Abstract:
BACKGROUND:As clinical diagnosis of heparin-associated thrombocytopenia (HAT) is often difficult, confirmation by sensitive laboratory assays is desirable. STUDY DESIGN AND METHODS:The sensitivity of the heparin-induced platelet activation (HIPA) test and the platelet aggregation test (PAT) was prospectively compared by using the sera of 209 patients with the putative diagnosis of HAT. Both assays were performed concomitantly with platelets of the same four donors using a different combination of donors from day to day. Further, all sera were assessed with a platelet factor 4 (PF4)/heparin enzyme-linked immunosorbent assay (ELISA). RESULTS:Positive results were obtained with 33 percent of sera in the PF4/heparin ELISA, with 33.5 percent of sera in the HIPA test, and with 11.5 percent of sera in the PAT. The PF4/heparin ELISA and the HIPA test showed no difference in sensitivity (p = 0.27 by McNemar's test) and were more sensitive than PAT (p < 10(-8) by McNemar's test). However, they recognized different patient cohorts. Nine HIPA-indeterminate and 12 HIPA-negative sera were positive in the PF4/heparin ELISA. Eight of the nine indeterminate sera caused platelet activation with high heparin concentrations in the HIPA test. Eleven of the 12 negative sera contained no IgG, but 9 contained IgM and 2 contained IgA HAT antibodies. Four sera that were indeterminate in the PF4/heparin ELISA and 18 sera that were negative were positive in the HIPA test. None of the sera that were positive in the PAT was missed in the HIPA test, but two of those were negative in the PF4/heparin ELISA. All sera were assessed with four low-molecular-weight heparins and a low-molecular-weight heparinoid in the HIPA test with platelets from the same four donors. Low-molecular-weight heparin caused platelet activation with positive sera in 98 percent of tests, and the heparinoid did so in 10 percent; in a further 12.8 percent, crossreactivity to the low-molecular-weight heparinoid could not be excluded. CONCLUSION:The majority of HAT antibodies react with a PF4/heparin complex, but there is strong evidence that other antigens are involved in some patients. The HIPA test and the PF4/heparin ELISA are sensitive for diagnosing HAT, and they complement one another.
journal_name
Transfusionjournal_title
Transfusionauthors
Greinacher A,Amiral J,Dummel V,Vissac A,Kiefel V,Mueller-Eckhardt Cdoi
10.1046/j.1537-2995.1994.34594249047.xsubject
Has Abstractpub_date
1994-05-01 00:00:00pages
381-5issue
5eissn
0041-1132issn
1537-2995journal_volume
34pub_type
杂志文章相关文献
TRANSFUSION文献大全abstract::Simple methods have been developed for adding and removing glycerol during freeze-preservation with 20 per cent W/V glycerol at minus 150 C, or with 40 per cent W/V glycerol at minus 80 C. A one-step method with a 35 per cent W/V glycerol solution is used to prepare 20 per cent W/V glycerolized red blood cells, and a ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1975.15375160354.x
更新日期:1975-05-01 00:00:00
abstract:BACKGROUND:Studies analyzing motivation factors that lead to blood donation have found altruism to be the primary motivation factor; however, social capital has not been analyzed in this context. Our study examines the association between motivation factors (altruism, self-interest, and response to direct appeal) and s...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2012.03887.x
更新日期:2013-06-01 00:00:00
abstract:BACKGROUND:Human T-cell lymphoma/leukemia viruses types I and II (HTLV-I and HTLV-II) are related exogenous human retroviruses. The former is definitely pathogenic while disease association with the latter is unclear. There are two subtypes of HTLV-II, A and B. Currently, enzyme-linked immunosorbent assays (ELISAs) bas...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1997.37197176951.x
更新日期:1997-01-01 00:00:00
abstract:BACKGROUND:The need for blood products is constant and unremitting, yet only a small percentage of eligible individuals answer the appeal to donate. Further, most new donors never return to provide a repeat donation. The ongoing need to attract and retain new donors has led to the examination of psychosocial factors th...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2007.01236.x
更新日期:2007-06-01 00:00:00
abstract::A non-diethylhexyl phthalate (DEHP)-plasticized blood bag for 5-day storage of random-donor platelet concentrates has been developed. The plastic bag is composed of polyvinylchloride plastic with a butyryl trihexyl citrate plasticizer. The suitability of this plastic for the storage of platelet concentrates for use in...
journal_title:Transfusion
pub_type: 临床试验,杂志文章
doi:10.1046/j.1537-2995.1992.32893032101.x
更新日期:1992-10-01 00:00:00
abstract::Autologous blood donors (ABDs) have been reported to have favorable attitudes toward returning as homologous blood donors (HBDs), but the frequency of return has not been well documented. ABDs eligible by history to be HBDs were followed at one blood center: 255 donating for elective surgery and 234 donating during pr...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1991.31291142941.x
更新日期:1991-02-01 00:00:00
abstract::Newly prepared platelet concentrates (PC) and platelet poor plasma (PPP) stored at either room temperature (RT) or 4 C were analyzed for ammonia content initially, and after storage at 24, 48, and 72 hours. These studies demonstrate that ammonia rapidly accumulates in PC stored at RT. This accumulation appears to be t...
journal_title:Transfusion
pub_type: 杂志文章
doi:
更新日期:1981-01-01 00:00:00
abstract:BACKGROUND:There are no reports in the transfusion medicine literature that describe adverse reactions and donor arm injuries after whole-blood donation based on solicited information. STUDY DESIGN AND METHODS:The present study solicited adverse reaction and donor arm injury information from 1000 randomly selected who...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.2003.00368.x
更新日期:2003-05-01 00:00:00
abstract:BACKGROUND:Novel gel centrifugation test (GCT) cards were evaluated with respect to their ability to estimate the quantity of IgG on RBCs and the determination of the IgG subclasses IgG1 and IgG3. STUDY DESIGN AND METHODS:In 65 patients with a positive DAT, the amount of IgG-gamma-, IgG1, and IgG3 on RBCs was examined...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.2002.00076.x
更新日期:2002-05-01 00:00:00
abstract:BACKGROUND:This multicenter study was performed to evaluate the efficiency of a multiplex individual-donation nucleic acid amplification technology (ID-NAT) and discriminatory testing algorithm for detecting hepatitis B virus (HBV) infection in Chinese blood donors. STUDY DESIGN AND METHODS:A total of 1,205,796 hepati...
journal_title:Transfusion
pub_type: 杂志文章,多中心研究
doi:10.1111/trf.13135
更新日期:2015-09-01 00:00:00
abstract:BACKGROUND:There has been controversy over whether HLA alloimmunization is a risk factor for platelet (PLT) transfusion refractoriness (PTR) in hematopoietic peripheral blood progenitor cell transplantation (HPBPCT). STUDY DESIGN AND METHODS:Reported here is a boy with relapsed leukemia who developed fatal PTR after a...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2007.01109.x
更新日期:2007-02-01 00:00:00
abstract::Red cells from a Mexican blood donor had depressed H, IF, ID, i, Sda, Sdx, D, LW, Dib, Vel and JMH antigens. A wide range of other blood group antigens had normal activity. The unusual red cell phenotype may be caused by a membrane anomaly that alters orientation of certain cell surface components. The specificities o...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1983.23183147309.x
更新日期:1983-01-01 00:00:00
abstract:BACKGROUND:Since their description in the 1970s, anti-Hr(B) (antibody against a high-prevalence Rh antigen) and anti-hr(B) (anti-e-like antibody) are still a subject of debate about representing two aspects of a global immune response or being two independent antibodies. STUDY DESIGN AND METHODS:The aim of this study ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2009.02307.x
更新日期:2009-11-01 00:00:00
abstract:BACKGROUND:Trypanosoma cruzi, the protozoan cause of Chagas disease, causes life-long infection and is easily transmitted by blood transfusion. Our goals were to determine the prevalence of Chagas disease among donors in five Mexican blood banks, to look for evidence of transmission of T. cruzi by transfusion, and to e...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2006.00715.x
更新日期:2006-02-01 00:00:00
abstract:BACKGROUND:Transfusion-induced serum sickness reactions are rarely reported in the literature. The Type III hypersensitivity reaction to heterologous proteins involves deposition of complement and immune complexes in small vessel walls resulting in a leukocytoclastic vasculitis. A case of a multiply transfused patient ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2008.01956.x
更新日期:2009-02-01 00:00:00
abstract:BACKGROUND:The characterization of microchimerism (MC) by gene amplification has been limited by few allogeneic markers, ascertainment bias, and assay analytic performance. To address this, a panel of 12 MC assays based on insertion-deletion (InDel) polymorphisms had been optimized. STUDY DESIGN AND METHODS:The InDel ...
journal_title:Transfusion
pub_type: 杂志文章,随机对照试验
doi:10.1111/j.1537-2995.2006.00992.x
更新日期:2006-11-01 00:00:00
abstract:BACKGROUND:Whole blood donors are at risk of becoming iron deficient. To monitor iron stores, Sanquin implemented a new deferral policy based on ferritin levels, in addition to the traditional hemoglobin measurements. METHODS:Ferritin levels are determined in every fifth donation, as well as in all first-time donors. ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.15906
更新日期:2020-08-01 00:00:00
abstract:BACKGROUND:The decision to transfuse red blood cells in surgical patients should be based on multiple clinical variables, rather than on isolated hemoglobin (Hb) measurements alone. An important but often unrecognized clinical variable is the postoperative downward drift in Hb concentration (Hb drift), but the etiology...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.12491
更新日期:2014-06-01 00:00:00
abstract::A stable mouse hybridoma line secreting monoclonal anti-C3d antibody (NBTS/BRIC 8) was produced. Ascitic fluid containing BRIC 8 can be used at a final dilution of 1 to 5000 to provide an excellent antiglobulin reagent when mixed with a conventional polyclonal rabbit anti-human IgG serum. ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1985.25385219912.x
更新日期:1985-05-01 00:00:00
abstract:BACKGROUND:Flow cytometric measurement of CD34+ events is used to ensure the quality of human progenitor cell grafts. This study was conducted to evaluate whether the spiking of routine samples from peripheral blood and apheresis products with CD34+ positive controls is feasible. STUDY DESIGN AND METHODS:A total of 42...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2011.03259.x
更新日期:2012-02-01 00:00:00
abstract:BACKGROUND:Understanding factors that impact tissue oxygen extraction may guide red blood cell (RBC) transfusion decision making in preterm infants. Our objective was to assess the influence of enteral feeding and anemia on cerebral and mesenteric oxygen saturation (Csat and Msat) and fractional tissue oxygen extractio...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.15680
更新日期:2020-03-01 00:00:00
abstract:BACKGROUND:Transfusion of the incorrect blood component is a frequent serious incident associated with transfusion and often involves misidentification of the patient and/or the unit of blood. The objective of this study was to assess the effect of a simple intervention designed to improve performance of the bedside ch...
journal_title:Transfusion
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1111/j.1537-2995.2007.01189.x
更新日期:2007-05-01 00:00:00
abstract:BACKGROUND:Almost 2 percent of murine blood red blood cells (RBCs) are destroyed each day and are replaced by fresh RBCs generated through the process of erythropoiesis. RBCs to be destroyed are phagocytosed by macrophages in the reticuloendothelial system, especially in the spleen. CD47 molecules on RBCs may regulate ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2007.01348.x
更新日期:2007-09-01 00:00:00
abstract:BACKGROUND:Babesia microti, the most frequently implicated pathogen in transfusion-transmitted babesiosis, is widely endemic in the Northeast and upper Midwestern United States. High seroprevalence in endemic areas limits antibody-based donor screening. A high-performance molecular test is needed to identify donors in ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.12098
更新日期:2013-10-01 00:00:00
abstract:BACKGROUND:Some jurisdictions require leukoreduction of cellular blood components. The only whole blood collection set with a platelet-saving filter uses citrate-phosphate-dextrose (CPD) as storage solution. Substituting CPD with citrate-phosphate-dextrose-adenine (CPDA-1) increases shelf life from 21 to 35 days. This ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.15748
更新日期:2020-05-01 00:00:00
abstract:BACKGROUND:The quality of platelets (PLTs) stored in PLT additive solution (PAS) is dependent on the type and proportion of the used PAS. No data are available as to whether a different hold time before the addition of PAS to hyperconcentrated PLT suspensions has an impact on PLT quality. The in vitro quality between s...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2006.00826.x
更新日期:2006-06-01 00:00:00
abstract:BACKGROUND:The Joint Commission on the Accreditation of Healthcare Organizations requires that hospitals have a planned approach to systematically collect data on processes related to the use, ordering, and administering of blood components. This study describes how a comprehensive blood-administering assessment progra...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2004.03117.x
更新日期:2004-09-01 00:00:00
abstract::Two monoclonal antibodies (MoAbs), BRIC 66 (IgM) and BRIC 111 (IgG1), were produced by immunizing mice with ovarian cyst blood group A1 glycoprotein and Tn red cells (RBCs), respectively. Their specificities were determined by inhibitions using Tn sialoglycoproteins (SGPs), mucins (armadillo [ASG] and ovine [OSG] subm...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1991.31291142945.x
更新日期:1991-02-01 00:00:00
abstract:BACKGROUND:Previously, we evaluated the Mirasol pathogen reduction technology (PRT) system on platelet (PLT) function before resuspension. We now evaluated this system in the presence of PLT additive solution (PAS). STUDY DESIGN AND METHODS:Double-dose PLTs (n = 15) were generated using a commercially available aphere...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2011.03323.x
更新日期:2012-03-01 00:00:00
abstract:BACKGROUND:This study evaluated the effect of routine, uncontrolled, Israeli field storage conditions on the stability and efficacy of Lyo-Plas N freeze-dried plasma (FDP). We evaluated clotting factors V, VIII, and XI; proteins S and C; fibrinogen; partial thromboplastin time (PTT); antithrombin III (ATIII); von Wille...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.15533
更新日期:2019-11-01 00:00:00