Abstract:
PURPOSE:The concentration of nucleic acids that undergo apoptosis (ssDNA) determines the actual activity of programmed cell death. ssDNA concentrations in liver tissue of patients with chronic HBV, HCV and HCV and HIV infections were assessed. The concentration of this nucleic acid was analyzed in relation to the concentrations of serous apoptosis indicators, sFas and sFasL receptor proteins, the activity of inflammatory processes and fibrosis in liver tissue as well as HBV, HCV and HIV viraemia. PATIENTS:The study included 153 patients: 48 chronic HBV infected, 86 chronic HCV infected and 19 HCV and HIV infected. PATIENTS AND METHODS:The concentrations of HBV-DNA, HCV-RNA and HIV-RNA were determined by use of RT-PCR method. CD3+, CD4+ and CD8+ lymphocytes count were detected in HIV infected patients' blood by use of a flow cytometer. The concentration of ssDNA was determined by use of monoclonal antibodies and ELISA tests. The concentrations of sFas and sFasL in serum were determined by use of an immunoenzymatic method (ELISA). RESULTS:The concentration of ssDNA in liver tissue of both HCV and HBV infected patients was higher in comparison to those co-infected with HCV and HIV (1332 x 10(-6) g/mg, +/-664 x 10(-6); vs 1508 x 10(-6) microg/mg, +/-810 x 10(-6); vs 886 x 10(-6) microg/mg, +/- 388 x 10(-6); p < 0.004). No correlation between ssDNA concentration and HBV and HCV viraemia was observed. In patients infected with HCV genotype 3, the concentration of ssDNA was 1343 x 10(-6) microg/mg, +/-700 x 10(-6), comparable from patients infected with genotype 1, 296 x 10(-6) microg/mg, +/- 615 x 10(-6). The highest concentration of ssDNA in liver tissue was detected in HBV infected patients with low inflammatory activity (1645 x 10(-6) microg/mg, +/-987) and low fibrosis (1606 x 10(-6) microg/mg, +/- 876 x 10(-6). Mild inflammatory changes and low fibrosis were observed in all HCV and HIV infected patients. No correlation between ssDNA concentration in liver tissue and HIV viraemia (r = 0.03; p = 0.90), HCV, CD8+ and CD4+ count (r = -11; p = 0.66) was observed. The concentration of ssDNA among HCV and HIV infected patients correlated with the concentration of sFas in serum (r = 0.52; p < 0.02). CONCLUSIONS:HCV, HBV and HIV viraemias do not correlate with ssDNA concentration in liver tissue. In patients with HCV and HIV infections, CD4+ and CD3+ counts do not correlate with the concentration of ssDNA in liver tissue. HIV infection seems to inhibit apoptosis processes in liver tissue of HCV and HIV co-infected patients. In the case of HCV and HIV infections, the concentration of sFas in serum correlates with the concentration of ssDNA in liver tissue.
journal_name
Adv Med Scijournal_title
Advances in medical sciencesauthors
Łapinski TW,Wiercińska-Drapało A,Panasiuk A,Kovalchuk Osubject
Has Abstractpub_date
2007-01-01 00:00:00pages
109-13eissn
1896-1126issn
1898-4002journal_volume
52pub_type
杂志文章abstract:PURPOSE:Non-alcoholic fatty liver disease (NAFLD) is increasingly being recognized in the pediatric population, therefore, the search for non-invasive parameters to predict progression of NAFLD is of great interest. The aim of this study was to assess serum concentration of sCD36 in children with obesity and to determi...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:10.1016/j.advms.2020.11.004
更新日期:2020-12-01 00:00:00
abstract:PURPOSE:The assessment of satisfaction of women after mastectomy for nursing care. MATERIAL AND METHODS:The research was carried out on a group of 217 patients after radical mastectomy and took place at Surgical Clinic of Swietokrzyskie Oncology Centre in Kielce. Authors' assessment scale was used in research. RESULT...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:
更新日期:2007-01-01 00:00:00
abstract:BACKGROUND:Current risk stratification after acute myocardial infarction (MI) depends on left ventricular ejection fraction. Microvolt T-wave alternans (MTWA) is one of promising markers to predict cardiac events in patients after acute MI treated according to current guidelines. METHODS:In this single center study, 1...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:10.2478/v10039-012-0033-7
更新日期:2012-01-01 00:00:00
abstract:PURPOSE:The study aimed to determine the effect of OSA and obesity on the plasma levels of adiponectin and the long-term effect of CPAP on its plasma levels and obesity parameters. MATERIAL/METHODS:A prospective observational study included 159 newly diagnosed OSA patients. The cohort was divided into CPAP treated (n=...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:10.1016/j.advms.2015.10.004
更新日期:2016-03-01 00:00:00
abstract::Lung cancer causes an estimated 1.6 million deaths each year, being the leading cause of cancer-related deaths in the world. Late diagnosis and, in some cases, the high aggressiveness of the tumour result in low overall five-year survival rates of 12% among men and 7% among women. The cure is most likely in early-stag...
journal_title:Advances in medical sciences
pub_type: 杂志文章,评审
doi:10.1016/j.advms.2017.12.002
更新日期:2018-09-01 00:00:00
abstract:PURPOSE:It is essential in the medical care for immunocompromised patients that the environment should create minimal infection risk. Particularly, it concerns fungal infections. Fungi belonging to the Aspergillus genus are second frequent group of nosocomial pathogens producing opportunistic infections. MATERIAL AND ...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:10.2478/v10039-010-0039-y
更新日期:2010-01-01 00:00:00
abstract:PURPOSE:Adaptation of the colorimetric method for the determination of β-d-galactosidase, β-d-glucuronidase and α-l-fucosidase activities in serums from hemolyzed blood, the material currently being discarded. MATERIALS AND METHODS:The materials included serums from hemolyzed and non-hemolyzed blood, obtained from 26 ...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:10.1016/j.advms.2018.04.005
更新日期:2018-09-01 00:00:00
abstract:PURPOSE:The aim of the present study was to assess the effect of tumor advancement and surgery treatment on P-selectin expression (CD 62P), level of sP-selectin and platelet count. MATERIAL AND METHODS:The study involved 27 colorectal cancer patients (CRC). They were divided into two groups: group B1--18 patients (T2-...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:
更新日期:2006-01-01 00:00:00
abstract:PURPOSE:To determine the time point at which thrombocytopenia after TAVI procedure is an indicator of the worst prognosis, with special consideration of perioperative platelet and coagulation activation as its potential causes. METHODS:Thirty two patients (mean age 78.5±7.9years, 62% females) qualified for TAVI proced...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:10.1016/j.advms.2017.11.001
更新日期:2018-03-01 00:00:00
abstract:PURPOSE:Urinary incontinence is a disease due to which women have suffered for many centuries. But there is some optimistic side to the problem, too; such illnesses can be treated and prevented. The aim of the work was to evaluate the frequency of occurring such cases among women and to evaluate the chosen risk factors...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:
更新日期:2007-01-01 00:00:00
abstract:PURPOSE:Anthranilic acid is an important, the aromatic intermediate in the degradation of tryptophan in kynurenine pathway. This compound plays an important role in the regulation of immunological processes as well shows antibacterial activity. The aim of our study was to estimate the concentration of anthranilic acid ...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:
更新日期:2006-01-01 00:00:00
abstract:PURPOSE:To assess the prognostic significance of clinicopathological factors, especially histological parameters of new Jass classification, following sphincter-sparing total mesorectal excision (TME) for high-risk rectal cancer. MATERIAL AND METHODS:Forty-five consecutive patients treated with curative intent in 1998...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:
更新日期:2007-01-01 00:00:00
abstract:PURPOSE:To assess the serum fetuin A concentration as a potential marker of subclinical atherosclerosis in obese children with NAFLD. MATERIAL/METHODS:A prospective analysis of 45 obese children initially diagnosed with liver pathology (elevated serum ALT activity and/or ultrasonographic liver brightness and/or hepato...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:10.1016/j.advms.2013.08.003
更新日期:2014-03-01 00:00:00
abstract:PURPOSE:Glaucoma is one of the most important reason causes of the blindness, associated with retinal ganglion cells (RGC) death. This process is not fully understood, however apoptosis due to hypoxia is one of the most important processes leading to RGC death. Glaucomatous optic neuropathy is characterized by remodeli...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:10.1016/j.advms.2015.07.007
更新日期:2016-03-01 00:00:00
abstract:PURPOSE:The objective of this study was to evaluate thevitamin D concentration in patients with recurrent respiratory infections with or without immunoglobulin G, A or M (IgG, IgA, IgM) deficiency, and to find a correlation between the vitamin D concentration and the response to hepatitis B vaccination. MATERIALS AND ...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:10.1016/j.advms.2017.08.001
更新日期:2018-03-01 00:00:00
abstract:PURPOSE:This pilot study was aimed to establish techniques for assessing and observing trends in endothelial function, antioxidant status and vascular compliance in newly diagnosed HFE haemochromatosis during the first year of venesection. PATIENTS/METHODS:Untreated newly diagnosed HFE haemochromatosis patients were t...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:10.1016/j.advms.2013.07.001
更新日期:2014-03-01 00:00:00
abstract:PURPOSE:Existing anti-epileptic drugs (AED) have limited efficiency in many patients, necessitating the search for alternative approaches such as stem cell therapy. We report the use of autologous patient-derived mesenchymal stem cells (MSC) as a therapeutic agent in symptomatic drug-resistant epilepsy in a Phase I ope...
journal_title:Advances in medical sciences
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.advms.2016.12.004
更新日期:2017-09-01 00:00:00
abstract:PURPOSE:Vitamin A takes part in many physiological and pathological processes in women's reproductive organs. The study objective was to compare the carotenoid content in benign and malignant lesions of the breast, ovary and uterus, and to demonstrate quantitative and qualitative similarities or differences between the...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:10.2478/v10039-008-0047-3
更新日期:2008-01-01 00:00:00
abstract::We present 21 studies of cases of lung cancer in patients with situs inversus totalis (SIT) published worldwide. The first case was described in 1952. Thirteen patients were from Japan, 4 from Eastern Europe, including 2 Polish cases from the authors` center (Department of Thoracic Surgery, Pomeranian Medical Universi...
journal_title:Advances in medical sciences
pub_type: 杂志文章,评审
doi:10.2478/v10039-012-0083-x
更新日期:2013-01-01 00:00:00
abstract:PURPOSE:Glioblastoma is the most common and the deadliest brain cancer. The aim of this study was to analyze the impact of resveratrol and its five analogs: 3,4,4'-trimethoxy, 3,4,2'-trimethoxy, 3,4,2',4'-tetramethoxy, 3,4,2',6'-tetramethoxy and 3,4,2',4',6'-pentamethoxy-trans-stilbenes (MS) on human glioblastoma T98G ...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:10.1016/j.advms.2020.11.001
更新日期:2020-11-22 00:00:00
abstract:PURPOSE:The aim of the study was to evaluate factors related to bone formation and resorption in Polish children and adolescents with cystic fibrosis and to examine the effect of nutritional status, biochemical parameters and clinical status on bone mineral density. MATERIALS AND METHODS:The study group consisted of 1...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:10.1016/j.advms.2015.05.002
更新日期:2015-09-01 00:00:00
abstract:PURPOSE:To investigate the synergistic effects of nerve growth factor (NGF), ciliary neurotrophic factor (CNTF) and glia cell line-derived neurotrophic factor (GDNF) on survival and growth of sensory neurons and motoneurons, as well as on the functional recovery following sciatic nerve injury in rats. METHODS:Experime...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:10.2478/v10039-010-0020-9
更新日期:2010-01-01 00:00:00
abstract:PURPOSE:Caries, a social ailment, is one of the diseases of civilization of the 20th century. In Poland, the incidence rate of caries is very high both in the young and adults. The major etiological factors of caries are: improper oral hygiene, diet based on carbohydrate-rich and highly processed food products, neglect...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:
更新日期:2006-01-01 00:00:00
abstract:PURPOSE:Accumulating evidence has linked long noncoding RNAs (lncRNAs) to autoimmune and inflammatory disorders. This study aimed to detect the expression levels of five lncRNAs (lnc0640, lnc3643, lnc5150, lnc7514 and lncagf) in peripheral blood mononuclear cells (PBMCs) of patients with systemic lupus erythematosus (S...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:10.1016/j.advms.2019.08.002
更新日期:2019-09-01 00:00:00
abstract::Adhesive molecules are responsible for the cell-cell interaction and the surrounding intercellular environment creating normal tissue architecture. The role of adhesion proteins in cancer refers to angiogenesis, loss of tissue continuity, and deprivation of intercellular contact with the extracellular matrix, promotin...
journal_title:Advances in medical sciences
pub_type: 杂志文章,评审
doi:10.1016/j.advms.2019.08.003
更新日期:2019-09-01 00:00:00
abstract:PURPOSE:We aimed to examine the polymorphism of the promoter and exon 5 of the TNFSF11 gene and their impact on bone mineral density (BMD) and the frequency of bone fractures. TNFSF11 encodes the receptor activator of the NF-kB ligand (RANKL), a key regulator of bone metabolism and osteoporosis drug targets. BMD is an ...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:10.1016/j.advms.2020.05.001
更新日期:2020-09-01 00:00:00
abstract:PURPOSE:To determine reference values and tolerance limits of between-side differences for the calibers of the common femoral artery (CFA), superficial femoral artery (SFA), popliteal artery (PA), dorsalis pedis artery (DPA), and posterior tibial artery (PTA). MATERIALS AND METHODS:Calibers of arteries, defined as the...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:10.1016/j.advms.2019.10.001
更新日期:2020-03-01 00:00:00
abstract:PURPOSE:Psoriasis is a chronic, recurrent, inflammatory disease. Recent investigations indicate its autoimmune pathogenesis. Apoptosis plays an important role in the development of many autoimmune diseases. The aim of this study was to evaluate the influence of topical treatment of psoriasis on soluble Fas (sFas) and s...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:10.1016/j.advms.2014.10.005
更新日期:2015-03-01 00:00:00
abstract:PURPOSE:Solid tumors modulate their environment to keep non-malignant stromal cells in a tumor-promoting state. The main cells in the stroma of epithelial derived tumors are cancer associated fibroblasts (CAF) that are critical to tumorigenesis and angiogenesis. CAFs also supply the tumor cells with growth factors and ...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:10.1016/j.advms.2015.04.006
更新日期:2015-09-01 00:00:00
abstract:PURPOSE:The question of whether or not non-sporadic breast malignancies have different immunohistochemical features than sporadic malignancies has not been investigated previously. Consequently, the purpose of this study was to compare the expression of E-cadherin (EC) in breast cancer patients with positive and negati...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:10.2478/v10039-008-0007-y
更新日期:2008-01-01 00:00:00