当前位置： SCI文献检索 > Advances in Medical Sciences期刊下所有文献 > Optimization of the method for α-l-fucosidase, β-d-galactosidase and β-d-glucuronidase determination in serum from hemolyzed blood.

Optimization of the method for α-l-fucosidase, β-d-galactosidase and β-d-glucuronidase determination in serum from hemolyzed blood.

Abstract：

PURPOSE:Adaptation of the colorimetric method for the determination of β-d-galactosidase, β-d-glucuronidase and α-l-fucosidase activities in serums from hemolyzed blood, the material currently being discarded. MATERIALS AND METHODS:The materials included serums from hemolyzed and non-hemolyzed blood, obtained from 26 healthy volunteers. The adaptation of the method involved precipitation of the proteins with trichloroacetic acid after incubating serums with substrates, but before determining the products of enzymatic reactions. RESULTS:In serums from hemolyzed and non-hemolyzed blood of the same persons, we found high correlations among the results obtained using hemolyzed blood (with adapted) and non-hemolyzed blood (with non-adapted) methods. CONCLUSION:We are able to determine the β-d-galactosidase, β-d-glucuronidase and α-l-fucosidase activities in serums from hemolyzed blood (with adapted) and non-hemolyzed blood (with non-adapted) methods, with the same accuracy and precision.

journal_name

Adv Med Sci

journal_title

Advances in medical sciences

authors

Chojnowska S,Ptaszyńska-Sarosiek I,Kępka A,Szajda SD,Waszkiewicz N,Zwierz K

doi

10.1016/j.advms.2018.04.005

subject

Has Abstract

pub_date

2018-09-01 00:00:00

pages

306-311

issue

2

eissn

1896-1126

issn

1898-4002

pii

S1896-1126(18)30065-8

journal_volume

63

pub_type

杂志文章

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