Selective elution of HLA antigens and beta 2-microglobulin from human platelets by chloroquine diphosphate.

Abstract:

:To determine whether chloroquine can specifically elute HLA antigens and beta 2-microglobulin (beta 2-M) from the platelet surface, quantitative immunofluorescence flow cytometry and monoclonal antibodies were used to show that HLA antigens and beta 2-M were proportionally eluted from the platelet surface without affecting the membrane glycoproteins IIb and IIIa. Second, an autoradiogram of electrophoresed I125-labeled platelets showed that only beta 2-M but not other I125-labeled membrane proteins could be eluted. Although HLA antigens were poorly labeled by I125 and could not be detected on the autoradiogram, the eluted HLA antigens could be detected by anti-HLA monoclonal antibody and immunoblotting techniques. No loss of plasma membrane integrity was observed by transmission electron microscopy after chloroquine treatment of platelets. The results indicate that chloroquine selectively elutes HLA antigens and their noncovalently associated beta 2-M without affecting other integral platelet membrane proteins.

journal_name

Transfusion

journal_title

Transfusion

authors

Kao KJ

doi

10.1046/j.1537-2995.1988.28188127942.x

subject

Has Abstract

pub_date

1988-01-01 00:00:00

pages

14-7

issue

1

eissn

0041-1132

issn

1537-2995

journal_volume

28

pub_type

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