Abstract：
PURPOSE:Promoter hypermethylation and reduced expression of many genes have been found in gastric cancer. DNA methyltransferases are enzymes potentially affecting promoter hypermethylation. MATERIAL AND METHODS:We analyzed proteins expression of DNA methyltransferase 1 and 3b by immunohistochemistry in 47 surgically resected gastric cancer samples for which clinicopathological characteristics, patient's outcome and methylation status of 11 selected tumor-related genes have been determined. Promoter methylation status of genes was assessed by methylation specific PCR. RESULTS:We found that DNMT1 and 3b were up-regulated in gastric cancer and were detected in 51.1% and 57.4% of cases, respectively. Co-expression of DNMT1 and 3b was detected in 44.7%. Correlations analysis have showed that DNMT1 overexpression was significantly correlated with gastric cancer of intestinal histological type (P=0.01) and with gender of patient (P=0.01). However, there was no correlation between DNMT1 and DNMT3b overexpression in cancer and patients outcome. Moreover, there were no clear relations between the proteins expression of DNMT1 and 3b and DNA methylation status of genes. But co-expression of DNMT1 and DNMT3b was significantly associated with promoter hypermethylation of RAR-β2 (P=0.04). CONCLUSIONS:Results from our study indicate that DNMT1 and 3b were overexpressed and could be involved in gastric tumorigenesis of intestinal histological type in the case of Tunisian patients.
journal_name
Adv Med Scijournal_title
Advances in medical sciencesauthors
Ksiâa F,Ziadi S,Dhiab MB,Gacem RB,Trimeche Mdoi
10.1016/j.advms.2014.10.002subject
Has Abstractpub_date
2015-03-01 00:00:00pages
50-7issue
1eissn
1896-1126issn
1898-4002pii
S1896-1126(14)00091-1journal_volume
60pub_type
杂志文章abstract：PURPOSE:Vascular endothelial growth factor is an important factor in promoting angiogenesis in malignant processes, matrix metalloproteinase-9 in the degradation of extracellular matrix, which enhances metastasis, and tissue inhibitor of metalloproteinase-1 is its inhibitor. The aim of this study was to investigate the...
journal_title：Advances in medical sciences
pub_type： 杂志文章
doi：10.1016/j.advms.2018.07.002
更新日期：2019-03-01 00:00:00
abstract：PURPOSE:To assess the prognostic significance of clinicopathological factors, especially histological parameters of new Jass classification, following sphincter-sparing total mesorectal excision (TME) for high-risk rectal cancer. MATERIAL AND METHODS:Forty-five consecutive patients treated with curative intent in 1998...
journal_title：Advances in medical sciences
pub_type： 杂志文章
doi：
更新日期：2007-01-01 00:00:00
abstract：PURPOSE:The aim of the present study was to assess the effect of tumor advancement and surgery treatment on P-selectin expression (CD 62P), level of sP-selectin and platelet count. MATERIAL AND METHODS:The study involved 27 colorectal cancer patients (CRC). They were divided into two groups: group B1--18 patients (T2-...
journal_title：Advances in medical sciences
pub_type： 杂志文章
doi：
更新日期：2006-01-01 00:00:00
abstract：PURPOSE:Obstructive sleep apnea increases the risk of cardiovascular diseases. Alternations in prostacyclin and thromboxane concentrations and balance could constitute one of mechanisms linking sleep apnea and cardiovascular events. Thus we aimed to assess the concentrations of 6-keto-prostaglandin F1α (6-keto-PGF1α) (...
journal_title：Advances in medical sciences
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abstract：PURPOSE:We aimed to examine the polymorphism of the promoter and exon 5 of the TNFSF11 gene and their impact on bone mineral density (BMD) and the frequency of bone fractures. TNFSF11 encodes the receptor activator of the NF-kB ligand (RANKL), a key regulator of bone metabolism and osteoporosis drug targets. BMD is an ...
journal_title：Advances in medical sciences
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abstract：PURPOSE:Ulcerative colitis (UC) carries a high risk of developing colorectal cancer (CRC). FK506-binding protein 51 (FKBP51) is a key regulator of glucocorticoid resistance and inflammatory tumor microenvironment. This study aimed to investigate the role of FKBP51 in UC-CRC prognosis. MATERIALS AND METHODS:The FKBP51 ...
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更新日期：2021-01-15 00:00:00
abstract：PURPOSE:The biggest problem with the occurrence of breast cancer is late diagnosis, which is associated with high mortality rates. The aim of the study was to appraise the number of circulating endothelial precursors and the concentration of vascular endothelial growth factor A (VEGF-A) and the soluble forms of its rec...
journal_title：Advances in medical sciences
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abstract：PURPOSE:The objective of this study was to evaluate thevitamin D concentration in patients with recurrent respiratory infections with or without immunoglobulin G, A or M (IgG, IgA, IgM) deficiency, and to find a correlation between the vitamin D concentration and the response to hepatitis B vaccination. MATERIALS AND ...
journal_title：Advances in medical sciences
pub_type： 杂志文章
doi：10.1016/j.advms.2017.08.001
更新日期：2018-03-01 00:00:00
abstract：:Vascular calcification (VC) is highly prevalent in patients with chronic kidney disease (CKD) and is strongly associated with cardiovascular mortality and morbidity. Accumulating evidence over the past decade has challenged the hypothesis of close interaction between bone and VC what raises the possibility of a common...
journal_title：Advances in medical sciences
pub_type： 杂志文章,评审
doi：10.1016/j.advms.2016.08.001
更新日期：2017-03-01 00:00:00
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journal_title：Advances in medical sciences
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更新日期：2015-03-01 00:00:00
abstract：PURPOSE:Response to steroids is included in the diagnostic criteria for IgG4-related sclerosing cholangitis (IgG4-SC). To assess how to appropriately conduct steroid trials for IgG4-related SC, we examined the clinical pictures of steroid responsiveness in IgG4-SC patients. MATERIAL AND METHODS:A total of 29 patients ...
journal_title：Advances in medical sciences
pub_type： 临床试验,杂志文章
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journal_title：Advances in medical sciences
pub_type： 杂志文章,多中心研究
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更新日期：2019-09-01 00:00:00
abstract：PURPOSE:The aim of this study was to determine the antimicrobial resistance and the occurrence of virulence determinants among glycopeptide-resistant enterococci (GRE) isolated in 2007-2009 from patients hospitalized in southwestern Poland. MATERIAL AND METHODS:The minimal inhibitory concentrations (MICs) of antibioti...
journal_title：Advances in medical sciences
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更新日期：2011-01-01 00:00:00
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journal_title：Advances in medical sciences
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.advms.2016.12.004
更新日期：2017-09-01 00:00:00
abstract：PURPOSE:The aim of the study was to investigate the voice quality parameters in 72 rheumatoid arthritis (RA) patients and their relationships with the clinical manifestations and disease activity. The control group consisted of 30 healthy subjects. MATERIAL AND METHODS:All RA patients were evaluated by extensive clini...
journal_title：Advances in medical sciences
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doi：10.1016/j.advms.2015.06.004
更新日期：2015-09-01 00:00:00
abstract：PURPOSE:Glaucoma is one of the most important reason causes of the blindness, associated with retinal ganglion cells (RGC) death. This process is not fully understood, however apoptosis due to hypoxia is one of the most important processes leading to RGC death. Glaucomatous optic neuropathy is characterized by remodeli...
journal_title：Advances in medical sciences
pub_type： 杂志文章
doi：10.1016/j.advms.2015.07.007
更新日期：2016-03-01 00:00:00
abstract：PURPOSE:Accumulating evidence has linked long noncoding RNAs (lncRNAs) to autoimmune and inflammatory disorders. This study aimed to detect the expression levels of five lncRNAs (lnc0640, lnc3643, lnc5150, lnc7514 and lncagf) in peripheral blood mononuclear cells (PBMCs) of patients with systemic lupus erythematosus (S...
journal_title：Advances in medical sciences
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更新日期：2019-09-01 00:00:00
abstract：PURPOSE:Chronic obstructive pulmonary disease is the most common chronic lung disease, which may be caused by different pathological processes, including inflammation. Furthermore, signs of changes in thyroid hormone levels are found in some patients. Deiodinases (DIOs) are selenoproteins (enzymes) involved in the synt...
journal_title：Advances in medical sciences
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doi：10.1016/j.advms.2019.04.001
更新日期：2019-09-01 00:00:00
abstract：PURPOSE:The aim of this prospective randomized trial was to assess the influence of the sequence of pulmonary vessel ligation, during anatomic resection, on long term survival in patients with NSCLC. MATERIAL/METHODS:This prospective randomized study included 385 patients treated surgically with lobectomy or pneumonec...
journal_title：Advances in medical sciences
pub_type： 杂志文章,随机对照试验
doi：10.2478/v10039-012-0061-3
更新日期：2013-01-01 00:00:00
abstract：PURPOSE:The study aimed to examine the volumes of cerebellum and the brain stem in children with cerebral palsy (CP). MATERIAL AND METHODS:The present study included 21 children with spastic diplegic CP (11 girls and 10 boys). Twenty-one patients with CP were age- and gender-matched with the control patients. All subj...
journal_title：Advances in medical sciences
pub_type： 杂志文章
doi：
更新日期：2007-01-01 00:00:00
abstract：PURPOSE:Defining the level of protective factors in saliva of patients suffering from decompensated type 2 diabetes. MATERIAL AND METHODS:50 Patients with diagnosis of decompensated type 2 diabetes, including 32 women and 18 men at the age of 57.9±9.2 years. The control group consisted of 50 people among whom there we...
journal_title：Advances in medical sciences
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doi：10.1016/j.advms.2016.06.005
更新日期：2017-09-01 00:00:00
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journal_title：Advances in medical sciences
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更新日期：2017-09-01 00:00:00
abstract：PURPOSE:The question of whether or not non-sporadic breast malignancies have different immunohistochemical features than sporadic malignancies has not been investigated previously. Consequently, the purpose of this study was to compare the expression of E-cadherin (EC) in breast cancer patients with positive and negati...
journal_title：Advances in medical sciences
pub_type： 杂志文章
doi：10.2478/v10039-008-0007-y
更新日期：2008-01-01 00:00:00
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journal_title：Advances in medical sciences
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doi：10.1016/j.advms.2015.05.002
更新日期：2015-09-01 00:00:00
abstract：PURPOSE:The concentration of nucleic acids that undergo apoptosis (ssDNA) determines the actual activity of programmed cell death. ssDNA concentrations in liver tissue of patients with chronic HBV, HCV and HCV and HIV infections were assessed. The concentration of this nucleic acid was analyzed in relation to the conce...
journal_title：Advances in medical sciences
pub_type： 杂志文章
doi：
更新日期：2007-01-01 00:00:00
abstract：PURPOSE:Sarcoidosis affected lungs detected in more than 90% of patients. The relationship between different stages of pulmonary sarcoidosis and pulmonary function tests (PFT) as well as bronchoalveolar lavage fluid (BALF) cells can be established. Geographic and ethnic factors are known to be linked to the specific ch...
journal_title：Advances in medical sciences
pub_type： 杂志文章
doi：10.2478/v10039-008-0014-z
更新日期：2008-01-01 00:00:00
abstract：PURPOSE:The aim of this study was to assess the quality of life of the osteoarthritis (OA) and rheumatoid arthritis (RA) patients of Outpatient Clinic Rehabilitation in Poznań. MATERIAL AND METHODS:The study consisted of 97 OA patients, including 86 women and 11 men. Almost 123 patients with RA included of 102 women a...
journal_title：Advances in medical sciences
pub_type： 杂志文章
doi：
更新日期：2007-01-01 00:00:00
abstract：PURPOSE:To characterize the interaction between tiagabine (TGB) and valproate (VPA)--two antiepileptic drugs in the mouse pentylenetetrazole (PTZ)-induced clonic seizure model, type I isobolographic analysis for non-parallel dose-response relationship curves (DRRCs) was used. MATERIAL AND METHODS:Clonic seizures were ...
journal_title：Advances in medical sciences
pub_type： 杂志文章
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更新日期：2009-01-01 00:00:00
abstract：PURPOSE:The assessment of satisfaction of women after mastectomy for nursing care. MATERIAL AND METHODS:The research was carried out on a group of 217 patients after radical mastectomy and took place at Surgical Clinic of Swietokrzyskie Oncology Centre in Kielce. Authors' assessment scale was used in research. RESULT...
journal_title：Advances in medical sciences
pub_type： 杂志文章
doi：
更新日期：2007-01-01 00:00:00
abstract：PURPOSE:This pilot study was aimed to establish techniques for assessing and observing trends in endothelial function, antioxidant status and vascular compliance in newly diagnosed HFE haemochromatosis during the first year of venesection. PATIENTS/METHODS:Untreated newly diagnosed HFE haemochromatosis patients were t...
journal_title：Advances in medical sciences
pub_type： 杂志文章
doi：10.1016/j.advms.2013.07.001
更新日期：2014-03-01 00:00:00
