Use of a fluorescent radiolabeled triacylglycerol as a substrate for lipoprotein lipase and hepatic triglyceride lipase.

Abstract:

:A fluorescent radiolabeled triacylglycerol has been synthesized by using a fluorescent fatty acid (pyrene decanoic acid) and a radiolabeled oleic acid. This analog of the natural substrate, 1(3)pyrene decanoic-2,3 (1,2)-dioleoyl-sn-glycerol, has been tested as substrate for determining lipoprotein lipase and hepatic triacylglycerol lipase activities in post-heparin plasma. Optimal conditions for the determination of the two post-heparin plasma lipases were similar to those using radiolabeled triolein. Using this substrate, both post-heparin lipases exhibited their characteristic properties (pH optimum and effect of inhibitors) and attacked external ester bonds (1 or 3) containing pyrene decanoic and oleic acids at a similar rate.

journal_name

Lipids

journal_title

Lipids

authors

Dousset N,Negre A,Salvayre R,Rogalle P,Dang QQ,Douste-Blazy L

doi

10.1007/BF02535605

subject

Has Abstract

pub_date

1988-06-01 00:00:00

pages

605-8

issue

6

eissn

0024-4201

issn

1558-9307

journal_volume

23

pub_type

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