Abstract:
:Astrocytes are complex bushy cells that serve important functions through close contacts between their processes and synapses. However, the spatial interactions and dynamics of astrocyte processes relative to synapses have proven problematic to study in adult living brain tissue. Here, we report a genetically targeted neuron-astrocyte proximity assay (NAPA) to measure astrocyte-synapse spatial interactions within intact brain preparations and at synaptic distance scales. The method exploits resonance energy transfer between extracellularly displayed fluorescent proteins targeted to synapses and astrocyte processes. We validated the method in the striatal microcircuitry following in vivo expression. We determined the proximity of striatal astrocyte processes to distinct neuronal input pathways, to D1 and D2 medium spiny neuron synapses, and we evaluated how astrocyte-to-excitatory synapse proximity changed following cortical afferent stimulation, during ischemia and in a model of Huntington's disease. NAPA provides a simple approach to measure astrocyte-synapse spatial interactions in a variety of experimental scenarios. VIDEO ABSTRACT.
journal_name
Neuronjournal_title
Neuronauthors
Octeau JC,Chai H,Jiang R,Bonanno SL,Martin KC,Khakh BSdoi
10.1016/j.neuron.2018.03.003subject
Has Abstractpub_date
2018-04-04 00:00:00pages
49-66.e9issue
1eissn
0896-6273issn
1097-4199pii
S0896-6273(18)30180-6journal_volume
98pub_type
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