Cellularized microcarriers as adhesive building blocks for fabrication of tubular tissue constructs.

Abstract:

:To meet demands of vascular reconstruction, there is a need for prosthetic alternatives to natural blood vessels. Here we explored a new conduit fabrication approach. Macroporous, gelatin microcarriers laden with human umbilical vein endothelial cells and aortic smooth muscle cells were dispensed into tubular agarose molds and found to adhere to form living tubular tissues. The ability of cellularized microcarriers to adhere to one another involved cellular and extracellular matrix bridging that included the formation of epithelium-like cell layers lining the lumenal and ablumenal surfaces of the constructs and the deposition of collagen and elastin fibers. The tubular tissues behaved as elastic solids, with a uniaxial mechanical response that is qualitatively similar to that of native vascular tissues and consistent with their elastin and collagen composition. Linearized measures of the mechanical response of the fabricated tubular tissues at both low and high strains were observed to increase with duration of static culture, with no significant loss of stiffness following decellularization. The findings highlight the utility of cellularized macroporous gelatin microcarriers as self-adhering building blocks for the fabrication of living tubular structures.

journal_name

Ann Biomed Eng

authors

Twal WO,Klatt SC,Harikrishnan K,Gerges E,Cooley MA,Trusk TC,Zhou B,Gabr MG,Shazly T,Lessner SM,Markwald RR,Argraves WS

doi

10.1007/s10439-013-0883-6

subject

Has Abstract

pub_date

2014-07-01 00:00:00

pages

1470-81

issue

7

eissn

0090-6964

issn

1573-9686

journal_volume

42

pub_type

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