Abstract：
:Endoplasmic reticulum (ER) and mitochondria dysfunction contribute to insulin resistance generation during obesity and diabetes. ER and mitochondria interact through Mitofusin 2 (MTF2), which anchors in the outer mitochondrial and ER membranes regulating energy metabolism. Ablation of MTF2 leads to ER stress activation and insulin resistance. Here we determine whether lipotoxic insult induced by saturated lipids decreases MTF2 expression leading to ER stress response in hypothalamus and its effects on insulin sensitivity using in vitro and in vivo models. We found that lipotoxic stimulation induced by palmitic acid, but not the monounsaturated palmitoleic acid, decreases MTF2 protein levels in hypothalamic mHypoA-CLU192 cells. Also, palmitic acid incubation activates ER stress response evidenced by increase in the protein levels of GRP78/BIP marker at later stage than MTF2 downregulation. Additionally, we found that MTF2 alterations induced by palmitic, but not palmitoleic, stimulation exacerbate insulin resistance in hypothalamic cells. Insulin resistance induced by palmitic acid is prevented by pre-incubation of the anti-inflammatory and the ER stress release reagents, sodium salicylate and 4 phenylbutirate, respectively. Finally, we demonstrated that lipotoxic insult induced by high fat feeding to mice decreases MTF2 proteins levels in arcuate nucleus of hypothalamus. Our data indicate that saturated lipids modulate MTF2 expression in hypothalamus coordinating the ER stress response and the susceptibility to insulin resistance.
journal_name
Brain Resjournal_title
Brain researchauthors
Diaz B,Fuentes-Mera L,Tovar A,Montiel T,Massieu L,Martínez-Rodríguez HG,Camacho Adoi
10.1016/j.brainres.2015.09.014subject
Has Abstractpub_date
2015-11-19 00:00:00pages
80-9eissn
0006-8993issn
1872-6240pii
S0006-8993(15)00712-Xjournal_volume
1627pub_type
杂志文章相关文献
BRAIN RESEARCH文献大全abstract：:We investigated the effect of ketogenic diet (KD) on clusterin accumulation in the kainic acid (KA)-induced seizure model. Two days after KA administration, strong clusterin-like immunoreactivity (IR) was detected in the hippocampus in the normal diet (ND)-fed mice. But in the KD-fed mice, few clusterin-like IR was de...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2005.01.097
更新日期：2005-04-25 00:00:00
abstract：:In recent years, we have carried out several studies on brainstem mechanisms associated with pain modulation. Of particular interest has been the region of the nucleus tractus solitarius (NTS) at the level of the area postrema (AP) in the dorsomedial medulla. This region has been found to be rich in opiate receptors a...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(83)91362-8
更新日期：1983-07-18 00:00:00
abstract：:We have characterized the effects of retinoic acid (RA) on the growth, morphology and biosynthesis of cytoskeletal proteins in NB2a mouse neuroblastoma cells. In addition, the morphological and biochemical changes were compared to those induced by dibutyryl cyclic AMP (db cAMP). Growth inhibition by RA was concentrati...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0165-3806(85)90220-2
更新日期：1985-08-01 00:00:00
abstract：:Maternal seizure has adverse effects on brain histology as well as on learning and memory ability in progeny. An enriched environment (EE) is known to promote structural changes in the brain and improve cognitive and motor deficits following a variety of brain injuries. Whether EE treatment in early postnatal periods ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2012.06.034
更新日期：2012-08-27 00:00:00
abstract：:Electrolytic lesions were placed in the medial cerebellar nucleus in tree shrews (Tupaia glis) or in fibers issuing from this nucleus. Brains and spinal cords were processed according to Fink-Heimer procedure following survival times of 2-7 days. In control animals lesions were placed in the cerebellar cortex and, in ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(79)91044-8
更新日期：1979-08-10 00:00:00
abstract：:A qualitative immunohistochemical study was performed on calcineurin A- and calbindin-positive neurons in the spinal cord of transgenic mice, an animal model of amyotrophic lateral sclerosis, carrying the G93A mutation of the Cu/Zn-superoxide dismutase gene. The results show that calcineurin A-immunoreactive motoneuro...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(00)03048-1
更新日期：2001-01-19 00:00:00
abstract：:Studies of first (L1) and second (L2) language representation in the brain have not identified the timing and locations of neural regions involved in L1 and L2 processing. Magnetoencephalography offers high spatial and temporal resolution and can be employed to disentangle subtle timing and neural control differences ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2012.05.054
更新日期：2012-07-27 00:00:00
abstract：:Intracellular recordings and labelings with horseradish peroxidase (HRP) of expiratory (E) neurons were performed in decerebrate, paralyzed, and ventilated rats. A total of 37 neurons were recorded, from which 4 cells and 1 axon were labeled. They were located in two regions of the ventrolateral medulla. One was in th...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(92)90687-5
更新日期：1992-04-03 00:00:00
abstract：:Parkinson's disease (PD) is a common neurodegenerative disease characterized by progressive loss of midbrain dopaminergic neurons. To gain an insight into the mechanisms underlying the progression of PD, gene expression analysis was performed using two different brain regions, the substantia nigra pars compacta (SN) a...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2010.05.066
更新日期：2010-07-30 00:00:00
abstract：:The pathogenic mechanisms of gene-environment interactions determining variability of human neurodevelopmental disorders remain unclear. In the two consecutive papers, we used the neonatal Borna disease virus (BDV) infection rat model of neurodevelopmental damage to evaluate brain pathology, monoamine alterations, beh...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(02)02723-3
更新日期：2002-07-19 00:00:00
abstract：:It has been reported that training affects motor responses evoked electrically from the dorsomedial frontal cortex (DMFC). Once a monkey had been trained to generate visually-guided saccadic eye movements of a particular size and direction, electrical stimulation of the DMFC elicited saccades of only that size and dir...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(00)02663-9
更新日期：2000-09-15 00:00:00
abstract：:Ibogaine, an indolalkylamine, has been proposed for use in treating stimulant addiction. In the present study we sought to determine if ibogaine had any effects on the neurochemical and motor changes induced by D-amphetamine that would substantiate the anti-addictive claim. Ibogaine (40 mg/kg, i.p.) injected 19 h prio...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(92)90745-u
更新日期：1992-05-01 00:00:00
abstract：:The localization of prostaglandin endoperoxide synthase in monkey brain was investigated by the immunoperoxidase method using the monoclonal antibody (PES-7) raised against the enzyme purified from bovine seminal vesicle. The frozen sections with 30-microns thickness were employed after the brain was fixed with perfus...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(91)91043-z
更新日期：1991-03-08 00:00:00
abstract：:Blink movement metrics were studied in three alert cynomolgus monkeys using electromagnetic search coils attached to the eyelids bilaterally. Monkey blinks averaged approximately 24 degrees. Down-phase peak velocities were approximately twice those of the corresponding up phases. Most blinks were symmetrical with clos...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(93)90869-o
更新日期：1993-04-23 00:00:00
abstract：:A novel and convenient assay was used to determine the effect of recombinant Interleukin-2 (IL-2) on the function of the blood-brain barrier (BBB). The assay is based on a variant of the West Nile virus, WN-25, which had lost its neuroinvasiveness but not its neurovirulence. WN-25, when injected intravenously, can cau...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(89)91079-2
更新日期：1989-09-04 00:00:00
abstract：:Morphine chlorhydrate (0.25 mg/kg)produced a significant and constant reinforcement of post-discharge inhibition of alpha-motoneurons in both normal and paraplegic humans. This effect was reversed by naloxone hydrochloride (0.02 mg/kg). These data do not allow to differentiate the effects of morphine on recurrent inhi...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(81)90961-6
更新日期：1981-10-05 00:00:00
abstract：:A monoclonal antibody was made using the spleen cells of a mouse immunized with chick synaptic membranes and designated as mAb 1D12. It immunoprecipitated 25% of the omega-conotoxin binding protein but no dihydropyridine binding protein solubilized from chick brain membranes. By immunoblotting, a polypeptide of 58-kDa...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(91)90942-o
更新日期：1991-06-14 00:00:00
abstract：:Clinically, phenytoin is thought to be useful in controlling seizure activity by preventing it's spread from the focus to neighboring tissue. Experimentally, it has been suggested that phenytoin's principal action is no longer polysynaptic pathways with primary foci affected less than surrounding tissue. In this study...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(84)90994-6
更新日期：1984-03-19 00:00:00
abstract：:Antimitotic drugs used in the chemotherapeutic treatment of cancers induce undesirable but unavoidable side-effects from interruption of normal mitotic processes throughout the body. We have examined whether several such drugs capable of penetrating the blood-brain barrier - thioTEPA and 5-fluorouracil - influence the...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2006.06.093
更新日期：2006-09-21 00:00:00
abstract：:To examine the structural interactions between synaptically connected neurons in Aplysia, we have developed a method for simultaneously labeling two identified cell with different and compatible intraneuronal marking agents (horseradish peroxidase and [3H]N-acetyl-D-galactosamine) visible in both the light and electro...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(79)90621-8
更新日期：1979-09-14 00:00:00
abstract：:Partial, unilateral olfactory nerve section was performed in mice, and the behavior of the olfactory marker protein (OMP) studied, after this experimental manipulation, with the peroxidase--antiperoxidase method. The protein, which in normal mice is present only in mature olfactory sensory neurons, after unilateral le...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(81)90162-1
更新日期：1981-03-30 00:00:00
abstract：:Mammalian target of rapamycin (mTOR) controls protein translation and has an important role in the mechanism of pain hypersensitivity under persistent pain conditions. However, its expression and localization in pain-related regions of the nervous system is not completely understood. Here, we examined the expression a...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2010.04.010
更新日期：2010-06-08 00:00:00
abstract：:Cocaine (25 mg/kg i.p.) produces analgesia in the rat within 5 min and for a duration of 90 min as determined by the formalin test or for 30 min as determined by the hot plate test. Cocaine analgesia is unaffected by doses of naloxone that are sufficient to attenuate morphine analgesia in both tests. Chlorpromazine (3...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(89)91633-8
更新日期：1989-02-13 00:00:00
abstract：:Immobilization of frogs for 1 h induces analgesia which is blocked but not reversed by low doses of naloxone. After 9 days of daily immobilization for 1 h, the treatment fails to cause analgesia thus indicating that tolerance has developed. Animals tolerant to immobilization-induced analgesia do not show cross-toleran...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(84)90073-8
更新日期：1984-04-02 00:00:00
abstract：:Nestin is an embryonic intermediate filament that transiently expressed in the neural stem/progenitor cells in the developing central nervous system (CNS). Growing evidence has shown that abundant expression of nestin also occurs in both pathological glial-derived tumor cells and reactive astrocytes in various CNS inj...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2007.11.026
更新日期：2008-02-27 00:00:00
abstract：:The effects of local and global context on concurrent sound segregation were examined using a mistuned harmonic paradigm. Presented with the same sounds over three conditions, participants were asked to either categorise the incoming complex according to pitch or harmonicity (active listening) or watch a muted subtitl...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2007.10.093
更新日期：2008-01-16 00:00:00
abstract：:Amphetamine releases dopamine through a transporter-mediated mechanism. The purpose of this report was to further our understanding of the intracellular pool from which amphetamine releases dopamine: the cytoplasmic pool, the vesicular pool, or both. Rats were treated with D-amphetamine (AMPH) (1.0 or 10.0 mg/kg) or a...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(98)00720-3
更新日期：1998-09-21 00:00:00
abstract：:Using cervical sympathectomized and immobilized rabbits, the relationship between the luminance intensity and the magnitude of the response in pupillo-constrictor units of the ciliary nerve was investigated. It was revealed that the intensity--response relationship of the units closely resembled that of retinal recept...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(80)90787-8
更新日期：1980-11-10 00:00:00
abstract：:The effects of morphine sulfate and two benzodiazepine derivatives, chlordiazepoxide HCl and diazepam, were evaluated upon single unit activity of the amygdaloid nuclear complex and hippocampal formation in immobilized cats. All surgical procedures were performed under halothane anesthesia and all wound margins were i...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(77)90595-9
更新日期：1977-05-13 00:00:00
abstract：:Various chemical and physical lesions were employed to elucidate the cellular localization of cholecystokinin (CCK) receptors in the rat caudate nucleus. A wide knife cut severing all caudal afferents reduced caudate CCK receptors by approximately 25%, indicating that some receptors lie on axons and (or) terminals of ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(81)90284-5
更新日期：1981-08-24 00:00:00
