Abstract：
:Nestin is an embryonic intermediate filament that transiently expressed in the neural stem/progenitor cells in the developing central nervous system (CNS). Growing evidence has shown that abundant expression of nestin also occurs in both pathological glial-derived tumor cells and reactive astrocytes in various CNS injuries, implying that nestin may play a crucial role in cell growth or proliferation of astrocyte-derived tumor cells. In the present study, we have investigated the possible role of nestin expression in cell growth or survival of CNS tumor cells by using novel small interfering RNA (siRNA) method in cell culture of rat astrocytoma C6 cell line. The nestin expression and cell growth of the cultured astrocytoma cells were examined after nestin siRNA duplex was delivered by cell transfection for 6 h and cell culture was maintained for 48 h. It revealed an effective suppression influence of nestin siRNA on cell growth of cultured astrocytoma cells in a dose-dependent manner. Quantitative data analysis showed that the doses of nestin siRNA at 30-120 nM significantly decreased both cell numbers and expression levels of nestin mRNA and protein. The nestin siRNA also suppressed expression of cellular glial fibrillary acid protein but showed no obvious influence on expression level of Ki-67 protein (a cell proliferation marker). This study has provided in vitro evidence that nestin siRNA can effectively block nestin expression and reduce cell growth of the cultured C6 astrocytoma cells, strongly suggesting that nestin siRNA-induced suppression of tumor cell growth may provide a potential novel clinical therapy against CNS astroglioma events.
journal_name
Brain Resjournal_title
Brain researchauthors
Wei LC,Shi M,Cao R,Chen LW,Chan YSdoi
10.1016/j.brainres.2007.11.026subject
Has Abstractpub_date
2008-02-27 00:00:00pages
103-12eissn
0006-8993issn
1872-6240pii
S0006-8993(07)02743-6journal_volume
1196pub_type
杂志文章相关文献
BRAIN RESEARCH文献大全abstract：:Anesthetized Sprague-Dawley rats fitted with intracerebroventricular (i.c.v.) cannulas were infused with one of the aminopeptidase inhibitors, amastatin or bestatin, over a 5-min period. After infusion, 1-2 X 10(6) cpm of [125I]angiotensin II ([125I]AII) or [125I]angiotensin III ([125I]AIII) was injected through the s...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(88)91279-6
更新日期：1988-05-17 00:00:00
abstract：:Following electrophysiological techniques we investigated whether wallerian degeneration of the cat sural nerve may be influenced by (1) its peripheral connection and (2) the distance from the cell body. Distal stumps connected to their innervation sites peripherally showed less degeneration than proximal ones when is...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(90)91332-b
更新日期：1990-08-13 00:00:00
abstract：:Several lines of evidence support a role for nerve growth factor (NGF) in the sympathetic sprouting response that occurs following septal cholinergic denervation of the rat hippocampal formation. The present study was undertaken to compare the distribution of NGF-like immunoreactivity and the topography of sympathetic...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(94)01344-h
更新日期：1995-02-20 00:00:00
abstract：:In nondiabetic animals, estrogen has been shown to provide significant neuroprotection in focal and transient forebrain ischemia models. However, that neuroprotection may be diminished or lost in the diabetic. In this study, we compared the level of brain damage in intact, ovariectomized (OVX) and 17beta-estradiol (E(...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(02)03484-4
更新日期：2002-11-22 00:00:00
abstract：:By means of silver impregnation and an HRP method, courses of the accessory optic tract were examined in albino and pigmented rats. The accessory optic tract is composed of 3 fasciculi: anterior, lateral and dorsal. The anterior fasciculus gives off fibers to the subthalamic nucleus and terminates in the medial termin...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(83)90960-5
更新日期：1983-06-13 00:00:00
abstract：:Activation of serotonin neurotransmission produces increase of plasma oxytocin, prolactin, vasopressin and ACTH levels. Using dual-immunocytochemistry, the number of oxytocinergic neurons in the hypothalamic magnocellular nuclei co-storing Fos after administration of D-fenfluramine was found to be 15-fold higher compa...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(99)01953-8
更新日期：1999-12-18 00:00:00
abstract：:The ability to detect moving objects is crucial for survival and is a basic capability in the animal kingdom. In the optic tectum, a major center of the visual pathway of birds, neurons have been found that seem to be perfectly suited to encode motion. These cells, located in the stratum griseum centrale, receive a di...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2016.01.022
更新日期：2016-03-15 00:00:00
abstract：:Previous work from this laboratory has documented that glutamate receptor activation and extracellular calcium entry into hippocampal neurons caused a long-lasting down-regulation of ligatin mRNA and protein. Here, we investigated whether glutamate reduced ligatin mRNA levels by decreasing the transcriptional activity...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(95)00599-l
更新日期：1995-09-25 00:00:00
abstract：:We examined the effect of nitric oxide (NO) on the expression of phosphorylated cAMP response element binding (pCREB) protein and on the polysialylated form of the neural cell adhesion molecule (PSA-NCAM), in the hippocampus of the adult rat, by administering 7-nitroindazole (7-NI) for 5 days, to block neuronal nitric...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2004.01.055
更新日期：2004-05-15 00:00:00
abstract：:The participation of glutamatergic circuits of the ventral basal ganglia in feeding-related regulatory mechanisms has been extensively indicated in primate and rodent species. In avian species, it has been shown that ICV injections of MK-801 or of CNQX increase food intake and reduce the latency of feeding initiation ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(02)04196-3
更新日期：2003-03-14 00:00:00
abstract：:To determine whether the idiosyncratic distribution of transduction mechanisms for bitter tastants in rat taste receptor cells (TRCs) could be inferred from the neural activity they evoke, single neuron responses to ten bitter-tasting compounds were recorded from rat glossopharyngeal (n = 30) and chorda tympani (n = 2...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(97)00131-5
更新日期：1997-05-09 00:00:00
abstract：:Zinc ions seem to be important to several neurological functions and have been connected to the pathophysiology of epilepsy, neuronal cell death after seizure or stroke, and Alzheimer's disease. Both epilepsy and Alzheimer's disease are clinical conditions believed to involve the olfactory bulb. The mammalian olfactor...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(02)03544-8
更新日期：2002-11-29 00:00:00
abstract：:Specific binding of [3H](+-)-3-(2-carboxypiperazin-4-yl)propyl-1-phosphonic acid (CPP), a highly selective antagonist for N-methyl-D-aspartic acid (NMDA) receptors, was examined in brain synaptic membranes treated with Triton X-100 by using a filtration assay method. Elevation of incubation temperature from 2 to 30 de...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(90)90575-v
更新日期：1990-05-07 00:00:00
abstract：:The research question was, do events arising from rhythmic passive movement of the human leg lead to inhibition of the H reflex pathway in the stationary leg contralateral to that movement? Further, as the angular velocity of the passive movement increases, does the contralateral reflex inhibition also increase? Stabl...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(93)91303-a
更新日期：1993-02-12 00:00:00
abstract：:The effects of two drugs with anxiolytic actions, diazepam (0.1, 0.3 and 1 mg/kg) and nicotine (0.1 and 0.5 mg/kg) were examined on the time spent in social interaction by pairs of male gerbils. In a test arena lit by high light, diazepam (0.1 mg/kg) increased social interaction, without changing locomotor activity. D...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(00)03102-4
更新日期：2001-01-12 00:00:00
abstract：:Because the occurrence of rapid eye movement (REM) sleep is critically dependent upon an animal's thermal balance, acute pharmacological treatments may effect REM sleep through effects on thermoregulation. We describe how manipulating ambient temperature (T(a)) alters the REM sleep-suppressing effects of systemically-...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(94)90184-8
更新日期：1994-02-04 00:00:00
abstract：:The active and passive membrane properties of developing Purkinje neurons in control cultures and cultures chronically treated with 20 or 40 mM ethanol for 1 or 2 weeks were examined using whole-cell current-clamp techniques. The membrane properties were characterized by the features of the voltage responses evoked by...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(91)90707-3
更新日期：1991-08-30 00:00:00
abstract：:Novel environments induce a conflicting emotional approach-withdrawal state that triggers stress-related reactions. Social support through the presence of a highly familiar conspecific buffers the individual against such challenges. Although aversive events seem to be predominantly processed by the right hemisphere, t...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2020.147198
更新日期：2021-01-15 00:00:00
abstract：:Rats were trained to lever-press for lateral hypothalamic electrical stimulation, and tested following ventral tegmental microinjections of morphine, delta ([D-Pen2,D-Pen5]enkephalin: DPDPE), or kappa (U-50, 488H) receptor agonists or saline. Across the range of effective stimulation frequencies, morphine (8 nmol) and...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(87)90821-3
更新日期：1987-10-13 00:00:00
abstract：:Long-term potentiation (LTP) was demonstrated in a slice preparation of piriform (olfactory) cortex. LTP could be reliably induced in both afferent and association fiber pathways. The magnitude of the observed potentiation was greater in the association fiber pathway. 2-Amino-5-phosphonovalerate (APV) blocked inductio...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(90)91337-g
更新日期：1990-08-13 00:00:00
abstract：:Extended incubation of hippocampus slices in the presence of fatty acid-free bovine serum albumin (FAF-BSA) strongly enhanced the release of D-[3H]aspartate and [14C]GABA induced by veratridine. Saturation of the FAF-BSA with oleic acid abolished the enhancing effect. Spontaneous release and K+-induced release were no...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(88)90687-7
更新日期：1988-08-09 00:00:00
abstract：:Experiments were carried out in rats to (1) elaborate upon the sepcificity of drug action in the periaqueductal gray matter (PAG), and (2) to evaluate the posible congruence of PAG sites of morphine-induced and stimulation-produced analgesis (SPA) applied at virtually identical PAG loci. It was demonstrated that the e...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(77)90886-1
更新日期：1977-03-25 00:00:00
abstract：:The purpose of this study was to examine mechanisms involved in the coupling of neuronal activity to cerebral blood flow (CBF). CBF was measured in rat cerebellum using laser-Doppler flowmetry during stimulus-evoked neuronal activity and related to the distribution of the extracellular field potential. Local electrica...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(95)01354-7
更新日期：1996-02-26 00:00:00
abstract：:The medial amygdala plays a key role in regulating adult social behavior and undergoes structural changes during puberty that may be driven by gonadal hormone secretion during this developmental period. The current study sought to investigate potential organizational effects of testosterone during puberty, activationa...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2012.04.035
更新日期：2012-06-15 00:00:00
abstract：:Zebrafish as a neurogenetic model system depends on the correct neuroanatomical understanding of its brain organization. Here, we address the unresolved question regarding a possible zebrafish homologue of the dorsal pallial division, the region that in mammals gives rise to the isocortex. Analyzing the distributions ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2010.12.089
更新日期：2011-03-24 00:00:00
abstract：:We examined the brain uptake of prostaglandin D2 after its i.v. administration to mice. The determination of this prostaglandin in tissues was performed by a sensitive and rapid radioimmunoassay after purification of the prostaglandin by high-performance liquid chromatography. When 1.0 mg/kg of prostaglandin D2 was in...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(86)91079-6
更新日期：1986-10-22 00:00:00
abstract：:We examined the expression of IL-6 in the substantia nigra pars reticulata (SNr) at various time points after transient (3 h) middle cerebral artery occlusion (MCAO) in rats. The animals were killed at 1, 3, 7 or 14 days following operation. Coronal brain sections were processed for immunohistochemistry with antibodie...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(00)03129-2
更新日期：2001-01-19 00:00:00
abstract：:The amygdala is a bilateral temporal lobe brain region which plays an important role in emotional processing. Past studies on the amygdala have shown hemispheric differences in amygdalar processes and responses associated with specific pain and fear behaviors. Despite the functional differences in the amygdala, few st...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2017.10.028
更新日期：2018-01-01 00:00:00
abstract：:The core neuropsychological processes underlying post-traumatic stress disorder (PTSD) have yet to be elucidated, and the association between anatomical and functional deficits in PTSD remains largely unknown. The aim of our study was to investigate the alterations in cortical thickness and amplitude of low-frequency ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2012.10.048
更新日期：2013-01-15 00:00:00
abstract：:Sardinian alcohol non-preferring (sNP) rats, selected for their low ethanol preference and consumption, carry a point mutation (R100Q) in the gene coding for GABA(A) receptor alpha(6) subunit, which becomes more sensitive to diazepam-evoked GABA currents. We performed binding studies in the cerebellum of normal (RR) a...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(02)04230-0
更新日期：2003-03-28 00:00:00
