The effects of dilution on the outcome of pooled plasma testing with HIV type 1 (HIV-1) RNA genome amplification as compared to the outcome of individual-unit testing with other HIV-1 markers.


BACKGROUND:Proposed testing of large plasma pools with genome amplification technology (GAT) for detection of transfusion-transmissible viruses may have unanticipated complications not associated with individual unit testing. One such potential complication, the effect of dilution resulting from pool formation, was the subject of the present study. STUDY DESIGN AND METHODS:Specimens from three plasma donor HIV type 1 (HIV-1) seroconversion panels were tested with a quantitative HIV-1 RNA GAT assay (lower detection limit, 400 copies). GAT results were compared to HIV-1 p24 antigen and anti-HIV-1/2 enzyme immunoassay results. Effects of dilution on the detection of GAT-positive panel specimens were assessed by terminal dilution with pooled volunteer-donor EDTA plasma samples. RESULTS:Low HIV-1 RNA copy numbers (755 and 890 copies/0.1-mL input) that were detectable in two individual plasma specimens before HIV-1 p24 antigen were subsequently undetectable by GAT upon dilution with an equal volume of nonreactive plasma from a single donor. HIV-1 RNA at higher copy numbers (15,500 copies/0.1-mL input) in an HIV-1 p24 antigen-reactive and anti-HIV-1/2-nonreactive specimen was undetectable when diluted to 1-in-50 (1-in-50). Terminal dilution of seven HIV-1 RNA-containing plasma panel specimens indicated a proportional loss of HIV-1 RNA detectability with increasing dilution. CONCLUSION:GAT for detection of HIV-1 RNA in individual specimens was more sensitive than other HIV markers. For pooled plasma testing, GAT-independent constraints, such as effects of dilution, may preclude the use of GAT detection as a replacement for individual unit testing with HIV serologic assays.






Kemper M,Witt D,Madsen T,Kuramoto K,Holland P




Has Abstract


1998-05-01 00:00:00












  • Potential risk of blood transfusion-transmitted brucellosis in an endemic area of China.

    abstract:BACKGROUND:Brucellosis is a severe zoonotic disease that is increasingly prevalent in north China. A study evaluating Brucella infection in blood donors was conducted at Kashi central blood station, Xinjiang, China. STUDY DESIGN AND METHODS:Four serologic and two molecular methods of detection of Brucella infection we...


    pub_type: 杂志文章


    authors: Wang W,Liao Q,Wu X,Hou S,Wang Y,Wu J,Shen C,Chen S,Allain JP,Li C

    更新日期:2015-03-01 00:00:00

  • Effect of blood donation on the establishment of normal ranges of lymphocyte subsets.

    abstract:BACKGROUND:Absolute counts of CD4+ T-lymphocytes are used in the management of patients with human immunodeficiency virus infection. Low absolute counts of CD3+CD4+ cells have also been observed in healthy people--a phenomenon called idiopathic CD4 lymphocytopenia. It is common practice for normal ranges for lymphocyte...


    pub_type: 杂志文章


    authors: Bryant JA,Wylie BR,Yuan FF,Ribeiro A,Thomson AR,Cooley MA,Fletcher A

    更新日期:1996-06-01 00:00:00

  • A novel bioreactor and culture method drives high yields of platelets from stem cells.

    abstract:BACKGROUND:Platelet (PLT) transfusion is the primary treatment for thrombocytopenia. PLTs are obtained exclusively from volunteer donors, and the PLT product has only a 5-day shelf life, which can limit supply and result in PLT shortages. PLTs derived from stem cells could help to fill this clinical need. However, curr...


    pub_type: 杂志文章


    authors: Avanzi MP,Oluwadara OE,Cushing MM,Mitchell ML,Fischer S,Mitchell WB

    更新日期:2016-01-01 00:00:00

  • The risk of transfusion-transmitted infections in sub-Saharan Africa.

    abstract:BACKGROUND:Blood transfusions carry the risk of transmitting infections. This risk has been studied in detail in high-income countries but not in sub-Saharan Africa. This study estimates the risks of acquiring human immunodeficiency virus (HIV), hepatitis B virus (HBV), or hepatitis C virus (HCV) from a single unit of ...


    pub_type: 杂志文章,评审


    authors: Jayaraman S,Chalabi Z,Perel P,Guerriero C,Roberts I

    更新日期:2010-02-01 00:00:00

  • First Zika-positive donations in the continental United States.

    abstract:BACKGROUND:Zika virus (ZIKV) has spread in the Americas, including parts of the southern United States, and infection can be associated with serious complications, including congenital brain abnormalities. Probable transfusion transmission of ZIKV has been documented in Brazil. STUDY DESIGN AND METHODS:Preemptive test...


    pub_type: 杂志文章


    authors: Galel SA,Williamson PC,Busch MP,Stanek D,Bakkour S,Stone M,Lu K,Jones S,Rossmann SN,Pate LL,cobas Zika IND Study Group.

    更新日期:2017-03-01 00:00:00

  • Electronic enhancements to blood ordering reduce component waste.

    abstract:BACKGROUND:The effect on component wastage after enhancing the clinician's ability to order blood products in the computerized physician order entry (CPOE) system was investigated in a multihospital network. METHODS:A novel field was added to the CPOE of eight hospitals within a health care system allowing the physici...


    pub_type: 杂志文章


    authors: Yazer MH,Deandrade DS,Triulzi DJ,Wisniewski MK,Waters JH

    更新日期:2016-03-01 00:00:00

  • In vivo and in vitro characteristics of double units of RBCs collected by apheresis with a single in-line WBC-reduction filter.

    abstract:BACKGROUND:A novel apheresis procedure for a blood separator (MCS+, Haemonetics) enables the collection of 2 WBC-reduced RBC units in a single donation by using one disposable set with one in-line WBC-reduction filter (RC2H, Pall Corp.). The objective of this study was to evaluate the filtration performance in connecti...


    pub_type: 杂志文章


    authors: Bandarenko N,Rose M,Kowalsky RJ,Baston RK,Brecher ME,Elfath MD,Whitley P,Heminway M,Holme S

    更新日期:2001-11-01 00:00:00

  • Is red blood cell rheology preserved during routine blood bank storage?

    abstract:BACKGROUND:Red blood cell (RBC) units stored for more than 2 weeks at 4 degrees C are currently considered of impaired quality. This opinion has primarily been based on altered RBC rheologic properties (i.e., enhanced aggregability, reduced deformability, and elevated endothelial cell interaction), during prolonged sto...


    pub_type: 杂志文章


    authors: Henkelman S,Dijkstra-Tiekstra MJ,de Wildt-Eggen J,Graaff R,Rakhorst G,van Oeveren W

    更新日期:2010-04-01 00:00:00

  • Primary structure of human neutrophil antigens 1a and 1b.

    abstract:BACKGROUND:Neutrophil specific Fcγ receptor IIIb (CD16b) is a low-affinity IgG receptor. Its polymorphic variants are associated with human neutrophil antigens (HNA). HNA-1a and HNA-1b differ in four amino acids. Immunization can lead to the production of alloantibodies. The exact contribution of four amino acid exchan...


    pub_type: 杂志文章


    authors: Sachs UJ,Radke C,Bein G,Grabowski C,Simtong P,Bux J,Bayat B,Reil A

    更新日期:2020-04-01 00:00:00

  • The effects of cryopreservation on red blood cell rheologic properties.

    abstract:BACKGROUND:In transfusion medicine, frozen red blood cells (RBCs) are an alternative for liquid-stored RBCs. Little is known about the rheologic properties (i.e., aggregability and deformability) of thawed RBCs. In this study the rheologic properties of high-glycerol frozen RBCs and postthaw stored in saline-adenine-gl...


    pub_type: 杂志文章


    authors: Henkelman S,Lagerberg JW,Graaff R,Rakhorst G,Van Oeveren W

    更新日期:2010-11-01 00:00:00

  • Fatal erythroderma (suspected graft-versus-host disease) after cholecystectomy. Retrospective analysis.

    abstract::Fatal postoperative erythroderma (POE) developed in a 52-year-old woman with gallstones who underwent elective cholecystectomy. During surgery, she was transfused with 3 units of unirradiated packed red cells stored in the liquid state for at least 4 days after collection. The POE is believed to have been the result o...


    pub_type: 杂志文章


    authors: Otsuka S,Kunieda K,Hirose M,Takeuchi H,Mizutani Y,Nagaya M,Sato G,Kasuya S,Matsutomo K,Noma A

    更新日期:1989-07-01 00:00:00

  • Long-term return and donation pattern of those who begin donating at different ages: A retrospective cohort analysis of blood donors in Australia.

    abstract:BACKGROUND:This study compared the likelihood of return to donate and donation rate ratio by age of donors at their first donation when followed up to 12 years. STUDY DESIGN AND METHODS:Donation history of two cohorts of first-time donors (those donating in 2007 and 2013) was extracted until March 2019 from Australian...


    pub_type: 杂志文章


    authors: Priyono A,Masser BM,Dyda A,Davison TE,Irving DO,Karki S

    更新日期:2020-11-12 00:00:00

  • The effect of storage on degranulation by human neutrophils.

    abstract::We investigated the possibility that the functional impairment in neutrophil (PMN) chemotaxis which occurs during granulocyte concentrate storage might be due to autotoxicity from the release of neutrophil granule contents during storage. Preliminary experiments confirmed that the exposure of fresh PMNs to the intrace...


    pub_type: 杂志文章


    authors: Lane TA,Lamkin GE

    更新日期:1985-03-01 00:00:00

  • Clinical investigation of posttransfusion Kidd blood group typing using a rapid normalized quantitative polymerase chain reaction.

    abstract:BACKGROUND:Accurate typing of a patient's RBCs in the setting of prior transfusion or a hemolytic transfusion reaction is crucial in the selection of compatible blood but is time consuming, technically difficult, and sometimes impossible. To address this problem, a simple, rapid, and inexpensive quantitative PCR method...


    pub_type: 杂志文章


    authors: Montalvo L,Walker P,Wen L,Lim W,Reed W,Busch MP,Lee TH

    更新日期:2004-05-01 00:00:00

  • Evaluation of a new citrate-acetate-NaCl platelet additive solution for the storage of white cell-reduced platelet concentrates obtained from half-strength CPD pooled buffy coats.

    abstract:BACKGROUND:A new citrate-acetate-NaCl platelet additive solution, identified as PAS 2, was developed to prepare platelet concentrates (PCs) from pooled 0.5 CPD buffy coats (BCs). STUDY DESIGN AND METHODS:A study was undertaken to evaluate PAS 2 in vitro (n = 8) and in vivo (n = 9) against a commercially available solu...


    pub_type: 杂志文章


    authors: van Rhenen DJ,Vermeij H,Kappers-Klunne M,Payrat JM

    更新日期:1995-01-01 00:00:00

  • Evaluation of commercial antiglobulin sera over a two-year period. Part I. Anti-beta 1A, anti-alpha 2D, and anti-beta 1E levels.

    abstract::Antiglobulin sera from nine different manufacturers have been tested, over a two-year period, for their ability to detect the complement components beta 1A, alpha 2D, and beta 1E. The results demontrate considerable variation in the abilities of sera from different manufacturers to detect these components and indicate...


    pub_type: 杂志文章


    authors: Issitt PD,Issitt CH,Wilkinson SL

    更新日期:1974-03-01 00:00:00

  • Pictorial representation of transfusion over the years.

    abstract:BACKGROUND:The writings of the 17th and 19th centuries about experiments and debates about transfusion were often analyzed and discussed in articles and books, but an analysis of illustrations of transfusion during this pioneering epoch can throw new light on the subject. The first transfusion attempts were as sensatio...


    pub_type: 历史文章,杂志文章


    authors: Lefrère JJ,Danic B

    更新日期:2009-05-01 00:00:00

  • Delayed hemolytic transfusion reaction caused by anti-P1 antibody.

    abstract::A 67-year-old white woman received transfusions of a total of 87 units of whole blood and red blood cells during and within 48 hours following a pneumonectomy. Although she had previously received blood transfusions, unexpected antibodies were not detectable by routine screening. On the second postoperative day, she d...


    pub_type: 杂志文章


    authors: Chandeysson PL,Flye MW,Simpkins SM,Holland PV

    更新日期:1981-01-01 00:00:00

  • Preservation of red cell properties after virucidal phototreatment with dimethylmethylene blue.

    abstract:BACKGROUND:All published reports have described methods for virus photoinactivation which significantly alter red cell (RBC) properties during storage. In order to improve virucidal activity and reduce damage to RBCs, a series of phenothiazine derivatives were either synthesized or purified and screened for bacteriopha...


    pub_type: 杂志文章


    authors: Wagner SJ,Skripchenko A,Robinette D,Mallory DA,Cincotta L

    更新日期:1998-08-01 00:00:00

  • Anti-D alloimmunization after D-mismatched allogeneic hematopoietic stem cell transplantation in patients with hematologic diseases.

    abstract:BACKGROUND:The de novo development of anti-D after D-mismatched allogeneic hematopoietic stem cell transplantation (AHSCT) is a possibility that must be considered. The transfusion of D- blood components after AHSCT has been recommended but anti-D alloimmunization in this setting has been studied little. Thus, the aim ...


    pub_type: 杂志文章


    authors: Cid J,Lozano M,Fernández-Avilés F,Carreras E,Pereira A,Mazzara R,Ordinas A

    更新日期:2006-02-01 00:00:00

  • Platelet transfusion: automated selection of donors.

    abstract::The selection of platelet donors, according to HLA antigens, has been computerized using a method based on the calculation of incompatibilities and risk of immunization between donors and recipients. Since the risk that an antigen present in a donor will immunize an incompatible recipient of known HLA phenotype, an in...


    pub_type: 杂志文章


    authors: Muller JY,Cartron JP,Trillot P,Halle L,Roquin H

    更新日期:1981-05-01 00:00:00

  • Blood use in the ambulatory setting among elderly in the United States.

    abstract:BACKGROUND:Our study characterizes blood use in the ambulatory setting by US elderly Medicare beneficiaries during 2001. As the US population ages and delivery of health care in outpatient settings is on the rise, ambulatory blood utilization is expected to increase. There is currently a lack of broad population-based ...


    pub_type: 杂志文章


    authors: Menis M,Burwen DR,Holness L,Anderson SA

    更新日期:2009-06-01 00:00:00

  • In vivo viability and functional integrity of filtered platelets.

    abstract::The in vivo viability and functional integrity of filtered platelets were compared with those of nonfiltered platelets in a controlled study. On two occasions, after template bleeding time, 14 healthy volunteers underwent plateletpheresis and received 600 mg of aspirin. Autologous 111In-labeled platelets were transfus...


    pub_type: 杂志文章


    authors: Brecher ME,Pineda AA,Zylstra-Halling VW,Chowdhury S,Forstrom LA

    更新日期:1990-10-01 00:00:00

  • Quality of freeze-dried (lyophilized) quarantined single-donor plasma.

    abstract:BACKGROUND:Transfusion of plasma is a basic treatment for complex coagulopathies as well as in major blood loss. Early transfusion of plasma after trauma with major hemorrhage has been recommended by retrospective studies. However, the use of plasma is often hampered by the need to maintain a cold chain and the time ne...


    pub_type: 杂志文章


    authors: Bux J,Dickhörner D,Scheel E

    更新日期:2013-12-01 00:00:00

  • Incidence of Wra antigen and anti-Wra in a Spanish population.

    abstract:BACKGROUND:This study reports the incidence of Wr(a) antigen and anti-Wr(a) in Valencia, Spain. STUDY DESIGN AND METHODS:The incidence of the Wr(a) antigen in 110,000 healthy blood donors was estimated. Likewise, the incidence of anti-Wr(a) was analyzed in a population consisting of 730 healthy blood donors, 356 pregn...


    pub_type: 杂志文章


    authors: Arriaga F,Llopis F,de la Rubia J,Carpio N,Moscardó J,Marty ML

    更新日期:2005-08-01 00:00:00

  • Reduced alloimmunization in mice following repeated transfusion with pathogen-reduced platelets.

    abstract:BACKGROUND:Allogeneic transfusion can result in alloimmunization, leading to platelet (PLT) refractoriness and rejection of solid organ transplants. Previously we demonstrated that pathogen reduction using UV light and riboflavin (UV + R) eliminates the immunogenicity of white blood cells (WBCs) in vitro, blocks alloim...


    pub_type: 杂志文章


    authors: Muench MO,Heitman JW,Inglis H,Fomin ME,Marschner S,Goodrich RP,Norris PJ,Jackman RP

    更新日期:2016-06-01 00:00:00

  • Analysis of platelet-derived extracellular vesicles in plateletpheresis concentrates: a multicenter study.

    abstract:BACKGROUND:Routine quantification of platelet-derived extracellular vesicles (PL-EVs) may be useful in the quality control (QC) of platelet concentrates (PCs). The aim of this multicenter study was to establish and validate a consensus protocol for the standardized PL-EV quantification using conventional flow cytometer...


    pub_type: 杂志文章,多中心研究


    authors: Black A,Orsó E,Kelsch R,Pereira M,Kamhieh-Milz J,Salama A,Fischer MB,Meyer E,Frey BM,Schmitz G

    更新日期:2017-06-01 00:00:00

  • Predicting hemolytic disease of the newborn: a comparison of the monocyte monolayer assay and the chemiluminescence test.

    abstract::The ability of the monocyte monolayer assay (MMA) and the chemiluminescence test (CLT) to predict the clinical significance of alloantibodies associated with hemolytic disease of the newborn (HDN) was assessed by the use of 22 well-characterized antisera--predominantly anti-D--from alloimmunized pregnant women. Sevent...


    pub_type: 杂志文章


    authors: Lucas GF,Hadley AG,Nance SJ,Garratty G

    更新日期:1993-06-01 00:00:00

  • Acute lung injury after blood transfusion in mechanically ventilated patients.

    abstract:BACKGROUND:Liberal transfusion strategy increases the risk of acute lung injury (ALI), but specific transfusion-related factors have not been characterized. We tested the hypotheses that storage age and specific type of blood products are associated with increased risk of ALI in mechanically ventilated patients. STUDY...


    pub_type: 杂志文章


    authors: Gajic O,Rana R,Mendez JL,Rickman OB,Lymp JF,Hubmayr RD,Moore SB

    更新日期:2004-10-01 00:00:00

  • How to verify patient identity and blood product compatibility using an electronic bedside transfusion system.

    abstract::Transfusion of an incorrect blood component is an important avoidable serious hazard of transfusion resulting from process errors. Our group and others have taken advantage of new technology and developed electronic transfusion systems for safe transfusion practice in a previous studies. They allow the clinical staff ...


    pub_type: 杂志文章


    authors: Staples S,O'Callaghan C,Pavord S,Staves J,Murphy MF

    更新日期:2020-09-01 00:00:00