Abstract:
:Cholesterol and cholesteryl esters were separated according to their carbon number and number of double bonds by high performance reversed-phase chromatography (HPRC) using acetonitrile/chloroform/methanol (1:1:1, v/v) as a mobile phase. It was found that within the same equivalent carbon number (ECN) category, cholesteryl esters with the highest number of double bonds eluted ahead of those with a lower number of double bonds, and with the cis isomers eluting ahead of their trans partners. Thus, cholesteryl oleate (C27-18:1c) elutes ahead of cholesteryl palmitate (C27-16:0) and ahead of cholesteryl elaidate (C27-18:1t). Human lipoprotein, as well as rat liver cholesteryl esters, were separated using this technique.
journal_name
Lipidsjournal_title
Lipidsauthors
Perkins EG,Hendren DJ,Bauer JE,El-Hamdy AHdoi
10.1007/BF02534908subject
Has Abstractpub_date
1981-08-01 00:00:00pages
609-13issue
8eissn
0024-4201issn
1558-9307journal_volume
16pub_type
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