Abstract:
:A monoclonal antibody was made using the spleen cells of a mouse immunized with chick synaptic membranes and designated as mAb 1D12. It immunoprecipitated 25% of the omega-conotoxin binding protein but no dihydropyridine binding protein solubilized from chick brain membranes. By immunoblotting, a polypeptide of 58-kDa was identified as the antigen of this antibody in chick, rat, rabbit and guinea pig brain. Immunohistochemical observation indicated the immunoreactivity of mAb 1D12 to be localized in the synaptic regions of central and peripheral neurons. In peripheral organs, there was additional staining in the distal portions of nerve fibers. Immunoelectron microscopy showed immunoreactivity to be located in synaptic vesicle and presynaptic plasma membranes. In the subcellular fractionation of rat brain, 58-kDa protein was recovered in the fractions of synaptic vesicles and plasma membranes but not soluble proteins. This protein could be extracted from membranes by Triton X-100 but treatment with EDTA, acid, base or high salt failed to have such effect. Solubilized 58-kDa protein of rat brain was purified by immunoaffinity chromatography using mAb 1D12. Both protein kinase C and Ca2+/calmodulin-dependent protein kinase II (CaM kinase II) phosphorylated purified 58-kDa protein, and maxima of 0.47 and 0.94 mol of phosphates, respectively, were incorporated per mol of 58-kDa protein. 58-kDa protein was not phosphorylated by either cAMP-dependent or cGMP-dependent protein kinase. When present in membranes, it was also phosphorylated by protein kinase C and CaM kinase II. Possible involvement of 58-kDa protein in the protein kinase C and CaM kinase II-mediated regulation of synaptic transmission in central and peripheral neurons is discussed.
journal_name
Brain Resjournal_title
Brain researchauthors
Takahashi M,Arimatsu Y,Fujita S,Fujimoto Y,Kondo S,Hama T,Miyamoto Edoi
10.1016/0006-8993(91)90942-osubject
Has Abstractpub_date
1991-06-14 00:00:00pages
279-92issue
1-2eissn
0006-8993issn
1872-6240pii
0006-8993(91)90942-Ojournal_volume
551pub_type
杂志文章相关文献
BRAIN RESEARCH文献大全abstract::We have previously shown that blockade of neurotransmission mediated by gamma-aminobutyric acid (GABA) in the ventromedial nucleus of the hypothalamus (VMH) by the microinjection of a GABAA receptor antagonist, bicuculline methiodide (BM), exclusively induced running activity in the rat. The purpose of the present stu...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(93)91243-l
更新日期:1993-02-19 00:00:00
abstract::The locus coeruleus was stimulated in 62 cats in order to investigate the effect on cephalic blood flow and cephalic vascular resistance. Flow was measured by electromagnetic flow probes applied to the common carotid artery. Stimulation over a range of frequencies (0.2-200 s-1) produced a frequency-dependent fall in c...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(83)90236-6
更新日期:1983-11-14 00:00:00
abstract::The mitochondrial respiratory chain is a powerful source of reactive oxygen species (ROS) also in noise induced hearing loss (NIHL) and anti-oxidants and free-radicals scavengers have been shown to attenuate the damage. Coenzyme Q(10) (CoQ(10)) or ubiquinone has a bioenergetic role as a component of the mithocondrial ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2008.12.027
更新日期:2009-02-27 00:00:00
abstract::The present study was designed to assess the effect of protein kinase C (PKC) and cAMP-dependent protein kinase (PKA) on desensitization of Torpedo acetylcholine (ACh) receptors by analyzing summated macroscopic currents in an outside-out patch-clamp configuration. Normal ACh receptors desensitized with a fast (6 ms) ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(98)00836-1
更新日期:1998-11-23 00:00:00
abstract::Neurotrophins are potent regulators of the survival of different neuronal populations in the CNS. Little is known of the immunodistribution of neurotrophin-3 (NT-3) and tyrosine kinase C (TrkC) receptor in the frog visual system, which can successfully regenerate and recover vision after injury. In this study we show ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(03)02948-2
更新日期:2003-08-22 00:00:00
abstract::To investigate whether differences in vulnerability to free radicals might underlie differences among striatal neurons in their vulnerability to neurodegenerative processes such as occur in ischemia and Huntington's disease, we have analyzed the localization of superoxide free radical scavengers in different striatal ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(95)01320-2
更新日期:1996-02-05 00:00:00
abstract::Neurodegenerative diseases, such as Alzheimer's disease (AD) and Parkinson's disease (PD), are characterized by progressive loss of cognitive function, dementia, and problems with movements. In order to find new protein biomarkers of high specificity from cerebrospinal fluid (CSF) of AD and PD patients, one-dimensiona...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2009.01.058
更新日期:2009-04-10 00:00:00
abstract::A common feature of many neurodegenerative disorders is an abundance of activated glial cells (astrocytes and microglia). In Alzheimer's disease (AD), activated astrocytes are in close apposition to and surrounding the amyloid plaques. The mechanisms by which the astrocytes become activated in AD and the consequences ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(97)01318-8
更新日期:1998-03-02 00:00:00
abstract::In a previous study, we have demonstrated that an ovalbumin-like antigen is present within approximately one-half of all neurons of chicken spinal ganglia. The current study demonstrates this antigen co-localizes absolutely with neural intermediate filament protein (Peripherin) in small to medium-sized neurons of spin...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(93)90342-k
更新日期:1993-11-12 00:00:00
abstract::To examine the effect of short-term and long-term adrenalectomy (ADX) on the glucocorticoid receptor (GR) expression, we performed an immunohistochemical study on the rat forebrain. One day after ADX, the GR-immunoreactivity significantly decreased or disappeared in most forebrain structures, while relatively strong G...
journal_title:Brain research
pub_type: 杂志文章
doi:
更新日期:1996-08-12 00:00:00
abstract::Spreading depression (SD) is characterized by a transient breakdown of neuronal function concomitant with a massive failure of ion homeostasis. It is a phenomenon that can be induced in neocortical tissue by raising excitability, e.g. injection of K(+), application of glutamatergic agonists, or blocking Na(+)/K(+) ATP...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(03)02600-3
更新日期:2003-06-13 00:00:00
abstract::The effect of repetitive stimulation of the locus coeruleus (LC) on the discharge rate of spontaneously active neurons of the visual, rostral and cingulate cortex was investigated in untreated and catecholamine-depleted rats under chloral hydrate anesthesia. In untreated animals, the inhibitory transsynaptic effects p...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(80)90251-6
更新日期:1980-03-17 00:00:00
abstract::The interposed nuclei (IN) of the intermediate cerebellum are critical components of the circuits that control associative learning of eyeblinks and other defensive reflexes in mammals. The IN, which represent the sole output of the intermediate cerebellum, receive massive GABAergic input from Purkinje cells of the ce...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2009.07.053
更新日期:2009-10-06 00:00:00
abstract::Fetal mouse retinal ganglion cell axons have been shown to ramify within co-cultured basement membrane secreting tumor explants and upon isolated basement membranes. Here we report that laminin, a glycoprotein found in basement membranes and adhesion sites of a variety of cell types, acted as a substrate for retinal e...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0165-3806(84)90184-6
更新日期:1984-01-01 00:00:00
abstract::The projections of putative dopamine containing cells within the dorsal raphe nucleus (DR) were studied using a combination of tyrosine hydroxylase (TH) immunocytochemistry and fluorescent retrograde tracing. Substantial numbers of TH-immunoreactive cells in the DR were found to project to the nucleus accumbens. Progr...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(90)90239-8
更新日期:1990-03-12 00:00:00
abstract::The second phase of Ca2+ transients (aequorin luminescence) which was not accompanied by twitch tensions were generated by 0.3 microM neostigmine in indirectly-stimulated diaphragm muscles of mice. This phase was dependent on external [Ca2+]o, was inhibited by a low concentration of (+)-tubocurarine (+)-TC; 0.013-0.13...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(90)90287-l
更新日期:1990-01-22 00:00:00
abstract:BACKGROUND:Empathizing is a specific component of social cognition. Empathizing is also specifically impaired in autism spectrum condition (ASC). These are two dimensions, measurable using the Empathy Quotient (EQ) and the Autism Spectrum Quotient (AQ). ASC also involves strong systemizing, a dimension measured using t...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2006.01.012
更新日期:2006-03-24 00:00:00
abstract::Combined amylin+leptin (AMN+LEP) can reduce diet induced obesity and is very effective in combating LEP resistance. The purpose of this study was to evaluate the effect of AMN+LEP on central histaminergic signaling in lean and obese rats. Male rats were administered LEP (300 μg/kg/d), AMN (100 μg/kg/d), AMN+LEP or veh...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2011.12.022
更新日期:2012-03-09 00:00:00
abstract::Recent studies stressed the importance of comparing exemplars both for improving performance by artificial classifiers as well as for explaining human category-learning strategies. In this report we provide a theoretical analysis for the usability of exemplar comparison for category-learning. We distinguish between tw...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2008.04.079
更新日期:2008-08-15 00:00:00
abstract::Seventy-nine single units were recorded with glass micropipettes applied onto taste pores of the anterior portion of the tongue in anesthetized hamsters. The receptive field of each recorded unit was located by iontophoretic stimulation applying either anodal or cathodal current (1-20 microA) to a non-stimulating solu...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(90)90643-P
更新日期:1990-04-02 00:00:00
abstract::Amyloid beta-protein (A beta) is the major protein of cerebrovascular and plaque amyloid in Alzheimer's disease (AD). Extensive evidence has demonstrated abnormal protein phosphorylation in this disease. We investigated the effect of synthetic A beta with the amino-acid sequence corresponding to cerebrovascular A beta...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(93)90479-7
更新日期:1993-11-26 00:00:00
abstract::Extracellular recordings from the supraoptic nucleus of the rat established that vasopressinergic neurosecretory cells were excited by stimulation of cervical but not abdominal vagal afferents. This response was absent or significantly attenuated after microinjection of gamma-aminobutyric acid into a region of the cau...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(92)91131-w
更新日期:1992-10-30 00:00:00
abstract::The release of [3H]acetylcholine (ACh) from 6-, 8-, 10-, 12-, 14- and 16-day chick embryo neural retina layers was determined using a perfusion system to collect the released neurotransmitter. After labeling the tissue with [3H]choline, samples of [3H]ACh released before and after stimulation by 80 mM KCl or 5 mM glut...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0165-3806(82)90137-7
更新日期:1982-07-01 00:00:00
abstract::Audiogenic kindling (AK) represents a model of naturally occurring epileptogenesis in which intensification of repeatedly induced audiogenic seizures results from propagation of epileptic activity from the brainstem to forebrain. Previously it has been shown that unilateral cortical spreading depression (SD) is a reli...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2010.03.037
更新日期:2010-05-06 00:00:00
abstract::We have developed a RNA-RNA solution hybridization assay to quantitate the mRNA coding for prodynorphin precursor. This assay is extremely sensitive and highly specific. Using this assay we have measured the prodynorphin mRNA in various brain regions and reproductive tissues of rat. When we compared the distribution o...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(87)80011-2
更新日期:1987-07-01 00:00:00
abstract::Cultures of mouse spinal cord were used to visualize binding sites for [125I]angiotensin II (AII) by autoradiography. Visualization by light microscopy shows that neurones, but also glial cells possess angiotensin II binding sites which are located both on soma and processes. These findings open a new field of investi...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(88)91642-3
更新日期:1988-03-08 00:00:00
abstract::The aim of this study was to identify the medullary vagal nuclei involved in the different phases of swallowing activated physiologically in a species with an esophagus similar to human. In decerebrate cats, the pharyngeal (0.5-1.0 ml water in pharynx (N=6)) or esophageal (1-3 ml air in esophagus (N=5)) phases of swal...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2004.03.061
更新日期:2004-07-16 00:00:00
abstract::Corticotropin-releasing factor (CRF) has been hypothesized to be involved in the pathophysiology of anxiety, depression, cognitive and feeding disorders. Two distinct CRF receptor subtypes, CRFR1 and CRFR2, are thought to mediate CRF actions in the CNS. However, the role for each receptor subtype in animal models of n...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(98)01158-5
更新日期:1999-07-17 00:00:00
abstract::In order to study the biosynthesis of myelin-associated proteins in Schwann cells, we have induced proliferation of cultured Schwann cells from neonatal rat sciatic nerve by transformation with Simian Virus 40 (SV40). Homogeneous transformed Schwann cell lines were established by single cell cloning. The transformed p...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(87)91324-2
更新日期:1987-06-23 00:00:00
abstract::Depending on experimental conditions, chronic cocaine exposure can induce an increase in binding to the dopamine transporter (DAT). One possible mechanism for the cocaine-induced-upregulation in DAT binding sites is through stimulation of presynaptic D2 receptors by excess synaptic dopamine. To test this hypothesis, t...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(96)01033-5
更新日期:1996-12-02 00:00:00