Noninvasive fetal RHD genotyping by maternal plasma with capillary electrophoresis.

Abstract:

BACKGROUND:Recently, a more accurate and reliable screening test has been investigated for noninvasive prenatal fetal RHD genotyping from D- women. The objective of this study was to perform the new method of noninvasive fetal RHD genotyping with maternal plasma from D- women by use of capillary electrophoresis. STUDY DESIGN AND METHODS:Blood samples were obtained from 8 D+ and 8 D- nonpregnant donors and mixed to make test plasma samples. DNA was extracted and the appropriate conditions relating to the initial sample volume as well as polymerase chain reaction cycle numbers were analyzed to detect the RHD gene with RHD exon 10 primer. Blood samples were also obtained from 13 D- pregnant women ranging from the 12th to 39th weeks of gestation. The presence of the RHD gene and Y-chromosome-specific STR (Y-STR) derived from the fetus was analyzed. The results were compared with the D status of newborns. RESULTS:In samples from 12 D- pregnant women, the RHD gene was detected. In one sample, the RHD gene was not detected but Y-STR loci were demonstrated in this sample, indicating a D- male baby. The results of fetal genotyping were all in concordance with the postpartum samples by serologic tests on D as well as with the sex of newborns. CONCLUSION:Capillary electrophoresis can be used for the determination of fetal RHD status in D- women. This diagnostic method is useful for the noninvasive prenatal diagnosis of the fetal RHD genotyping from D- women.

journal_name

Transfusion

journal_title

Transfusion

authors

Kimura M,Sato C,Hara M,Ishihara O,Ikebuchi K

doi

10.1111/j.1537-2995.2008.01681.x

subject

Has Abstract

pub_date

2008-06-01 00:00:00

pages

1156-63

issue

6

eissn

0041-1132

issn

1537-2995

pii

TRF01681

journal_volume

48

pub_type

杂志文章
  • First-time blood donors: demographic trends.

    abstract:BACKGROUND:With changing demographics of the United States population and the continuous need to recruit new donors, it is important to monitor the demographic profile of first-time donors and to evaluate changes in the donor pool to improve recruitment targeting. STUDY DESIGN AND METHODS:First-time whole blood (n = 9...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.2001.41030360.x

    authors: Wu Y,Glynn SA,Schreiber GB,Wright DJ,Lo A,Murphy EL,Kleinman SH,Garratty G,Retrovirus Epidemiology Donor Study (REDS) Group.

    更新日期:2001-03-01 00:00:00

  • The association of blood transfusion with mortality after cardiac surgery: cause or confounding? (CME).

    abstract:BACKGROUND:Bleeding into the chest is a life-threatening complication of cardiac surgery. Blood transfusion has been implicated as an important cause of harm associated with bleeding, based largely on studies demonstrating an independent association between transfusion and mortality. These studies did not, however, con...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2012.03697.x

    authors: Dixon B,Santamaria JD,Reid D,Collins M,Rechnitzer T,Newcomb AE,Nixon I,Yii M,Rosalion A,Campbell DJ

    更新日期:2013-01-01 00:00:00

  • Blood safety and availability: continuing challenges in China's blood banking system.

    abstract::Social and economic development, along with increased health care coverage, has caused a sharp increase in the clinical demand for blood in China. Whole blood collection has increased rapidly in the past decade but has failed to keep pace with the ever-increasing demand. Overall, the country's blood safety has been im...

    journal_title:Transfusion

    pub_type: 杂志文章,评审

    doi:10.1111/trf.12273

    authors: Shi L,Wang JX,Stevens L,Ness P,Shan H

    更新日期:2014-02-01 00:00:00

  • Pictorial representation of transfusion over the years.

    abstract:BACKGROUND:The writings of the 17th and 19th centuries about experiments and debates about transfusion were often analyzed and discussed in articles and books, but an analysis of illustrations of transfusion during this pioneering epoch can throw new light on the subject. The first transfusion attempts were as sensatio...

    journal_title:Transfusion

    pub_type: 历史文章,杂志文章

    doi:10.1111/j.1537-2995.2008.02068.x

    authors: Lefrère JJ,Danic B

    更新日期:2009-05-01 00:00:00

  • Multicenter performance evaluation of a transcription-mediated amplification assay for screening of human immunodeficiency virus-1 RNA, hepatitis C virus RNA, and hepatitis B virus DNA in blood donations.

    abstract:BACKGROUND:The performance of the recently launched Procleix Ultrio (Chiron/Gen-Probe) human immunodeficiency virus-1 (HIV-1), hepatitis C virus (HCV), and hepatitis B virus (HBV) blood screening assay was evaluated in a European multicenter study. STUDY DESIGN AND METHODS:Serial dilutions of reference materials were ...

    journal_title:Transfusion

    pub_type: 杂志文章,多中心研究

    doi:10.1111/j.1537-2995.2005.00197.x

    authors: Koppelman MH,Assal A,Chudy M,Torres P,de Villaescusa RG,Reesink HW,Lelie PN,Cuypers HT

    更新日期:2005-08-01 00:00:00

  • Anti-Dob implicated as the cause of a delayed hemolytic transfusion reaction.

    abstract::A 27-year-old, gravida 3, para 2 woman experienced a delayed hemolytic transfusion reaction. She had anti-Dob in both serum and eluate 8 days after infusion of 6 units of Do(b+) red cells. No antibody had been detected prior to transfusion. By the 15th day after transfusion, there was no evidence of survival of red ce...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1985.25185116501.x

    authors: Moheng MC,McCarthy P,Pierce SR

    更新日期:1985-01-01 00:00:00

  • Can the interval between antibody identifications be increased for alloimmunized patients?

    abstract:BACKGROUND:New alloantibody formation is unpredictable in patients who have been previously alloimmunized. Pretransfusion testing is designed to detect these antibodies while antibody identification (ABI) techniques are designed to identify the specificity of the antibody. Pretransfusion testing intervals are prescribe...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.13380

    authors: Goss C,Avecilla ST,Garbaini J,Degtyaryova D,Lo D,Chang DY,Cushing M

    更新日期:2016-02-01 00:00:00

  • Toxoplasmosis transmitted by blood transfusions.

    abstract::Blood from humans collected into heparin or citrate was inoculated with toxoplasma organisms. After storage at 4 C up to 28 days, samples were injected into the ear veins of rabbits. The test rabbits developed toxoplasmosis. Similar results were obtained by transfusing rabbits with blood obtained from rabbits subcutan...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1978.18378205142.x

    authors: Räisänen S

    更新日期:1978-05-01 00:00:00

  • Concurrent comparison of the safety of paid cytapheresis and volunteer whole-blood donors.

    abstract:BACKGROUND:Historically, paid blood donors were found to transmit hepatitis at higher rates than volunteers. In those older studies, paid donors frequently were recruited from prisons or slum areas--a finding consistent with the belief that monetary payment in itself did not necessarily lead to the high-risk status of ...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1994.34294143937.x

    authors: Strauss RG,Ludwig GA,Smith MV,Villhauer PJ,Randels MJ,Smith-Floss A,Koerner TA

    更新日期:1994-02-01 00:00:00

  • Preservation of red cell properties after virucidal phototreatment with dimethylmethylene blue.

    abstract:BACKGROUND:All published reports have described methods for virus photoinactivation which significantly alter red cell (RBC) properties during storage. In order to improve virucidal activity and reduce damage to RBCs, a series of phenothiazine derivatives were either synthesized or purified and screened for bacteriopha...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1998.38898375511.x

    authors: Wagner SJ,Skripchenko A,Robinette D,Mallory DA,Cincotta L

    更新日期:1998-08-01 00:00:00

  • Incest diagnosis by comparison of alleles of mother and offspring at highly heterozygous loci.

    abstract:BACKGROUND:Methods that detect a child's homozygosity by examination of allelic products are insensitive for diagnosing incest because, at a given locus, a homozygous state is expected with a frequency of only 0.25 when parents are first-degree relatives. Furthermore, these methods are not specific if the population co...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1994.34294143949.x

    authors: Wenk RE,Chiafari FA,Houtz T

    更新日期:1994-02-01 00:00:00

  • Potential risk of blood transfusion-transmitted brucellosis in an endemic area of China.

    abstract:BACKGROUND:Brucellosis is a severe zoonotic disease that is increasingly prevalent in north China. A study evaluating Brucella infection in blood donors was conducted at Kashi central blood station, Xinjiang, China. STUDY DESIGN AND METHODS:Four serologic and two molecular methods of detection of Brucella infection we...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.12853

    authors: Wang W,Liao Q,Wu X,Hou S,Wang Y,Wu J,Shen C,Chen S,Allain JP,Li C

    更新日期:2015-03-01 00:00:00

  • Characteristics of thawed autologous umbilical cord blood.

    abstract:BACKGROUND:Autologous umbilical cord blood (AutoUCB) has historically been cryopreserved for potential use in hematopoietic transplantation. Increasingly, private AutoUCB banking is performed for therapies unavailable today. A Phase I trial using AutoUCB treatment for early pediatric Type 1 diabetes afforded us an oppo...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2011.03556.x

    authors: Rosenau EH,Sugrue MW,Haller M,Fisk D,Kelly SS,Chang M,Hou W,Eldjerou L,Slayton W,Cogle CR,Wingard JR

    更新日期:2012-10-01 00:00:00

  • A statistical model for estimating donor postdonation platelet counts after plateletpheresis.

    abstract:BACKGROUND:To avoid the need, in serial apheresis donors, either to delay plateletpheresis until a predonation platelet count is completed or to obtain a postdonation count after each procedure, a statistical model has been developed to predict the postdonation platelet count from the donor predonation platelet count, ...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1994.34194098605.x

    authors: Cabezudo I,Winegarden DK,Randels MJ,Strauss RG

    更新日期:1994-01-01 00:00:00

  • Blood donation on posters: a worldwide review.

    abstract::Originally pasted on walls and on locations reserved specially for that purpose, the poster is a medium for advertising and promotion to be seen on the streets and in public places. More recently, it has spread, in a smaller format, on dedicated indoor sites: billboards, columns, street furniture, and so forth. For tr...

    journal_title:Transfusion

    pub_type: 杂志文章,评审

    doi:10.1111/j.1537-2995.2011.03422.x

    authors: Lefrère JJ,Danic B

    更新日期:2012-06-01 00:00:00

  • The National Marrow Donor Program.

    abstract::As the number of successful marrow transplants has increased, the lack of HLA-identical sibling donors for 60 to 70 percent of transplant candidates has become a serious problem. Pilot studies established that marrow transplantation between phenotypically HLA-identical, unrelated individuals can be accomplished succes...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1993.33793325052.x

    authors: Stroncek D,Bartsch G,Perkins HA,Randall BL,Hansen JA,McCullough J

    更新日期:1993-07-01 00:00:00

  • Disparities in detection of antibodies against hepatitis E virus in US blood donor samples using commercial assays.

    abstract:BACKGROUND:Reported hepatitis E virus (HEV) antibody assay performance characteristics are variable. Using a subset of surplus US blood donation samples, we compared assays for detecting anti-HEV immunoglobulin M (Ig)M and IgG or total anti-HEV antibodies. STUDY DESIGN AND METHODS:Samples from 5040 random blood donati...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.14553

    authors: Zafrullah M,Zhang X,Tran C,Nguyen M,Kamili S,Purdy MA,Stramer SL

    更新日期:2018-05-01 00:00:00

  • Th activation of maternal and cord blood.

    abstract::Th activation of red cells is characterized by agglutination with the peanut lectin from Arachis hypogaea and is diminished by treatment with proteolytic enzymes. The first cases of Th activation were associated with bacterial infections. More recently, a high incidence of Th activation in congenital hypoplastic anemi...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1989.29789369684.x

    authors: Wahl CM,Herman JH,Shirey RS,Kickler TS,Ness PM

    更新日期:1989-09-01 00:00:00

  • Natural anti-A and anti-B of the ABO system: allo- and autoantibodies have different epitope specificity.

    abstract:BACKGROUND:According to Landsteiner's law, alloantibodies are prevalent and autoantibodies are absent in the ABO blood group system. However, one study (Spalter et al., Blood 1999;93:4418-24) has suggested that low-affinity ABO autoantibodies, mitigated by anti-idiotypic immunoglobulins are also prevalent, while anothe...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2011.03381.x

    authors: Obukhova P,Korchagina E,Henry S,Bovin N

    更新日期:2012-04-01 00:00:00

  • Red blood cell use outside the operating theater: a prospective observational study with modeling of potential blood conservation during severe blood shortages.

    abstract:BACKGROUND:National guidance recommends planning for future blood shortages, but few studies have evaluated how reduced demand could be achieved acutely. STUDY DESIGN AND METHODS:A trained observer collected data concerning red blood cell (RBC) transfusion events outside the operating theater during 68 hours of blood ...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2009.02244.x

    authors: Joseph BG,Hendry C,Walsh TS

    更新日期:2009-10-01 00:00:00

  • Immune reconstitution after autologous selected peripheral blood progenitor cell transplantation: comparison of two CD34+ cell-selection systems.

    abstract:BACKGROUND:Selection of CD34+ PBPCs has been applied as a method of reducing graft contamination from neoplastic cells. This procedure seems to delay lymphocyte recovery, while myeloid engraftment is no different from that with unselected PBPC transplants. STUDY DESIGN AND METHODS:Lymphocyte recovery was studied in tw...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.2001.41060783.x

    authors: Laurenti L,Sorà F,Piccirillo N,Chiusolo P,Cicconi S,Rutella S,Serafini R,Garzia MG,Leone G,Sica S

    更新日期:2001-06-01 00:00:00

  • Preventing murine transfusion-related acute lung injury by expansion of CD4+ CD25+ FoxP3+ Tregs using IL-2/anti-IL-2 complexes.

    abstract:BACKGROUND:Transfusion-related acute lung injury (TRALI) is one of the most serious adverse events following transfusion, and there is no specific treatment in clinical practice. However, regulatory T cells (Tregs) have been suggested to play a potential role in the treatment of TRALI. This study investigated whether i...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.15064

    authors: He R,Li L,Kong Y,Tian L,Tian X,Fang P,Bian M,Liu Z

    更新日期:2019-02-01 00:00:00

  • Use of the gel agglutination technique for determination of fetomaternal hemorrhage.

    abstract:BACKGROUND:Adequate administration of Rh immune globulin requires an accurate determination of the number of D-positive cells in the circulation of D-negative women. Although several tests have been described for the detection of fetomaternal hemorrhage, there is still a need for a rapid, simple, and clinically relevan...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1998.38298193101.x

    authors: Salama A,David M,Wittmann G,Stelzer A,Dudenhausen JW

    更新日期:1998-02-01 00:00:00

  • Blood transfusion in acute and chronic pediatric settings: beliefs and practices.

    abstract:BACKGROUND:Blood has been imbued with powerful connotations through history and across cultures. Currently bestowed with scientific meaning, blood nevertheless carries symbolic resonance. This study examines these representations among practitioners and sheds light on the clinical and nonclinical factors that guide blo...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.13352

    authors: Fortin S,Cardona LG,Latreille M,Tucci M,Lacroix J

    更新日期:2016-01-01 00:00:00

  • Controlled-rate versus uncontrolled-rate freezing as predictors for platelet cryopreservation efficacy.

    abstract:BACKGROUND:Cryobiologic variables responsible for cell injuries and freezing techniques applicable in medical cryopractice should be revised and/or reengineered for minimizing cryoinjuries and maximizing cell recovery. In this study, the efficacy of different cryopreservation protocols based on platelet (PLT) recovery ...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2006.00706.x

    authors: Balint B,Paunovic D,Vucetic D,Vojvodic D,Petakov M,Trkuljic M,Stojanovic N

    更新日期:2006-02-01 00:00:00

  • Transfusion-associated Serratia marcescens infection: studies of the mechanism of action.

    abstract::The growth of two strains of Serratia marcescens in blood components was tested in this study. One of the strains had been implicated in the epidemic of transfusion-associated sepsis experienced in Denmark and Sweden in 1991. In whole blood with a final concentration of 100 colony-forming units per mL of S. marcescens...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1993.331094054615.x

    authors: Gong J,Högman CF,Hambraeus A,Johansson CS,Eriksson L

    更新日期:1993-10-01 00:00:00

  • Immune-mediated hemolysis associated with the administration of a radiographic contrast medium.

    abstract::A female patient developed serious hemolysis in association with the injection of a radiographic contrast medium (RCM). Serologic investigation of her serum suggested complement-mediated hemolysis, induced by an RCM-dependent IgM antibody in her serum. The antibody was of high titer and low avidity. The antibody showe...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1991.31992094673.x

    authors: Nordhagen R,Vik H,Wolthuis K,Bøhn HP,Urdahl P,Michaelsen TE

    更新日期:1991-11-01 00:00:00

  • Cesarean section due to fetal distress increases the number of stem cells in umbilical cord blood.

    abstract:BACKGROUND:Umbilical cord blood (UCB) can be used as hematopoietic stem cell source for transplantation. The success of a transplantation is highly correlated with the number of total nucleated cells (TNCs) and CD34+ cells in the UCB. Certain obstetric factors increase the yield of stem cells in the UCB. It is necessar...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2007.01617.x

    authors: Manegold G,Meyer-Monard S,Tichelli A,Pauli D,Holzgreve W,Troeger C

    更新日期:2008-05-01 00:00:00

  • NAT screening of blood donors for severe acute respiratory syndrome coronavirus can potentially prevent transfusion associated transmissions.

    abstract:BACKGROUND:The severe acute respiratory syndrome (SARS) was first described in February 2003. Close contact with symptomatic patients appears to be the main route of transmission, whereas blood transfusion transmission could not be ruled out. STUDY DESIGN AND METHODS:A SARS coronavirus (SARS-CoV) detection kit develop...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2004.03269.x

    authors: Schmidt M,Brixner V,Ruster B,Hourfar MK,Drosten C,Preiser W,Seifried E,Roth WK

    更新日期:2004-04-01 00:00:00

  • Microbial challenge of a blood cell separator outside-seal bowl system.

    abstract::The ability to store platelets beyond 24 hours requires a functionally closed system. This study tested the ability of a cell separator bowl seal system to resist penetration of microbial contamination under normal running conditions and under extreme environmental stress. Three test organisms, Micrococcus luteus, Ser...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1990.30290162901.x

    authors: Jacobson MS,Kevy SV,Thorne GM,Goldmann DA,Blasetti L,Smith JW,Schaefer H

    更新日期:1990-02-01 00:00:00