Liquid storage at 4 degrees C of previously frozen red cells.

Abstract:

:Fresh human blood was collected in citrate-phosphate-dextrose, frozen by a high-glycerol technique, and stored at -80 degrees C. The red cells were thawed, deglycerolized, and resuspended in a final wash solution, ADSOL (Fenwal Laboratories), or an additive solution (AS) containing glucose, adenine, mannitol, and phosphate. The cells were then stored at 4 to 6 degrees C for 21 days and assayed weekly for adenosine triphosphate and 2,3 diphosphoglycerate, pH, glucose use, and lysis. AS and, to a lesser extent, ADSOL produced metabolic profiles similar to or better than profiles of cells not frozen and stored in commercially available additive solutions. AS offers a potential post-thaw preservative solution for red cells that would greatly increase the flexibility and reduce the expense of using frozen blood. A sterile post-thaw storage capability will make the stockpiling of frozen red cells a practical concept for both military and civilian blood banks.

journal_name

Transfusion

journal_title

Transfusion

authors

Moore GL,Ledford ME,Mathewson PJ,Hankins DJ

doi

10.1046/j.1537-2995.1987.27688071704.x

subject

Has Abstract

pub_date

1987-11-01 00:00:00

pages

496-8

issue

6

eissn

0041-1132

issn

1537-2995

journal_volume

27

pub_type

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