Development of a real-time polymerase chain reaction assay for sensitive detection and quantitation of Babesia microti infection.

Abstract:

BACKGROUND:Babesia microti, the most frequently implicated pathogen in transfusion-transmitted babesiosis, is widely endemic in the Northeast and upper Midwestern United States. High seroprevalence in endemic areas limits antibody-based donor screening. A high-performance molecular test is needed to identify donors in the preseroconversion window phase as well as to discriminate past serologic exposure with parasite clearance from continued parasitemia. STUDY DESIGN AND METHODS:Frozen Babesia-spiked whole blood was microcentrifuged, and the supernatant transferred and microcentrifuged again to concentrate the parasite. The DNA was extracted and amplified using real-time polymerase chain reaction (PCR) using Babesia-specific primers. The assay was employed in three series of experiments: 1) a validation and optimization spiking experiment, 2) a blinded serial dilution probit analysis to determine the limit of detection, and 3) evaluation of two blinded panels of clinical samples from possible babesiosis cases. RESULTS:At a decreasing inoculum of 445, 44.5, and 4.45 copies/mL, the assay had positive rates of 100, 97.5, and 81%, respectively. The blinded probit analysis demonstrated a detection rate of 95 and 50% at 12.92 and 1.52 parasites/2 mL of whole blood, respectively. Evaluation of clinical samples showed 13 of 21 samples to be positive, with a range of 85 to 4.8 million parasites/mL. There were no positives detected among 48 healthy donors CONCLUSION:We have developed a highly sensitive and specific, quantitative real-time PCR-based assay for detection of B. microti that could have a useful role in blood screening. It can also be employed broadly to understand Babesia epidemiology, disease pathogenesis, and host immunology.

journal_name

Transfusion

journal_title

Transfusion

authors

Bloch EM,Lee TH,Krause PJ,Telford SR 3rd,Montalvo L,Chafets D,Usmani-Brown S,Lepore TJ,Busch MP

doi

10.1111/trf.12098

subject

Has Abstract

pub_date

2013-10-01 00:00:00

pages

2299-306

issue

10

eissn

0041-1132

issn

1537-2995

journal_volume

53

pub_type

杂志文章
  • An automated motivational interview promotes donation intention and self-efficacy among experienced whole blood donors.

    abstract:BACKGROUND:Interventions intended to motivate donors to return can be costly and time consuming. The current study examined the effect of a Web-based automated interview, informed by motivational interviewing and self-determination theory, on donor intention, motivation, and behavior in a sample of highly experienced d...

    journal_title:Transfusion

    pub_type: 杂志文章,随机对照试验

    doi:10.1111/trf.15402

    authors: Livitz IE,France CR,France JL,Fox KR,Ankawi B,Slepian PM,Kessler DA,Rebosa M,Shaz BH

    更新日期:2019-09-01 00:00:00

  • Removal of citrate from PAS-III additive solution improves functional and biochemical characteristics of buffy-coat platelet concentrates stored for 7 days, with or without INTERCEPT pathogen reduction.

    abstract:BACKGROUND:Deterioration in quality of platelet concentrates (PCs) during storage results from the appearance of storage lesions affecting the hemostatic functions and posttransfusion survival of platelets. These lesions depend on the preparation and pathogen inactivation methods used, duration of storage, and platelet...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.16280

    authors: Isola H,Ravanat C,Rudwill F,Pongerard A,Haas D,Eckly A,Gachet C,Hechler B

    更新日期:2021-02-01 00:00:00

  • A case for stocking O D+ red blood cells in emergency room trauma bays.

    abstract:BACKGROUND:AABB Standard 5.27 requires transfusion services to have a process for urgent release of blood before completion of compatibility testing. Our institution endorses a policy for the emergency release of group O, D+ red blood cells (RBC; O+ RBC) to males and females at least 50 years of age. Our emergency depa...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.12925

    authors: Meyer E,Uhl L

    更新日期:2015-04-01 00:00:00

  • Selective elution of HLA antigens and beta 2-microglobulin from human platelets by chloroquine diphosphate.

    abstract::To determine whether chloroquine can specifically elute HLA antigens and beta 2-microglobulin (beta 2-M) from the platelet surface, quantitative immunofluorescence flow cytometry and monoclonal antibodies were used to show that HLA antigens and beta 2-M were proportionally eluted from the platelet surface without affe...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1988.28188127942.x

    authors: Kao KJ

    更新日期:1988-01-01 00:00:00

  • Red cell use during cesarean delivery.

    abstract::The transfusion of red cells (RBCs) was analyzed over a 4-year period (1984-1987), during which 9596 cesarean deliveries were performed. A total of 336 patients were identified as receiving RBC transfusions during or after cesarean delivery; 747 units of RBCs were administered. The overall incidence of transfusion in ...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1991.31191096177.x

    authors: Camann WR,Datta S

    更新日期:1991-01-01 00:00:00

  • Identification and classification of the causes of events in transfusion medicine.

    abstract:BACKGROUND:Transfusion medicine lacks a standard method for the systematic collection and analysis of event reports. Review of event reports from the Food and Drug Administration (FDA) showed a relative paucity of information on event causation. Thus, a causal analysis method was developed as part of a prototype Medica...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1998.38111299056319.x

    authors: Kaplan HS,Battles JB,Van der Schaaf TW,Shea CE,Mercer SQ

    更新日期:1998-11-01 00:00:00

  • Social support attenuates presyncopal reactions to blood donation.

    abstract:BACKGROUND:The experience of unpleasant blood donation reactions (e.g., dizziness, nausea, and fainting) has been linked to negative attitudes about donation and decreased likelihood of repeat donation. Consequently, interventions to reduce the adverse effects of blood donation are important and likely to increase dono...

    journal_title:Transfusion

    pub_type: 杂志文章,随机对照试验

    doi:10.1111/j.1537-2995.2008.02057.x

    authors: Hanson SA,France CR

    更新日期:2009-05-01 00:00:00

  • A possible role for the production of multiple HLA antibodies in fatal platelet transfusion refractoriness after peripheral blood progenitor cell transplantation from the mother in a patient with relapsed leukemia.

    abstract:BACKGROUND:There has been controversy over whether HLA alloimmunization is a risk factor for platelet (PLT) transfusion refractoriness (PTR) in hematopoietic peripheral blood progenitor cell transplantation (HPBPCT). STUDY DESIGN AND METHODS:Reported here is a boy with relapsed leukemia who developed fatal PTR after a...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2007.01109.x

    authors: Nakazawa Y,Saito S,Hasegawa Y,Yanagisawa R,Sakashita K,Kamijo T,Miyazaki T,Sato S,Ikeda H,Ikebuchi K,Koike K

    更新日期:2007-02-01 00:00:00

  • Transfusion and component characteristics are not associated with allergic transfusion reactions to apheresis platelets.

    abstract:BACKGROUND:Transfusion-related characteristics have been hypothesized to cause allergic transfusion reactions (ATRs) but they have not been thoroughly studied. The primary objective of this study is to evaluate the associations of infusion rate, infusion volume, ABO mismatching, component age, and pretransfusion medica...

    journal_title:Transfusion

    pub_type: 临床试验,杂志文章

    doi:10.1111/trf.12852

    authors: Savage WJ,Tobian AA,Savage JH,Hamilton RG,Borge PD,Kaufman RM,Ness PM

    更新日期:2015-02-01 00:00:00

  • Increased risk of volume overload with plasma compared with four-factor prothrombin complex concentrate for urgent vitamin K antagonist reversal.

    abstract:BACKGROUND:Plasma is commonly used for vitamin K antagonist (VKA) reversal, but observational studies suggest that it is associated with transfusion-related adverse reactions (e.g., volume overload). However, this issue has not previously been addressed in a randomized controlled trial (RCT). STUDY DESIGN AND METHODS:...

    journal_title:Transfusion

    pub_type: 杂志文章,随机对照试验

    doi:10.1111/trf.13191

    authors: Refaai MA,Goldstein JN,Lee ML,Durn BL,Milling TJ Jr,Sarode R

    更新日期:2015-11-01 00:00:00

  • Red blood cell (RBC) survival determined in humans using RBCs labeled at multiple biotin densities.

    abstract:BACKGROUND:Safe, accurate methods permitting simultaneous and/or repeated measurement of red blood cell (RBC) survival (RCS) are important to investigate pathophysiology and therapy of anemia. Methods using chromium 51 ((51) Cr)-labeled RBCs are unacceptable for infants, children, and pregnant women. We report RCS meas...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2010.02926.x

    authors: Mock DM,Matthews NI,Zhu S,Strauss RG,Schmidt RL,Nalbant D,Cress GA,Widness JA

    更新日期:2011-05-01 00:00:00

  • Resolution by erythrocytapheresis of the exposure of an Rh-negative person to Rh-positive cells: an alternative treatment.

    abstract::Acute trauma with severe bleeding caused the unavoidable transfusion of Rh-positive red cells (RBCs) to a young, childless, Rh-negative woman during surgery. Of the 10 units of RBCs given, 6 were Rh positive. An alternative treatment for large-volume exposure involves the removal of some of the Rh-positive cells trans...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1993.33693296819.x

    authors: Werch J,Todd C

    更新日期:1993-06-01 00:00:00

  • Stability after thawing of RBCs frozen with the high- and low-glycerol method.

    abstract:BACKGROUND:RBCs can be frozen with either the high-glycerol method (HGM) or the low-glycerol method (LGM). To date, the use of frozen RBCs is hampered by a 24-hour outdating period after thawing. A closed washing system (ACP 215) may solve this problem. STUDY DESIGN AND METHODS:We compared the effects of high- (40%) a...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.2003.00293.x

    authors: Lelkens CC,Noorman F,Koning JG,Truijens-de Lange R,Stekkinger PS,Bakker JC,Lagerberg JW,Brand A,Verhoeven AJ

    更新日期:2003-02-01 00:00:00

  • A correlation between growth rate, apoptosis, and tumor necrosis factor-α in umbilical cord blood cells infected with two strains of Mycobacterium tuberculosis.

    abstract:BACKGROUND:Using umbilical cord blood (UCB) cells, it was demonstrated that three virulent isolates, two highly transmissible clinical isolates and the virulent laboratory strain, demonstrated rapid growth in the UCB cells, which was significantly faster than the growth rate observed for a unique isolate. There was als...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2009.02187.x

    authors: Theus JW,Justus RS,Theus SA

    更新日期:2009-08-01 00:00:00

  • Analysis of platelet-derived extracellular vesicles in plateletpheresis concentrates: a multicenter study.

    abstract:BACKGROUND:Routine quantification of platelet-derived extracellular vesicles (PL-EVs) may be useful in the quality control (QC) of platelet concentrates (PCs). The aim of this multicenter study was to establish and validate a consensus protocol for the standardized PL-EV quantification using conventional flow cytometer...

    journal_title:Transfusion

    pub_type: 杂志文章,多中心研究

    doi:10.1111/trf.14109

    authors: Black A,Orsó E,Kelsch R,Pereira M,Kamhieh-Milz J,Salama A,Fischer MB,Meyer E,Frey BM,Schmitz G

    更新日期:2017-06-01 00:00:00

  • Length of storage and in vitro immunomodulation induced by prestorage leukoreduced red blood cells.

    abstract:BACKGROUND:The relationship between length of storage of red blood cell (RBC) units and biochemical changes has been well studied, but little is known about the progression of cellular immunomodulative properties in blood recipients. This study aims to quantify in vitro T-cell activation and cytokine release by white b...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2009.02319.x

    authors: Karam O,Tucci M,Toledano BJ,Robitaille N,Cousineau J,Thibault L,Lacroix J,Le Deist F

    更新日期:2009-11-01 00:00:00

  • Donor and nondonor motivations.

    abstract::In an attempt to gain insight into the motivations of blood donors and nondonors, two paper and pencil questionnaires were developed and mailed to approximately 7,000 individuals. In response, 1,429 nondonors and 200 donors completed and returned usable questionnaires. Among donors, awareness of the need for blood, al...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1976.16276155116.x

    authors: Leibrecht BC,Hogan JM,Luz GA,Tobias KI

    更新日期:1976-03-01 00:00:00

  • The effects of dilution on the outcome of pooled plasma testing with HIV type 1 (HIV-1) RNA genome amplification as compared to the outcome of individual-unit testing with other HIV-1 markers.

    abstract:BACKGROUND:Proposed testing of large plasma pools with genome amplification technology (GAT) for detection of transfusion-transmissible viruses may have unanticipated complications not associated with individual unit testing. One such potential complication, the effect of dilution resulting from pool formation, was the...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1998.38598297216.x

    authors: Kemper M,Witt D,Madsen T,Kuramoto K,Holland P

    更新日期:1998-05-01 00:00:00

  • The controversy of unmodified versus citrated blood transfusion in the early 20th century.

    abstract::Many strides were made in the field of blood transfusion before and during World War I. However, it was not until after the war that one of the most exciting and controversial episodes in the field of blood transfusion occurred. Review of the literature revealed the 1920s to be a tumultuous era filled with conflicting...

    journal_title:Transfusion

    pub_type: 历史文章,杂志文章

    doi:10.1046/j.1537-2995.1984.24585017829.x

    authors: Wain SL

    更新日期:1984-09-01 00:00:00

  • An evaluation of several blood transfusion filters.

    abstract::Several blood transfusion filters designed to remove microemboli were evaluated for filtration and flow characteristics, as well as for hemolysis and for release of particulate debris from the filter. The Dacron wool filter, a depth filter, was superior at removing microemboli measured with an electronic particle size...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1977.17678075651.x

    authors: Kennedy PS,Solis RT,Scott MA,Wilson RK

    更新日期:1977-11-01 00:00:00

  • Th activation of maternal and cord blood.

    abstract::Th activation of red cells is characterized by agglutination with the peanut lectin from Arachis hypogaea and is diminished by treatment with proteolytic enzymes. The first cases of Th activation were associated with bacterial infections. More recently, a high incidence of Th activation in congenital hypoplastic anemi...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1989.29789369684.x

    authors: Wahl CM,Herman JH,Shirey RS,Kickler TS,Ness PM

    更新日期:1989-09-01 00:00:00

  • Red blood cell minor antigen mismatches during chronic transfusion therapy for sickle cell anemia.

    abstract:BACKGROUND:Red blood cell (RBC) alloimmunization occurs at a high frequency in sickle cell anemia (SCA) despite serologic matching for Rh (C/c, E/e) and K antigens. RBC minor antigen genotyping allows for prediction of antigens and RH variants that may lead to alloimmunization. STUDY DESIGN AND METHODS:RBC antigen gen...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.14282

    authors: Yee MEM,Josephson CD,Winkler AM,Webb J,Luban NLC,Leong T,Stowell SR,Fasano RM

    更新日期:2017-11-01 00:00:00

  • The survival and function of baboon red blood cells, platelets, and plasma proteins: a review of the experience from 1972 to 2002 at the Naval Blood Research Laboratory, Boston, Massachusetts.

    abstract::The studies reported in this monograph were performed between 1972 and 2002 when it was possible to study healthy male and female baboons. A colony of baboons was maintained for 30 years without any adverse events observed in these baboons in the numerous studies that were performed. These protocols were reviewed and ...

    journal_title:Transfusion

    pub_type: 杂志文章,评审

    doi:10.1111/j.1537-2995.2006.00922.x

    authors: Valeri CR,Ragno G

    更新日期:2006-08-01 00:00:00

  • An immediate, allergic skin reaction to aprotinin after reexposure to fibrin sealant.

    abstract:BACKGROUND:The safety of fibrin tissue adhesives has been a concern since they entered wide clinical application. Most commercially available kits contain the proteolytic inhibitor, aprotinin, to stabilize the fibrin clot. A bovine protein, this substance has an allergenic potency. CASE REPORT:This case report present...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.2000.40030302.x

    authors: Beierlein W,Scheule AM,Antoniadis G,Braun C,Schosser R

    更新日期:2000-03-01 00:00:00

  • Freeze-dried plasma stability under prehospital field conditions.

    abstract:BACKGROUND:This study evaluated the effect of routine, uncontrolled, Israeli field storage conditions on the stability and efficacy of Lyo-Plas N freeze-dried plasma (FDP). We evaluated clotting factors V, VIII, and XI; proteins S and C; fibrinogen; partial thromboplastin time (PTT); antithrombin III (ATIII); von Wille...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.15533

    authors: Zur M,Glassberg E,Gorenbein P,Epstein E,Eisenkraft A,Misgav M,Avramovich E

    更新日期:2019-11-01 00:00:00

  • Prevalence of the newly described human circovirus, TTV, in United States blood donors.

    abstract:BACKGROUND:A novel nonenveloped single-stranded circular DNA virus (TTV) was recently identified. The prevalence of TTV in blood donors in the United States is, however, still unclear. STUDY DESIGN AND METHODS:Viral DNA was detected in US blood donors from five cities by using two sets of TTV primers: NG059/NG061/NG06...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.2000.40020245.x

    authors: Handa A,Dickstein B,Young NS,Brown KE

    更新日期:2000-02-01 00:00:00

  • Human T-lymphotropic virus type I and type II infections and correlation with risk factors in blood donors from São Paulo, Brazil.

    abstract:BACKGROUND:Little is known about the prevalence of and risk factors for human T-lymphotropic virus type I and type II (HTLV-I, HTLV-II) infections in Brazil. STUDY DESIGN AND METHODS:Sera from 17,063 healthy Brazilian donors were screened by enzyme-linked immunosorbent assay for antibody to HTLV-I/II between August 19...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1995.35395184284.x

    authors: Ferreira Júnior OC,Vaz RS,Carvalho MB,Guerra C,Fabron AL,Rosemblit J,Hamerschlak N

    更新日期:1995-03-01 00:00:00

  • A new radioimmunoassay for the detection of small amounts of white cells and platelets in red cell concentrates: implications for blood transfusion.

    abstract::In the procedure for quality control of red cell concentrates, made white cell (WBC)-poor by filtration, the particle-counting technique was found to be insufficiently sensitive in detecting the remaining WBCs and platelets. Therefore, direct radioimmunoassays were developed using murine monoclonal antibodies specific...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1990.30290162896.x

    authors: Webster MR,Prins HK,Huisman JG

    更新日期:1990-02-01 00:00:00

  • Return of autologous blood donors as homologous blood donors.

    abstract::Autologous blood donors (ABDs) have been reported to have favorable attitudes toward returning as homologous blood donors (HBDs), but the frequency of return has not been well documented. ABDs eligible by history to be HBDs were followed at one blood center: 255 donating for elective surgery and 234 donating during pr...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1991.31291142941.x

    authors: McVay PA,Fung HC,Toy PT

    更新日期:1991-02-01 00:00:00

  • A review of blood donor motivation and recruitment.

    abstract::Sixty English-language articles on the motivation and recruitment of blood donors and nondonors are reviewed and evaluated. Apparently researchers have been finding essentially the same results for approximately twenty years. Motivations to donate are: altruism/humanitarian, personal or family credit, social pressure,...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1977.17277151916.x

    authors: Oswalt RM

    更新日期:1977-03-01 00:00:00