Abstract：
:Studying human cancer from a biomechanical perspective may contribute to pathogenesis understanding which leads to the malignancy. In this study, biomechanics of suspended and adhered breast cancer cells were investigated via the micropipette aspiration method with special emphasis on comparing the cell stiffness and viscoelastic parameters of estrogen receptor positive, ER+, MCF-7 and human epidermal growth factor receptor 2 positive, HER2 +, SKBR-3 cancer cell lines prior to and post treatment with tamoxifen and trastuzumab, respectively. Alterations of mechanical parameters included significant increase in cell stiffness, especially after treatment with trastuzumab and changes in viscoelastic parameters, in both cancer cell lines post treatment. According to immunofluorescence analysis, the raised cell stiffness was corresponded to remodeling of F-actin, which peripherally located in tamoxifen treated and perinuclear accumulated in trastuzumab treated cancer cell cytoskeleton, implying a reduced potential for cell deformation and motility. Additionally, these results were in line with the study of single and collective cell migration through Boyden chamber and wound healing assays respectively, where the potential for migration was significantly decreased after treatment. Consequently, these findings lead to an increased interest in biomechanics of cancer progression after treatment with anti-tumor agents, importantly in understanding the effect of the alterations of mechanical properties upon the possibility for change in metastatic potential.
journal_name
Ann Biomed Engjournal_title
Annals of biomedical engineeringauthors
Metsiou DN,Siatis KE,Giannopoulou E,Papachristou DJ,Kalofonos HP,Koutras A,Athanassiou Gdoi
10.1007/s10439-019-02284-3subject
Has Abstractpub_date
2019-08-01 00:00:00pages
1711-1724issue
8eissn
0090-6964issn
1573-9686pii
10.1007/s10439-019-02284-3journal_volume
47pub_type
杂志文章abstract：:There are recent reports of hybrid tissue-fabric materials with good performance-high biocompatibility and high mechanical strength. In this study, we demonstrate the capability of a hybrid material as a long-term filter for blood proteins. Polyester fabrics were implanted into rats to fabricate hybrid tissue-fabric m...
journal_title：Annals of biomedical engineering
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journal_title：Annals of biomedical engineering
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doi：10.1007/s10439-011-0372-8
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abstract：:A major benefit of computational modeling in biomechanics research is its ability to estimate internal muscular demands given limited input information. However, several assumptions regarding model parameters and constraints may influence model outputs. This research evaluated the influence of model parameter variabil...
journal_title：Annals of biomedical engineering
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doi：10.1007/s10439-014-1035-3
更新日期：2014-09-01 00:00:00
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journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-009-9773-3
更新日期：2009-11-01 00:00:00
abstract：:The responses of photoreceptor cells to moving stimuli are crucial to understanding motion detection in visual systems. However, these responses are not well characterized quantitatively because they result from a combination of spatial optical behavior in the lens systems with temporal behavior in the phototransducti...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1114/1.58
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abstract：:We attempted to estimate in living adherent epithelial alveolar cells, the degree of structural and mechanical heterogeneity by considering two individualized cytoskeleton components, i.e., a submembranous "cortical" cytoskeleton and a "deep" cytoskeleton (CSK). F-actin structure characterizing each CSK component was ...
journal_title：Annals of biomedical engineering
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abstract：:In order to increase the probability of having a successful cataract post-surgery, the customisation of the haptic design of the intraocular lens (IOL) according to the characteristics of the patient is recommended. In this study, we present two prediction models based on deep neural networks (DNNs). One is capable of...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
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abstract：:Viscous shear stress and Reynolds stress are often used to predict hemolysis and thrombosis due to flow-induced stress on blood elements in cardiovascular devices. These macroscopic stresses are distinct from the true stress on an individual cell, which is determined by the local microscale flow field. In this paper t...
journal_title：Annals of biomedical engineering
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doi：10.1007/s10439-007-9308-8
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journal_title：Annals of biomedical engineering
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abstract：:Magnetic nanoparticle (mNP) based thermal therapies have demonstrated relevance in the clinic, but effective application requires an understanding of both its strengths and limitations. This study explores two critical limitations for clinical use: (1) maximizing localized mNP heating, while avoiding bulk heating due ...
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journal_title：Annals of biomedical engineering
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doi：10.1007/s10439-019-02245-w
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journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-009-9851-6
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journal_title：Annals of biomedical engineering
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journal_title：Annals of biomedical engineering
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journal_title：Annals of biomedical engineering
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journal_title：Annals of biomedical engineering
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doi：10.1007/s10439-014-1057-x
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journal_title：Annals of biomedical engineering
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journal_title：Annals of biomedical engineering
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journal_title：Annals of biomedical engineering
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journal_title：Annals of biomedical engineering
pub_type： 临床试验,杂志文章
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journal_title：Annals of biomedical engineering
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journal_title：Annals of biomedical engineering
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journal_title：Annals of biomedical engineering
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journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/BF02368609
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