Abstract：
:In this study we describe for the first time the production of stable human B cell lines and clones that secrete IgM antibody specific for human myelin basic protein. The technique based on limiting dilutions of Epstein-Barr virus-transformed peripheral B cells from patients with multiple sclerosis, precluded the need for preselecting or stimulating antigen-specific B cells. Most of the cell lines were stable for at least 6 months in continuous culture and produced 5-12 micrograms/ml antibody after 2 weeks in culture. The myelin basic protein-specific B cells were surface IgM positive, and occurred with a frequency of approximately 1/2500 mononuclear cells in peripheral blood. The successful selection and quantitation of specific B cell clones described here suggests that this technique is well suited for evaluating B cell responses to known and suspected antigens and autoantigens.
journal_name
J Neuroimmunoljournal_title
Journal of neuroimmunologyauthors
Zhang JW,Lambrechts J,Heyligen H,Vandenbark AA,Raus JCdoi
10.1016/0165-5728(89)90092-1subject
Has Abstractpub_date
1989-09-01 00:00:00pages
9-16issue
1-2eissn
0165-5728issn
1872-8421pii
0165-5728(89)90092-1journal_volume
24pub_type
杂志文章abstract：:Our work consists of a pilot study to characterize circulating Th/c1, Th/c2, Th/c17, Treg and Tfh-like populations and IL-17 serum levels of relapsing-remitting (RR) MS patients treated with IFN-β, compared with healthy controls. In remission RRMS patients, we observe increased Th/c17 cells frequency committed to a Th...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/j.jneuroim.2019.577113
更新日期：2020-02-15 00:00:00
abstract：:Berberine is an herbal alkaloid with various biological activities, including anti-inflammatory and antidepressant effects. Here, we examined the effects of berberine on dopamine receptors and the ensuing anti-inflammatory responses. Berberine was found to be an antagonist at both dopamine D1- and D2-like receptors an...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/j.jneuroim.2015.10.001
更新日期：2015-12-15 00:00:00
abstract：:Multiple sclerosis (MS) is a chronic neuroinflammatory disease of the central nervous system that leads to demyelination and neurodegeneration. VIP and PACAP are structurally related neuropeptides with neuroprotective and anti-inflammatory activities. To evaluate VIP and PACAP-38 in plasma and CSF in humans in correla...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/j.jneuroim.2013.08.012
更新日期：2013-10-15 00:00:00
abstract：:Interferon-beta (IFN-beta) achieves its beneficial effect on multiple sclerosis (MS) via anti-inflammatory properties. In this study, we assessed the expression of the brain-derived neurotrophic factor (BDNF) in peripheral blood mononuclear cells (PBMC) from relapsing-remitting multiple sclerosis (RRMS) patients treat...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/j.jneuroim.2008.04.033
更新日期：2008-07-15 00:00:00
abstract：:The pathogenesis of Guillain-Barré Syndrome (GBS) is not entirely understood, but includes infection-induced aberrant immune responses. Genetic polymorphisms in Fc gamma receptor genes (FCGR) have been associated with GBS. We assessed whether polymorphisms rs1801274 in FCGR2A and rs396991 in FCGR3A were associated wit...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/j.jneuroim.2016.07.020
更新日期：2016-09-15 00:00:00
abstract：:We have established a mouse experimental brain abscess model using Staphylococcus aureus where lesion sites are greatly exaggerated compared to the localized area of initial infection, reminiscent of an overactive immune response. Here we demonstrate the prolonged expression of IL-1 beta, TNF-alpha, and macrophage inf...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/j.jneuroim.2004.02.002
更新日期：2004-06-01 00:00:00
abstract：:The skin response to myelin basic protein (MBP) was studied in chronic relapsing experimental allergic encephalomyelitis (EAE) using strain 13 guinea pigs. The delayed type skin response showed a monophasic curve; it gradually increased after immunization, reached maximum levels around 80 days post-immunization, and d...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/0165-5728(83)90050-4
更新日期：1983-12-01 00:00:00
abstract：:The blood brain barrier (BBB) and the blood-cerebrospinal fluid barrier (BCB) limit the influx of immune mediators and bloodstream compounds into the central nervous system (CNS). Upon injury or infection, the integrity of these barriers is compromised and leukocyte infiltration occurs. The BCB is located in the choro...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/j.jneuroim.2007.05.005
更新日期：2007-07-01 00:00:00
abstract：:We investigated whether the sensory neuropeptide, calcitonin gene-related peptide (CGRP), could be synthesised by human lymphocytes. Our results indicate that in activated B-cells, there is a strong expression of CGRP gene transcripts, which is almost absent in resting cells. Since B-cells autocrinally produce NGF, th...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/s0165-5728(01)00475-1
更新日期：2002-02-01 00:00:00
abstract：:In multiple sclerosis, infiltrating T lymphocytes and perivascular microglia may initiate demyelinating lesions, but a role for antibody and complement in the ensuing inflammatory damage to myelin and oligodendrocytes is likely. In most tissues, ubiquitously expressed complement regulatory proteins prevent autologous ...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/s0165-5728(97)00241-5
更新日期：1998-04-01 00:00:00
abstract：:Ischemic stroke is one of the leading causes of mortality and morbidity in humans. During brain ischemia and the subsequent reperfusion that occurs with stroke, the generation of the so-called "proinflammatory" prostaglandin E(2) (PGE(2)) increases significantly. Therefore, interest is growing regarding the differenti...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/j.jneuroim.2009.01.015
更新日期：2009-03-31 00:00:00
abstract：:Loss-of-function mutations of DAP12 and TREM2 cause a recessively inherited disease PLOSL, manifesting in brain white matter. The genes of the DAP12-TREM2 signaling receptor are located on 19q13.12 and 6p21.1, to which linkage has been observed also in families affected by another immune-mediated demyelinating disease...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/j.jneuroim.2008.10.005
更新日期：2009-01-03 00:00:00
abstract：:To determine the contribution of vascular endothelial growth factor (VEGF) to cerebral edema formation in bacterial meningitis, we used a VEGF neutralizing antibody to block VEGF in rabbits, following induction of meningitis by intracisternal inoculation with 10(9) heat-killed pneumococci. At 8 h, cerebrospinal fluid ...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/j.jneuroim.2004.11.013
更新日期：2005-03-01 00:00:00
abstract：:Myasthenia gravis (MG) is an autoimmune disorder caused by antibody-mediated attack against skeletal muscle nicotinic acetylcholine receptor (AchR) at neuromuscular junction. A close relationship exists between heat shock proteins (HSPs) and numerous autoimmune diseases. HSPs are over expressed to protect the host aga...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/j.jneuroim.2013.02.001
更新日期：2013-04-15 00:00:00
abstract：:The concentrations of beta-endorphin and cholecystokinin were measured in fresh resting peripheral mononuclear cells obtained from rats and human subjects in basal conditions and after different pharmacological treatments. Both in the human and the rat, beta-endorphin concentrations in mononuclear cells, increased aft...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/0165-5728(91)90069-j
更新日期：1991-04-01 00:00:00
abstract：:PI-2301 is an immunomodulator that could be an alternative therapy for MS. A placebo-controlled, multiple-ascending dose, double-blind study was performed in patients with secondary-progressive MS. Treatment was given subcutaneously once weekly for 8 weeks, followed by a 4-week open-label treatment period with active ...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.jneuroim.2010.04.008
更新日期：2010-08-25 00:00:00
abstract：:To clarify the immunogenetic background of patients with immunoglobulin G (IgG)4 anti-neurofascin 155 (NF155) antibody-positive chronic inflammatory demyelinating polyneuropathy (CIDP), we genotyped the extended human leukocyte antigen (HLA) haplotypes in 22 Japanese patients with this disorder and compared them with ...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/j.jneuroim.2019.577139
更新日期：2020-02-15 00:00:00
abstract：:The expression of several cell type-specific markers was studied by indirect immunofluorescence in discontinuous BSA gradient fractionated and unfractionated mouse cerebellar cells cultured for 3 days in vitro from embryonic day 13 through postnatal day 9. Cell surface antigen NS-4 and tetanus toxin receptors are pres...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/0165-5728(81)90024-2
更新日期：1981-12-01 00:00:00
abstract：:To assess whether TNF-alpha causes inflammatory demyelination or axonal degeneration, we injected into rat sciatic nerve saline, 100 U and 1000 U of rhTNF-alpha and studied the electrophysiological and pathological effects. At day 1 electrophysiology showed a slight reduction of proximal compound muscle action potenti...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/s0165-5728(98)00229-x
更新日期：1999-02-01 00:00:00
abstract：:The aim of the present study was to define the cellular composition of ventricular, as compared with lumbar, cerebrospinal fluid (CSF) in patients with non-inflammatory neurological disorders (NIND). We addressed this issue by determining the cellular composition of lumbar CSF from patients with normal pressure hydroc...
journal_title：Journal of neuroimmunology
pub_type： 临床试验,杂志文章
doi：10.1016/j.jneuroim.2005.03.003
更新日期：2005-06-01 00:00:00
abstract：:Antibody responses to myelin antigens were analysed in 15 patients who developed acute neuroparalytic accidents (ANPA) during post-exposure rabies vaccination using a rabies vaccine prepared on brain tissues and in 30 individuals who were uneventfully vaccinated. High titers (> or = 100) of IgG and IgM antibodies to G...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/s0165-5728(98)00153-2
更新日期：1998-11-02 00:00:00
abstract：:We investigated whether microglia form gap junctions with themselves, or with astrocytes, oligodendrocytes, or neurons in vivo in normal mouse brains, and in pathological conditions that induce microglial activation - brain injury and a model of Alzheimer's disease. Although microglia are in close physical proximity t...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/j.jneuroim.2014.02.005
更新日期：2014-04-15 00:00:00
abstract：:The enzyme heme oxygenase-1 (HO-1) is reducing heme to the gaseous mediator carbon monoxide, to iron and the antioxidant biliverdin. The inducible expression of HO-1 is considered a protective cellular mechanism against reactive oxygen intermediates. Further, carbon monoxide (CO) is a regulator of cGMP synthesis, of N...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/s0165-5728(00)00352-0
更新日期：2000-10-02 00:00:00
abstract：:We aimed to validate the diagnostic utility of enzyme-linked immunosorbent assay (ELISA) for the detection of anti-neurofascin (NF) 155 antibody in 191 patients with chronic inflammatory demyelinating polyneuropathy (CIDP). Human NF155-based ELISA clearly distinguished between anti-NF155 antibody-positive and -negativ...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/j.jneuroim.2016.10.013
更新日期：2016-12-15 00:00:00
abstract：:The pharmacological profile and the anatomical localisation of muscarinic cholinergic receptor subtypes were studied in the pigeon bursa of Fabricius, using radioligand binding and autoradiographic techniques with [3H]quinuclidinyl benzilate (QNB) as a ligand. [3H]QNB was specifically bound to sections of bursa of Fab...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/0165-5728(96)00012-4
更新日期：1996-05-01 00:00:00
abstract：:The acetylcholine receptor (AChR) is the main target antigen in myasthenia gravis (MG), but about 15% of patients with typical immunologically mediated MG do not have detectable anti-AChR antibodies. Previous studies showed that plasma from these 'seronegative' patients (SNMG) reduced AChR function in the human AChR-e...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/0165-5728(95)00169-7
更新日期：1996-02-01 00:00:00
abstract：:Microglia cells are the principal immune effector elements of the brain responding to any pathological event. To elucidate the possible role of microglia in initial non-inflammatory demyelination in canine distemper virus (CDV) infection, microglia from experimentally CDV infected dogs were isolated ex vivo by density...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/j.jneuroim.2004.05.001
更新日期：2004-08-01 00:00:00
abstract：:Transection of the rat facial nerve leads to an intrinsic microglial reaction in the facial nucleus. In the present study, we have examined immunocytochemically the expression of cell adhesion molecules during this process. Resting microglia constitutively expressed the LFA-1 alpha and beta chain (CD11a and CD18) in t...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/0165-5728(93)90181-w
更新日期：1993-06-01 00:00:00
abstract：:Peroxisome proliferator-activated receptor (PPAR)-gamma agonists, including thiazolidinediones (TZDs) and 15-deoxy-Delta(12,14) prostaglandin J(2) (15d-PGJ(2)), have been shown to be effective in the treatment of experimental autoimmune encephalomyelitis (EAE), an animal model of multiple sclerosis (MS). This study ai...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/j.jneuroim.2004.12.015
更新日期：2005-04-01 00:00:00
abstract：:Fibrinolytic activity in the form of plasminogen activator (PA) was assessed using a histochemical fibrin slide technique in spinal cords of normal Lewis rats and rats with the cell-transferred form of experimental allergic encephalomyelitis (EAE). PA was localized exclusively to blood vessels. Vessels in the leptomen...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/0165-5728(90)90012-c
更新日期：1990-08-01 00:00:00
