Abstract:
:[d-Ala(2)-d-Leu(5)]-Enkephalin (DADLE) has shown promising results in protecting neurons from damages. However, the mechanism for this protection is still under investigation. The current study was carried out to test the hypothesis that DADLE may regulate cellular transcription in neurons. SH-SY5Y cells and primary cortical neurons were treated with various doses of DADLE for 24-72h. Results demonstrated that DADLE, at all doses and time points examined, significantly inhibited cellular transcription in both cells without causing cell injury. Following recovery for 72h without DADLE in primary neurons, the transcriptional activity fully resumed. Delta opioid receptor (DOR) is not involved in this process, as Naltrindole could not abolish DADLE׳s transcriptional inhibitory effects. Further studies in primary cortical neurons show that DADLE significantly inhibited phosphorylation of Ser2 and Ser5 of the C-terminal domain (CTD) of RNA polymerase II. These data indicate that DADLE is able to decrease cellular transcription through inhibiting phosphorylation of RNA polymerase II in neurons, which may provide mechanistic insight into its reported neuroprotective effects, and suggests that it warrants further exploration as a potential therapeutic strategy for neuroprotection.
journal_name
Brain Resjournal_title
Brain researchauthors
Tian J,Gu Y,Sun K,Wang B,Chen J,Wang X,Su Ddoi
10.1016/j.brainres.2014.04.007subject
Has Abstractpub_date
2014-05-27 00:00:00pages
1-7eissn
0006-8993issn
1872-6240pii
S0006-8993(14)00484-3journal_volume
1565pub_type
杂志文章相关文献
BRAIN RESEARCH文献大全abstract::The respiratory function of cerebral mitochondria harvested from genetically diabetic (BB/W) and streptozotocin-diabetic rats deprived of insulin for 3-4 weeks was found to be unchanged from control values. Furthermore, insulin-deprived BB/W rats subjected to 30 min of insulin-induced hypoglycemic coma demonstrated a ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(89)91624-7
更新日期:1989-02-13 00:00:00
abstract::Mammalian neostriatum is composed of two neurochemically and neuroanatomically defined compartments, called the patches and matrix. The present study concerns a search for neurochemical molecules involved in formation of the striatal compartments. Using the monoclonal antibody CR-50, we here disclose a transient expre...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(99)02136-8
更新日期:1999-12-11 00:00:00
abstract::Neuronal cells in primary culture have been demonstrated to possess specific insulin receptors (Boyd et al., J. Biol. Chem., 260 (1985) 15880-15884). Incubation of these cultures with insulin causes a dose-dependent inhibition of maprotiline-sensitive [3H]norepinephrine uptake. Maximum inhibition of 95% of maprotiline...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(86)91242-4
更新日期:1986-11-19 00:00:00
abstract::Chemotherapy-induced pain is the most common treatment-limiting complication encountered by cancer patients receiving taxane-, vinca alkaloid- or platin-based chemotherapy. Several lines of evidence indicate that activation of pro-inflammatory cascades involving the release of cytokines including tumor necrosis factor...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2008.07.001
更新日期:2008-09-10 00:00:00
abstract::Transforming growth factor beta (TGFbeta) modulates a variety of growth related functions following traumatic injury. The cellular response to TGFbeta is predominantly mediated through TGFbeta receptor I (TGFbetaRI) and receptor II (TGFbetaRII) on the cell surface and SMAD proteins intracellularly. We investigated the...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2004.03.028
更新日期:2004-06-25 00:00:00
abstract::Traumatic brain injury (TBI) affects 2.8 million people annually in the United States, with significant populations suffering from ongoing cognitive dysfunction. Impairments in decision-making can have major implications for patients and their caregivers, often enduring for years to decades, yet are rarely explored in...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2018.10.004
更新日期:2019-02-01 00:00:00
abstract::The effects of Morris water maze training on brain metabolism and behavior were compared between aged (20-22 months) and young (2-4 months) Fischer 344 male rats. Each group had yoked controls, which swam the same amount of time as the trained rats but without the platform. This was followed after 9 days by quantitati...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(02)02545-3
更新日期:2002-06-07 00:00:00
abstract::Antibody microprobes were used to study the release of immunoreactive neurokinin A into the spinal cord of anaesthetised cats during and following injection of a knee joint with kaolin and carrageenan. A basal level of immunoreactive neurokinin A was detected prior to any noxious stimuli. Innocuous mechanical joint st...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(90)91352-h
更新日期:1990-11-19 00:00:00
abstract::The first phase of this study involves injecting a different fluorescent retrograde axonal tracer into the subthalamic nucleus, the substantia nigra, and the mesopontine tegmentum. Multiple labeled cells are found within the caudal third of the globus pallidus. The entopeduncular nucleus and adjacent basal forebrain s...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(89)90948-7
更新日期:1989-05-15 00:00:00
abstract::Previous research has demonstrated that cross-language activation is present even when proficient bilinguals perform a task only in one language. The present study investigated the time-course of cross-language activation during word production in a second language (L2) by using a picture-word interference paradigm wi...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2010.11.053
更新日期:2011-01-31 00:00:00
abstract::The effect of cerebellar stimulation on the accommodation of the lens was examined in anesthetized cats. An infrared optometer was used to measure the refractive power of the lens during stimulation of the cerebellum. The area giving responses within latencies shorter than 160 msec and amplitudes larger than 0.15 diop...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(78)90334-7
更新日期:1978-09-29 00:00:00
abstract::In epilept WAG/Rij rats, multiple unit activity coinciding with the occurrence of spike-wave discharges was recorded under neurolept anesthesia. Recordings were made in the frontal cortex and in various nuclei of the thalamus, in specific nuclei such as the ventroposterolateral, the ventroposteromedial and the ventrol...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(93)91641-5
更新日期:1993-05-28 00:00:00
abstract::Mounting evidence suggests that sleep loss negatively affects learning and memory processes through disruption of hippocampal function. In the present study, we examined whether sleep loss alters the generation, differentiation, and survival of new cells in the dentate gyrus. Rats were sleep restricted by keeping them...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2005.10.020
更新日期:2005-12-14 00:00:00
abstract::Prostanoids in the central nervous system define an important linkage between blood pressure and hormonal responses to hypotension/ischemia. Prostaglandin endoperoxide synthase (PGHS)-2, the inducible isoform of this enzyme, is induced by cerebral hypoperfusion/ischemia. To investigate the mechanism of the PGHS-2 gene...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2005.08.033
更新日期:2005-10-26 00:00:00
abstract::DL-N-(2-Hydroxy-3-napthyloxypropyl)-N'-dansylethylenediamine, dansyl analogue of propranolol (DAPN) is a novel fluorescent beta-adrenergic antagonist with high affinity to beta-receptors. The distribution pattern of DAPN fluorescence was studied in the rat central nervous system subsequent to its intravenous administr...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(78)90288-3
更新日期:1978-07-14 00:00:00
abstract::Mapping studies with central drug injections in the hungry rat have identified the perifornical lateral hypothalamus as being uniquely sensitive to the feeding suppressive effects of exogenous dopamine, as well as anorexic drugs which release endogenous catecholamines. In the present study, the hypothalamic dopamine-r...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(79)90899-0
更新日期:1979-08-17 00:00:00
abstract::The effects of retinoic acid (RA), a naturally occurring metabolite of vitamin A, on the growth, morphology and neurochemical differentiation of the PC12 clone of rat pheochromocytoma cells were investigated. RA added to the medium inhibited the growth of PC12 cells in a dose-dependent manner up to 10 microM without a...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(89)90460-5
更新日期:1989-11-13 00:00:00
abstract::A cyto- and myeloarchitectonic parcellation of the superior temporal sulcus and surrounding cortex in the rhesus monkey has been correlated with the pattern of afferent cortical connections from ipsilateral temporal, parietal and occipital lobes, studied by both silver impregnation and autoradiographic techniques. The...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(78)90584-x
更新日期:1978-06-23 00:00:00
abstract::Centrifugal fibers and their synaptic connections were studied in retinas of the lamprey Lampetra fluviatilis. The morphological analysis of retinofugal and retinopetal elements was performed after their horseradish peroxidase (HRP) filling through either the cut optic nerve in isolated retina preparations or after in...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(89)90999-2
更新日期:1989-07-24 00:00:00
abstract::Neurons from many brainstem nuclei involved in respiratory control increase their firing rate in response to acidosis in vitro, suggesting that they are central chemoreceptors. This property has been considered to be either unique to neurons involved in respiratory control, or at least very unusual for non-respiratory...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(00)02033-3
更新日期:2000-03-31 00:00:00
abstract::The effects of stimulating the reticular formation were studied during fictive locomotion in lampreys (Ichthyomyzon unicuspis). The in vitro isolated preparation of the brainstem and spinal cord was used and fictive locomotion was induced by bath application of N-methyl-D-aspartate (NMDA; 50-100 microM). During differ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(97)00151-0
更新日期:1997-04-11 00:00:00
abstract::In rats anaesthetised with alphaxalone/alphadolone, electrical stimulation in the dorsolateral part of the periaqueductal grey matter (PAG; 10 s trains of 1 ms pulses at 80 Hz, 40-80 microA) evoked a pressor response accompanied by tachycardia. Both components of the response were attenuated following microinjection o...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(94)90702-1
更新日期:1994-07-18 00:00:00
abstract::Confocal microscopy and the Ca(2+)-sensitive fluorescent dye fluo-3 were used to study subcellular Ca2+ signals in embryonic, neonatal, and adult dorsal root ganglion (DRG) neurons in excised dorsal root ganglia. Optical images obtained from isolated whole embryonic and neonatal ganglia revealed a marked variability i...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(94)91444-3
更新日期:1994-01-28 00:00:00
abstract::Rab3A is a small GTP-binding synaptic vesicle protein, shown to dissociate from synaptic vesicle membranes upon depolarization-induced exocytosis. Using an antiserum raised against rab3A, we found that the antigen was localized to the neuropil of specific brain regions, but was not present in major fiber tracts or mos...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(92)91087-u
更新日期:1992-09-11 00:00:00
abstract::Examination of glycolmethacrylate embedded olfactory bulbs of normal rats revealed that the granule cells of the accessory olfactory bulb were dissimilar from the majority of main olfactory bulb granule cells. Cells from these structures can be discriminated on the basis of spherical form. nuclear diameter, nucleoplas...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(82)90711-9
更新日期:1982-03-25 00:00:00
abstract::Nicergoline, a drug used for the treatment of Alzheimer's disease and other types of dementia, was tested for its ability to protect neurons against beta-amyloid toxicity. Pure cultures of rat cortical neurons were challenged with a toxic fragment of beta-amyloid peptide (betaAP(25-35)) and toxicity was assessed after...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2005.04.004
更新日期:2005-06-14 00:00:00
abstract::The projection from the vestibular and perihypoglossal nuclei to the spinal trigeminal and lateral reticular nuclei has been studied in cats where the wheat germ agglutinin-horseradish peroxidase complex has been used as a retrograde tracer. All injections were made at the level of the caudal pole of the inferior oliv...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(85)90131-3
更新日期:1985-04-29 00:00:00
abstract::The role of the lateral parabrachial area (lPB) in the acquisition of a delayed taste aversion learning task (TAL) was examined by delivering the peripherally acting aversive compound, methylscopolamine (MSP), through two different routes, intraperitoneal and intraventricular. Consistent with previous anatomical, beha...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(00)02453-7
更新日期:2000-07-14 00:00:00
abstract::Large bilateral aspiration lesions of the hippocampus had no significant effect on septal kindling, whereas large bilateral DC lesions of the pyriform lobe resulted in a small but significant increase in the number of septal stimulations required to complete kindling. Bilateral aspiration lesions of the dorsal hippoca...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(88)90826-8
更新日期:1988-06-28 00:00:00
abstract::Systemic administration of mesenchymal stem cells (MSCs) following cerebral infarction exerts functional improvements. Previous research has suggested potential therapeutic mechanisms that promote neuroprotection and synaptogenesis. These include secretion of neurotrophic factors, remodeling of neural circuits, restor...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2018.05.033
更新日期:2018-09-15 00:00:00