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Cytotoxicity of Aspergillus strains isolated from the neonatal intensive care unit environment.

Abstract：

PURPOSE:It is essential in the medical care for immunocompromised patients that the environment should create minimal infection risk. Particularly, it concerns fungal infections. Fungi belonging to the Aspergillus genus are second frequent group of nosocomial pathogens producing opportunistic infections. MATERIAL AND METHODS:The objective of the study was evaluation of prevalence and cytotoxicity of selected Aspergillus strains isolated from the neonatal intensive care unit environment. During 5 consecutive days, 30 samples of indoor air and 120 imprints from the ward environment were collected. Cytotoxicity of 17 strains was evaluated using a MTT (3-(4,5-dimethylthiazol-2-yl) 2,5 diphenyltetrazolium bromide) test. RESULTS:Mean numbers of fungi in the indoor air varied from 50 to 2370 c.f.u.xm-3 while in the imprints from 0.04 to 8.83 c.f.u.xcm-2. Moulds were isolated from most of the materials. All of the 17 strains subject to MTT test were cytotoxic. CONCLUSION:All of the Aspergillus strains isolated from the neonatal intensive care unit were cytotoxic. Cytotoxic Aspergillus strains present in the environment of immunocompromised patients may be potentially dangerous to them.

journal_name

Adv Med Sci

journal_title

Advances in medical sciences

authors

Gniadek A,Macura AB,Twaruzek M,Grajewski J

doi

10.2478/v10039-010-0039-y

subject

Has Abstract

pub_date

2010-01-01 00:00:00

pages

242-9

issue

2

eissn

1896-1126

issn

1898-4002

pii

S1896-1126(14)60037-7

journal_volume

55

pub_type

杂志文章

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