Effect of axonal transport blockade on corticotropin-releasing factor immunoreactivity in the median eminence of intact and adrenalectomized rats: relationship between depletion rate and secretory activity.


:Male Wistar rats were anaesthetized, injected intracisternally (i.c.) with saline or colchicine and were decapitated at various time intervals. Trunk blood was collected for the determination of immunoreactive adrenocorticotropic hormone (ACTHi) by radioimmunoassay (RIA) and of corticosterone by a fluorometric assay. Changes in corticotropin-releasing factor (CRF) content of the median eminence (ME) were assessed by quantitative immunocytochemistry (QICC) on cryostat sections of ME preparations or by RIA of CRF in ME-extracts. Administration of colchicine resulted in a long-lasting and dose-dependent stimulation of ACTHi secretion. At a dose of 25 micrograms, high plasma ACTHi levels were found for up to 24 h. A dose of 5 micrograms per rat, that has been reported to effectively block vasopressin transport in paraventricular-neurohypophyseal neurons, resulted in a small elevation of plasma ACTHi. In intact rats, i.c. administration of saline of saline containing 5 micrograms of colchicine had no effect on the CRF content in the ME. In contrast, colchicine caused a depletion of the CRF stores in the ME of 1-week adrenalectomized (ADX) rats. The disappearance rate was 9.2%/h as measured by RIA and 11.2%/h as measured by QICC. When plasma corticosterone and ACTHi were normalized by giving ADX rats corticosterone in drinking water, the colchicine-induced depletion of the CRF stores was fully prevented. We conclude that the rate of decline of CRF in the ME of rats treated with a non-toxic dose of colchicine to block axonal transport is positively correlated to the secretory activity of CRF neurons of the paraventricular-infundibular system.


Brain Res


Brain research


Berkenbosch F,Tilders FJ




Has Abstract


1988-03-01 00:00:00














  • Catecholamine-containing neurons and lymphoid cells in a lacrimal gland of the pigeon.

    abstract::The Harderian gland of birds, a major lacrimal gland, contains a large population of lymphoid cells that produce IgA, a significant component of tears. Using histochemical techniques, we have examined the innervation of the gland. There was an extensive acetylcholine-esterase positive fiber network throughout the glan...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Walcott B,McLean JR

    更新日期:1985-02-25 00:00:00

  • Physiological properties of intradental mechanoreceptors.

    abstract::A major role of tooth receptors in signaling overt or impending tissue damage (nociception) has been previously established by substantial evidence from mechanical, thermal and chemical stimulation of exposed dentin. We report evidence showing that some intradental receptors in canine teeth of the cat detect mechanica...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Dong WK,Chudler EH,Martin RF

    更新日期:1985-05-20 00:00:00

  • Cerebrospinal fluid (CSF) and brain monoamine metabolites in the developing rat pup.

    abstract::Concentrations of the neurotransmitters, serotonin (5-HT), dopamine (DA), and norepinephrine (NE) were measured in the developing rat brain at 12, 19, 26 and 42 days of age. The amino acid precursors, tryptophan (TRP) and tyrosine (TYR) were measured along with the 5-HT and DA metabolites, 5-hydroxyindoleacetic acid (...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Shaywitz BA,Anderson GM,Cohen DJ

    更新日期:1985-01-01 00:00:00

  • Temporal patterns of the embryonic intermediate filaments nestin and vimentin expression in the cerebral cortex of adult rats after cryoinjury.

    abstract::The expression of two embryonic intermediate filaments, nestin and vimentin, in the rat brain at days 0 (control), 1, 4, 7 and 14 post-cryoinjury was studied to elucidate their roles in brain injury. Western blot analysis showed that both nestin and vimentin expressions in the ipsilateral cerebral cortex were signific...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Moon C,Ahn M,Kim S,Jin JK,Sim KB,Kim HM,Lee MY,Shin T

    更新日期:2004-12-03 00:00:00

  • Effects on the growth of damaged ganglion cell axons after peripheral nerve transplantation in adult hamsters.

    abstract::After transplantation of autologous sciatic nerve segments into the retina of adult hamsters for 1-2 months, retrograde labelling with horseradish peroxidase demonstrated a population of ganglion cells situated peripheral to the graft. If an additional lesion was placed between the insertion of the graft and the optic...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: So KF,Xiao YM,Diao YC

    更新日期:1986-07-02 00:00:00

  • Release of D-[3H]aspartate and [14C]GABA in rat hippocampus slices: effects of fatty acid-free bovine serum albumin and Ca2+ withdrawal.

    abstract::Extended incubation of hippocampus slices in the presence of fatty acid-free bovine serum albumin (FAF-BSA) strongly enhanced the release of D-[3H]aspartate and [14C]GABA induced by veratridine. Saturation of the FAF-BSA with oleic acid abolished the enhancing effect. Spontaneous release and K+-induced release were no...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Minc-Golomb D,Eimerl S,Levy Y,Schramm M

    更新日期:1988-08-09 00:00:00

  • Pattern of sensory innervation of the perineal skin in the female rat.

    abstract::Here we describe the nerves innervating the perineal skin together with their sensory fields in the adult female rat. Electrophysiological recording showed that the lumbosacral and L6-S1 trunks, in part by way of the sacral plexus, transmit sensory information from the perineal skin via four nerves: the viscerocutaneo...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Cruz Y,Zempoalteca R,Angelica Lucio R,Pacheco P,Hudson R,Martínez-Gómez M

    更新日期:2004-10-22 00:00:00

  • Retrograde axonal transport following injection of [3H]-serotonin into the olfactory bulb. II. Radioautographic study.

    abstract::Radioautography was used to study the intraneuronal distribution of [3H]-serotonin (5-HT) and/or its derivatives selectively taken up by the olfactory bulb (OB) serotonergic terminals and subsequently transported to their parent cell bodies in the midbrain raphe nuclei. This was done 24 h after injection of [3H]5-HT i...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Araneda S,Gamrani H,Font C,Calas A,Pujol JF,Bobillier P

    更新日期:1980-09-08 00:00:00

  • G-protein effects on retrograde axonal transport.

    abstract::Movements of medium and large sized membranous organelles (0.5-1 microns in diameter) were visualized within segments of the crab walking leg nerve with Nomarski differential interference contrast optics and subjected to video contrast enhancement. Accessibility to the axoplasm was demonstrated by intra-axonal fluores...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Moshiach S,Nelson TJ,Sanchez-Andres JV,Sakakibara M,Alkon DL

    更新日期:1993-03-12 00:00:00

  • Kinetics of potassium movement in norman cortex.

    abstract::Artificial CSF containing 12mEq/1 of potassium (4 times normal concentration) was perfused over the surface of normal neocortex in 10 locally or barbiturate anesthetized cats. Extracellular potassium concentrations ([K+]OS) were measured as a function of time to 8 h and of depth to 3 mm. Analysis of the [K+]O versus d...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Fisher RS,Pedley TA,Prince DA

    更新日期:1976-01-16 00:00:00

  • Glycine cleavage system in astrocytes.

    abstract::The localization of the glycine cleavage system was examined in the rat brain by immunohistochemistry using an antibody to P-protein (a constituent of the system). In all sites studied, the enzyme was confined to the astrocytes. The intensity of astrocyte staining varied in different brain regions, with the strongest ...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Sato K,Yoshida S,Fujiwara K,Tada K,Tohyama M

    更新日期:1991-12-13 00:00:00

  • Altered local cerebral glucose utilization induced by electrical stimulations of the thalamic sensory and parafascicular nuclei in rats.

    abstract::Alterations in local cerebral glucose utilization (LCGU) induced by electrical stimulation of the sensory relay nucleus (VPL) or parafascicular nucleus (Pf) of the thalamus in conscious rats were measured by the [14C]2-deoxyglucose method, the objective being to assess the mechanism of analgesia induced by electrical ...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Aiko Y,Shima F,Hosokawa S,Kato M,Kitamura K

    更新日期:1987-04-07 00:00:00

  • Some electrophysiological and pharmacological properties of the cortical, noradrenergic projection of the locus coeruleus in the rat.

    abstract::The effect of repetitive stimulation of the locus coeruleus (LC) on the discharge rate of spontaneously active neurons of the visual, rostral and cingulate cortex was investigated in untreated and catecholamine-depleted rats under chloral hydrate anesthesia. In untreated animals, the inhibitory transsynaptic effects p...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Olpe HR,Glatt A,Laszlo J,Schellenberg A

    更新日期:1980-03-17 00:00:00

  • Endogenous endothelin-1 initiates astrocytic growth after spinal cord injury.

    abstract::We developed a rat spinal cord injury model and investigated whether endogenous endothelin (ET)-1 plays a role in astrocytic growth after injury. Immunohistochemical study showed that the number of immature astrocytes (ACs) exhibiting strong reactivity to the monoclonal antibody, RC1, markedly increased 24 h after the...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Uesugi M,Kasuya Y,Hama H,Yamamoto M,Hayashi K,Masaki T,Goto K

    更新日期:1996-07-29 00:00:00

  • Interleukin-1 (IL-1) stimulates arginine vasopressin (AVP) release from superfused rat hypothalamo-neurohypophyseal complexes independently of cholinergic mechanism.

    abstract::We studied whether interleukin-1 (IL-1) affects the release of arginine vasopressin (AVP) from the superfused hypothalamo-neurohypophyseal complex (HNC) of rats. Involvement of the cholinergic system in the mediation of IL-1 on AVP release from HNC was also examined. Both human recombinant IL-1 alpha and -1 beta elici...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Nakatsuru K,Ohgo S,Oki Y,Matsukura S

    更新日期:1991-07-19 00:00:00

  • A neural system for evaluating the behavioural relevance of salient events outside the current focus of attention.

    abstract::Adaptive behaviour requires that organisms continuously monitor the environment for potentially significant information and evaluate whether currently unattended stimuli afford a behavioural change. Here we used event-related functional magnetic resonance imaging in humans to assess the neural mechanisms underlying th...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Gruber O,Diekhof EK,Kirchenbauer L,Goschke T

    更新日期:2010-09-10 00:00:00

  • Far field effects of seizure like events induced by application of 4-AP in combined entorhinal cortex hippocampal slices.

    abstract::Epileptiform activity induced by 4-AP in hippocampal area CA1 is characterised by short recurrent discharges. These are occasionally superimposed by slow field potential (fp) shifts. Simultaneous recordings of fps and [K(+)](o) in area CA1 and temporal cortex showed a slow fp shift in both regions, but associated rise...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Breustedt J,Gloveli T,Heinemann U

    更新日期:2002-11-22 00:00:00

  • Diphenylhydantoin protects against hypoxia-induced impairment of hippocampal synaptic transmission.

    abstract::The ability of diphenylhydantoin (DPH) to protect against hypoxia-induced neuronal damage was examined using electrophysiological recordings of extracellular evoked potentials from CA1 pyramidal neurons of rat hippocampal slices in vitro. In normal medium, a 15-min hypoxic insult (95% N2/5% CO2) produced rapid and com...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Stanton PK,Moskal JR

    更新日期:1991-04-19 00:00:00

  • High density of cholinergic muscarinic receptors accompanies high intensity acetylcholinesterase-staining in layer IV of infant rat somatosensory cortex.

    abstract::In this study the laminar localization of binding sites for the muscarinic radioligand [3H]3-quinuclidinylbenzilate was studied in somatosensory cortex of infant rat. Using light autoradiographic techniques, [3H]QNB was found to be concentrated in layer IV. Atropine-pretreated pups showed evenly dispersed, nonspecific...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Kristt DA,Kasper EK

    更新日期:1983-06-01 00:00:00

  • Alterations in phosphoinositide signaling and G-protein levels in depressed suicide brain.

    abstract::The function of the phosphoinositide signal transduction system and the levels of heterotrimeric G-protein alpha-subunits were examined in postmortem prefrontal cortex regions (8/9) and region (10) from suicide victims with major depression and matched control subjects without psychiatric illness. The hydrolysis of [3...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Pacheco MA,Stockmeier C,Meltzer HY,Overholser JC,Dilley GE,Jope RS

    更新日期:1996-06-03 00:00:00

  • Suppression of noradrenergic innervation compensates for behavioral deficits induced by lesion of dopaminergic terminals in the lateral septum.

    abstract::Spontaneous alternation which is disrupted by lesion of septal dopaminergic (DA) afferents was chosen as a behavioral marker for the study of functional interactions between DA and noradrenergic (NA) innervation of the lateral septum. Three groups of rats were studied: a solvent group which received only vehicle injec...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Taghzouti K,Le Moal M,Simon H

    更新日期:1991-06-21 00:00:00

  • Paced mating behavior in the female rat following lesions of three regions responsive to vaginocervical stimulation.

    abstract::The present study examines the effects of ibotenic acid lesions of the medial amygdala, the bed nucleus of the stria terminalis and the medial preoptic area on the display of paced mating behavior in female rats. Lesions of either the medial amygdala or the bed nucleus of the stria terminalis have no effect on the dis...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Guarraci FA,Megroz AB,Clark AS

    更新日期:2004-02-27 00:00:00

  • Nociceptin receptors in the rat spinal cord during morphine tolerance.

    abstract::The anatomical localization of nociceptin receptors was examined by in vitro quantitative autoradiography techniques in rat spinal cord sections by using [(125)I-Tyr(14)]nociceptin. [(125)I-Tyr(14)]nociceptin appeared to interact with a single class of binding sites (K(D)=0.1 nM) present in the grey matter in all lami...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Gouardères C,Tafani JA,Meunier JC,Jhamandas K,Zajac JM

    更新日期:1999-08-14 00:00:00

  • Nitric oxide increases stimulation-evoked acetylcholine release from rat hippocampal slices by a cyclic GMP-independent mechanism.

    abstract::Nitric oxide (NO) is an endothelium-derived relaxing factor and its main mechanism of action is activation of soluble guanylyl cyclase. NO and NO-related compounds have been reported to affect several neuronal functions in the central nervous system. In this study, we investigated the effects of NO donors (sodium nitr...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Suzuki T,Nakajima K,Fujimoto K,Fujii T,Kawashima K

    更新日期:1997-06-20 00:00:00

  • The response of striatal neuropeptide Y and cholinergic neurons to excitatory amino acid agonists.

    abstract::The effect of excitatory amino acid antagonists on antagonist on neuropeptide Y (NPY) and cholinergic neurons in the striatum of the rat was studied by means of NPY immunocytochemistry, DFP histochemistry for acetylcholinesterase (AChE), and biochemical determinations of choline acetyltransferase (ChAT). Intrastriatal...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Boegman RJ,Parent A

    更新日期:1988-06-14 00:00:00

  • Interactive effects of stimulation of D1 and D2 dopamine receptors on Fos expression in the lateral habenula.

    abstract::We have previously shown that systemic administration of non-selective dopamine agonists results in a pronounced expression of the proto-oncoprotein Fos within the lateral habenula. In the current study we examined the effects of selective D1 and D2 dopamine receptor agonists on habenular Fos expression. Rats were inj...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Wirtshafter D,Krebs JC

    更新日期:1997-03-07 00:00:00

  • The effects of GABA and benzodiazepines on neurones in the suprachiasmatic nucleus (SCN) of Syrian hamsters.

    abstract::Administration of benzodiazepines at appropriate times in the circadian cycle induce phase-shifts in circadian locomotor activity. The possibility that benzodiazepine-induced shifts are mediated at the level of the suprachiasmatic nuclei (SCN), identified as the circadian pacemaker in mammals, was examined electrophys...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Mason R,Biello SM,Harrington ME

    更新日期:1991-06-21 00:00:00

  • The excitatory effects of the specific benzodiazepine antagonist Ro14-7437, measured intracellularly in hippocampal CA1 cells.

    abstract::The specific benzodiazepine antagonist, Ro14-7437, in nanomolar concentrations, caused depolarization, increased spontaneous spiking, and conductance decrease when applied to CA1 cells in vitro. These effects were resistant to intracellularly injected Cl- ions or synaptic blockade by TTX, were prevented in Ca2+-free m...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Carlen PL,Gurevich N,Polc P

    更新日期:1983-07-18 00:00:00

  • Exposure to low concentrations of nicotine during cranial nerve development inhibits apoptosis and causes cellular hypertrophy in the ventral oculomotor nuclei of the chick embryo.

    abstract::Maternal cigarette use during pregnancy is associated with increased incidence of neural impairments in offspring, but nicotine's unique contribution to any neuropathology remains unclear, and nicotine's neurodevelopmental effects assessed in animal models vary with concentration. During ontogenesis, the chick oculomo...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Wielgus JJ,Corbin Downey L,Ewald KW,Hatley ME,Wilson KC,Yeilding RH

    更新日期:2004-03-12 00:00:00

  • Recovery of regenerating goldfish retinal ganglion cells is slowed in the absence of the topographically correct synaptic target.

    abstract::When the axons of goldfish retinal ganglion cells are severed the cell bodies undergo a series of changes as the axons regenerate. These changes begin to reverse when the axons start to innervate the tectum and by 3 months after the lesion the cell bodies have nearly returned to normal. When the axons projecting to th...

    journal_title:Brain research

    pub_type: 杂志文章


    authors: Burmeister DW,Dunn-Meynell AA

    更新日期:1987-10-13 00:00:00