Analysis of conjugated bile acids by high performance liquid chromatography and mass spectrometry.

Abstract:

:Because of the known advantages of coupling high performance liquid chromatography with mass spectrometry (HPLC-MS) in biological fluids, studies on the reversed-phase HPLC-MS system for direct analysis of conjugated bile acids in human bile samples are described. Ten samples of gallbladder bile of apparently healthy subjects were examined. The amounts of each tauro- and glycoconjugated bile acid as trifluoracetate were determined by mass fragmentography. Quantitation of at least 1 ng of each bile acid was possible.

journal_name

Lipids

journal_title

Lipids

authors

Mingrone G,Greco AV,Boniforti L,Passi S

doi

10.1007/BF02534697

subject

Has Abstract

pub_date

1983-01-01 00:00:00

pages

90-5

issue

1

eissn

0024-4201

issn

1558-9307

journal_volume

18

pub_type

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