Abstract:
BACKGROUND:After multiple transfusions, the serologic typing of autologous blood group phenotypes is difficult, because of mixed RBC populations. The genotyping of ABO, Rh, Kell, Kidd, and Duffy systems could be used to determine autologous blood group antigen status. STUDY DESIGN AND METHODS:Blood samples from patients and donors were analyzed before and after 26 multiple-transfusion events. An average of 6.9 non-WBC-reduced RBC units with an average age of 5.9 days were administered per transfusion event. The average period of blood sampling after transfusions was 5.3 days. All samples were serologically phenotyped for ABO, Rh, Kell, Kidd, and Duffy. Pretransfusion, posttransfusion, and buccal samples from patients were genotyped for the corresponding alleles by a uniform PCR sequence-specific primer protocol that allowed their simultaneous determination within 3 hours. RESULTS:All posttransfusion samples exhibited mixed-cell populations of various blood group systems on serologic testing. Genotyping from peripheral blood produced results identical to the autologous blood group phenotypes, regardless of the amount of blood transfused or of the length of the sampling period after transfusion. CONCLUSION:A fast and reliable PCR-sequence-specific primer DNA genotyping assay for simultaneous determination of autologous ABO, Rh, Kell, Kidd, and Duffy blood groups can be performed on peripheral blood samples, even though the patients have recently received multiple transfusions.
journal_name
Transfusionjournal_title
Transfusionauthors
Rozman P,Dovc T,Gassner Cdoi
10.1046/j.1537-2995.2000.40080936.xsubject
Has Abstractpub_date
2000-08-01 00:00:00pages
936-42issue
8eissn
0041-1132issn
1537-2995journal_volume
40pub_type
杂志文章相关文献
TRANSFUSION文献大全abstract:BACKGROUND:Transfusion of the incorrect blood component is a frequent serious incident associated with transfusion and often involves misidentification of the patient and/or the unit of blood. The objective of this study was to assess the effect of a simple intervention designed to improve performance of the bedside ch...
journal_title:Transfusion
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1111/j.1537-2995.2007.01189.x
更新日期:2007-05-01 00:00:00
abstract:BACKGROUND:Throughout history, blood has been embedded with powerful and paradoxical connotations. In the last decades, a more rational conception based on scientific knowledge has emerged, portraying blood as a "disenchanted" and utilitarian object, hence more apt for donation between strangers. This study aims to exa...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.12477
更新日期:2013-12-01 00:00:00
abstract:BACKGROUND:In August 2016, the Food and Drug Administration advised US blood centers to screen all whole blood and apheresis donations for Zika virus (ZIKV) with an individual-donor nucleic acid test (ID-NAT) or to use approved pathogen reduction technology (PRT). The cost of implementing this guidance nationally has n...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.14164
更新日期:2017-06-01 00:00:00
abstract::The number of cases of transfusion-associated acquired immune deficiency syndrome (TA-AIDS) that will be seen over the next few years is difficult to estimate, because of the uncertainty about the number of persons infected with the human immunodeficiency virus (HIV) via blood transfusion and about the duration of the...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1989.29890020437.x
更新日期:1989-10-01 00:00:00
abstract:BACKGROUND:Five cases of human immunodeficiency virus Type 1 (HIV-1) RNA-positive blood donations are described that escaped detection by three different CE-marked nucleic acid amplification technique (NAT) screening assays. These events were associated with two HIV-1 transmissions to recipients of blood components. Th...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2011.03281.x
更新日期:2012-02-01 00:00:00
abstract::Current standards for the preparation of factor VIII (FVIII) concentrates from human plasma recommend separation of plasma from red cells (RBCs) within 6 hours of blood donation, thereby reducing the volume of plasma from donated whole blood available for processing to FVIII concentrate. The decay of FVIII clotting ac...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1988.28689059033.x
更新日期:1988-11-01 00:00:00
abstract:BACKGROUND:After allogeneic peripheral blood progenitor cell (PBPC) transplantation, a patient developed a severe hemolytic transfusion reaction due to passenger lymphocyte syndrome. CASE REPORT:A 50-year-old woman with secondary acute myeloid leukemia transforming from a myelodysplastic syndrome received an ABO-compa...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.2000.40060632.x
更新日期:2000-06-01 00:00:00
abstract:BACKGROUND:Maternal iron deficiency anemia (IDA) impacts placenta and fetus. We evaluated effects of IDA at admission for delivery on cesarean rates, and adverse maternal and neonatal outcomes. STUDY DESIGN AND METHODS:Medical records from Jerusalem (2005-2012) identified women with a live-birth singleton fetus in cep...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.13252
更新日期:2015-12-01 00:00:00
abstract:BACKGROUND:Umbilical cord blood (UCB) transplantation is becoming more widely used, yet ethical and policy issues regarding consent and health history persist. Whereas most UCB banks do not require paternal consent or paternal health history (PHH), both are obtained at this institution whenever possible. This study ass...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.2002.00188.x
更新日期:2002-10-01 00:00:00
abstract:BACKGROUND:Dendritic cells (DCs) are the most potent antigen-presenting cells that initiate and regulate immune responses. They are unique in their feature to produce bioactive interleukin (IL)-12, a major proinflammatory cytokine connecting innate and adaptive immunity. Platelets (PLTs) are highly reactive components ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2006.00802.x
更新日期:2006-05-01 00:00:00
abstract::Prior studies have suggested that a high percentage of blood transfusions to hospital patients are unnecessary. If such transfusions can be reduced, more blood would be made available for those patients with legitimate blood requirements. In this study, blood transfusion of 401 nonoperated hospital patients with anemi...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1978.18278160583.x
更新日期:1978-03-01 00:00:00
abstract:BACKGROUND:Umbilical cord blood (UCB) can be used as hematopoietic stem cell source for transplantation. The success of a transplantation is highly correlated with the number of total nucleated cells (TNCs) and CD34+ cells in the UCB. Certain obstetric factors increase the yield of stem cells in the UCB. It is necessar...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2007.01617.x
更新日期:2008-05-01 00:00:00
abstract:BACKGROUND:Autoimmune hemolytic anemia (AIHA) is usually classified as either warm or cold type. During the past few decades, mixed types (Mxs) have also been described in a number of cases (6%-8% of AIHA), often without serologic data to support the diagnosis. In this study, we demonstrate that the incidence of Mx AIH...
journal_title:Transfusion
pub_type: 杂志文章,评审
doi:10.1111/j.1537-2995.2008.01805.x
更新日期:2008-10-01 00:00:00
abstract:BACKGROUND:Clinical outcomes in transfused patients may be affected by the duration of blood storage, possibly due to red blood cell (RBC)-mediated disruption of nitric oxide (NO) signaling, a key regulator of vascular tone and blood flow. STUDY DESIGN AND METHODS:AS-1 RBC units stored up to 42 days were sampled at se...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.12111
更新日期:2013-11-01 00:00:00
abstract:BACKGROUND:Allogeneic blood cell transplantation is a new alternative to bone marrow transplantation. Preliminary data suggest that granulocyte-colony-stimulating factor (G-CSF)-mobilized peripheral blood progenitor cells from normal donors can provide rapid hematopoietic engraftment without significant increases in tr...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1996.36111297091734.x
更新日期:1996-11-01 00:00:00
abstract:BACKGROUND:Detection of viral nucleic acids might increase blood transfusion safety through the detection of recently infected blood donors during the preseroconversion window period. Individual screening is difficult to apply, because of technical and financial constraints. STUDY DESIGN AND METHODS:A polymerase chain...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1998.381098440854.x
更新日期:1998-10-01 00:00:00
abstract:BACKGROUND:Studies analyzing motivation factors that lead to blood donation have found altruism to be the primary motivation factor; however, social capital has not been analyzed in this context. Our study examines the association between motivation factors (altruism, self-interest, and response to direct appeal) and s...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2012.03887.x
更新日期:2013-06-01 00:00:00
abstract::Two patients with naturally occurring anti-K agglutinins are reported. Both patients manifested clinical symptoms associated with septicemia. Transfusion of one patient with K-positive blood did not result in hemolytic complications, further stimulation of the anti-K agglutinin or any change in its immunoglobulin natu...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1981.21281178154.x
更新日期:1981-03-01 00:00:00
abstract:BACKGROUND:Blood collection centers are charged with creating donor educational materials (DnEM) that are easily understood across all prospective donor populations, while addressing mandates and recommendations from regulatory agencies and professional standard setting organizations. Donors must have sufficient inform...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.15866
更新日期:2020-08-01 00:00:00
abstract:BACKGROUND:Transfusion medicine lacks a standard method for the systematic collection and analysis of event reports. Review of event reports from the Food and Drug Administration (FDA) showed a relative paucity of information on event causation. Thus, a causal analysis method was developed as part of a prototype Medica...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1998.38111299056319.x
更新日期:1998-11-01 00:00:00
abstract:BACKGROUND:Qualification of a cord blood bank (CBB) is a complex process that includes evaluation of multiple aspects of donor screening and testing, processing, accreditation and approval by professional cell therapy groups, and results of received cord blood units. The University of Minnesota Medical Center Cell Ther...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.12085
更新日期:2013-10-01 00:00:00
abstract:BACKGROUND:To prevent donor loss and improve retention, it is important to understand the major deterrents to blood donation and to identify factors that can be effectively addressed by blood centers. STUDY DESIGN AND METHODS:A 30-item self-administered questionnaire was completed in 2003 by 1705 first-time and 2437 r...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2006.00757.x
更新日期:2006-04-01 00:00:00
abstract::Blood centrifugation, with a continuous-flow or semicontinuous-flow system, was used in the treatment of 17 patients with various hematologic disorders. Total plasma exchange (TPE) controlled symptoms of hyperviscosity and arrested bleeding in three patients. In two patients, TPE made is possible to perform urgent sur...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1977.17578014576.x
更新日期:1977-09-01 00:00:00
abstract:BACKGROUND:Chronic red blood cell exchanges (RBCXs) are frequently used to prevent complications in patients with sickle cell anemia, but the scarcity of matched red blood cell packs (RBCPs) is a serious concern. The main goal of this study was to compare the number of RBCPs used during RBCXs between the Spectra Optia ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.15188
更新日期:2019-05-01 00:00:00
abstract:BACKGROUND:Human infections with simian foamy viruses (SFVs) have been reported after occupational and nonoccupational exposure to infected animals and their tissues, blood, and body fluids, although there is no evidence for human-to-human transmission. We previously demonstrated SFV transmission in monkeys by blood tr...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2009.02372.x
更新日期:2010-01-01 00:00:00
abstract::Fatal postoperative erythroderma (POE) developed in a 52-year-old woman with gallstones who underwent elective cholecystectomy. During surgery, she was transfused with 3 units of unirradiated packed red cells stored in the liquid state for at least 4 days after collection. The POE is believed to have been the result o...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1989.29689318456.x
更新日期:1989-07-01 00:00:00
abstract::Cell pheresis was employed in the initial management of a pregnant patient with chronic myelocytic leukemia (CML). Reduction in circulating leukocyte and platelet counts was evident after two weeks of therapy. This technique is an effective mode of management of the pregnant patient with CML and may play an important ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1978.18178118555.x
更新日期:1978-01-01 00:00:00
abstract:BACKGROUND:To estimate the impact of RBC preparations on the status of postoperative immune activation, the soluble cytokine receptors of TNFalpha (sTNF-R) and IL-2 (sIL-2R), as well as neopterin and cell-mediated lympholysis (CML), were measured. STUDY DESIGN AND METHODS:Patients undergoing strictly standardized anes...
journal_title:Transfusion
pub_type: 临床试验,杂志文章
doi:10.1046/j.1537-2995.2000.40070821.x
更新日期:2000-07-01 00:00:00
abstract:BACKGROUND:Borrelia miyamotoi, a recently discovered relapsing fever spirochete, occurs in hard-bodied ticks wherever Lyme disease is endemic. Human infection is associated with relapsing fever and can cause meningoencephalitis in immunocompromised patients. A few cases of transfusion transmission of other relapsing fe...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.12879
更新日期:2015-03-01 00:00:00
abstract::Anti-Hy, in a serum of a black man who had received multiple blood transfusions, was found to be a warm reactive and polyclonal IgG antibody which did not bind complement in vitro. 51Cr-labeled Hy-positive red blood cells in the patient showed a shortened "2-component" survival curve. Hy antigens were not detected on ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1975.15676082238.x
更新日期:1975-11-01 00:00:00