it was only 1.7-fold. The specific radioactivity of biliary cholesterol, as compared to liver cholesterol, was 12 times higher for C2 and five times higher for MCFA. This indicates that a considerable proportion of the newly synthesized lipids was secreted into bile prior to significant mixing with preexisting liver PC and cholesterol pools. Liver PC contained 8% of unchanged 14C-C12; while 14C-C10 was not detected. Biliary PC, in contrast, contained 18% of unchanged 14C-C12 and 3% 14C-C10. These results suggest that after prolonged infusion of medium-chain triacylglycerols/long-chain triacylglycerols to patients, biliary PC may become enriched with MCTA. In addition, the oxidation of these FA may provide C-2 units which increase cholesterol synthesis.
journal_name
Lipidsjournal_title
Lipidsauthors
Rubin M,Pakula R,Gilat T,Tietz Adoi
10.1007/s11745-999-0400-xsubject
Has Abstractpub_date
1999-06-01 00:00:00pages
571-8issue
6eissn
0024-4201issn
1558-9307journal_volume
34pub_type
杂志文章相关文献
LIPIDS文献大全abstract::Apolipoprotein E (apo E) plays an essential role in lipoprotein metabolism, where it is involved in the clearance of chylomicrons and very low density lipoproteins. Apart from some rare variants, apo E exists in three common isoforms (E2, E3, and E4). The different isoforms have not only been associated with different...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-000-0500-7
更新日期:2000-01-01 00:00:00
abstract::This is the first report of stereospecific analyses of plant triglycerides isolated from seeds of distinct genotypes rather than from commerically refined oils. Triglycerides from six maize inbreds were analyzed. The strains exhibited a wide range of fatty acid compositions (palmitic acid 7.8-19.1%, oleic acid 17.0-43...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02531130
更新日期:1971-08-01 00:00:00
abstract::By in vivo and in vitro studies of L-(3-3H)serine and [9,10(n)-3H]palmitic acid incorporation into phospholipids, we show a change in the renewal of the ceramide moiety of sphingomyelin in the gills of euryhaline fish (sea bass and eels) when the animals were subjected to abrupt alterations in environmental salinity. ...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02522924
更新日期:1996-04-01 00:00:00
abstract::The effect of a saturated fat diet on the apoprotein composition and radioiodination properties of plasma very low density lipoprotein (VLDL) was studied in rats. After feeding the diet for 10 days, the proportion of 125I attached to VLDL lipid decreased from 50% (control animals) to 8%, the remainder (92%) being boun...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02533349
更新日期:1977-03-01 00:00:00
abstract::For three weeks, male Sprague-Dawley rats at either four weeks (young) or eight months (adult) of age were pair-fed one of the purified diets free of or containing either 0.2% of oxidized cholesterol mixture (cholesterol oxidation products) or 0.2% of cholesterol. Although the food intake was similar, dietary oxidized...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02536298
更新日期:1995-05-01 00:00:00
abstract::[1-14C]alpha-Linolenic acid was incubated with a particulate fraction of homogenate of leaves of the meadow buttercup (Ranunculus acris L.). The main product was a divinyl ether fatty acid, which was identified as 12-[1'(Z),3'(Z)-hexadienyloxy]-9(Z),11(E)-dodecadienoic acid. Addition of glutathione peroxidase and redu...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-998-0306-7
更新日期:1998-11-01 00:00:00
abstract::The suppression of plasma very low density lipoprotein (VLDL) triglyceride levels by dietary fish oils rich in polyunsaturated n-3 fatty acids has been attributed to decreased hepatic VLDL secretion. To investigate the effect of n-3 fatty acids on lipid metabolism and VLDL secretion in a tissue culture system, we incu...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02537160
更新日期:1990-09-01 00:00:00
abstract::During germination of seeds of the gymnosperm, Pinus pinea, radioactivity from [2-14C]-mevalonate proceeded principally through the anaerobic reactions leading to squalene in the first 24 hr in both the haploid endosperm and the diploid embryo, and only with succeeding time (3-9 days) in both cases was label transferr...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02533642
更新日期:1977-04-01 00:00:00
abstract::Neuronal membranes are highly enriched with docosahexaenoic acid (22:6n-3), and its content can be altered by ethanol consumption. We have previously reported that the 22:6n-3 status in membrane affects the biosynthesis of phosphatidylserine (PS), a phospholipid class which contains an exceptionally high proportion of...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02664769
更新日期:2000-02-01 00:00:00
abstract::The sn-position of FA in membrane lipids has an influence on the physiological function of cells, is predictive for diseases, and therefore is useful for diagnostics. The current study compares the compositions of acyl chain substituents in the sn-1 and sn-2 positions of the glycerol backbones of phospholipids derived...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-005-1377-1
更新日期:2005-02-01 00:00:00
abstract::Phytophthora species are eukaryotic sterol auxotrophs that possess the ability to grow, albeit poorly, in the complete absence of sterols. Growth of Phytophthora is often improved substantially when an exogenous source of sterol is provided. Additionally, sterols may be required for sexual and asexual sporulation in P...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-000-0519-9
更新日期:2000-03-01 00:00:00
abstract::Incorporation of [14C] from acetoacetate, D(-)- and L(+)-3-hydroxybutyrate, glucose, glutamine, acetate and palmitate in cellular lipids were studied in cultures in human diploid fibroblasts (HDF). The results showed that acetoacetate was 2-10 times more effective as a lipogenic precursor than was either D- or L-3-hyd...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02535063
更新日期:1981-09-01 00:00:00
abstract::We evaluated the effects of dietary lipid class (phospholipid vs. neutral lipid) and level of n-3 long-chain PUFA (LC-PUFA) on the growth, digestive enzymatic activity, and histological organization of the intestine and liver in European sea bass larvae. Fish were fed from the onset of exogenous feeding at 7 to 37 d p...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-005-1422-0
更新日期:2005-06-01 00:00:00
abstract::CLA has a range of biological properties, including effects on lipid metabolism and body composition in experimental animals. The prevalent isomer of CLA found in the human diet is 9c,11 t-CLA, and it is predominantly found in products containing fat from ruminant animals. This study investigated the effect of dietary...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-002-0950-y
更新日期:2002-07-01 00:00:00
abstract::Cis-9-conjugated, trans-11-conjugated linoleic acid (CLA) is known for its positive activities on human health. The synthesis of cis-9, trans-11 CLA in mammary glands is generally thought to be catalyzed by stearoyl-CoA desaturase 1 (SCD1), but this has not been rigorously established. In this study, we hypothesized t...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1002/lipd.12077
更新日期:2018-06-01 00:00:00
abstract::Although migrating birds use stored triacylglycerol as their primary fuel for flight, they must retain sufficient stores of omega 6 and omega 3 fatty acids to sustain reproduction after the spring migration. Hepatic monoacylglycerol:acyl-coenzyme A acyltransferase (EC 2.3.1.22) (MGAT) activity is associated with physi...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02536701
更新日期:1994-11-01 00:00:00
abstract::The composition of the glycosphingolipids of the human gastrointestinal tract was studied. The major neutral glycosphingolipids were ceramide monohexosides (e.g., GalCer, GlcCer), LacCer, Gb3Cer, Gb4Cer and more polar ones with more than four sugars, whereas neither Gg3Cer nor Gg4Cer were present. The acidic glycosphi...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02535996
更新日期:1993-08-01 00:00:00
abstract::A protocol, based on the use of Pseudomonas lipase, is presented to measure quantitatively the amount of triacylglycerols in extracts from cultured cells of tissues. Since the lipase also acts on di- and monoacylglycerols, separation of the extracts by thin-layer chromatography is recommended. In order to allow the li...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-006-0166-1
更新日期:1997-12-01 00:00:00
abstract::With the passage of the U.S. Dietary Supplement Health and Education Act of 1994, dehydroepiandrosterone (DHEA, 5-androsten-3beta-ol-17-one) has become widely available, and a large and growing market has developed for this "fountain of youth." DHEA has been shown to have significant beneficial effects in animals, whi...
journal_title:Lipids
pub_type: 杂志文章,评审
doi:10.1007/s11745-000-0529-7
更新日期:2000-03-01 00:00:00
abstract::Red blood cell (RBC) fatty acid (FA) patterns have been shown to predict risk for cardiovascular and other chronic diseases. As part of a project analyzing RBC samples from the Women's Health Initiative Memory Study (WHIMS) we observed implausibly low levels of highly unsaturated fatty acids (HUFA) suggestive of degra...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-012-3693-y
更新日期:2012-09-01 00:00:00
abstract::The enzyme(s) present in acetonedried powder of rat and rabbit aortas, which catalyzes the synthesis and hydrolysis of cholesteryl ester, was purified partially by acid precipitation, acetone fractionation, O-(diethylaminoethyl) cellulose chromatography, and Sephadex G-100 filtration. The synthetic activity was purifi...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02532453
更新日期:1975-06-01 00:00:00
abstract::Mevalonic acid-derived intermediates in the cholesterol biosynthetic pathway have been recognized as being critical to the isoprenylation of a variety of growth-regulating proteins, including those of the RAS superfamily. Treatment of cells with lovastatin, a hydroxymethylglutaryl coenzyme A (HMG-CoA) reductase inhibi...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-002-0879-1
更新日期:2002-02-01 00:00:00
abstract::Polar lipids of bacteria from the class Thermomicrobia are known to contain long-chain 1,2-diols instead of glycerol, although the nature of polar head groups has not been investigated. We have studied phospholipid classes of two species from the class Thermomicrobia-Sphaerobacter thermophilus and Thermomicrobia sp. W...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-014-3979-3
更新日期:2015-03-01 00:00:00
abstract::The distribution of dolichol in rat liver was studied. Upon high-speed centrifugation, 9% of the total tissue dolichol was recovered in the supernatant. Dolichol was enclosed in vesicles and in lipidic particles which were isolated by gel filtration and density gradient centrifugation. The particles had a diameter of ...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02537050
更新日期:1992-01-01 00:00:00
abstract::Glucose-sensitive liposomes were prepared by incorporating hydrophobically modified glucose oxidase (EC 1.1.3.4.) into the liposomal bilayer of dioleoylphosphatidylethanolamine and cholesteryl hemisuccinate. For the release test, calcein, a fluorescence marker, was entrapped in the liposomes. The liposomes were stable...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-008-3223-0
更新日期:2008-10-01 00:00:00
abstract::Fatty acid alpha-hydroxylase from Sphingomonas paucimobilis is an unusual cytochrome P450 enzyme that hydroxylates the alpha-carbon of fatty acids in the presence of H2O2. Herein, we describe our investigation concerning the utilization of various substrates and the optical configuration of the alpha-hydroxyl product ...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-000-533-y
更新日期:2000-04-01 00:00:00
abstract::Rats were fed semipurified diets containing olive oil or partially hydrogenated corn oil at the 5 or 20% level for ca. 30 days. These fat diets contained the same amount of octadecenoate but differed in the geometry with respect to each fat level. Contents of t-18:1 were 26% and 41% of total fatty acids, respectively....
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02534500
更新日期:1984-02-01 00:00:00
abstract::Saccharomyces cerevisiae cells were demonstrated to contain lysophosphatidylcholine (lysoPtdCho) acyltransferase (E.C. 2.3.1.23) activity. The enzyme displayed Km(app) of 69 microM for lysoPtdCho and 152 microM for oleoyl CoA. Enzyme activity was not affected by the addition of 1 mM Mg2+, Mn2+, Ca2+, or 200 mM EDTA. H...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-998-0328-1
更新日期:1998-12-01 00:00:00
abstract::In this study, to obtain further information on the structural diversity of non-methylene-interrupted (NMI) fatty acids (FA) in the gonads of the limpet Cellana toreuma, previously unreported minor NMI FA, fractionated by argentation thin-layer chromatography (TLC), in the ovaries were characterized by gas chromatogra...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1002/lipd.12225
更新日期:2020-05-01 00:00:00
abstract::The aim of this study was to determine the influence of long-term docosahexaenoic acid (DHA) dietary supplementation on the erythrocyte fatty acid profile and oxidative balance in soccer players after training and acute exercise. Fifteen volunteer male athletes (age 20.0 ± 0.5 years) were randomly assigned to a placeb...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-014-3976-6
更新日期:2015-02-01 00:00:00