Abstract:
:The lipid quality of ruminant products is largely determined by the extent of rumen microbial biohydrogenation (BH) of polyunsaturated fatty acids (FAs) and the substances formed thereby. In vitro batch incubations with mixed rumen bacteria were tracked over 24 h to characterize the profiles and kinetics of the BH products from three lipid sources: pure linoleic acid (c9,c12-18:2), pure linolenic acid (c9,c12,c15-18:3) and linseed oil (mainly c9,c12,c15-18:3 in triacylglycerols). After 24 h of incubation biohydrogenation was more complete for c9,c12-18:2, which gave mainly stearic acid (18:0), than for c9,c12,c15-18:3, which yielded mainly trans-18:1 FAs. This suggests inhibition of the final BH step (18:1 to 18:0). Incubations of c9,c12-18:2 resulted in high levels of carbon 10- and 12-desaturated 18:1, t10,c12- and c9,t11-CLAs. Incubations of c9,c12,c15-18:3 resulted in high levels of t11-18:1, carbon 13- and 15-desaturated 18:1 as well as t11,c15-18:2 and 11,13-CLAs. A comparative study of linolenic acid and linseed oil kinetics revealed that the BH process was not significantly slowed by the esterification of polyunsaturated FAs, but may have been limited by the isomerization step in which the cis12 double bond goes to the trans11 position. The disappearance rates of c9,c12-18:2 and c9,c12,c15-18:3 ranged from 23.6 to 44.6%/h. The wide variety of BH intermediates found here underlines the large number of possible BH pathways. These data help provide a basis for dynamic approaches to BH processes.
journal_name
Lipidsjournal_title
Lipidsauthors
Jouany JP,Lassalas B,Doreau M,Glasser Fdoi
10.1007/s11745-007-3032-xsubject
Has Abstractpub_date
2007-04-01 00:00:00pages
351-60issue
4eissn
0024-4201issn
1558-9307journal_volume
42pub_type
杂志文章相关文献
LIPIDS文献大全abstract::Subcellular membranes were analyzed for their lipid composition and protein content at two developmental points representing the third instar wandering larvae and prepupal stages of Drosophila. At both stages, phosphatidylethanolamine (PE) and phosphatidylcholine (PC) were the major constituents with phosphatidylinosi...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02535576
更新日期:1992-12-01 00:00:00
abstract::The effects of increasing concentrations of eicosapentaenoic acid (20:5n-3; EPA) and oleic acid (18:1n-9; OA) on esterification to triacylglycerols (TG) and phospholipids (PL), and the relationship to formation and secretion of the very low density lipoproteins (VLDL) were compared in the isolated perfused rat liver. ...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02535940
更新日期:1993-05-01 00:00:00
abstract::An important enzyme involved in phospholipid turnover is the acyl-CoA: lysophosphatidylcholine acyltransferase (LPCAT). Here, we report characterization of a newly discovered human LPCAT (LPCAT3), which has distinct substrate preferences strikingly consistent with a role in phosphatidylcholine (PtdCho) remodeling and ...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-008-3233-y
更新日期:2008-10-01 00:00:00
abstract::Bile acid contents were determined for isolated rat hepatocytes. During the course of isolating the hepatocytes, perfusion of rat liver with buffer containing 2% albumin eluted a significant amount of bile acids. The elution was proportional to the volume of the buffer and attributable to albumin in the buffer. The is...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02535094
更新日期:1982-03-01 00:00:00
abstract::We investigated the effect of oral supplementation with evening primrose oil, containing 72% linoleic acid (18:2n-6) and 10% gamma-linolenic acid (18:3n-6), on the epidermal and neutrophil phospholipid fatty acid composition in 15 patients with atopic dermatitis (AD). Three different dose levels, 4, 8 and 12 capsules ...
journal_title:Lipids
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1007/BF02536604
更新日期:1991-07-01 00:00:00
abstract::A method was developed to analyze and quantify volatile fatty acids (VFA) such as acetic, propionic, butyric, isobutyric, valeric and isovaleric acids in biological specimens. To obtain good sample transfer into the chromatographic system an organic solvent had to be used together with an aqueous milieu, thus improvin...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02537302
更新日期:1987-03-01 00:00:00
abstract::This study compared (i) the relative effects of long-chain triglycerides (LCT) and medium-chain triglycerides (MCT), (ii) the influence of amount of MCT, and (iii) the impact of medium-chain fatty acid position, on plasma and lymphatic triglycerides and portal vein free fatty acids. The animals were fed approximately ...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-998-0204-z
更新日期:1998-03-01 00:00:00
abstract::A group of Angus beef cattle was removed from temperate pastures and fed a very low beta-carotene cereal-based ration in a feedlot for over 300 d. Half the group was supplemented weekly with retinyl palmitate (at the rate of 60,000 IU vitamin A/100 live weight (LW)/day), sufficient to offset clinical vitamin A deficie...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-006-5107-5
更新日期:2006-04-01 00:00:00
abstract::The pulp from unerupted calves' teeth was found to contain low levels of glycerol ester hydrolase activity. Solutions in Tris buffer cleared tributyrylglycerol agar and hydrolyzed emulsified olive oil. The average quantity of free fatty acids released in five assays with olive oil was: 2.8, S.D.+/-1.4. Specific activi...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02533302
更新日期:1977-02-01 00:00:00
abstract::Both diacyl GPC (glycerylphosphorylcholine) and diacyl GPE (glycerylphosphorylethanolamine) are synthesized in human placental tissue from their respective monoacyl precursors. The origin of the monacyl phosphatides is apparently not the result of placental phosphatide acyl-hydrolase activity. The most likely source i...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02531854
更新日期:1967-09-01 00:00:00
abstract::In vivo intestinal perfusion was used to follow the absorption of three different choline glycerophospholipids (CGP) in guinea pig. These included 1-[3H]palmitoyl-2-acyl-sn-glycero-3-phosphocholine (diacyl-GPC), 1-[3H]-O-hexadecyl-2-acyl-sn-glycero-3-phosphocholine (alkylacyl-GPC) and 1,2-di-O-hexadecyl-sn-glycero-3-p...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02534872
更新日期:1987-01-01 00:00:00
abstract::Gallstone formation and dissolution were studied in a prairie dog model of cholesterol (CH) cholelithiasis. Gallstones were induced in 49 prairie dogs by feeding 1.2% CH in a nutritionally adequate semisynthetic diet for 6 wk (period 1). At 6 wk, gallstones had developed in all animals examined. The diets were modifie...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02534055
更新日期:1986-09-01 00:00:00
abstract::cis-5,cis-9-Octadecadienoic acid (2.7%) andcis-5,cis-9,cis-12-octadecatrienoic acid (24.9%) are present in the seed oil ofLarix leptolepis. The double bond positions were identified by ozonolysis and by gas chromatography-mass spectrometry of methoxy derivatives. Small quantities of branched chain acids of various cha...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02532446
更新日期:1975-07-01 00:00:00
abstract::Peanut oil was shown to be atherogenic in cholesterol-fed rats, rabbits, and monkeys. However, after randomization, a process in which the fatty acids in peanut oil are randomly rearranged, its atherogenicity was significantly reduced in cholesterol-fed rabbits and monkeys. The mechanism for this effect remains unknow...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-999-0482-5
更新日期:1999-12-01 00:00:00
abstract::Flaxseed, echium, and canola oils contain alpha-linolenic acid (18:3n-3, ALA) in a range of concentrations. To examine their effect on elevating cardiac levels of long-chain n-3 FA, diets based on these n-3-containing vegetable oils were fed to rats for 4 wk. Sunflower oil, which contains little ALA, was a comparator....
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-005-1461-6
更新日期:2005-10-01 00:00:00
abstract::The weight loss supplement conjugated linoleic acid (CLA) consists of an equal mixture of trans-10,cis-12 (10,12) and cis-9,trans-11 (9,11) isomers. However, high levels of mixed CLA isomers, or the 10,12 isomer, causes chronic inflammation, lipodystrophy, or insulin resistance. We previously demonstrated that 10,12 C...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-012-3711-0
更新日期:2012-11-01 00:00:00
abstract::The suppression of plasma very low density lipoprotein (VLDL) triglyceride levels by dietary fish oils rich in polyunsaturated n-3 fatty acids has been attributed to decreased hepatic VLDL secretion. To investigate the effect of n-3 fatty acids on lipid metabolism and VLDL secretion in a tissue culture system, we incu...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02537160
更新日期:1990-09-01 00:00:00
abstract::This work reports the effect of the apolipoproteins A-I and A-II (apoA-I and apoA-II) on the release of tumor necrosis factor (TNF)-alpha, interleukin (IL)-1beta, IL-8, and IL-1 receptor antagonist (IL-1Ra) and on the oxidative burst of human neutrophils. By themselves, apoA-I and apoA-II do not affect the basal liber...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-002-0981-4
更新日期:2002-09-01 00:00:00
abstract::Fatty acid composition of plasma phospholipids (PL), triglycerides (TG), and sterol esters (STE) was determined by high-resolution capillary gas-liquid chromatography in 22 obese children (age: 13.7 +/- 1.4 y, body weight relative to normal weight for height: 170 +/- 24%, mean +/- SD) and compared with data obtained i...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02529877
更新日期:1996-03-01 00:00:00
abstract::FA ethyl esters (FAEE) are nonoxidative metabolites of ethanol produced by the esterification of FA and ethanol. FAEE have been implicated as mediators of ethanol-induced organ damage in vivo and in vitro, and are markers of ethanol intake. Upon ethanol intake, FAEE are synthesized in the liver and pancreas in signifi...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-006-1160-3
更新日期:2003-10-01 00:00:00
abstract::Apolipoprotein E (apo E) plays an essential role in lipoprotein metabolism, where it is involved in the clearance of chylomicrons and very low density lipoproteins. Apart from some rare variants, apo E exists in three common isoforms (E2, E3, and E4). The different isoforms have not only been associated with different...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-000-0500-7
更新日期:2000-01-01 00:00:00
abstract::According to the model of "response to injury," the arterial endothelium is occasionally injured in hyperlipidemia, hypertension, diabetes mellitus and in other states known as risk factors. The ensuing inflammatory response is modulated by cytokines and growth factors, among them platelet-derived growth factor (PDGF)...
journal_title:Lipids
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1007/s11745-001-0689-5
更新日期:2001-01-01 00:00:00
abstract::The concentrations of the major neutral lipid and phospholipid classes in the plasma of rats bearing hepatoma 7288CTC were determined at various times after transplantation. The fatty acid composition of each lipid class was also analyzed quantitatively as tumor growth progressed. Generally, most lipid classes exhibit...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02534066
更新日期:1980-06-01 00:00:00
abstract::The effect of SM-10661, a selective antagonist of platelet-activating factor (PAF), on passive anaphylactic bronchoconstriction was examined in guinea pigs. A challenge of ovalbumin to passively sensitized guinea pigs induced bronchoconstriction, which peaked at 4 min. When SM-10661 was administered intravenously 2 mi...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02536553
更新日期:1991-12-01 00:00:00
abstract::The effect of Rauscher Leukemia Virus (MuLV-R) infection on the lipid composition of mouse spleen from BALB/c mice was investigated. Drastic changes in the lipid composition of the spleen as a result of tumor growth induced by the virus could be demonstrated at 21 days after infection. The molar ratio of cholesterol t...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02533406
更新日期:1976-11-01 00:00:00
abstract::The aim of the present study was to identify the effects of dehydroepiandrosterone (DHEA) on hepatic lipid metabolism parameters and lipogenic gene mRNA expression in broiler chickens. A total of 72 1-day-old broiler chicks received a common basal diet with DHEA added at either 0 (control), 5 or 20 mg/kg feed. In the ...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-007-3104-y
更新日期:2007-11-01 00:00:00
abstract::The hypolipidemic drug clofibrate is known to affect the hepatic transport of various organic anions including bilirubin, fatty acids and sulfobromophthalein. Changes in the rate of metabolism and/or intracellular transport have been claimed responsible for the effect. To evaluate these possibilities, the transport of...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02535153
更新日期:1989-05-01 00:00:00
abstract::In the present paper, problems in connection with assay of the activity of magnesium-dependent rat liver phosphatidate phosphohydrolase (PAP) are discussed. PAP activity is usually measured by following the production of diacylglycerol or inorganic phosphate from the substrate phosphatidate. These two methods may give...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02522468
更新日期:1996-10-01 00:00:00
abstract::The effects of n-3 fatty acids on plasma lipids, lipoproteins and apoproteins have usually been studied in humans after feeding of purified fish oil. This study describes the effect of a natural diet, containing salmon as the source of n-3 fatty acids, on these parameters as compared to a diet very low in n-3 fatty ac...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02544001
更新日期:1991-02-01 00:00:00
abstract::The schweinfurthins have potent antiproliferative activity in multiple glioblastoma multiforme (GBM) cell lines; however, the mechanism by which growth is impeded is not fully understood. Previously, we demonstrated that the schweinfurthins reduce the level of key isoprenoid intermediates in the cholesterol biosynthet...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-015-4083-z
更新日期:2015-12-01 00:00:00