Abstract:
:The aim of this study was to develop a simple method for simultaneous determination of selected cis/cis PUFA-LNA (18:2), ALA (18:3), GLA (18:3), EPA (20:5), and DHA (22:6) by silver ion high-performance liquid chromatography coupled to a diode array detector (Ag-HPLC-DAD). The separation was performed on three Luna SCX Silver Loaded columns connected in series maintained at 10 °C with isocratic elution by 1% acetonitrile in n-hexane. The applied chromatographic system allowed a baseline separation of standard mixture of n-3 and n-6 fatty acid methyl esters containing LNA, DHA, and EPA and partial separation of ALA and GLA positional isomers. The method was validated by means of linearity, precision, stability, and recovery. Limits of detection (LOD) for considered PUFA standard solutions ranged from 0.27 to 0.43 mg L(-1). The developed method was used to evaluate of n-3 and n-6 fatty acids contents in plant and fish softgel oil capsules, results were compared with reference GC-FID based method.
journal_name
Lipidsjournal_title
Lipidsauthors
Czajkowska-Mysłek A,Siekierko U,Gajewska Mdoi
10.1007/s11745-016-4133-1subject
Has Abstractpub_date
2016-04-01 00:00:00pages
413-21issue
4eissn
0024-4201issn
1558-9307pii
10.1007/s11745-016-4133-1journal_volume
51pub_type
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