Abstract:
:Fecal bile acid and neutral sterol patterns were studied in eight healthy adult volunteers who were challenged with Vibrio cholerae classical Ogawa 395 strain in the course of vaccine development studies. Bacterial 7 alpha-dehydroxylation of cholic and chenodeoxycholic acids was not altered during experimentally induced cholera diarrhea, despite the fact that fecal weight in g/day (wet wt) was increased greatly during diarrhea (1913 +/- 390 vs 161 +/- 11 in controls, p less than 0.005). Consistent with the findings on bile acids, no significant changes in the production of coprostanol, epicoprostanol, or coprostanone were observed although the percentage of unmodified cholesterol was increased during the diarrheal episode (20.7 +/- 3.3% vs 11.9 +/- 2.3, p less than 0.02). Total concentrations of both bile acids and cholesterol in mg/g of feces (wet wt) were decreased considerably as a result of diarrhea). However, total bile acid and neutral steroid excretions in mg/kg/day in subjects with and without diarrhea do not appear to be different. Intestinal transit times, measured in eight subjects by the use of carmine red dye, were found to be shortened in diarrhea (5.8 +/- 1.1 hr vs 23.4 +/- 4.1 hr in controls, p less than 0.001). The results from this study are similar to those observed in experimentally induced travellers' diarrhea associated with toxigenic Escherichia coli, but they are in striking contrast to the changes in gastrointestinal steroid metabolism observed in acute shigellosis, an invasive intestinal infection.
journal_name
Lipidsjournal_title
Lipidsauthors
Huang CT,Levine MM,Daoud GS,Nalin DR,Nichols BLdoi
10.1007/BF02535367subject
Has Abstractpub_date
1982-09-01 00:00:00pages
612-6issue
9eissn
0024-4201issn
1558-9307journal_volume
17pub_type
杂志文章相关文献
LIPIDS文献大全abstract::Facile procedures are described for nearly quantitative conversion of saturated and unsaturated fatty aldehydes to their dimethyl acetals, and vice versa, at low temperatures. The methods are based on the chemical behavior of aldehydes and dimethyl acetals in 100% sulfuric acid. Under the experimental conditions descr...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02532679
更新日期:1966-09-01 00:00:00
abstract::The anesthetic potency of four derivatives of n-octane was measured by tadpole righting reflex and expressed as effective millimolar concentration of drug in membrane, EDM50. Potency diminished (ED50 increased) in this order: 1-octanol, EDM50 = 5.5; 1-(2-methoxyethoxy)octane, EDM50 = 28; 1-methoxyoctane, EDM50 = 61; a...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02534936
更新日期:1986-06-01 00:00:00
abstract::Starting at 21 days of age, groups of six rats each were fed a basal Torula yeast diet supplemented with 0.4% L-methionine and varying amounts of vitamin E as dl-alpha tocopherol acetate, selenium as sodium selenite, and with either 10% stripped corn oil, stripped lard, or coconut oil. By 7 wk, pentane production by r...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02533708
更新日期:1978-06-01 00:00:00
abstract::The naturally occurring (6Z)-(±)-2-methoxy-6-hexadecenoic acid (1) and (6Z)-(±)-2-methoxy-6-octadecenoic acid (2) were synthesized in 7-8 steps with 38 and 13% overall yields, respectively, by using an acetylide coupling approach, which made it possible to obtain a 100% cis-stereochemistry for the double bonds. In a s...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-017-4262-1
更新日期:2017-06-01 00:00:00
abstract::A method was developed to analyze and quantify volatile fatty acids (VFA) such as acetic, propionic, butyric, isobutyric, valeric and isovaleric acids in biological specimens. To obtain good sample transfer into the chromatographic system an organic solvent had to be used together with an aqueous milieu, thus improvin...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02537302
更新日期:1987-03-01 00:00:00
abstract::Conjugated linoleic acid (CLA) has been demonstrated to reduce body fat in animals. However, the mechanism by which this reduction occurs is unknown. Leptin may mediate the effect of CLA to decrease body fat. We assessed the effects of 64 d of CLA supplementation (3 g/d) on circulating leptin, insulin, glucose, and la...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-000-0586-y
更新日期:2000-07-01 00:00:00
abstract::Lipid metabolism of male Fisher 344 rats aged 2-24 months was studied. Serum and liver cholesterol levels did not display the age-related gradual increase seen in other rat strains. An increase in the serum plus liver cholesterol pool from 2 to 6 months was followed by a plateau through 18 months and then another incr...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02532876
更新日期:1976-08-01 00:00:00
abstract::The molecular species in mixtures of phospholipid hydroperoxides are difficult to separate and identify by typical chromatographic and mass spectrometric techniques. As reported by Havrilla and coworkers, silver ion coordination ion-spray mass spectrometry (CIS-MS) has proven to be a powerful technique for the identif...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-001-0841-2
更新日期:2001-11-01 00:00:00
abstract::This study investigated a possible role for the epidermal growth factor (EGF) receptor protein tyrosine kinase in phosphoinositide metabolism with plasma membrane vesicles from human epidermoid carcinoma (A431) cells. We found a novel chloroform-soluble product radiolabeled with [gamma-32P]ATP that did not migrate fro...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02534242
更新日期:1985-07-01 00:00:00
abstract::Polar lipids of bacteria from the class Thermomicrobia are known to contain long-chain 1,2-diols instead of glycerol, although the nature of polar head groups has not been investigated. We have studied phospholipid classes of two species from the class Thermomicrobia-Sphaerobacter thermophilus and Thermomicrobia sp. W...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-014-3979-3
更新日期:2015-03-01 00:00:00
abstract::To address the role of cell membrane neutral sphingomyelinase (EC 3.1.4.12; SMase) in the regulation of cholesterol metabolism in the liver parenchymal cell, we examined the effect of exogenous neutral SMase on the metabolism of cholesteryl esters and the secretion of VLDL and biliary lipids in isolated rat hepatocyte...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-003-1031-y
更新日期:2003-01-01 00:00:00
abstract::During our ongoing project on the biosynthesis of R-(+)-octane-1,3-diol the metabolism of linoleic acid was investigated in stored apples after injection of [1-14C]-, [9,10,12,13-3H]-,13C18- and unlabeled substrates. After different incubation periods the products were analyzed by gas chromatography-mass spectroscopy ...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-999-0375-7
更新日期:1999-04-01 00:00:00
abstract::Decreased triacylglycerol synthesis within hepatocytes due to decreased diacylglycerol acyltransferase (DGAT) activity has been suggested to be an important mechanism by which diets rich in fish oil lower plasma triacylglycerol levels. New findings suggest that eicosapentaenoic acid (EPA), and not docosahexaenoic acid...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-999-0445-x
更新日期:1999-09-01 00:00:00
abstract::This paper represents the first synthesis, spectroscopic characterization, and antitumor evaluation of F-, N-, and S-containing C4alpha-FA derivatives of podophyllotoxin. In a synthetic strategy, a FA unit of 4-O-podophyllotoxinyl 12-hydroxyoctadec-Z-9-enoate 2, a derivative of podophyllotoxin, was functionalized at t...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-006-1397-x
更新日期:2005-04-01 00:00:00
abstract::A one-carbon degradation of long-chain fatty acids, which was found to occur in the brains of rats in vivo, has been investigated in a brain microsomal fraction in vitro. Decarboxylation of the alpha-hydroxy acid, a possible intermediate product between the substrate and the next shorter acid, in the presence of brain...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02531201
更新日期:1968-05-01 00:00:00
abstract::Growth hormone (GH) has many metabolic effects, but its mechanism(s) of action are not fully understood. We studied the short-term effects of endogenously produced GH on liver delta 6-desaturase activity and adipose and liver lipid fraction fatty acid composition in transgenic mice. MG101 transgenic mice ages 73-114 d...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02522612
更新日期:1996-02-01 00:00:00
abstract::Hepatocytes isolated from rainbow trout fed on diets containing either fish oil or olive oil were incubated with individual odd-chain length unsaturated fatty acids (19:1n-9, 19:2n-6, 19:3n-3, 21:2n-6, 21:3n-6, 21:4n-6, 21:3n-3, and 21:5n-3) to examine whether these fatty acids were substrates for modification by desa...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-997-0078-0
更新日期:1997-06-01 00:00:00
abstract::Phytophthora species are eukaryotic sterol auxotrophs that possess the ability to grow, albeit poorly, in the complete absence of sterols. Growth of Phytophthora is often improved substantially when an exogenous source of sterol is provided. Additionally, sterols may be required for sexual and asexual sporulation in P...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-000-0519-9
更新日期:2000-03-01 00:00:00
abstract::Developing rat brain has the capacity for either delta 9 or delta 6 desaturation of fatty acids. In liver, evidence supports the existence of separate enzymes for each reaction, but it is not known whether in brain delta 9 or delta 6 desaturation of saturated fatty acids involves distinct enzymes. We have used fatty a...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02533513
更新日期:1979-09-01 00:00:00
abstract::Accurate quantification of the major classes and subfractions of human serum lipoproteins is an important analytical need in the characterization and evaluation of therapy of lipid and lipoprotein abnormalities. For calibrating the analytic ultracentrifuge (AnUC), we routinely use a Beckman calibration wedge cell with...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02534491
更新日期:1984-07-01 00:00:00
abstract::The physiological role of long-chain fatty acyl-CoA is thought to be primarily in intermediary metabolism of fatty acids. However, recent data show that nM to microM levels of these lipophilic molecules are potent regulators of cell functions in vitro. Although long-chain fatty acyl-CoA are present at several hundred ...
journal_title:Lipids
pub_type: 杂志文章,评审
doi:10.1007/BF02522684
更新日期:1996-09-01 00:00:00
abstract::Chemical composition of subcellular components of HeLa, KB, human heart and liver tissue-culture cell lines have been studied.The concentration of RNA, protein and phospholipid (mug/mug of DNA) of total subcellular particles was similar for all four cell lines studied. The greatest RNA concentration and lowest protein...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02531999
更新日期:1967-01-01 00:00:00
abstract::The biosynthesis of 3R-hydroxy-5Z, 8Z, 11Z,14Z-eicosatetraenoic acid (3R-HETE) from arachidonic acid (20:4n-6) by the hyphal-forming yeast, Dipodascopsis uninucleata, in cell-free enzyme extracts required CoASH, ATP, NAD+ and Mg2+; 3R-HETE was present as the CoA derivative in enzyme extracts and its biosynthesis was a...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-000-0637-4
更新日期:2000-11-01 00:00:00
abstract::Microtiter plate methods were developed for the enzymatic determination of serum total cholesterol (TC), high density lipoprotein cholesterol (HDL-C) and triglyceride (TG), and for the turbidometric determination of apolipoproteins. The micromethods resulted in accurate, precise values that were in good agreement with...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02535780
更新日期:1993-02-01 00:00:00
abstract::Synthetic activators of peroxisome proliferator-activated receptors (PPAR)-alpha and -gamma are capable of reducing macrophage foam cell cholesterol accumulation through the activation of genes involved in cholesterol homeostasis. Since conjugated linoleic acids (CLA) were also demonstrated to activate PPARalpha and P...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-008-3226-x
更新日期:2008-10-01 00:00:00
abstract::The initial steps in the autoxidation of CLA methyl ester are poorly understood. The aim of this study was to determine the stereochemistry of the hydroperoxides formed during autoxidation of CLA methyl ester in the presence of a good hydrogen atom donor. For this purpose, 9-cis,11-trans CLA methyl ester was autoxidiz...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-002-0929-8
更新日期:2002-06-01 00:00:00
abstract::There have been a limited number of studies investigating surfactant lipid changes in lung with trace elements. The present investigation was designed to examine the effect of moderate zinc deficiency on the lipid metabolism in rat lung. We also evaluated whether zinc deficiency, which is a wide-spread problem, could ...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-002-0893-3
更新日期:2002-03-01 00:00:00
abstract::[1-14C]alpha-Linolenic acid was incubated with a particulate fraction of homogenate of leaves of the meadow buttercup (Ranunculus acris L.). The main product was a divinyl ether fatty acid, which was identified as 12-[1'(Z),3'(Z)-hexadienyloxy]-9(Z),11(E)-dodecadienoic acid. Addition of glutathione peroxidase and redu...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-998-0306-7
更新日期:1998-11-01 00:00:00
abstract::Triacylglycerol (TAG) is the main storage lipid in plants. Acyl-CoA: diacylglycerol acyltransferase (DGAT1 and DGAT2) and phospholipid: diacylglycerol acyltransferase (PDAT) can catalyze TAG synthesis. It is unclear how these three independent genes are regulated in developing seeds, and particularly if they have spec...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-010-3385-4
更新日期:2010-02-01 00:00:00
abstract::The permeability of an N-oleoyldihydrosphingomyelin bilayer against glycerol was similar to that of a bilayer of phosphatidylcholine with identical effective hydrophobic chain length. Cholesterol at 1:1 molar ratio reduced the permeability, and also reduced the energy of activation of glycerol penetration, an effect n...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02533668
更新日期:1978-04-01 00:00:00