Abstract:
:A simple and sensitive method to analyze mixtures of desmosterol, 7-dehydrocholesterol and cholesterol is described. The method involves the oxidative conversion of the sterols with cholesterol oxidase, followed by high performance liquid chromatographic (HPLC) analysis. A C18 reversed phase column (3 microns, 75 X 4.6 mm) and a mixture of methanol and acetonitrile (1:1, v/v) at a rate of 1 ml/min are used to separate the sterols. The eluted sterols are quantified by measuring UV absorption at 240 nm. As little as 10 pmoles of sterol can be quantified under these conditions.
journal_name
Lipidsjournal_title
Lipidsauthors
Goh EH,Colles SM,Otte KDdoi
10.1007/BF02535083subject
Has Abstractpub_date
1989-07-01 00:00:00pages
652-5issue
7eissn
0024-4201issn
1558-9307journal_volume
24pub_type
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