Abstract:
:Conventional nanoporous hydrogels often lead to slow cartilage deposition by MSCs in 3D due to physical constraints and requirement for degradation. Our group has recently reported macroporous gelatin microribbon (μRB) hydrogels, which substantially accelerate MSC-based cartilage formation in vitro compared to conventional gelatin hydrogels. To facilitate translating the use of μRB-based scaffolds for supporting stem cell-based cartilage regeneration in vivo, there remains a need to develop a customize-designed drug delivery system that can be incorporated into μRB-based scaffolds. Towards this goal, here we report polydopamine-coated mesoporous silica nanoparticles (MSNs) that can be stably incorporated within the macroporous μRB scaffolds, and allow tunable release of transforming growth factor (TGF)-β3. We hypothesize that increasing concentration of polydopamine coating on MSNs will slow down TGF- β3 release, and TGF-β3 release from polydopamine-coated MSNs can enhance MSC-based cartilage formation in vitro and in vivo. We demonstrate that TGF-β3 released from MSNs enhance MSC-based cartilage regeneration in vitro to levels comparable to freshly added TGF-β3 in the medium, as shown by biochemical assays, mechanical testing, and histology. Furthermore, when implanted in vivo in a mouse subcutaneous model, only the group containing MSN-mediated TGF-β3 release supported continuous cartilage formation, whereas control group without MSN showed loss of cartilage matrix and undesirable endochondral ossification. The modular design of MSN-mediated drug delivery can be customized for delivering multiple drugs with individually optimized release kinetics, and may be applicable to enhance regeneration of other tissue types.
journal_name
Ann Biomed Engjournal_title
Annals of biomedical engineeringauthors
Barati D,Gegg C,Yang Fdoi
10.1007/s10439-020-02522-zsubject
Has Abstractpub_date
2020-07-01 00:00:00pages
1971-1981issue
7eissn
0090-6964issn
1573-9686pii
10.1007/s10439-020-02522-zjournal_volume
48pub_type
杂志文章abstract::Our 3-D model for a junctional complex (JC) in the erythrocyte membrane skeleton proposed that the helical actin protofilament functions as a mechanical axis for three pairs of αβ spectrin (Sp), and each pair wraps around the protofilament in a back-to-back fashion. The distal end of each Sp is further associated with...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1007/s10439-011-0293-6
更新日期:2011-07-01 00:00:00
abstract::Abnormal scapular movement is widely believed to be an important factor in clinical pathology of the shoulder joint complex. Validated non-invasive techniques for measuring scapular movement have been developed, but the effect of marker placement on accuracy is unknown. The objective of this study was to determine the...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1007/s10439-010-0185-1
更新日期:2011-02-01 00:00:00
abstract::We present a method for the localized statistical discrimination of class populations based on the Karhunen-Loève and Fukunaga-Koontz transforms. These transforms provide features that model the variance of a sample distribution. The spatial series of a 196 channel epicardial electrogram recording from an arrythmogeni...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1007/BF02367084
更新日期:1994-09-01 00:00:00
abstract::A recent analytical solution of the three-dimensional Stokes flow through a bumpy tube predicts that for a given bump area, there exists an optimal circumferential wavenumber which minimizes flow resistance. This study uses measurements of microvessel endothelial cell morphology to test whether this prediction holds i...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1007/s10439-008-9467-2
更新日期:2008-04-01 00:00:00
abstract::Linear compartmental models are useful, explanatory tools, that have been widely used to represent the dynamic behavior of complex biological systems. This paper addresses the problem of the numerical identification of such models, i.e., the estimation of the parameter values that will generate predictions closest to ...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1007/s10439-009-9651-z
更新日期:2009-05-01 00:00:00
abstract::Premature ovarian insufficiency (POI) is a major complication of cytotoxic treatments due to extreme ovarian sensitivity to chemotherapy and radiation. In pediatric cancer patients modern therapy has improved the long-term survival to over 80% in the United States. However, these cancer survivors face long-term health...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1007/s10439-016-1780-6
更新日期:2017-07-01 00:00:00
abstract::In order to successfully use a model for parameter identification, it must be carefully analyzed. Current analysis methods, however, are ad hoc and provide only partial information. We extended these methods through the application of stacked dimensions, a scientific visualization method. The end result of our extensi...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1007/BF02771001
更新日期:1996-01-01 00:00:00
abstract::In order to increase the probability of having a successful cataract post-surgery, the customisation of the haptic design of the intraocular lens (IOL) according to the characteristics of the patient is recommended. In this study, we present two prediction models based on deep neural networks (DNNs). One is capable of...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1007/s10439-020-02636-4
更新日期:2020-12-01 00:00:00
abstract::Blunt carotid artery injuries occur in 0.3% of blunt injured patients and may lead to devastating neurological consequences. However, arterial mechanics leading to internal layer subfailure have not been quantified. Twenty-two human carotid artery segments and 18 porcine thoracic aorta segments were opened to expose t...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1007/s10439-006-9229-y
更新日期:2007-02-01 00:00:00
abstract::A novel apparatus for the multifaceted evaluation of artery function was developed. It measures endothelial and smooth muscle functions and the pressure-strain elastic modulus (E p). A rigid airtight chamber with an ultrasound probe was attached to the upper arm to manipulate the transmural pressure of the brachial ar...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1007/s10439-017-1810-z
更新日期:2017-06-01 00:00:00
abstract::This is a personal historical essay on meanderings through the jungle of the microcirculatory swamp. Because one pretends that the wandering was purposefully exploratory, a few guideposts are placed at positions where one could discern blaze-marks from earlier wanderers, and the path cut a little wider along some of t...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章,评审
doi:10.1007/BF02363568
更新日期:1987-01-01 00:00:00
abstract::The effects of facet wiring procedure commonly used for stabilizing cervical spines after laminectomy or bilateral facet dislocation on the motion behavior of whole cervical spines are investigated using a Selspot II system. A fresh human ligamentous intact specimen was potted at T1/T2 vertebra and clinically relevant...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1007/BF02368022
更新日期:1989-01-01 00:00:00
abstract::A major drawback in the operation of mechanical heart valve prostheses is thrombus formation in the near valve region. Detailed flow analysis in this region during the valve closure phase is of interest in understanding the relationship between shear stress and platelet activation. A fixed-grid Cartesian mesh flow sol...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1007/s10439-006-9194-5
更新日期:2006-10-01 00:00:00
abstract::Diabetic retinopathy is the leading cause of blindness in working age individuals in the United States. Breakdown of the blood-retinal barrier is one of the earliest events in the progression of diabetic retinopathy. Ideally, therapeutic measures would be directed at this early stage, but there are few sensitive, quan...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1114/1.1385809
更新日期:2001-08-01 00:00:00
abstract::Studying human cancer from a biomechanical perspective may contribute to pathogenesis understanding which leads to the malignancy. In this study, biomechanics of suspended and adhered breast cancer cells were investigated via the micropipette aspiration method with special emphasis on comparing the cell stiffness and ...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1007/s10439-019-02284-3
更新日期:2019-08-01 00:00:00
abstract::This project was undertaken to establish if the integral circulation had any effects upon the mechanical behavior of bone. The influence of blood flow in the rabbit tibia on the strain induced at the bone surface when under load was assessed. Recordings were taken from three adult and three immature New Zealand White ...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1007/BF02390377
更新日期:1994-03-01 00:00:00
abstract::Microfluidic devices fabricated using poly(dimethylsiloxane) (PDMS) polymer are routinely used for in vitro cell culture for a wide range of cellular assays. These assays typically involve the incubation of cultured cells with a drug molecule or a fluorescent marker while monitoring a cellular response. The accuracy o...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1007/s10439-012-0562-z
更新日期:2012-09-01 00:00:00
abstract::The material properties of normal cadaveric human cartilage in the ankle mortice (tibiotalar articulation) were evaluated to determine a possible etiologic mechanism of cartilage injury of the ankle when an obvious traumatic episode is not present. Using an automated indentation apparatus and the biphasic creep indent...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1007/BF02584467
更新日期:1995-09-01 00:00:00
abstract::Neuromuscular impairment associated with cerebral palsy (CP) often leads to life-long walking deficits. Our goal was to evaluate the ability of a novel untethered wearable ankle exoskeleton to reduce the severity of gait pathology from CP. In this clinical feasibility study of five individuals with CP, we used instrum...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1007/s10439-019-02237-w
更新日期:2019-06-01 00:00:00
abstract::Platelet thrombosis under arterial conditions remains a large clinical problem. Previous in vitro experiments have concentrated on early adherence without thrombotic occlusion. We have developed a controllable hemodynamic system that creates intravascular thrombosis to occlusion. Lightly heparinized (3.5 USP units/mL)...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1007/s10439-011-0296-3
更新日期:2011-07-01 00:00:00
abstract::Zebrafish is an emerging model system for cardiac conduction and regeneration. Zebrafish heart regenerates after 20% ventricular resection within 60 days. Whether cardiac conduction phenotype correlated with cardiomyocyte regeneration remained undefined. Longitudinal monitoring of the adult zebrafish heart (n = 12) wa...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1007/s10439-010-9993-6
更新日期:2010-07-01 00:00:00
abstract::Tissue engineering strategies have utilized a wide spectrum of synthetic and naturally-derived scaffold materials. Synthetic scaffolds are better defined and offer the ability to precisely and reproducibly control their properties, while naturally-derived scaffolds typically have inherent biological and structural pro...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章,评审
doi:10.1007/s10439-014-1192-4
更新日期:2015-03-01 00:00:00
abstract::Long-range dependency has been found in most rhythmic motor signals. The origin of this property is unknown and largely debated. There is a controversy on the influence of voluntary control induced by requiring a pre-determined pace such as asking subjects to step to a metronome. We studied the cycle duration variabil...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章,随机对照试验
doi:10.1007/s10439-013-0834-2
更新日期:2013-08-01 00:00:00
abstract::To meet demands of vascular reconstruction, there is a need for prosthetic alternatives to natural blood vessels. Here we explored a new conduit fabrication approach. Macroporous, gelatin microcarriers laden with human umbilical vein endothelial cells and aortic smooth muscle cells were dispensed into tubular agarose ...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1007/s10439-013-0883-6
更新日期:2014-07-01 00:00:00
abstract::The induction of angiogenesis is a promising therapeutic strategy for the amelioration of peripheral arterial disease (PAD). This occlusive disease results in muscle ischemia, and neovascularization is a route to increasing the perfusion in the tissue. The vascular endothelial growth factor (VEGF) family of potent pro...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1007/s10439-007-9303-0
更新日期:2007-06-01 00:00:00
abstract::The statistical distribution of von Mises stress in the trabeculae of human vertebral cancellous bone was estimated using large-scale finite element models. The goal was to test the hypothesis that average trabecular von Mises stress is correlated to the maximum trabecular level von Mises stress. The hypothesis was pr...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1114/1.1318928
更新日期:2000-01-01 00:00:00
abstract::Despite the use of helmets in American football, brain injuries are still prevalent. To reduce the burden of these injuries, novel impact mitigation systems are needed. The Vicis Zero1 (VZ1) American football helmet is unique in its use of multi-directional buckling structures sandwiched between a deformable outer she...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1007/s10439-020-02472-6
更新日期:2020-05-01 00:00:00
abstract::In previous shoulder impact studies, the 50th-percentile male GHBMC human body finite-element model was shown to have good biofidelity regarding impact force, but under-predicted shoulder deflection by 80% compared to those observed in the experiment. The goal of this study was to validate the response of the GHBMC M5...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1007/s10439-015-1546-6
更新日期:2016-08-01 00:00:00
abstract::Polycrystalline silicon (poly-Si) micromechanical structures can be made by selectively etching an underlying sacrificial oxide layer. Advantages of this micromachining technique are its simplicity and compatibility with conventional integrated-circuit processing. Compressive internal stress in poly-Si films constrain...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1007/BF02584269
更新日期:1986-01-01 00:00:00
abstract::Phospholipids have been identified by thin-layer chromatography in appreciable quantities in pericardial fluid taken from 12 dogs and found to include sphingomyelin and phosphatidylcholines, -ethanolamines, -inositols and -serines--the cholines predominating. The extracts, the synthetic surfactants and a mixture of sy...
journal_title:Annals of biomedical engineering
pub_type: 杂志文章
doi:10.1007/BF02584258
更新日期:1985-01-01 00:00:00