Abstract:
PURPOSE:Solid tumors modulate their environment to keep non-malignant stromal cells in a tumor-promoting state. The main cells in the stroma of epithelial derived tumors are cancer associated fibroblasts (CAF) that are critical to tumorigenesis and angiogenesis. CAFs also supply the tumor cells with growth factors and extracellular matrix (ECM) degrading enzymes. They are thus essential for tumor initiation as well as tumor progression and metastasis, suggesting that they represent an ideal cellular target of an integrative tumor therapy. Fibroblast activation protein (FAP) is a well-defined marker, expressed at high levels on the cell surface of CAFs. FAP, a constitutively active serine peptidase with both dipeptidyl peptidase IV (DPP IV) and collagenase/gelatinase activity, promotes malignant and invasive behavior of epithelial cancers. High stromal expression levels of FAP correlate with poor prognosis. FAP is difficult to detect in non-diseased adult tissue, but it is generally expressed at sites of tissue remodeling. MATERIALS AND METHODS:In our experiments, we aimed for a reduction of the pro-tumorigenic activities of CAFs by depleting FAP from fibroblasts growing in a composite environment with epithelial tumor cells. RESULTS:FAP depletion was achieved by two therapeutically relevant approaches: a novel internalizing anti-FAP IgG1 antibody and FAP gene knock-down by siRNA delivery. The antibody effectively removed FAP from the cell surface and was capable of reversing the FAP mediated migratory and invasive capacity. FAP RNA interference was equally effective when compared to the antibody. CONCLUSIONS:Thus, targeting FAP on CAF suppresses pro-tumorigenic activities and may result in a clinically effective reduction of tumor progression and dissemination.
journal_name
Adv Med Scijournal_title
Advances in medical sciencesauthors
Teichgräber V,Monasterio C,Chaitanya K,Boger R,Gordon K,Dieterle T,Jäger D,Bauer Sdoi
10.1016/j.advms.2015.04.006subject
Has Abstractpub_date
2015-09-01 00:00:00pages
264-72issue
2eissn
1896-1126issn
1898-4002pii
S1896-1126(15)00027-9journal_volume
60pub_type
杂志文章abstract:PURPOSE:Among 264 children suspected of GERD, acid GER was confirmed in 138 children on the basis of 24-hour pH monitoring. AIMS OF THE STUDY:Comparative analysis of parameters of 24-hour intraesophageal pH monitoring with dual-channel probe (in proximal channel) in children with acid GER: primary and secondary to cow...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:
更新日期:2007-01-01 00:00:00
abstract:PURPOSE:NT-proBNP and BNP concentrations in CHF correlate with NYHA class and LVEE Little research has been conducted to compare the clinical performance of these two natriuretic peptides in heterogeneous CHF population. Purpose: to evaluate and compare the clinical performance of NT-proBNP and BNP in heterogeneous gro...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:
更新日期:2006-01-01 00:00:00
abstract:PURPOSE:A femoral artery pseudoaneurysm - is the most common complication associated with invasive coronary interventions. The aim of the study was to analyze the effectiveness of various methods used for femoral pseudoaneurysm treatment and to assess how routine use of radial approach leads to reduction of these site ...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:10.2478/v10039-011-0027-x
更新日期:2011-01-01 00:00:00
abstract:PURPOSE:The study aimed to determine the effect of OSA and obesity on the plasma levels of adiponectin and the long-term effect of CPAP on its plasma levels and obesity parameters. MATERIAL/METHODS:A prospective observational study included 159 newly diagnosed OSA patients. The cohort was divided into CPAP treated (n=...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:10.1016/j.advms.2015.10.004
更新日期:2016-03-01 00:00:00
abstract:PURPOSE:Psoriasis is a chronic, recurrent, inflammatory disease. Recent investigations indicate its autoimmune pathogenesis. Apoptosis plays an important role in the development of many autoimmune diseases. The aim of this study was to evaluate the influence of topical treatment of psoriasis on soluble Fas (sFas) and s...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:10.1016/j.advms.2014.10.005
更新日期:2015-03-01 00:00:00
abstract:PURPOSE:Vascular endothelial growth factor is an important factor in promoting angiogenesis in malignant processes, matrix metalloproteinase-9 in the degradation of extracellular matrix, which enhances metastasis, and tissue inhibitor of metalloproteinase-1 is its inhibitor. The aim of this study was to investigate the...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:10.1016/j.advms.2018.07.002
更新日期:2019-03-01 00:00:00
abstract:PURPOSE:The data concerning the relation between subclinical hypothyroidism (SH) and the risk of cardiovascular disease are divergent. We aimed to assess myocardial perfusion in contrast-enhanced echocardiography and intima-media thickness (IMT) in patients with SH. MATERIAL/METHODS:Forty females with SH without sympt...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:10.2478/v10039-012-0068-9
更新日期:2013-01-01 00:00:00
abstract:PURPOSE:Willow herb has been traditionally used in folk medicine and currently it is a potential raw material for production of phytopharmaceuticals. The aim of this work was to determine the highest amount of flavonoids and the highest radical scavenging activity of willow herb, which was collected in different vegeta...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:10.1016/j.advms.2013.10.002
更新日期:2014-03-01 00:00:00
abstract:PURPOSE:Anthranilic acid is an important, the aromatic intermediate in the degradation of tryptophan in kynurenine pathway. This compound plays an important role in the regulation of immunological processes as well shows antibacterial activity. The aim of our study was to estimate the concentration of anthranilic acid ...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:
更新日期:2006-01-01 00:00:00
abstract:PURPOSE:Molecular features of non-small cell lung cancer (NSCLC) in never-smokers are not well recognized. We assessed the expression of genes potentially related to lung cancer etiology in smoking vs. never-smoking NSCLC patients. METHODS:We assayed frozen tumor samples from surgically resected 31 never-smoking and 5...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:10.2478/ams-2013-0025
更新日期:2013-01-01 00:00:00
abstract:PURPOSE:In a retrospective analysis of the prevalence of KRAS mutations in patients with advanced non-small cell lung cancer (NSCLC), we detected a unique and not earlier described case of a double combination of mutations at codons 12 and 13 of the KRAS gene in a patient with lung adenocarcinoma. MATERIAL/METHODS:To ...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:10.2478/v10039-012-0080-0
更新日期:2013-01-01 00:00:00
abstract:PURPOSE:The purpose of this study was to investigate the influence of selective agonists of the retinoid receptor X (RXR) and the retinoid acid receptor (RAR) on bone metabolism in rats. METHODS:Thirty six male Wistar rats were divided into three groups: receiving bexarotene, or tazarotene, or to control group. Serum ...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:10.1016/j.advms.2015.09.001
更新日期:2016-03-01 00:00:00
abstract:PURPOSE:To assess the prevalence of spontaneous bacterial peritonitis (SBP) in asymptomatic patients with decompensated liver cirrhosis. MATERIAL AND METHODS:Patients (pts) with symptoms of decompensation of liver cirrhosis, ascites, and no signs indicating SBP were included to our study. Exclusion criteria include: 1...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:10.2478/v10039-011-0010-6
更新日期:2011-01-01 00:00:00
abstract:PURPOSE:In this multicenter retrospective analysis of the Polish Myeloma Group we assessed the real-life application of allogeneic transplantations (alloHCT) in multiple myeloma (MM) outside clinical trials in Poland. METHODS:Anonymized clinical data of patients who underwent alloHCT were retrospectively collected fro...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:10.1016/j.advms.2020.08.003
更新日期:2020-09-01 00:00:00
abstract:PURPOSE:To characterize the interaction between tiagabine (TGB) and valproate (VPA)--two antiepileptic drugs in the mouse pentylenetetrazole (PTZ)-induced clonic seizure model, type I isobolographic analysis for non-parallel dose-response relationship curves (DRRCs) was used. MATERIAL AND METHODS:Clonic seizures were ...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:10.2478/v10039-009-0006-7
更新日期:2009-01-01 00:00:00
abstract:PURPOSE:Desmin regulates function of mitochondria, T-tubular system and cytosolic Ca(2+) transients. We investigated whether desmin remodeling correlates with diastolic dysfunction and whether progressive desmin abnormalities are accompanied by increasing diastolic dysfunction stages. PATIENTS AND METHODS:Eighty five ...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:10.1016/j.advms.2015.01.009
更新日期:2015-03-01 00:00:00
abstract:PURPOSE:The question of whether or not non-sporadic breast malignancies have different immunohistochemical features than sporadic malignancies has not been investigated previously. Consequently, the purpose of this study was to compare the expression of E-cadherin (EC) in breast cancer patients with positive and negati...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:10.2478/v10039-008-0007-y
更新日期:2008-01-01 00:00:00
abstract:PURPOSE:Vitamin A takes part in many physiological and pathological processes in women's reproductive organs. The study objective was to compare the carotenoid content in benign and malignant lesions of the breast, ovary and uterus, and to demonstrate quantitative and qualitative similarities or differences between the...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:10.2478/v10039-008-0047-3
更新日期:2008-01-01 00:00:00
abstract:PURPOSE:The aim of this study was to check the pro-apoptotic mechanism of prosthetic reconstruction on epithelial cells of the oral mucosa. PATIENTS AND METHODS:The research was carried out on the saliva of healthy patients using prostheses. The sample swabs were stained using the May-Grünwald-Giemsa method and proces...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:10.1016/j.advms.2019.12.010
更新日期:2020-03-01 00:00:00
abstract:PURPOSE:HCV chronic infection still presents a very serious epidemiological and clinical problem. Apart from its cytopathic effect on liver parenchyma, its detrimental effect on lipid and carbohydrate metabolism has recently been emphasized. The aim of the study was to assess lipid and carbohydrate parameters in childr...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:10.2478/v10039-011-0048-5
更新日期:2011-01-01 00:00:00
abstract:PURPOSE:Glaucoma is one of the most important reason causes of the blindness, associated with retinal ganglion cells (RGC) death. This process is not fully understood, however apoptosis due to hypoxia is one of the most important processes leading to RGC death. Glaucomatous optic neuropathy is characterized by remodeli...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:10.1016/j.advms.2015.07.007
更新日期:2016-03-01 00:00:00
abstract::Adhesive molecules are responsible for the cell-cell interaction and the surrounding intercellular environment creating normal tissue architecture. The role of adhesion proteins in cancer refers to angiogenesis, loss of tissue continuity, and deprivation of intercellular contact with the extracellular matrix, promotin...
journal_title:Advances in medical sciences
pub_type: 杂志文章,评审
doi:10.1016/j.advms.2019.08.003
更新日期:2019-09-01 00:00:00
abstract:PURPOSE:It is essential in the medical care for immunocompromised patients that the environment should create minimal infection risk. Particularly, it concerns fungal infections. Fungi belonging to the Aspergillus genus are second frequent group of nosocomial pathogens producing opportunistic infections. MATERIAL AND ...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:10.2478/v10039-010-0039-y
更新日期:2010-01-01 00:00:00
abstract:PURPOSE:Chronic obstructive pulmonary disease is the most common chronic lung disease, which may be caused by different pathological processes, including inflammation. Furthermore, signs of changes in thyroid hormone levels are found in some patients. Deiodinases (DIOs) are selenoproteins (enzymes) involved in the synt...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:10.1016/j.advms.2019.04.001
更新日期:2019-09-01 00:00:00
abstract:PURPOSE:The purpose of the studies performed was an attempt to establish a potential relationship between total antioxidant status of unstimulated whole saliva, patients' ages, oral hygiene status and dental caries. MATERIAL AND METHODS:The study involved 120 non-smokers. Mean age of the study subjects was 18.40 ± 1.7...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:10.2478/v10039-012-0015-9
更新日期:2012-06-01 00:00:00
abstract:PURPOSE:C-kit positive interstitial cells of Cajal (ICCs) play an important role in the regulation of the smooth muscle motility, acting as pacemakers to provide the slow wave activity in various organs. Recent studies have shown that c-kit positive ICCs are widely distributed in the urinary tract of animals and humans...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:10.2478/ams-2013-0002
更新日期:2013-01-01 00:00:00
abstract:PURPOSE:Lipoxygenases (LOX) have been implicated in carcinogenesis, however both pro- and anti-carcinogenic effects have been reported in different cancer models. Using transgenic mice, which specifically overexpress human 15-lipoxygenase (ALOX15) in endothelial cells (EC), we previously demonstrated significant inhibi...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:10.1016/j.advms.2019.11.006
更新日期:2020-03-01 00:00:00
abstract:PURPOSE:To determine the time point at which thrombocytopenia after TAVI procedure is an indicator of the worst prognosis, with special consideration of perioperative platelet and coagulation activation as its potential causes. METHODS:Thirty two patients (mean age 78.5±7.9years, 62% females) qualified for TAVI proced...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:10.1016/j.advms.2017.11.001
更新日期:2018-03-01 00:00:00
abstract:PURPOSE:The aim of the study was to establish whether preoperative serum levels of HE4 and CA125 could be a good predictor for lymphadenectomy in the early stage of endometrioid adenocarcinoma of the uterus. MATERIAL AND METHODS:Preoperative serum HE4 and CA125 were measured in 78 postmenopausal patients treated surgi...
journal_title:Advances in medical sciences
pub_type: 杂志文章
doi:10.1016/j.advms.2015.07.010
更新日期:2016-03-01 00:00:00
abstract:PURPOSE:Response to steroids is included in the diagnostic criteria for IgG4-related sclerosing cholangitis (IgG4-SC). To assess how to appropriately conduct steroid trials for IgG4-related SC, we examined the clinical pictures of steroid responsiveness in IgG4-SC patients. MATERIAL AND METHODS:A total of 29 patients ...
journal_title:Advances in medical sciences
pub_type: 临床试验,杂志文章
doi:10.1016/j.advms.2015.02.006
更新日期:2015-09-01 00:00:00