Abstract：
:We sought to further elucidate signal transduction pathways for the I(1)-imidazoline receptor in PC12 cells and their interaction with the well-characterized signaling events triggered by nerve growth factor (NGF) in these cells. Stimulation of the I(1)-imidazoline receptor with moxonidine, a centrally acting antihypertensive, increased by greater than two-fold the proportion of ERK-1 and ERK-2 in the phosphorylated active form. Similarly, NGF elicited a five-fold increase in activated ERKs. Surprisingly, treatment of NGF-treated cells with moxonidine completely reversed activation of ERK. Moxonidine-induced inhibition of ERK activation in NGF-treated cells was dose-dependent, followed a limited time course and could be blocked by the I(1)-antagonist efaroxan. These data suggested possible deactivation of ERK by specific phosphatases. Therefore, we assayed levels of MKP-2, a dual specificity phosphatase whose substrates include ERK. Moxonidine and NGF both increased levels of MKP-2 by three-fold. These effects were additive, as both agents together increased MKP-2 by a total of six-fold. Moxonidine-induced induction of MKP-2 was time- and dose-dependent and could be blocked by the I(1)-antagonist efaroxan or by D609, an inhibitor of phosphatidylcholine-selective phospholipase C known to block downstream signaling events coupled to I(1)-receptors. Thus, I(1)-receptors can abrogate the primary signaling cascade activated by NGF, most likely by increasing levels of a specific phosphatase to return dually phosphorylated ERK to its unphosphorylated state.
journal_name
Brain Resjournal_title
Brain researchauthors
Edwards L,Ernsberger Pdoi
10.1016/s0006-8993(03)02893-2subject
Has Abstractpub_date
2003-08-01 00:00:00pages
71-9issue
1eissn
0006-8993issn
1872-6240pii
S0006899303028932journal_volume
980pub_type
杂志文章相关文献
BRAIN RESEARCH文献大全abstract：:Rats exposed to stressors that cannot be controlled may develop a deficit in their ability to subsequently learn to control a new stressor. This phenomenon is known as 'learned helplessness' and is a well-accepted animal model of depression. Evidence is presented showing that rats having different directional biases o...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(91)91335-x
更新日期：1991-06-07 00:00:00
abstract：:Propofol (2,6-diisopropylphenol) is an intravenous general anaesthetic which can directly activate and positively modulate the GABAA receptor. The effects of propofol on human recombinant GABAA receptors were studied in Xenopus oocytes expressing either alpha1beta2, alpha1beta2gamma2L, or alpha2beta2gamma2L receptor i...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(97)01334-6
更新日期：1998-02-16 00:00:00
abstract：:Membrane properties of rat magnocellular neuroendocrine cells (MNCs) were examined during intracellular recordings in vivo. Current-clamp experiments revealed sustained outward rectification positive to -55 mV and transient outward rectification of depolarizing responses elicited from negative holding potentials. Trai...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(91)90535-4
更新日期：1991-02-01 00:00:00
abstract：:Physical exercise can induce brain plasticity and reduce the cognitive decline observed in type 1 diabetes mellitus (T1DM). We investigated the effects of physical exercise to prevent or reverse spatial memory deficits produced by diabetes and some biochemical and immunohistochemical changes in hippocampal astrocytes ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2016.10.024
更新日期：2017-01-15 00:00:00
abstract：:Levodopa (L-DOPA) is widely used for symptomatic management in Parkinson's disease. We recently showed that (-)-epigallocatechin-3-gallate, a tea polyphenol, not only inhibits L-DOPA methylation, but also protects against oxidative hippocampal neurodegeneration. In the present study, we sought to determine several oth...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2012.11.043
更新日期：2013-02-25 00:00:00
abstract：:The present study investigated the hypothesis that social isolation increases aggression by increasing the number of V1a vasopressin receptors in the anterior hypothalamus (AH). Male hamsters were randomly assigned to a group that was allowed to interact with a small nonaggressive hamster three times each week for 3 w...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2005.12.081
更新日期：2006-02-16 00:00:00
abstract：:N-stearoyltyrosine (NsTyr), a synthesized anandamide (AEA) analogue, was evaluated for the first time in the present study for the neuroprotective effect in gerbils subjected to transient global cerebral ischemia reperfusion (IR). The extent of ischemia injury was assessed behaviorally by measuring neurological functi...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2009.06.070
更新日期：2009-09-01 00:00:00
abstract：:The action of proline and its analog as glutamate antagonists was investigated in CNS tissue of the neonatal chick. Avian brain slices were incubated in low concentrations of L-proline, D-proline, DL-3,4-dehydro-proline, L-prolyl-L-proline, or in avian physiological salt solution before depolarization was induced by a...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0165-3806(83)90056-1
更新日期：1983-08-01 00:00:00
abstract：:Severed medial giant axons in crayfish can be rejoined in vitro with polyethylene glycol (PEG) to produce axoplasmic continuity and through transmission of action potentials. Severed axon-like processes of a mammalian neuroblastoma/glioma cell line seem to be rejoined to the cell body using PEG in tissue culture. Our ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(86)91617-3
更新日期：1986-03-05 00:00:00
abstract：:This study evaluated the hypothesis that the dorsal motor nucleus (DMN) of the brainstem may mediate the ulcerogenic and acid-stimulatory effects of thyrotropin-releasing hormone (TRH) in rats. To accomplish this, intra-DMN microinjections of TRH (50 and 500 ng) were performed and their effects on acid secretion and g...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(88)90295-8
更新日期：1988-08-30 00:00:00
abstract：:Male Wistar rats were anaesthetized, injected intracisternally (i.c.) with saline or colchicine and were decapitated at various time intervals. Trunk blood was collected for the determination of immunoreactive adrenocorticotropic hormone (ACTHi) by radioimmunoassay (RIA) and of corticosterone by a fluorometric assay. ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(88)91517-x
更新日期：1988-03-01 00:00:00
abstract：:Structures of the medial temporal lobes are recognized to play a central role in memory processing and to be the primary sites of deterioration in Alzheimer disease (AD). Mild cognitive impairment (MCI) represents potentially an intermediate state between normal aging and AD. Proton magnetic resonance spectroscopy (MR...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2003.11.074
更新日期：2004-04-02 00:00:00
abstract：:A histochemical stain for cytochrome oxidase (CO) activity was used to examine the maturation of a neurochemical correlate of ON and OFF channels in the retina and dorsal lateral geniculate nucleus (LGN) of the tree shrew. In the adult tree shrew, the CO staining pattern can be used as a histochemical marker of segreg...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0165-3806(87)90217-3
更新日期：1987-08-01 00:00:00
abstract：:Coated vesicles within dentate gyrus granule cell dendrites were found free in the cytoplasm and also attached to the smooth endoplasmic reticulum and plasmalemma. Coated vesicles began increasing in number 40-48 h following partial dendritic deafferentation. This increase continued up to 15-30 days postlesion, after ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(81)90076-7
更新日期：1981-04-27 00:00:00
abstract：:After a moderate global cerebral ischemia, two hypothetical populations of pyramidal neurons are present among the hippocampal CA1 pyramidal neurons: one that will die and another one that will survive. Prior analysis of dissected hippocampal CA1 regions has shown a reduction of the GluR1-3 mRNA following ischemia. In...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(01)01985-0
更新日期：2001-03-09 00:00:00
abstract：:Rat striata were exposed to 15 mM quinolinic acid (QUIN), or QUIN plus the nitric oxide synthase inhibitors S-methyl-L-thiocitrulline dihydrochloride (L-MIN) or 7-nitroindazole monosodium salt (7-NINA) for 21 days. Co-administration of 100 microM or 1 mM L-MIN with QUIN significantly reduced lesion volume compared to ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(97)00962-1
更新日期：1997-11-14 00:00:00
abstract：:How does attention interact with learning? Kruschke [Kruschke, J.K. (2001). Toward a unified Model of Attention in Associative Learning. J. Math. Psychol. 45, 812-863.] proposed a model (EXIT) that captures Mackintosh's [Mackintosh, N.J. (1975). A theory of attention: Variations in the associability of stimuli with re...
journal_title：Brain research
pub_type： 杂志文章,评审
doi：10.1016/j.brainres.2007.06.097
更新日期：2008-04-02 00:00:00
abstract：:Previous work, using membrane receptor binding techniques, demonstrated an increase in hippocampal MK-801 binding sites in mice after chronic ethanol ingestion. The current studies, using quantitative autoradiography, demonstrate that chronic ethanol ingestion also produces increases in MK-801 binding in cerebral cort...
journal_title：Brain research
pub_type： 杂志文章
doi：
更新日期：1991-04-26 00:00:00
abstract：:The trace elemental concentrations, including iron, in the substantia nigra (SN) of a 6-OHDA induced rat model of Parkinson's disease were measured using nuclear microscopy. Only rats that exhibited amphetamine induced rotation of more than 7 turns/min were used. The results showed that the iron levels were significan...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(96)00313-7
更新日期：1996-09-30 00:00:00
abstract：:The anteroventral subdivision of the medial amygdala (MeAV) is one of the vomeronasal structures involved in the control of hormonally dependent behaviors such as sexual and agonistic behaviors in rats. The present study investigates some anatomical and neurochemical parameters of this nucleus (volume, number of neuro...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2007.02.073
更新日期：2007-05-30 00:00:00
abstract：:The current study investigated the time course of the implicit processing of erotic stimuli using event-related potentials (ERPs). ERPs elicited by erotic pictures were compared with those by three other types of pictures: non-erotic positive, negative, and neutral pictures. We observed that erotic pictures evoked enh...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2012.10.019
更新日期：2012-12-13 00:00:00
abstract：:The present study investigates the effects of gammavinylgaba (GVG, 4 days treatment at 50 and 200 mg/kg), a gabatransaminase inhibitor, on the high-voltage spindle (HVS) activity in control and nucleus basalis-lesioned rats. GVG treatment at 200 mg/kg, but not at 50 mg/kg increased HVS activity in controls. GVG greatl...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(91)91667-p
更新日期：1991-11-29 00:00:00
abstract：:Maternal care is crucial for offspring development and licking/grooming patterns can be induced by sensorial, neuroendocrine, and metabolic variations in the CNS. Important brain functions, such as learning and memory, can be influenced by oxidative stress, which can also modulate pathophysiological processes (e.g., d...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2016.11.010
更新日期：2017-01-15 00:00:00
abstract：:Depolarizing actions of 4 conformationally restricted L-glutamate analogues, (2S,3S,4S) isomer (L-CCG-I), (2S,3R,4R) isomer (L-CCG-II), (2S,3S,4R) isomer (L-CCG-III) and (2S,3R,4S) isomer (L-CCG-IV) of L-alpha-(carboxycyclopropyl)-glycine (L-CCG), were investigated in the isolated rat spinal cord by extracellular reco...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(89)90207-2
更新日期：1989-02-20 00:00:00
abstract：:Pain resulting from peripheral nerve injury, characterised by ongoing pain, hyperalgesia and allodynia arises from peripheral and central processes. Here, we studied the potential role of central facilitations in nerve injury by investigating the effect of blocking the excitatory 5HT3 receptor with ondansetron. 5HT3 r...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2004.05.108
更新日期：2004-09-03 00:00:00
abstract：:The relationship between the dorsal raphe neurons projecting to the caudate-putamen and those projecting to the substantia nigra was investigated using a fluorescent retrograde double labeling technique in the rat. The majority of the dorsal raphe neurons projecting to the substantia nigra were found to project also t...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(80)90250-4
更新日期：1980-03-17 00:00:00
abstract：:Axoaxonic synapses are common features of hair-follicle afferent boutons. We have attempted to discover the identity of the neurotransmitter in the associated axon terminals by combining intra-axonal labelling of hair-follicle afferent axons with immunocytochemistry in a correlated light and electron microscopical stu...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(87)90394-5
更新日期：1987-04-07 00:00:00
abstract：:Following infection with influenza virus, animals display decreased locomotor activity and feeding behavior and loss of body weight. It has been suggested that these effects may be mediated by cytokines, such as interleukin-1 (IL-1), interleukin-6 (IL-6) and tumor necrosis factor alpha (TNF-alpha), induced by the infe...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(97)01009-3
更新日期：1997-11-21 00:00:00
abstract：:The subfornical organ (SFO), a neuroendocrine structure implicated in saltwater homeostasis, contains secretory structures histochemically similar to those in the neurohypophysis. Because of these morphological similarities, we compared levels of vasopressin (vp) in the SFO area and the adjacent hippocampal commissure...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(78)90458-4
更新日期：1978-01-27 00:00:00
abstract：:Projections from the hypothalamus to cells of origin of the greater petrosal nerve were studied by the HRP and autoradiographic techniques in the rat. After an injection of [3H]amino acids into the hypothalamus and an application of HRP to the greater petrosal nerve, we found that a compact group of HRP-labeled neuron...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(84)90744-3
更新日期：1984-01-02 00:00:00
