Abstract：
PURPOSE:In a retrospective analysis of the prevalence of KRAS mutations in patients with advanced non-small cell lung cancer (NSCLC), we detected a unique and not earlier described case of a double combination of mutations at codons 12 and 13 of the KRAS gene in a patient with lung adenocarcinoma. MATERIAL/METHODS:To determine the molecular characteristics of the infrequent mutation and the mutational status of the KRAS gene in metastatic brain tumors in the same patient, we performed morphological and molecular tests. RESULTS:Molecular analysis of the nature of the double mutation showed that the unique combination of variants is a monoallelic mutation. This type of changes has not yet been registered in the Catalogue of Somatic Mutations in Cancer database. Molecular assessment of the KRAS mutation status in the brain metastatic site in the same patient, showed no mutations in codons 12 and 13. Moreover, we did not find mutation at exon 19 and 21 of EGFR gene, both in primary tumor as well as in secondary metastatic foci in the brain. CONCLUSIONS:The presented case shows an example of KRAS gene molecular mosaicism and heterogeneity of lung adenocarcinoma primary and metastatic tumors. Molecular heterogeneity of lung adenocarcinoma tumors can significantly affect eligibility of patients for targeted therapies.
journal_name
Adv Med Scijournal_title
Advances in medical sciencesauthors
Charkiewicz R,Niklińska W,Zalewski G,Charkiewicz A,Kozlowski M,Sulewska A,Chyczewski Ldoi
10.2478/v10039-012-0080-0subject
Has Abstractpub_date
2013-01-01 00:00:00pages
83-9issue
1eissn
1896-1126issn
1898-4002pii
S1896-1126(14)60175-9journal_volume
58pub_type
杂志文章abstract：:Gastroesophageal reflux disease (GERD) is a problem frequently occurring among physically and intellectually disabled individuals. In this group of patients GERD is often overlooked, since the symptoms are usually non-specific. We present two cases of disabled children, who developed complications of GERD in the form ...
journal_title：Advances in medical sciences
pub_type： 杂志文章
doi：
更新日期：2007-01-01 00:00:00
abstract：PURPOSE:The aim of the study was to evaluate factors related to bone formation and resorption in Polish children and adolescents with cystic fibrosis and to examine the effect of nutritional status, biochemical parameters and clinical status on bone mineral density. MATERIALS AND METHODS:The study group consisted of 1...
journal_title：Advances in medical sciences
pub_type： 杂志文章
doi：10.1016/j.advms.2015.05.002
更新日期：2015-09-01 00:00:00
abstract：PURPOSE:The objective of this study was to evaluate thevitamin D concentration in patients with recurrent respiratory infections with or without immunoglobulin G, A or M (IgG, IgA, IgM) deficiency, and to find a correlation between the vitamin D concentration and the response to hepatitis B vaccination. MATERIALS AND ...
journal_title：Advances in medical sciences
pub_type： 杂志文章
doi：10.1016/j.advms.2017.08.001
更新日期：2018-03-01 00:00:00
abstract：PURPOSE:Glaucoma is one of the most important reason causes of the blindness, associated with retinal ganglion cells (RGC) death. This process is not fully understood, however apoptosis due to hypoxia is one of the most important processes leading to RGC death. Glaucomatous optic neuropathy is characterized by remodeli...
journal_title：Advances in medical sciences
pub_type： 杂志文章
doi：10.1016/j.advms.2015.07.007
更新日期：2016-03-01 00:00:00
abstract：PURPOSE:It is essential in the medical care for immunocompromised patients that the environment should create minimal infection risk. Particularly, it concerns fungal infections. Fungi belonging to the Aspergillus genus are second frequent group of nosocomial pathogens producing opportunistic infections. MATERIAL AND ...
journal_title：Advances in medical sciences
pub_type： 杂志文章
doi：10.2478/v10039-010-0039-y
更新日期：2010-01-01 00:00:00
abstract：PURPOSE:Lipoxygenases (LOX) have been implicated in carcinogenesis, however both pro- and anti-carcinogenic effects have been reported in different cancer models. Using transgenic mice, which specifically overexpress human 15-lipoxygenase (ALOX15) in endothelial cells (EC), we previously demonstrated significant inhibi...
journal_title：Advances in medical sciences
pub_type： 杂志文章
doi：10.1016/j.advms.2019.11.006
更新日期：2020-03-01 00:00:00
abstract：PURPOSE:To determine the time point at which thrombocytopenia after TAVI procedure is an indicator of the worst prognosis, with special consideration of perioperative platelet and coagulation activation as its potential causes. METHODS:Thirty two patients (mean age 78.5±7.9years, 62% females) qualified for TAVI proced...
journal_title：Advances in medical sciences
pub_type： 杂志文章
doi：10.1016/j.advms.2017.11.001
更新日期：2018-03-01 00:00:00
abstract：PURPOSE:In this multicenter retrospective analysis of the Polish Myeloma Group we assessed the real-life application of allogeneic transplantations (alloHCT) in multiple myeloma (MM) outside clinical trials in Poland. METHODS:Anonymized clinical data of patients who underwent alloHCT were retrospectively collected fro...
journal_title：Advances in medical sciences
pub_type： 杂志文章
doi：10.1016/j.advms.2020.08.003
更新日期：2020-09-01 00:00:00
abstract：PURPOSE:The aim of the work was to use and to evaluate the usefulness of the slow variable magnetic fields to aid the treatment of the teeth chosen for extraction. The marginal paradontium of periapical bone of teeth was in a state of extensive destruction. The teeth were chosen for extraction. MATERIAL AND METHODS:13...
journal_title：Advances in medical sciences
pub_type： 杂志文章
doi：
更新日期：2006-01-01 00:00:00
abstract：PURPOSE:Obstructive sleep apnea increases the risk of cardiovascular diseases. Alternations in prostacyclin and thromboxane concentrations and balance could constitute one of mechanisms linking sleep apnea and cardiovascular events. Thus we aimed to assess the concentrations of 6-keto-prostaglandin F1α (6-keto-PGF1α) (...
journal_title：Advances in medical sciences
pub_type： 杂志文章
doi：10.1016/j.advms.2015.12.001
更新日期：2016-03-01 00:00:00
abstract：PURPOSE:Desmin regulates function of mitochondria, T-tubular system and cytosolic Ca(2+) transients. We investigated whether desmin remodeling correlates with diastolic dysfunction and whether progressive desmin abnormalities are accompanied by increasing diastolic dysfunction stages. PATIENTS AND METHODS:Eighty five ...
journal_title：Advances in medical sciences
pub_type： 杂志文章
doi：10.1016/j.advms.2015.01.009
更新日期：2015-03-01 00:00:00
abstract：PURPOSE:Anthranilic acid is an important, the aromatic intermediate in the degradation of tryptophan in kynurenine pathway. This compound plays an important role in the regulation of immunological processes as well shows antibacterial activity. The aim of our study was to estimate the concentration of anthranilic acid ...
journal_title：Advances in medical sciences
pub_type： 杂志文章
doi：
更新日期：2006-01-01 00:00:00
abstract：PURPOSE:To assess the serum fetuin A concentration as a potential marker of subclinical atherosclerosis in obese children with NAFLD. MATERIAL/METHODS:A prospective analysis of 45 obese children initially diagnosed with liver pathology (elevated serum ALT activity and/or ultrasonographic liver brightness and/or hepato...
journal_title：Advances in medical sciences
pub_type： 杂志文章
doi：10.1016/j.advms.2013.08.003
更新日期：2014-03-01 00:00:00
abstract：:In liver surgery, accurate assessments of liver resection volume and anatomical variation are mandatory for preoperative planning of safe curative hepatectomy. In living donor liver transplantation (LDLT), estimation of hepatic venous drainage is important to avoid liver graft and donor residual liver congestion. This...
journal_title：Advances in medical sciences
pub_type： 杂志文章,评审
doi：
更新日期：2006-01-01 00:00:00
abstract：:Lung cancer causes an estimated 1.6 million deaths each year, being the leading cause of cancer-related deaths in the world. Late diagnosis and, in some cases, the high aggressiveness of the tumour result in low overall five-year survival rates of 12% among men and 7% among women. The cure is most likely in early-stag...
journal_title：Advances in medical sciences
pub_type： 杂志文章,评审
doi：10.1016/j.advms.2017.12.002
更新日期：2018-09-01 00:00:00
abstract：PURPOSE:The current study objective was to compare blood platelet indices in preterm newborns (PTN) and full term newborns (FTN). MATERIALS AND METHODS:We introduced to our study 51 PTN (25 females, 26 males) and 55 FTN (25 females, 30 males). Platelet indices were estimated in blood samples collected from the umbilic...
journal_title：Advances in medical sciences
pub_type： 杂志文章
doi：10.2478/v10039-009-0034-3
更新日期：2009-01-01 00:00:00
abstract：PURPOSE:To determine reference values and tolerance limits of between-side differences for the calibers of the common femoral artery (CFA), superficial femoral artery (SFA), popliteal artery (PA), dorsalis pedis artery (DPA), and posterior tibial artery (PTA). MATERIALS AND METHODS:Calibers of arteries, defined as the...
journal_title：Advances in medical sciences
pub_type： 杂志文章
doi：10.1016/j.advms.2019.10.001
更新日期：2020-03-01 00:00:00
abstract：:The increasing demand in studying cellular functions in cultured cells under various levels of oxygen and hydrogen peroxide (H2O2) is only partly fulfilled by conventional approaches such as hypoxia chambers, bolus additions of H2O2 or redox-cycling drugs. This article describes the recently developed enzymatic GOX/CA...
journal_title：Advances in medical sciences
pub_type： 杂志文章
doi：10.2478/v10039-009-0042-3
更新日期：2009-01-01 00:00:00
abstract：PURPOSE:We aimed to examine the polymorphism of the promoter and exon 5 of the TNFSF11 gene and their impact on bone mineral density (BMD) and the frequency of bone fractures. TNFSF11 encodes the receptor activator of the NF-kB ligand (RANKL), a key regulator of bone metabolism and osteoporosis drug targets. BMD is an ...
journal_title：Advances in medical sciences
pub_type： 杂志文章
doi：10.1016/j.advms.2020.05.001
更新日期：2020-09-01 00:00:00
abstract：PURPOSE:To characterize the interaction between tiagabine (TGB) and valproate (VPA)--two antiepileptic drugs in the mouse pentylenetetrazole (PTZ)-induced clonic seizure model, type I isobolographic analysis for non-parallel dose-response relationship curves (DRRCs) was used. MATERIAL AND METHODS:Clonic seizures were ...
journal_title：Advances in medical sciences
pub_type： 杂志文章
doi：10.2478/v10039-009-0006-7
更新日期：2009-01-01 00:00:00
abstract：:Idiopathic retroperitoneal fibrosis (IRF) entirely confined to the pelvic cavity is an extremely rare clinical entity. Herein, is described the case of a 36- year old male who presented with clinical and imaging manifestations of a large pelvic tumor. Exploratory laparotomy revealed a large mass in the right pelvis or...
journal_title：Advances in medical sciences
pub_type： 杂志文章
doi：10.2478/v10039-009-0029-0
更新日期：2009-01-01 00:00:00
abstract：PURPOSE:Defining the level of protective factors in saliva of patients suffering from decompensated type 2 diabetes. MATERIAL AND METHODS:50 Patients with diagnosis of decompensated type 2 diabetes, including 32 women and 18 men at the age of 57.9±9.2 years. The control group consisted of 50 people among whom there we...
journal_title：Advances in medical sciences
pub_type： 杂志文章
doi：10.1016/j.advms.2016.06.005
更新日期：2017-09-01 00:00:00
abstract：:Autoimmune pancreatitis (AIP) is a particular type of pancreatitis that is thought to have an autoimmune etiology. Before therapy for AIP is begun, accurate diagnosis of AIP is necessary. It is important to distinguish AIP from pancreatic cancer. Since there is currently no diagnostic serological marker for AIP, AIP s...
journal_title：Advances in medical sciences
pub_type： 杂志文章,评审
doi：10.2478/v10039-008-0033-9
更新日期：2008-01-01 00:00:00
abstract：PURPOSE:This pilot study was aimed to establish techniques for assessing and observing trends in endothelial function, antioxidant status and vascular compliance in newly diagnosed HFE haemochromatosis during the first year of venesection. PATIENTS/METHODS:Untreated newly diagnosed HFE haemochromatosis patients were t...
journal_title：Advances in medical sciences
pub_type： 杂志文章
doi：10.1016/j.advms.2013.07.001
更新日期：2014-03-01 00:00:00
abstract：PURPOSE:To evaluate the serum levels of tumor necrosis factor (TNF) alpha and its soluble receptors in obese children with non-alcoholic fatty liver disease (NAFLD). MATERIAL/METHODS:Fasting serum levels of TNFalpha and its receptors were determined in 45 consecutive obese children with suspected liver disease and 20 ...
journal_title：Advances in medical sciences
pub_type： 杂志文章
doi：10.2478/v10039-010-0008-5
更新日期：2010-01-01 00:00:00
abstract：PURPOSE:The excess and deficit of serotonin can be the cause of somatic and mental disorders. The aim of this study was to evaluate serotonin levels in blood and ascitic fluid as well as excretion of 5-hydroxyindoleacetic acid (5-HIAA) in urine in patients with hepatic encephalopathy (HE). MATERIAL AND METHODS:The stu...
journal_title：Advances in medical sciences
pub_type： 杂志文章
doi：10.2478/ams-2013-0010
更新日期：2013-01-01 00:00:00
abstract：PURPOSE:The concentration of nucleic acids that undergo apoptosis (ssDNA) determines the actual activity of programmed cell death. ssDNA concentrations in liver tissue of patients with chronic HBV, HCV and HCV and HIV infections were assessed. The concentration of this nucleic acid was analyzed in relation to the conce...
journal_title：Advances in medical sciences
pub_type： 杂志文章
doi：
更新日期：2007-01-01 00:00:00
abstract：PURPOSE:To evaluate intravascular xenotransplantation of macroencapsulated pancreatic islet cells in the process of the treatment of diabetes mellitus type 1 without immunosuppressive therapy. MATERIAL AND METHODS:Xenogenic intravascular transplantation was performed in 19 patients with diabetes mellitus (type 1) havi...
journal_title：Advances in medical sciences
pub_type： 杂志文章
doi：10.2478/v10039-008-0045-5
更新日期：2008-01-01 00:00:00
abstract：UNLABELLED:Factors believed to affect masticatory efficiency include loss of postcanine teeth, bite force, severity of malocclusion, occlusal contact area, body size and oral motor function. THE AIM:to record if there is relationship between masticatory efficiency and the state of dentition at patients whose occlusion...
journal_title：Advances in medical sciences
pub_type： 杂志文章
doi：
更新日期：2006-01-01 00:00:00
abstract：PURPOSE:Cerebral metastases develop in 10-30% of patients with breast cancer (BC) and in around 3.3 to 4% of patients with ovarian cancer (OC). The aim of the multicenter study is to investigate the correlation between the expression of estrogen alpha receptors (ERα), progesterone receptors (PR), human epidermal growth...
journal_title：Advances in medical sciences
pub_type： 杂志文章,多中心研究
doi：10.1016/j.advms.2018.12.007
更新日期：2019-09-01 00:00:00
