Abstract:
BACKGROUND:Recently, a more accurate and reliable screening test has been investigated for noninvasive prenatal fetal RHD genotyping from D- women. The objective of this study was to perform the new method of noninvasive fetal RHD genotyping with maternal plasma from D- women by use of capillary electrophoresis. STUDY DESIGN AND METHODS:Blood samples were obtained from 8 D+ and 8 D- nonpregnant donors and mixed to make test plasma samples. DNA was extracted and the appropriate conditions relating to the initial sample volume as well as polymerase chain reaction cycle numbers were analyzed to detect the RHD gene with RHD exon 10 primer. Blood samples were also obtained from 13 D- pregnant women ranging from the 12th to 39th weeks of gestation. The presence of the RHD gene and Y-chromosome-specific STR (Y-STR) derived from the fetus was analyzed. The results were compared with the D status of newborns. RESULTS:In samples from 12 D- pregnant women, the RHD gene was detected. In one sample, the RHD gene was not detected but Y-STR loci were demonstrated in this sample, indicating a D- male baby. The results of fetal genotyping were all in concordance with the postpartum samples by serologic tests on D as well as with the sex of newborns. CONCLUSION:Capillary electrophoresis can be used for the determination of fetal RHD status in D- women. This diagnostic method is useful for the noninvasive prenatal diagnosis of the fetal RHD genotyping from D- women.
journal_name
Transfusionjournal_title
Transfusionauthors
Kimura M,Sato C,Hara M,Ishihara O,Ikebuchi Kdoi
10.1111/j.1537-2995.2008.01681.xsubject
Has Abstractpub_date
2008-06-01 00:00:00pages
1156-63issue
6eissn
0041-1132issn
1537-2995pii
TRF01681journal_volume
48pub_type
杂志文章相关文献
TRANSFUSION文献大全abstract:BACKGROUND:With changing demographics of the United States population and the continuous need to recruit new donors, it is important to monitor the demographic profile of first-time donors and to evaluate changes in the donor pool to improve recruitment targeting. STUDY DESIGN AND METHODS:First-time whole blood (n = 9...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.2001.41030360.x
更新日期:2001-03-01 00:00:00
abstract:BACKGROUND:Bleeding into the chest is a life-threatening complication of cardiac surgery. Blood transfusion has been implicated as an important cause of harm associated with bleeding, based largely on studies demonstrating an independent association between transfusion and mortality. These studies did not, however, con...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2012.03697.x
更新日期:2013-01-01 00:00:00
abstract::Social and economic development, along with increased health care coverage, has caused a sharp increase in the clinical demand for blood in China. Whole blood collection has increased rapidly in the past decade but has failed to keep pace with the ever-increasing demand. Overall, the country's blood safety has been im...
journal_title:Transfusion
pub_type: 杂志文章,评审
doi:10.1111/trf.12273
更新日期:2014-02-01 00:00:00
abstract:BACKGROUND:The writings of the 17th and 19th centuries about experiments and debates about transfusion were often analyzed and discussed in articles and books, but an analysis of illustrations of transfusion during this pioneering epoch can throw new light on the subject. The first transfusion attempts were as sensatio...
journal_title:Transfusion
pub_type: 历史文章,杂志文章
doi:10.1111/j.1537-2995.2008.02068.x
更新日期:2009-05-01 00:00:00
abstract:BACKGROUND:The performance of the recently launched Procleix Ultrio (Chiron/Gen-Probe) human immunodeficiency virus-1 (HIV-1), hepatitis C virus (HCV), and hepatitis B virus (HBV) blood screening assay was evaluated in a European multicenter study. STUDY DESIGN AND METHODS:Serial dilutions of reference materials were ...
journal_title:Transfusion
pub_type: 杂志文章,多中心研究
doi:10.1111/j.1537-2995.2005.00197.x
更新日期:2005-08-01 00:00:00
abstract::A 27-year-old, gravida 3, para 2 woman experienced a delayed hemolytic transfusion reaction. She had anti-Dob in both serum and eluate 8 days after infusion of 6 units of Do(b+) red cells. No antibody had been detected prior to transfusion. By the 15th day after transfusion, there was no evidence of survival of red ce...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1985.25185116501.x
更新日期:1985-01-01 00:00:00
abstract:BACKGROUND:New alloantibody formation is unpredictable in patients who have been previously alloimmunized. Pretransfusion testing is designed to detect these antibodies while antibody identification (ABI) techniques are designed to identify the specificity of the antibody. Pretransfusion testing intervals are prescribe...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.13380
更新日期:2016-02-01 00:00:00
abstract::Blood from humans collected into heparin or citrate was inoculated with toxoplasma organisms. After storage at 4 C up to 28 days, samples were injected into the ear veins of rabbits. The test rabbits developed toxoplasmosis. Similar results were obtained by transfusing rabbits with blood obtained from rabbits subcutan...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1978.18378205142.x
更新日期:1978-05-01 00:00:00
abstract:BACKGROUND:Historically, paid blood donors were found to transmit hepatitis at higher rates than volunteers. In those older studies, paid donors frequently were recruited from prisons or slum areas--a finding consistent with the belief that monetary payment in itself did not necessarily lead to the high-risk status of ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1994.34294143937.x
更新日期:1994-02-01 00:00:00
abstract:BACKGROUND:All published reports have described methods for virus photoinactivation which significantly alter red cell (RBC) properties during storage. In order to improve virucidal activity and reduce damage to RBCs, a series of phenothiazine derivatives were either synthesized or purified and screened for bacteriopha...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1998.38898375511.x
更新日期:1998-08-01 00:00:00
abstract:BACKGROUND:Methods that detect a child's homozygosity by examination of allelic products are insensitive for diagnosing incest because, at a given locus, a homozygous state is expected with a frequency of only 0.25 when parents are first-degree relatives. Furthermore, these methods are not specific if the population co...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1994.34294143949.x
更新日期:1994-02-01 00:00:00
abstract:BACKGROUND:Brucellosis is a severe zoonotic disease that is increasingly prevalent in north China. A study evaluating Brucella infection in blood donors was conducted at Kashi central blood station, Xinjiang, China. STUDY DESIGN AND METHODS:Four serologic and two molecular methods of detection of Brucella infection we...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.12853
更新日期:2015-03-01 00:00:00
abstract:BACKGROUND:Autologous umbilical cord blood (AutoUCB) has historically been cryopreserved for potential use in hematopoietic transplantation. Increasingly, private AutoUCB banking is performed for therapies unavailable today. A Phase I trial using AutoUCB treatment for early pediatric Type 1 diabetes afforded us an oppo...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2011.03556.x
更新日期:2012-10-01 00:00:00
abstract:BACKGROUND:To avoid the need, in serial apheresis donors, either to delay plateletpheresis until a predonation platelet count is completed or to obtain a postdonation count after each procedure, a statistical model has been developed to predict the postdonation platelet count from the donor predonation platelet count, ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1994.34194098605.x
更新日期:1994-01-01 00:00:00
abstract::Originally pasted on walls and on locations reserved specially for that purpose, the poster is a medium for advertising and promotion to be seen on the streets and in public places. More recently, it has spread, in a smaller format, on dedicated indoor sites: billboards, columns, street furniture, and so forth. For tr...
journal_title:Transfusion
pub_type: 杂志文章,评审
doi:10.1111/j.1537-2995.2011.03422.x
更新日期:2012-06-01 00:00:00
abstract::As the number of successful marrow transplants has increased, the lack of HLA-identical sibling donors for 60 to 70 percent of transplant candidates has become a serious problem. Pilot studies established that marrow transplantation between phenotypically HLA-identical, unrelated individuals can be accomplished succes...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1993.33793325052.x
更新日期:1993-07-01 00:00:00
abstract:BACKGROUND:Reported hepatitis E virus (HEV) antibody assay performance characteristics are variable. Using a subset of surplus US blood donation samples, we compared assays for detecting anti-HEV immunoglobulin M (Ig)M and IgG or total anti-HEV antibodies. STUDY DESIGN AND METHODS:Samples from 5040 random blood donati...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.14553
更新日期:2018-05-01 00:00:00
abstract::Th activation of red cells is characterized by agglutination with the peanut lectin from Arachis hypogaea and is diminished by treatment with proteolytic enzymes. The first cases of Th activation were associated with bacterial infections. More recently, a high incidence of Th activation in congenital hypoplastic anemi...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1989.29789369684.x
更新日期:1989-09-01 00:00:00
abstract:BACKGROUND:According to Landsteiner's law, alloantibodies are prevalent and autoantibodies are absent in the ABO blood group system. However, one study (Spalter et al., Blood 1999;93:4418-24) has suggested that low-affinity ABO autoantibodies, mitigated by anti-idiotypic immunoglobulins are also prevalent, while anothe...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2011.03381.x
更新日期:2012-04-01 00:00:00
abstract:BACKGROUND:National guidance recommends planning for future blood shortages, but few studies have evaluated how reduced demand could be achieved acutely. STUDY DESIGN AND METHODS:A trained observer collected data concerning red blood cell (RBC) transfusion events outside the operating theater during 68 hours of blood ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2009.02244.x
更新日期:2009-10-01 00:00:00
abstract:BACKGROUND:Selection of CD34+ PBPCs has been applied as a method of reducing graft contamination from neoplastic cells. This procedure seems to delay lymphocyte recovery, while myeloid engraftment is no different from that with unselected PBPC transplants. STUDY DESIGN AND METHODS:Lymphocyte recovery was studied in tw...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.2001.41060783.x
更新日期:2001-06-01 00:00:00
abstract:BACKGROUND:Transfusion-related acute lung injury (TRALI) is one of the most serious adverse events following transfusion, and there is no specific treatment in clinical practice. However, regulatory T cells (Tregs) have been suggested to play a potential role in the treatment of TRALI. This study investigated whether i...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.15064
更新日期:2019-02-01 00:00:00
abstract:BACKGROUND:Adequate administration of Rh immune globulin requires an accurate determination of the number of D-positive cells in the circulation of D-negative women. Although several tests have been described for the detection of fetomaternal hemorrhage, there is still a need for a rapid, simple, and clinically relevan...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1998.38298193101.x
更新日期:1998-02-01 00:00:00
abstract:BACKGROUND:Blood has been imbued with powerful connotations through history and across cultures. Currently bestowed with scientific meaning, blood nevertheless carries symbolic resonance. This study examines these representations among practitioners and sheds light on the clinical and nonclinical factors that guide blo...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.13352
更新日期:2016-01-01 00:00:00
abstract:BACKGROUND:Cryobiologic variables responsible for cell injuries and freezing techniques applicable in medical cryopractice should be revised and/or reengineered for minimizing cryoinjuries and maximizing cell recovery. In this study, the efficacy of different cryopreservation protocols based on platelet (PLT) recovery ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2006.00706.x
更新日期:2006-02-01 00:00:00
abstract::The growth of two strains of Serratia marcescens in blood components was tested in this study. One of the strains had been implicated in the epidemic of transfusion-associated sepsis experienced in Denmark and Sweden in 1991. In whole blood with a final concentration of 100 colony-forming units per mL of S. marcescens...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1993.331094054615.x
更新日期:1993-10-01 00:00:00
abstract::A female patient developed serious hemolysis in association with the injection of a radiographic contrast medium (RCM). Serologic investigation of her serum suggested complement-mediated hemolysis, induced by an RCM-dependent IgM antibody in her serum. The antibody was of high titer and low avidity. The antibody showe...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1991.31992094673.x
更新日期:1991-11-01 00:00:00
abstract:BACKGROUND:Umbilical cord blood (UCB) can be used as hematopoietic stem cell source for transplantation. The success of a transplantation is highly correlated with the number of total nucleated cells (TNCs) and CD34+ cells in the UCB. Certain obstetric factors increase the yield of stem cells in the UCB. It is necessar...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2007.01617.x
更新日期:2008-05-01 00:00:00
abstract:BACKGROUND:The severe acute respiratory syndrome (SARS) was first described in February 2003. Close contact with symptomatic patients appears to be the main route of transmission, whereas blood transfusion transmission could not be ruled out. STUDY DESIGN AND METHODS:A SARS coronavirus (SARS-CoV) detection kit develop...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2004.03269.x
更新日期:2004-04-01 00:00:00
abstract::The ability to store platelets beyond 24 hours requires a functionally closed system. This study tested the ability of a cell separator bowl seal system to resist penetration of microbial contamination under normal running conditions and under extreme environmental stress. Three test organisms, Micrococcus luteus, Ser...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1990.30290162901.x
更新日期:1990-02-01 00:00:00