Abstract：
:Amyloid beta-protein (A beta) is the major protein of cerebrovascular and plaque amyloid in Alzheimer's disease (AD). Extensive evidence has demonstrated abnormal protein phosphorylation in this disease. We investigated the effect of synthetic A beta with the amino-acid sequence corresponding to cerebrovascular A beta and plaque A beta on the activities of casein kinase I (CK I) and casein kinase II (CK II). These enzymes were purified from bovine brain and casein was used as a substrate. A beta was found to stimulate markedly CK I- and CK II-mediated phosphorylation of casein in a concentration-dependent manner. The effect of plaque A beta was considerably higher than that of cerebrovascular A beta. Heparin, which is known to be a specific inhibitor of CK II, completely inhibited A beta-stimulated CK II activity. A beta itself was not a substrate for casein kinases. These findings were confirmed using other substrates for CK I and CK II. The experiments with synthetic CK II-substrate peptide (Leu-Glu-Leu-Ser-Asp-Asp-Asp-Asp-Glu) and the phosphorylation of erythrocyte membrane proteins by intrinsic membrane-bound CK I in erythrocytes showed marked stimulation in activities of casein kinases in the presence of A beta 1-40 or blocked A beta. We propose that A beta, by stimulating casein kinases, may contribute to abnormal protein phosphorylation in AD, in particular to increased phosphorylation of microtubule-associated proteins, leading to the neurofibrillary tangles formation and neurodegeneration in this disease. Interaction of A beta with protein kinases, thus, may characterize the beginning of the disease.
journal_name
Brain Resjournal_title
Brain researchauthors
Chauhan A,Chauhan VP,Murakami N,Brockerhoff H,Wisniewski HMdoi
10.1016/0006-8993(93)90479-7subject
Has Abstractpub_date
1993-11-26 00:00:00pages
47-52issue
1eissn
0006-8993issn
1872-6240pii
0006-8993(93)90479-7journal_volume
629pub_type
杂志文章相关文献
BRAIN RESEARCH文献大全abstract：:The aim of this study was to examine the involvement of the hypothalamic oxytocin (OXT) and vasopressin (AVP) neurons in acute phase reaction using quantitative dual-labeled immunostaining with Fos and either OXT and AVP in several hypothalamic regions. Administration of low dose (5 microg/kg) and high dose (125 micro...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(99)02418-x
更新日期：2000-03-06 00:00:00
abstract：:We sought to further elucidate signal transduction pathways for the I(1)-imidazoline receptor in PC12 cells and their interaction with the well-characterized signaling events triggered by nerve growth factor (NGF) in these cells. Stimulation of the I(1)-imidazoline receptor with moxonidine, a centrally acting antihype...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(03)02893-2
更新日期：2003-08-01 00:00:00
abstract：:Our recent study has shown that many afferent fibers in the ventral root are third branches of dorsal root ganglion cells in addition to their processes in the peripheral nerve and the dorsal root. From results of this study, we hypothesized that most of the afferent fibers in the normal ventral root are extra process...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(89)91030-5
更新日期：1989-10-30 00:00:00
abstract：:In order to map the recently cloned serotonin 5-HT6 receptor in the rat brain and spinal cord, polyclonal antibodies were raised against a synthetic octadecapeptide corresponding to a specific portion (Leu398-Val415) of the C-terminal domain of this receptor. Antibodies were detected by enzyme-linked immunosorbent ass...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(96)01224-3
更新日期：1997-01-23 00:00:00
abstract：:This study examined the projection from the rostral nucleus of the solitary tract (rNST) to the medial parabrachial nucleus (mPBN) in male Wistar rats using DiI as a retrograde tracer and biotinylated dextran as an anterograde tracer. Following successful unilateral injection of DiI into the mPBN (n = 8), retrogradely...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(96)00736-6
更新日期：1996-10-21 00:00:00
abstract：:The parabrachial nucleus consists of several subnuclei which contains autonomic, gustatory, visceral sensory, nociceptive, and respiratory related neurons. We have investigated the direct projections from the rat parabrachial region, including the K olliker-Fuse nucleus, to the pharyngeal motoneurons with an anterogra...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(98)01127-5
更新日期：1999-01-23 00:00:00
abstract：:Acute and repeated exposure to cocaine induces long-lasting alterations in neural networks that underlie compulsive drug seeking and taking. Cocaine exposure triggers complex adaptations in the brain that are mediated by dynamic patterns of gene expression that are translated into enduring changes. Recently, epigeneti...
journal_title：Brain research
pub_type： 杂志文章,评审
doi：10.1016/j.brainres.2014.09.069
更新日期：2015-12-02 00:00:00
abstract：:The analgesia induced by 30-s footshock (1 mA) is enhanced and attenuated by decreasing and increasing the extraneuronal availability of serotonin (5-HT), respectively. In the present work we have shown the effect to be mediated by spinal 5-HT systems as the response was increased by depletion of spinal but not brain ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(82)90448-6
更新日期：1982-04-15 00:00:00
abstract：:The dependence of the carotid chemosensory response to hypoxia on metabolic substrate and the hypothesis that lactic acidosis is essential for O2 chemoreception were tested. Effects of 3 types of substrate (glucose, glutamate and a mixture of amino acids) on the response to hypoxia (perfusate flow interruption) were m...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(92)91535-m
更新日期：1992-11-20 00:00:00
abstract：:The intracellular injection technique of HRP with simultaneous recordings of intracellular potential was applied to the crab X-organ-sinus gland peptidergic neurosecretory neurons. At least two classes of neurons were discriminated from the usual type of neurosecretory neurons morphologically as well as electrophysiol...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(86)90468-3
更新日期：1986-01-08 00:00:00
abstract：:Fluorescence immunohistochemistry was performed to characterize the distribution and phenotype of GLUT8-positive neurons in rat brain and to compare the cellular distribution of GLUT8 with GLUT3 in the hippocampus. Based upon the absence of co-localization with the non-neuronal markers GFAP (astroglial) and OX42 (micr...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(02)02308-9
更新日期：2002-04-05 00:00:00
abstract：:On the day of birth, female rats received either a thoraco-lumbar spinal transection or sham operation, followed by administration of either testosterone propionate (TP) or oil immediately after surgery and again on the third day of life. Upon sacrifice at 30 days of age examination of spinal cords revealed that TP ma...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0165-3806(85)90047-1
更新日期：1985-12-01 00:00:00
abstract：:Variables influencing decision-making in real settings, as in the case of voting decisions, are uncontrollable and in many times even unknown to the experimenter. In this case, the experimenter has to study the intention to decide (vote) as close as possible in time to the moment of the real decision (election day). H...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2010.06.046
更新日期：2010-09-10 00:00:00
abstract：:We previously reported that the level of Ca2+/calmodulin-dependent protein kinase II (CaM kinase II) alpha and beta proteins increases with postnatal age. In the present study, we investigated the developmental changes in whole protein substrates of CaM kinase II as compared with those of cAMP-dependent protein kinase...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(94)90861-3
更新日期：1994-10-03 00:00:00
abstract：:We previously reported the presence of Erk2 type mitogen-activated protein kinase (MAPK) and enrichment of its substrates in the post-synaptic density (PSD) fraction, and suggested a role for MAPK in the synaptic transmission and its modulation [Suzuki, T., Okumura-Noji, K., Nishida, E., ERK2-type mitogen-activated pr...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(99)01762-x
更新日期：1999-09-04 00:00:00
abstract：:Parkinson's disease (PD) is a common neurodegenerative disease characterized by progressive loss of midbrain dopaminergic neurons. To gain an insight into the mechanisms underlying the progression of PD, gene expression analysis was performed using two different brain regions, the substantia nigra pars compacta (SN) a...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2010.05.066
更新日期：2010-07-30 00:00:00
abstract：INTRODUCTION:Ischemic postconditioning (IpostC) has been described in both heart and brain. The first aim of this study was to evaluate the effects of IpostC on brain infarct size and neurological function in the middle cerebral artery occlusion (MCAO) model. The second aim was to determine the involvement of the mitoc...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2010.12.054
更新日期：2011-02-23 00:00:00
abstract：:The amygdala plays an important role in the regulation of motivational states, especially those associated with addiction. The amygdala also expresses high levels of brain-derived neurotrophic factor (BDNF), an activity-dependent neurotrophin that can influence the reinforcing and locomotor activating properties of ps...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(02)03160-8
更新日期：2002-09-13 00:00:00
abstract：:Calcium accumulation and neuronal injury of rat cortical cell cultures in vitro were examined following oxygen deprivation under conditions of normal and low glucose. 45Ca2+ uptake and lactate dehydrogenase (LDH) release, measured at 12 and 24 h after oxygen deprivation, were significantly elevated in cultures exposed...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(93)91527-y
更新日期：1993-04-02 00:00:00
abstract：:Rapid eye movement (REM) sleep is hypothesized to result from the activity of REM sleep-generating and REM sleep-inhibiting neurons. The serotoninergic (5-HT) neurons of the dorsal raphe nucleus (DRN) represents one such population of REM-sleep inhibiting neurons since they are silent during REM sleep. Consistent with...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(01)03209-7
更新日期：2001-12-27 00:00:00
abstract：:The results of a WGA-HRP and HRP study in the cat indicated that some neurons in the marginal zone (lamina I) of the caudal spinal trigeminal nucleus sent their axons contralaterally to the striatum; mainly to the dorsal part of the putamen, and additionally to the ventrolateral part of the caudate nucleus, at the ste...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(87)90400-8
更新日期：1987-04-07 00:00:00
abstract：:A study of seizure activity and neuronal cell death produced by intracerebroventricular kainic acid had suggested that seizures conveyed by the hippocampal mossy fibers are more damaging to CA3 pyramidal cells than seizures conveyed by other pathways. To test this idea, the effects of a unilateral mossy fiber lesion w...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(90)91171-c
更新日期：1990-03-26 00:00:00
abstract：:Autoradiographic localization of binding sites for [3H]prostaglandin D2, E2 and F2 alpha in the monkey brain was investigated by using in vitro labeling and an image processing system. Specific binding sites were distinctly localized in various nuclei in the hypothalamus, thalamus, and limbic system and their localiza...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(89)91486-8
更新日期：1989-01-23 00:00:00
abstract：:Dentate granule cells can be selectively destroyed by intrahippocampal injections of colchicine. This study evaluates the consequences of granule cell destruction on blood pressure regulation in the normotensive Wistar-Kyoto (WKY) and spontaneously hypertensive rat (SHR). Bilateral destruction of dentate granule cells...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(94)91473-7
更新日期：1994-09-12 00:00:00
abstract：:The nicotine and muscarinic responses of differentiated mouse neuroblastoma cells from the clonal line N1E 115 to applied cholinergic agents were recorded using single channel and whole cell patch clamp techniques. An inward macroscopic current induced by acetylcholine (ACh) at the resting potential was blocked by cur...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(86)91252-7
更新日期：1986-11-19 00:00:00
abstract：:The distribution of substance P (SP) immunoreactivity in the spinal nucleus of the rat trigeminal nerve and in the skin of the lower lip was examined following (a) unilateral electrolytic lesions of the trigeminal ganglion, (b) trigeminal rhizotomy, and (c) unilateral interruption of the mental nerve, the sensory bran...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(78)91105-8
更新日期：1978-09-08 00:00:00
abstract：:Following the administration of horseradish peroxidase (HRP) in the middle or lower cervical cord segments of neonatal (1-10 days), developing (15-20 days) and mature (2-4 months) albino rats, labeled pyramidal neurons were found in layer V of the cerebral cortex. They formed one continuous band of labeled neurons in ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(83)91327-6
更新日期：1983-04-11 00:00:00
abstract：:The overall volume of the three main inferior olivary (ION) subnuclei increases four-fold between the day of birth (PO) and P21. The rate of this increase is uneven; between P10 and P15 there is an abrupt acceleration which parallels the period of intense synaptogenesis and maturation of the neuropil. The growth of th...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0165-3806(84)90029-4
更新日期：1984-11-01 00:00:00
abstract：:Reactive oxygen species (ROS) are supposed to be involved in neurodegenerative processes like Parkinson's or Alzheimer's disease. Beside this there are an increasing number of studies indicating an involvement of ROS in traumatic brain injury. We therefore studied the potential role of astrocytes against neurotoxic ef...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(96)01029-3
更新日期：1996-12-16 00:00:00
abstract：:The main purpose of the present study was to determine whether specific lymphokines may be neurotrophic, by testing their effects on the survival of hippocampal neuronal cultures. Previous studies have shown that a variety of interleukins may be neurotrophic or neurotoxic, depending upon the culture conditions, as wel...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(93)90400-h
更新日期：1993-01-08 00:00:00
