Abstract:
:CDPcholine:1,2-diacylglycerol cholinephosphotransferase (EC 2.7.8.2) is located on the cytoplasmic side of the endoplasmic reticulum, and catalyzes the final step in the synthesis of phosphatidylcholine via the CDPcholine pathway. The enzyme was solubilized from hamster liver microsomes by 3% Triton QS-15, and partially purified by DEAE-Sepharose chromatography and Sepharose 6B chromatography. The microsomal and partially purified enzymes displayed similar pH profile, and both showed absolute requirement for Mg++ or other divalent cations. The Km values of CDPcholine were similar between microsomal and partially purified enzyme, whereas the Km value for diacylglycerol was substantially lowered when the enzyme was partially purified. Hamster heart cholinephosphotransferase was not solubilized by Triton QS-15.
journal_name
Lipidsjournal_title
Lipidsauthors
O KM,Choy PCdoi
10.1007/BF02562217subject
Has Abstractpub_date
1990-02-01 00:00:00pages
122-4issue
2eissn
0024-4201issn
1558-9307journal_volume
25pub_type
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