Abstract:
PURPOSE:Glioblastoma is the most common and the deadliest brain cancer. The aim of this study was to analyze the impact of resveratrol and its five analogs: 3,4,4'-trimethoxy, 3,4,2'-trimethoxy, 3,4,2',4'-tetramethoxy, 3,4,2',6'-tetramethoxy and 3,4,2',4',6'-pentamethoxy-trans-stilbenes (MS) on human glioblastoma T98G cells. MATERIALS AND METHODS:The Parallel Artificial Membrane Permeation Assay (PAMPA) was used for the prediction of blood-brain barrier penetration ability of the tested stilbenes (PAMPA-BBB). MTT test was applied to analyze the cytotoxicity of the compounds, whereas their ability to inhibit Wnt/β-catenin target genes expression was verified using qPCR. The potential DNA demethylating properties of the analyzed compounds were tested by Methylation-Sensitive High Resolution Melting (MS-HRM). Cell cycle distribution was tested using Fluorescence-Activated Cell Sorting (FACS), whereas apoptosis was analyzed using FITC Annexin V/propidium iodide double staining assay and Western blot. RESULTS:High blood-brain barrier permeability coefficient was obtained for both resveratrol as well as methoxy-stilbenes. Their ability to downregulate the expression of Wnt/β-catenin pathway-related genes was confirmed. The 4'-methoxy substituted derivatives showed higher activity, whereas 3,4,4'-tri-MS was the most potent Wnt/β-catenin pathway inhibitor. None of the compounds affected DNA methylation level of MGMT, SFRP1, or RUNX3, despite inducing moderate changes in the level of expression of epigenetic modifiers DNMT3B and TET1-3. Importantly, treatment with 3,4,4'-tri-MS and 3,4,2',4'-tetra-MS led to cycle arrest in the S phase and induced apoptosis. CONCLUSIONS:Both, resveratrol, as well as its synthetic methoxy-derivatives, should be further studied as promising adjuvants in glioblastoma treatment.
journal_name
Adv Med Scijournal_title
Advances in medical sciencesauthors
Majchrzak-Celińska A,Zielińska-Przyjemska M,Wierzchowski M,Kleszcz R,Studzińska-Sroka E,Kaczmarek M,Paluszczak J,Cielecka-Piontek J,Krajka-Kuźniak Vdoi
10.1016/j.advms.2020.11.001subject
Has Abstractpub_date
2020-11-22 00:00:00pages
6-20issue
1eissn
1896-1126issn
1898-4002pii
S1896-1126(20)30041-9journal_volume
66pub_type
杂志文章abstract:PURPOSE:To evaluate the serum levels of tumor necrosis factor (TNF) alpha and its soluble receptors in obese children with non-alcoholic fatty liver disease (NAFLD). MATERIAL/METHODS:Fasting serum levels of TNFalpha and its receptors were determined in 45 consecutive obese children with suspected liver disease and 20 ...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:10.2478/v10039-010-0008-5
更新日期:2010-01-01 00:00:00
abstract:PURPOSE:We aimed to examine the polymorphism of the promoter and exon 5 of the TNFSF11 gene and their impact on bone mineral density (BMD) and the frequency of bone fractures. TNFSF11 encodes the receptor activator of the NF-kB ligand (RANKL), a key regulator of bone metabolism and osteoporosis drug targets. BMD is an ...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:10.1016/j.advms.2020.05.001
更新日期:2020-09-01 00:00:00
abstract:PURPOSE:Methylenetetrahydrofolate reductase (MTHFR) is an enzyme involved in endothelial nitric oxide synthase (eNOS) coupling and homocysteine metabolism. The rs1801133 polymorphism of the MTHFR gene affects risk of coronary artery disease. We assessed its influence on 5-year survival of patients with ST-elevation acu...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:10.2478/v10039-012-0010-1
更新日期:2012-06-01 00:00:00
abstract:PURPOSE:Obstructive sleep apnea increases the risk of cardiovascular diseases. Alternations in prostacyclin and thromboxane concentrations and balance could constitute one of mechanisms linking sleep apnea and cardiovascular events. Thus we aimed to assess the concentrations of 6-keto-prostaglandin F1α (6-keto-PGF1α) (...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:10.1016/j.advms.2015.12.001
更新日期:2016-03-01 00:00:00
abstract:PURPOSE:To characterize the interaction between tiagabine (TGB) and valproate (VPA)--two antiepileptic drugs in the mouse pentylenetetrazole (PTZ)-induced clonic seizure model, type I isobolographic analysis for non-parallel dose-response relationship curves (DRRCs) was used. MATERIAL AND METHODS:Clonic seizures were ...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:10.2478/v10039-009-0006-7
更新日期:2009-01-01 00:00:00
abstract:PURPOSE:The aim of this study was to determine the antimicrobial resistance and the occurrence of virulence determinants among glycopeptide-resistant enterococci (GRE) isolated in 2007-2009 from patients hospitalized in southwestern Poland. MATERIAL AND METHODS:The minimal inhibitory concentrations (MICs) of antibioti...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:10.2478/v10039-011-0022-2
更新日期:2011-01-01 00:00:00
abstract:PURPOSE:Caries, a social ailment, is one of the diseases of civilization of the 20th century. In Poland, the incidence rate of caries is very high both in the young and adults. The major etiological factors of caries are: improper oral hygiene, diet based on carbohydrate-rich and highly processed food products, neglect...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:
更新日期:2006-01-01 00:00:00
abstract:PURPOSE:C-kit positive interstitial cells of Cajal (ICCs) play an important role in the regulation of the smooth muscle motility, acting as pacemakers to provide the slow wave activity in various organs. Recent studies have shown that c-kit positive ICCs are widely distributed in the urinary tract of animals and humans...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:10.2478/ams-2013-0002
更新日期:2013-01-01 00:00:00
abstract:BACKGROUND:Current risk stratification after acute myocardial infarction (MI) depends on left ventricular ejection fraction. Microvolt T-wave alternans (MTWA) is one of promising markers to predict cardiac events in patients after acute MI treated according to current guidelines. METHODS:In this single center study, 1...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:10.2478/v10039-012-0033-7
更新日期:2012-01-01 00:00:00
abstract::Hepatitis C virus (HCV) infection is a common problem. Combined treatment with interferon and ribavirin improved treatment efficacy, but still high percentage of infected patients has not reached virus elimination. It was found that HCV infection course, but also treatment efficacy among other things can depend on pat...
journal_title:Advances in medical sciences
pub_type: 杂志文章,评审
doi:10.2478/v10039-009-0002-y
更新日期:2009-01-01 00:00:00
abstract:PURPOSE:The primary aim of this study is to compare the clinical course and laboratory parameters of acute cholecystitis in patients with diabetes vs. patients without diabetes. MATERIALS AND METHODS:The study involved patients who underwent emergency cholecystectomy in the Department of General, Endocrine and Transpl...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:10.1016/j.advms.2020.08.001
更新日期:2020-09-01 00:00:00
abstract:PURPOSE:To investigate the synergistic effects of nerve growth factor (NGF), ciliary neurotrophic factor (CNTF) and glia cell line-derived neurotrophic factor (GDNF) on survival and growth of sensory neurons and motoneurons, as well as on the functional recovery following sciatic nerve injury in rats. METHODS:Experime...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:10.2478/v10039-010-0020-9
更新日期:2010-01-01 00:00:00
abstract:PURPOSE:Urinary incontinence is a disease due to which women have suffered for many centuries. But there is some optimistic side to the problem, too; such illnesses can be treated and prevented. The aim of the work was to evaluate the frequency of occurring such cases among women and to evaluate the chosen risk factors...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:
更新日期:2007-01-01 00:00:00
abstract:PURPOSE:Accumulating evidence has linked long noncoding RNAs (lncRNAs) to autoimmune and inflammatory disorders. This study aimed to detect the expression levels of five lncRNAs (lnc0640, lnc3643, lnc5150, lnc7514 and lncagf) in peripheral blood mononuclear cells (PBMCs) of patients with systemic lupus erythematosus (S...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:10.1016/j.advms.2019.08.002
更新日期:2019-09-01 00:00:00
abstract:PURPOSE:Adaptation of the colorimetric method for the determination of β-d-galactosidase, β-d-glucuronidase and α-l-fucosidase activities in serums from hemolyzed blood, the material currently being discarded. MATERIALS AND METHODS:The materials included serums from hemolyzed and non-hemolyzed blood, obtained from 26 ...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:10.1016/j.advms.2018.04.005
更新日期:2018-09-01 00:00:00
abstract:PURPOSE:Non-alcoholic fatty liver disease (NAFLD) is increasingly being recognized in the pediatric population, therefore, the search for non-invasive parameters to predict progression of NAFLD is of great interest. The aim of this study was to assess serum concentration of sCD36 in children with obesity and to determi...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:10.1016/j.advms.2020.11.004
更新日期:2020-12-01 00:00:00
abstract:PURPOSE:This pilot study was aimed to establish techniques for assessing and observing trends in endothelial function, antioxidant status and vascular compliance in newly diagnosed HFE haemochromatosis during the first year of venesection. PATIENTS/METHODS:Untreated newly diagnosed HFE haemochromatosis patients were t...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:10.1016/j.advms.2013.07.001
更新日期:2014-03-01 00:00:00
abstract:PURPOSE:It is essential in the medical care for immunocompromised patients that the environment should create minimal infection risk. Particularly, it concerns fungal infections. Fungi belonging to the Aspergillus genus are second frequent group of nosocomial pathogens producing opportunistic infections. MATERIAL AND ...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:10.2478/v10039-010-0039-y
更新日期:2010-01-01 00:00:00
abstract:PURPOSE:We analyzed the usefulness and prognostic value of intraoperative monitoring for identification of the oculomotor (III) and the abducens (VI) nerve in patients with cavernous sinus meningiomas. MATERIAL/METHODS:43 patients diagnosed with cavernous sinus meningiomas were divided according to their topography. F...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:10.1016/j.advms.2014.08.009
更新日期:2015-03-01 00:00:00
abstract:PURPOSE:In the literature, the manifestations of gastric cancer have been described based on all patients. In recent times, interest has focused on the subgroup of young patients. In the following analysis, the subgroup of young patients (< 50y) is compared with an older reference group (≥ 50y). MATERIAL AND METHODS:B...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:10.2478/v10039-012-0052-4
更新日期:2012-01-01 00:00:00
abstract::PET is a successful modality to detect cancer and in recent years has demonstrated a great diagnostic value in large series of tumour types. PET combines high sensitivity and reasonable resolution, and offers the ability to perform whole body scans. 18F-deoxyglucose (FDG)-PET has also been used to diagnose tumours of ...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:
更新日期:2006-01-01 00:00:00
abstract:PURPOSE:The biggest problem with the occurrence of breast cancer is late diagnosis, which is associated with high mortality rates. The aim of the study was to appraise the number of circulating endothelial precursors and the concentration of vascular endothelial growth factor A (VEGF-A) and the soluble forms of its rec...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:10.1016/j.advms.2019.02.001
更新日期:2019-09-01 00:00:00
abstract:PURPOSE:To assess the prognostic significance of clinicopathological factors, especially histological parameters of new Jass classification, following sphincter-sparing total mesorectal excision (TME) for high-risk rectal cancer. MATERIAL AND METHODS:Forty-five consecutive patients treated with curative intent in 1998...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:
更新日期:2007-01-01 00:00:00
abstract:PURPOSE:Behaviour of the vein thrombus is determined by the activity ratio of coagulation factors to factors of fibrinolytic system. The aim of the study is to evaluate activity of some coagulation and fibrinolytic factors in the vein thrombus. MATERIAL AND METHODS:The activity of platelets aggregating factors, tissue...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:
更新日期:2006-01-01 00:00:00
abstract:PURPOSE:Our question was whether two biochemical markers of preeclampsia, E-selectin and P-selectin, keep their prognostic value in particular stages of labour, or they lose it during labour. MATERIAL AND METHODS:The study group consisted of 36 healthy parturient women who gave physiological births (in control 15 heal...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:
更新日期:2007-01-01 00:00:00
abstract:PURPOSE:Existing anti-epileptic drugs (AED) have limited efficiency in many patients, necessitating the search for alternative approaches such as stem cell therapy. We report the use of autologous patient-derived mesenchymal stem cells (MSC) as a therapeutic agent in symptomatic drug-resistant epilepsy in a Phase I ope...
journal_title:Advances in medical sciences
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.advms.2016.12.004
更新日期:2017-09-01 00:00:00
abstract:PURPOSE:The aim of the study was to evaluate factors related to bone formation and resorption in Polish children and adolescents with cystic fibrosis and to examine the effect of nutritional status, biochemical parameters and clinical status on bone mineral density. MATERIALS AND METHODS:The study group consisted of 1...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:10.1016/j.advms.2015.05.002
更新日期:2015-09-01 00:00:00
abstract:PURPOSE:Lipoxygenases (LOX) have been implicated in carcinogenesis, however both pro- and anti-carcinogenic effects have been reported in different cancer models. Using transgenic mice, which specifically overexpress human 15-lipoxygenase (ALOX15) in endothelial cells (EC), we previously demonstrated significant inhibi...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:10.1016/j.advms.2019.11.006
更新日期:2020-03-01 00:00:00
abstract:PURPOSE:The aim of this study was to assess the quality of life of the osteoarthritis (OA) and rheumatoid arthritis (RA) patients of Outpatient Clinic Rehabilitation in Poznań. MATERIAL AND METHODS:The study consisted of 97 OA patients, including 86 women and 11 men. Almost 123 patients with RA included of 102 women a...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:
更新日期:2007-01-01 00:00:00
abstract:PURPOSE:To determine the time point at which thrombocytopenia after TAVI procedure is an indicator of the worst prognosis, with special consideration of perioperative platelet and coagulation activation as its potential causes. METHODS:Thirty two patients (mean age 78.5±7.9years, 62% females) qualified for TAVI proced...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:10.1016/j.advms.2017.11.001
更新日期:2018-03-01 00:00:00