A large number of mature and functional dendritic cells can be efficiently generated from umbilical cord blood-derived mononuclear cells by a simple two-step culture method.

Abstract:

BACKGROUND:Advances in the past two decades in dendritic cell (DC) biology paved the way to exploit them as a promising tool in cancer immunotherapy. The prerequisite for DC vaccine preparations is large-scale in vitro generations of homogeneous, mature, and functional DCs. Frequent improvements are being made in the existing in vitro DC production protocols to achieve this goal. In our previous study we reported a large-scale generation of mature, functional DCs from umbilical cord blood (UCB) CD34+ cells. Here we report that this method can be used for the efficient generation of DCs from UCB mononuclear cells (MNCs) and thus the hematopoietic stem cell isolation step is not essential. STUDY DESIGN AND METHODS:MNCs or CD34+ cells isolated from the same cord blood (CB) samples were used for the generation of DCs. DCs were characterized for morphology, phenotype, and functional assays including antigen uptake, chemotaxis, and mixed leukocyte reaction. Similarly DCs generated from the MNCs of same fresh and frozen CB units were compared. RESULTS:The morphologic, phenotypic, and functional characterization of the DCs generated from various sets show that they were comparable in nature irrespective of the starting population used. CONCLUSION:We conclude that the CD34+ isolation step is not essential for the generation of mature, functional DCs and thus can be eliminated. More importantly, we show that DCs can be generated with equal efficiency from the MNCs of frozen CB units. Our culture method will be useful for exploiting the potential of UCB as an additional source for allogeneic DCs in the clinical settings.

journal_name

Transfusion

journal_title

Transfusion

authors

Balan S,Kale VP,Limaye LS

doi

10.1111/j.1537-2995.2010.02706.x

subject

Has Abstract

pub_date

2010-11-01 00:00:00

pages

2413-23

issue

11

eissn

0041-1132

issn

1537-2995

pii

TRF2706

journal_volume

50

pub_type

杂志文章
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    doi:10.1111/j.1537-2995.2011.03152.x

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  • HIV, HCV, and HBV incidence and residual risk in US blood donors before and after implementation of the 12-month deferral policy for men who have sex with men.

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    journal_title:Transfusion

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    doi:10.1111/trf.16250

    authors: Steele WR,Dodd RY,Notari EP,Haynes J,Anderson SA,Williams AE,Reik R,Kessler D,Custer B,Stramer SL,Transfusion-Transmissible Infections Monitoring System (TTIMS).

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  • A study of criteria for blood donor deferral.

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    pub_type: 杂志文章

    doi:10.1111/trf.13375

    authors: Avanzi MP,Oluwadara OE,Cushing MM,Mitchell ML,Fischer S,Mitchell WB

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    pub_type: 杂志文章

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  • The role of the paternal health history in cord blood banking.

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    pub_type: 杂志文章

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    authors: Askari S,Miller J,Clay M,Moran S,Chrysler G,McCullough J

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  • The minimum number of cord blood units needed for Koreans is 51,000.

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    journal_title:Transfusion

    pub_type: 杂志文章

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    authors: Yoon JH,Oh S,Shin S,Park JS,Roh EY,Song EY,Park MH,Han KS

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    journal_title:Transfusion

    pub_type: 杂志文章

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    authors: Lin-Chu M,Tsai SJ,Watanabe J,Nishioka K

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    pub_type: 杂志文章

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    authors: Reems JA,Fujita D,Tyler T,Moldwin R,Smith SD

    更新日期:1999-04-01 00:00:00

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    pub_type: 杂志文章,评审

    doi:10.1111/j.1537-2995.2012.03707.x

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    pub_type: 杂志文章

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    authors: Mertes PM,Tacquard C,Andreu G,Kientz D,Gross S,Malard L,Drouet C,Carlier M,Gachet C,Sandid I,Boudjedir K

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  • Red blood cell alloimmunization is associated with lower expression of FcγR1 on monocyte subsets in patients with sickle cell disease.

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    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.15463

    authors: Balbuena-Merle R,Curtis SA,Devine L,Gibb DR,Karafin MS,Luckey CJ,Tormey CA,Siddon AJ,Roberts JD,Hendrickson JE

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  • Evaluation of the putative role of C-C chemokines as protective factors of HIV-1 infection in seronegative hemophiliacs exposed to contaminated hemoderivatives.

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    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.2000.40040461.x

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  • "Naturally-occurring" anti-K1: possible association with mycobacterium infection.

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    journal_title:Transfusion

    pub_type: 杂志文章

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  • A new DEL variant caused by exon 8 deletion.

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    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2007.01199.x

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  • Single hospital experience of TRALI.

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    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.2003.00466.x

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  • Generation of kinins during preparation and storage of whole blood-derived platelet concentrates.

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    authors: Moreau ME,Thibault L,Désormeaux A,Chagnon M,Lemieux R,Robillard P,Marceau F,Colman RW,Lepage Y,Rivard GE,Adam A

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  • Multiple hemolytic transfusion reactions misinterpreted as severe vaso-occlusive crisis in a patient with sickle cell disease.

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    pub_type: 杂志文章

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  • Detection and identification of platelet antibodies using a sensitive multiplex assay system-platelet antibody bead array.

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    pub_type: 杂志文章

    doi:10.1111/trf.14122

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  • Iron deficiency anemia at admission for labor and delivery is associated with an increased risk for Cesarean section and adverse maternal and neonatal outcomes.

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    pub_type: 杂志文章

    doi:10.1111/trf.13252

    authors: Drukker L,Hants Y,Farkash R,Ruchlemer R,Samueloff A,Grisaru-Granovsky S

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  • Consequences and costs of noncompliance with iron chelation therapy in patients with transfusion-dependent thalassemia: a literature review.

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    pub_type: 杂志文章,评审

    doi:10.1111/j.1537-2995.2007.01416.x

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    更新日期:2007-10-01 00:00:00

  • First-time blood donors: demographic trends.

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    pub_type: 杂志文章

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    更新日期:2001-03-01 00:00:00

  • Human herpesvirus 8 transfusion transmission in Ghana, an endemic region of West Africa.

    abstract:BACKGROUND:Human herpesvirus 8 (HHV-8) seroprevalence ranges between less than 5% in Europe and North America and 50% to 70% in sub-Saharan Africa. Evidence of HHV-8 transfusion transmission is only indirect. We conducted a serologic (anti-HHV-8) and molecular (HHV-8 DNA) study of samples from paired donor-immunocompet...

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    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2012.03607.x

    authors: Gobbini F,Owusu-Ofori S,Marcelin AG,Candotti D,Allain JP

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    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.16028

    authors: Staples S,O'Callaghan C,Pavord S,Staves J,Murphy MF

    更新日期:2020-09-01 00:00:00

  • Enhanced ascertainment of microchimerism with real-time quantitative polymerase chain reaction amplification of insertion-deletion polymorphisms.

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    journal_title:Transfusion

    pub_type: 杂志文章,随机对照试验

    doi:10.1111/j.1537-2995.2006.00992.x

    authors: Lee TH,Chafets DM,Reed W,Wen L,Yang Y,Chen J,Utter GH,Owings JT,Busch MP

    更新日期:2006-11-01 00:00:00

  • Depletion of white cells from platelet concentrates with a new adsorption filter.

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    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1989.29589284140.x

    authors: Kickler TS,Bell W,Drew H,Pall D

    更新日期:1989-06-01 00:00:00

  • Preparation of white cell-depleted red cells for 42-day storage using an integral in-line filter.

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    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1989.29689318446.x

    authors: Davey RJ,Carmen RA,Simon TL,Nelson EJ,Leng BS,Chong C,Garcez RB,Sohmer PR

    更新日期:1989-07-01 00:00:00