Abstract:
:A fluorescent radiolabeled triacylglycerol has been synthesized by using a fluorescent fatty acid (pyrene decanoic acid) and a radiolabeled oleic acid. This analog of the natural substrate, 1(3)pyrene decanoic-2,3 (1,2)-dioleoyl-sn-glycerol, has been tested as substrate for determining lipoprotein lipase and hepatic triacylglycerol lipase activities in post-heparin plasma. Optimal conditions for the determination of the two post-heparin plasma lipases were similar to those using radiolabeled triolein. Using this substrate, both post-heparin lipases exhibited their characteristic properties (pH optimum and effect of inhibitors) and attacked external ester bonds (1 or 3) containing pyrene decanoic and oleic acids at a similar rate.
journal_name
Lipidsjournal_title
Lipidsauthors
Dousset N,Negre A,Salvayre R,Rogalle P,Dang QQ,Douste-Blazy Ldoi
10.1007/BF02535605subject
Has Abstractpub_date
1988-06-01 00:00:00pages
605-8issue
6eissn
0024-4201issn
1558-9307journal_volume
23pub_type
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