Cellularized microcarriers as adhesive building blocks for fabrication of tubular tissue constructs.


:To meet demands of vascular reconstruction, there is a need for prosthetic alternatives to natural blood vessels. Here we explored a new conduit fabrication approach. Macroporous, gelatin microcarriers laden with human umbilical vein endothelial cells and aortic smooth muscle cells were dispensed into tubular agarose molds and found to adhere to form living tubular tissues. The ability of cellularized microcarriers to adhere to one another involved cellular and extracellular matrix bridging that included the formation of epithelium-like cell layers lining the lumenal and ablumenal surfaces of the constructs and the deposition of collagen and elastin fibers. The tubular tissues behaved as elastic solids, with a uniaxial mechanical response that is qualitatively similar to that of native vascular tissues and consistent with their elastin and collagen composition. Linearized measures of the mechanical response of the fabricated tubular tissues at both low and high strains were observed to increase with duration of static culture, with no significant loss of stiffness following decellularization. The findings highlight the utility of cellularized macroporous gelatin microcarriers as self-adhering building blocks for the fabrication of living tubular structures.


Ann Biomed Eng


Twal WO,Klatt SC,Harikrishnan K,Gerges E,Cooley MA,Trusk TC,Zhou B,Gabr MG,Shazly T,Lessner SM,Markwald RR,Argraves WS




Has Abstract


2014-07-01 00:00:00












  • Role of vortices in growth of microbubbles at mitral mechanical heart valve closure.

    abstract::This study is aimed at refining our understanding of the role of vortex formation at mitral mechanical heart valve (MHV) closure and its association with the high intensity transient signals (HITS) seen in echocardiographic studies with MHV recipients. Previously reported numerical results described a twofold process ...

    journal_title:Annals of biomedical engineering

    pub_type: 杂志文章


    authors: Rambod E,Beizai M,Sahn DJ,Gharib M

    更新日期:2007-07-01 00:00:00

  • Quadratic phase coupling as a quantitative measure for the developing hippocampal formation.

    abstract::This paper presents the bispectral analysis of the ontogeny of the hippocampal EEG recorded from the dentate gyrus and CA1, the primary sites that generate theta (theta) rhythm. The hippocampal EEG was collected during the vigilance state of rapid eye movement (REM) sleep of freely moving rats at 15, 30 and 90 days of...

    journal_title:Annals of biomedical engineering

    pub_type: 杂志文章


    authors: Ning T,Bronzino JD

    更新日期:1998-07-01 00:00:00

  • Development of a side-view chamber for studying cell-surface adhesion under flow conditions.

    abstract::Observing microscopic specimens is often useful in studies of cellular interaction with a vascular wall. We have developed an in vitro side-view flow chamber that permits observations from the side of the cell's contact with various adhesive surfaces under dynamic flow conditions. This side-view flow chamber consists ...

    journal_title:Annals of biomedical engineering

    pub_type: 杂志文章


    authors: Cao J,Usami S,Dong C

    更新日期:1997-05-01 00:00:00

  • A piconewton force transducer and its application to measurement of the bending stiffness of phospholipid membranes.

    abstract::The bending stiffness of a phospholipid bilayer (Kc) was measured by forming thin bilayer cylinders (tethers) from giant phospholipid vesicles. Based on the balance of forces, the tether force was expected to be proportional to the square root of the membrane tension, with a constant of proportionality containing Kc. ...

    journal_title:Annals of biomedical engineering

    pub_type: 杂志文章


    authors: Heinrich V,Waugh RE

    更新日期:1996-09-01 00:00:00

  • Machine Learning Classification of Verified Head Impact Exposure Strengthens Associations with Brain Changes.

    abstract::Cumulative exposure to head impacts during contact sports can elicit potentially deleterious brain white matter alterations in young athletes. Head impact exposure is commonly quantified using wearable sensors; however, these sensors tend to overestimate the number of true head impacts that occur and may obfuscate pot...

    journal_title:Annals of biomedical engineering

    pub_type: 杂志文章


    authors: DiCesare CA,Green B,Yuan W,Diekfuss JA,Barber Foss KD,Dudley J,Qin Y,Wang P,Myer GD

    更新日期:2020-12-01 00:00:00

  • Mechanical behavior of vascular smooth muscle in cylindrical segments of arteries in vitro.

    abstract::Vascular smooth muscle mechanics have been studied in vitro in cylindrical segments of dog carotid artery, human internal mammary artery, and human saphenous vein. Such cylindrical preparations maintain normal vessel geometry and also permit correlation of mechanical phenomena with transmural pressure. These studies s...

    journal_title:Annals of biomedical engineering

    pub_type: 杂志文章


    authors: Dobrin PB

    更新日期:1984-01-01 00:00:00

  • Development of an endothelial-smooth muscle cell coculture model using phenotype-controlled smooth muscle cells.

    abstract::A coculture of endothelial cells (ECs) and smooth muscle cells (SMCs), which mimics cellular interactions appearing in vivo, has been performed in studies on the relationship between atherogenesis and fluid shear stress conditions. Although healthy arteries in vivo consist of contractile phenotype SMCs, cultured cells...

    journal_title:Annals of biomedical engineering

    pub_type: 杂志文章


    authors: Sakamoto N,Kiuchi T,Sato M

    更新日期:2011-11-01 00:00:00

  • Investigation of the Sequential Actions of Doxorubicin and p53 on Tumor Cell Growth Via Branched Polyethylenimine-β-cyclodextrin Conjugates.

    abstract::The combination of gene therapy and chemotherapy has showed increased therapeutic efficacy in the treatment of cancers, but it is not well investigated about the actual coordination pattern between therapeutic gene and chemical drug. In this work, p53/BPEI-β-CD/AD-dox complex was fabricated and employed to investigate...

    journal_title:Annals of biomedical engineering

    pub_type: 杂志文章


    authors: Xie B,Peng J,Wang S,Zhang X,Nie H

    更新日期:2016-11-01 00:00:00

  • 3D Printing of Calcium Phosphate Ceramics for Bone Tissue Engineering and Drug Delivery.

    abstract::Additive manufacturing, also known as 3D printing, has emerged over the past 3 decades as a disruptive technology for rapid prototyping and manufacturing. Vat polymerization, powder bed fusion, material extrusion, and binder jetting are distinct technologies of additive manufacturing, which have been used in a wide va...

    journal_title:Annals of biomedical engineering

    pub_type: 杂志文章,评审


    authors: Trombetta R,Inzana JA,Schwarz EM,Kates SL,Awad HA

    更新日期:2017-01-01 00:00:00

  • High-resolution measurement of the unsteady velocity field to evaluate blood damage induced by a mechanical heart valve.

    abstract::We investigate the potential of prosthetic heart valves to generate abnormal flow and stress patterns, which can contribute to platelet activation and lysis according to blood damage accumulation mechanisms. High-resolution velocity measurements of the unsteady flow field, obtained with a standard particle image veloc...

    journal_title:Annals of biomedical engineering

    pub_type: 杂志文章


    authors: Bellofiore A,Quinlan NJ

    更新日期:2011-09-01 00:00:00

  • Through the microcirculatory maze with machete, molecule, and minicomputer (1986 Alza lecture).

    abstract::This is a personal historical essay on meanderings through the jungle of the microcirculatory swamp. Because one pretends that the wandering was purposefully exploratory, a few guideposts are placed at positions where one could discern blaze-marks from earlier wanderers, and the path cut a little wider along some of t...

    journal_title:Annals of biomedical engineering

    pub_type: 杂志文章,评审


    authors: Bassingthwaighte JB

    更新日期:1987-01-01 00:00:00

  • Stochasticity in membrane-localized "ligand-receptor-G protein" binding: consequences for leukocyte movement behavior.

    abstract::The signal that governs the chemotactic response of mammalian white blood cells and tissue cells arises from membrane-localized binding events involving chemotactic factor ligands and receptors and G proteins. Fluctuations in this signal have been traditionally attributed to significant "noise" in receptor-ligand bind...

    journal_title:Annals of biomedical engineering

    pub_type: 杂志文章


    authors: Moghe PV,Tranquillo RT

    更新日期:1995-05-01 00:00:00

  • Why, When, Who, What, How, and Where for Trainees Writing Literature Review Articles.

    abstract::Literature review articles provide a valuable mechanism for remaining informed amidst an ever-increasing body of scientific work. Condensing current advances into this disseminatable form is a critical activity for any research trainee. To systematize this multifaceted process, we present the "why, when, who, what, ho...

    journal_title:Annals of biomedical engineering

    pub_type: 杂志文章


    authors: Koons GL,Schenke-Layland K,Mikos AG

    更新日期:2019-11-01 00:00:00

  • Fluid dynamic assessment of three polymeric heart valves using particle image velocimetry.

    abstract::Polymeric heart valves have the potential to reduce thrombogenic complications associated with current mechanical valves and overcome fatigue-related problems experienced by bioprosthetic valves. In this paper we characterize the in vitro velocity and Reynolds Shear Stress (RSS) fields inside and downstream of three d...

    journal_title:Annals of biomedical engineering

    pub_type: 杂志文章


    authors: Leo HL,Dasi LP,Carberry J,Simon HA,Yoganathan AP

    更新日期:2006-06-01 00:00:00

  • Fabrication and mechanical evaluation of anatomically-inspired quasilaminate hydrogel structures with layer-specific formulations.

    abstract::A major tissue engineering challenge is the creation of multilaminate scaffolds with layer-specific mechanical properties representative of native tissues, such as heart valve leaflets, blood vessels, and cartilage. For this purpose, poly(ethylene glycol) diacrylate (PEGDA) hydrogels are attractive materials due to th...

    journal_title:Annals of biomedical engineering

    pub_type: 杂志文章


    authors: Tseng H,Cuchiara ML,Durst CA,Cuchiara MP,Lin CJ,West JL,Grande-Allen KJ

    更新日期:2013-02-01 00:00:00

  • Computer modeling of red blood cell rheology in the microcirculation: a brief overview.

    abstract::One of the major functions of the cardiovascular system is to deliver blood to the microcirculation where exchange of mass and energy can take place. In the present article, we will provide an overview of the state-of-the-art computational methods for modeling of red blood cell (RBC) rheology and dynamics in the micro...

    journal_title:Annals of biomedical engineering

    pub_type: 杂志文章


    authors: Cristini V,Kassab GS

    更新日期:2005-12-01 00:00:00

  • Evaluation of three animal models for concussion and serious brain injury.

    abstract::Three animal models were evaluated in this study involving head impacts of the rat, including the Marmarou drop-weight and two momentum-exchange techniques. In series 1, 36 Wistar rats were hit on the side of the free-moving head using Marmarou's 450 g impact mass at 4.4, 5.4, and 6.3 m/s. Head acceleration was measur...

    journal_title:Annals of biomedical engineering

    pub_type: 杂志文章


    authors: Viano DC,Hamberger A,Bolouri H,Säljö A

    更新日期:2012-01-01 00:00:00

  • A model for compound action potentials and currents in a nerve bundle. I: The forward calculation.

    abstract::We describe a model for the Compound Action Currents (CACs) and Compound Action Potentials (CAPs) produced by a peripheral nerve bundle in vitro. The Single Fiber Action Currents (SFACs) and the extracellular Single Fiber Action Potentials (SFAPs) are calculated using a generalized volume conduction model. Frequency-d...

    journal_title:Annals of biomedical engineering

    pub_type: 杂志文章


    authors: Wijesinghe RS,Gielen FL,Wikswo JP Jr

    更新日期:1991-01-01 00:00:00

  • Localized spatial discrimination of epicardial conduction paths after linear transformation of variant information.

    abstract::We present a method for the localized statistical discrimination of class populations based on the Karhunen-Loève and Fukunaga-Koontz transforms. These transforms provide features that model the variance of a sample distribution. The spatial series of a 196 channel epicardial electrogram recording from an arrythmogeni...

    journal_title:Annals of biomedical engineering

    pub_type: 杂志文章


    authors: Ciaccio EJ,Dunn SM,Akay M,Wit AL,Coromilas J,Costeas CA

    更新日期:1994-09-01 00:00:00

  • Estimating the parameter distributions of defibrillation shock efficacy curves in a large population.

    abstract::Defibrillation efficacy testing is a common procedure at defibrillator implantation, with the objective to ensure that each patient receives a system with adequate shock efficacy. In this study, defibrillation shock efficacy as a function of shock energy was modeled by a dose response relationship. The frequency distr...

    journal_title:Annals of biomedical engineering

    pub_type: 杂志文章


    authors: Smits K,Virag N

    更新日期:2010-04-01 00:00:00

  • Concussion Risk Between Individual Football Players: Survival Analysis of Recurrent Events and Non-events.

    abstract::Concussion tolerance and head impact exposure are highly variable among football players. Recent findings highlight that head impact data analyses need to be performed at the subject level. In this paper, we describe a method of characterizing concussion risk between individuals using a new survival analysis technique...

    journal_title:Annals of biomedical engineering

    pub_type: 杂志文章


    authors: Rowson S,Campolettano ET,Duma SM,Stemper B,Shah A,Harezlak J,Riggen L,Mihalik JP,Brooks A,Cameron KL,Svoboda SJ,Houston MN,McAllister T,Broglio S,McCrea M

    更新日期:2020-11-01 00:00:00

  • Superparamagnetic iron oxide is suitable to label tendon stem cells and track them in vivo with MR imaging.

    abstract::Tendon stem cells (TSCs) may be used to effectively repair or regenerate injured tendons. However, the fates of TSCs once implanted in vivo remain unclear. This study was aimed to determine the feasibility of labeling TSCs with super-paramagnetic iron oxide (SPIO) nano-particles to track TSCs in vivo using MRI. Rabbit...

    journal_title:Annals of biomedical engineering

    pub_type: 杂志文章


    authors: Yang Y,Zhang J,Qian Y,Dong S,Huang H,Boada FE,Fu FH,Wang JH

    更新日期:2013-10-01 00:00:00

  • Pulsatile flow in tubes of elliptic cross sections.

    abstract::The compression of blood vessels by surrounding tissue is an important problem in hemodynamics, most prominently in studies relating to the heart. In this study we consider a long tube of elliptic cross section as an idealization of the geometry of a compressed blood vessel. An exact solution of the governing equation...

    journal_title:Annals of biomedical engineering

    pub_type: 杂志文章


    authors: Haslam M,Zamir M

    更新日期:1998-09-01 00:00:00

  • Design and hydrodynamic evaluation of a novel pulsatile bioreactor for biologically active heart valves.

    abstract::Biologically active heart valves (tissue engineered and recellularized tissue-derived heart valves) have the potential to offer enhanced function when compared to current replacement value therapies since they can possibly remodel, and grow to meet the needs of the patient, and not require chronic medication. However,...

    journal_title:Annals of biomedical engineering

    pub_type: 杂志文章


    authors: Hildebrand DK,Wu ZJ,Mayer JE Jr,Sacks MS

    更新日期:2004-08-01 00:00:00

  • Matrix Stiffness Modulates Mesenchymal Stem Cell Sensitivity to Geometric Asymmetry Signals.

    abstract::Human stem cells hold significant potential for the treatment of various diseases. However, their use as a therapy is hampered because of limited understanding of the mechanisms by which they respond to environmental stimuli. Efforts to understand extracellular biophysical cues have demonstrated the critical roles of ...

    journal_title:Annals of biomedical engineering

    pub_type: 杂志文章


    authors: Piroli ME,Jabbarzadeh E

    更新日期:2018-06-01 00:00:00

  • Tracking of Drug Release and Material Fate for Naturally Derived Omega-3 Fatty Acid Biomaterials.

    abstract::In vitro and in vivo studies were conducted on omega-3 fatty acid-derived biomaterials to determine their utility as an implantable material for adhesion prevention following soft tissue hernia repair and as a means to allow for the local delivery of antimicrobial or antibiofilm agents. Naturally derived biomaterials ...

    journal_title:Annals of biomedical engineering

    pub_type: 杂志文章


    authors: Faucher KM,Artzi N,Beck M,Beckerman R,Moodie G,Albergo T,Conroy S,Dale A,Corbeil S,Martakos P,Edelman ER

    更新日期:2016-03-01 00:00:00

  • Effect of cell-cell interactions on the observable strength of adhesion of sheets of cells.

    abstract::Previous research in cellular adhesion has focused primarily on studying isolated cells under conditions where cells do not interact with each other. However, in vivo cells form sheets where both cell-substratum and cell-cell interactions contribute to the overall adhesive behavior. Our understanding of how cell-cell ...

    journal_title:Annals of biomedical engineering

    pub_type: 杂志文章


    authors: Peel MM,DiMilla PA

    更新日期:1999-03-01 00:00:00

  • Detergent-Based Decellularization of Bovine Carotid Arteries for Vascular Tissue Engineering.

    abstract::Vascular diseases are an increasing health issue, and common alloplastic, allogenic or autologous vascular grafts show frequent complications. The aim of this study is to develop an acellular, xenogenic bypass-graft from a bovine carotid artery (BAC) using detergent-based protocols. We compared decellularization with ...

    journal_title:Annals of biomedical engineering

    pub_type: 杂志文章


    authors: Daugs A,Hutzler B,Meinke M,Schmitz C,Lehmann N,Markhoff A,Bloch O

    更新日期:2017-11-01 00:00:00

  • An evaluation of population D-optimal designs via pharmacokinetic simulations.

    abstract::One goal of large scale clinical trials is to determine how a drug is processed by, and cleared from, the human body [i.e., its pharmacokinetic (PK) properties] and how these PK properties differ between individuals in a population (i.e., its population PK properties). Due to the high cost of these studies and the lim...

    journal_title:Annals of biomedical engineering

    pub_type: 杂志文章


    authors: Hooker AC,Foracchia M,Dodds MG,Vicini P

    更新日期:2003-01-01 00:00:00

  • Motile Dendritic Cells Sense and Respond to Substrate Geometry.

    abstract::Dendritic cell (DC) migration is required for efficient presentation of antigen to T cells and the initiation of an adaptive immune response. In spite of its importance, many aspects of DC migration have not been characterized. DCs encounter a variety of environments with different stiffness and geometry, but the effe...

    journal_title:Annals of biomedical engineering

    pub_type: 杂志文章


    authors: Bendell AC,Anderson N,Blumenthal D,Williamson EK,Chen CS,Burkhardt JK,Hammer DA

    更新日期:2018-09-01 00:00:00