Abstract：
:The present study was designed to assess the effect of protein kinase C (PKC) and cAMP-dependent protein kinase (PKA) on desensitization of Torpedo acetylcholine (ACh) receptors by analyzing summated macroscopic currents in an outside-out patch-clamp configuration. Normal ACh receptors desensitized with a fast (6 ms) and slow time constant (104 ms). There was no significant difference in the current decay time between normal ACh receptors and mutant ACh receptors that possibly mimics PKC phosphorylation of the receptors. The selective PKC inhibitor, PKCl, prolonged the rate of desensitization of normal ACh receptors, and the similar effect was obtained with mutant ACh receptors lacking PKC phosphorylation sites. Phosphorylation of normal ACh receptors by the catalytic subunit of PKA or mutant ACh receptors that possibly mimic PKA phosphorylation of the receptors increased the rate of desensitization, but, in contrast, the receptors lacking PKA phosphorylation sites prolonged the current decay time. The results of the present study demonstrate that PKC or PKA phosphorylation of ACh receptors accelerates the rate of desensitization.
journal_name
Brain Resjournal_title
Brain researchauthors
Nishizaki T,Sumikawa Kdoi
10.1016/s0006-8993(98)00836-1subject
Has Abstractpub_date
1998-11-23 00:00:00pages
242-5issue
1-2eissn
0006-8993issn
1872-6240pii
S0006-8993(98)00836-1journal_volume
812pub_type
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