Abstract：
:The expression of several cell type-specific markers was studied by indirect immunofluorescence in discontinuous BSA gradient fractionated and unfractionated mouse cerebellar cells cultured for 3 days in vitro from embryonic day 13 through postnatal day 9. Cell surface antigen NS-4 and tetanus toxin receptors are present at all ages studied. Thy-1 first detected on neurons with large cell bodies (Purkinje and/or Golgi Type II.neurons) onpostnatal day 3, but absent from all neurons with small cell bodies (granule, basket, and stellate cells). At all ages Thy-1 antigen is absent from astro- and oligodendroglia, and fibroblasts or fibroblast-like cells. Fibroblast-like cells express fibronectin at all ages studied. Astroglia expressing glial fibrillary acidic (GFA) protein are first detectable in cultures from 16-day-old embryos. Their number increases at later ages. At all ages studied fluorescein conjugated Ricinus communis agglutinin 120 brightly labels the cell surfaces of fibroblastic cells and large neurons, less brightly those of astrocytes, bu not those of small neurons. Oligodendroglia become detectable in cerebellar cultures from 16- and 17-day-old embryos maintained in vitro for 3 days using antibodies to 04 antigen and bovine corpus callosum, respectively. At embryonic ages BSA step gradient procedures do not result in enrichment of particular cell types as recognized by the available markers. From birth onward, however, enrichment of cell populations was obtained corresponding to the ones characterized at postnatal day 6 as described in the companion paper (Schnitzer and Schachner 1981b).
journal_name
J Neuroimmunoljournal_title
Journal of neuroimmunologyauthors
Schnitzer J,Schachner Mdoi
10.1016/0165-5728(81)90024-2subject
Has Abstractpub_date
1981-12-01 00:00:00pages
471-87issue
4eissn
0165-5728issn
1872-8421pii
0165-5728(81)90024-2journal_volume
1pub_type
杂志文章abstract：:In multiple sclerosis, infiltrating T lymphocytes and perivascular microglia may initiate demyelinating lesions, but a role for antibody and complement in the ensuing inflammatory damage to myelin and oligodendrocytes is likely. In most tissues, ubiquitously expressed complement regulatory proteins prevent autologous ...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/s0165-5728(97)00241-5
更新日期：1998-04-01 00:00:00
abstract：:CSF and serum levels of soluble Fas were studied in MS patients, in patients with various neurological diseases and in healthy controls. We did not detect differences in serum sFas levels between MS patients and controls. In CSF, despite sFas levels being similar in all patients studied, a statistically significant co...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/S0165-5728(97)00177-X
更新日期：1998-02-01 00:00:00
abstract：:To determine clinical features of neurologic disorders associated with anti-glutamic acid decarboxylase antibodies (anti-GAD-Ab), we examined titers and time-dependent changes of anti-GAD-Ab. Six patients, stiff person syndrome (2), cerebellar ataxia (1), limbic encephalitis (1), epilepsy (1), brainstem encephalitis (...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/j.jneuroim.2018.01.007
更新日期：2018-04-15 00:00:00
abstract：:Dendritic cells are the critical mediators of various immune responses and are the first line of defense against any infection including HIV. They play a major role in harboring HIV and the subsequent infection of T cells and passage of virus through the blood-brain barrier (BBB). The recently discovered DC-specific, ...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/j.jneuroim.2004.08.040
更新日期：2004-12-01 00:00:00
abstract：:Myelination in the central nervous system requires an accurate interplay between oligodendrocyte precursor cells (OPC) and axons. By as yet not fully understood mechanisms, OPC proliferate, migrate to the axon to be myelinated and finally differentiate into mature oligodendrocytes. The recent finding that OPC express ...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/j.jneuroim.2006.05.021
更新日期：2006-09-01 00:00:00
abstract：:HLA-DR, Iba1 and CD68 are widely used microglia markers in human tissue. However, due to differences in gene regulation, they may identify different activation stages of microglia. Here, we directly compared the expression of HLA-DR, Iba1 and CD68 in microglia with different phenotypes, ranging from ramified to amoebo...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/j.jneuroim.2017.04.007
更新日期：2017-08-15 00:00:00
abstract：:Myasthenia gravis (MG) is an autoimmune disorder caused by antibody-mediated attack against skeletal muscle nicotinic acetylcholine receptor (AchR) at neuromuscular junction. A close relationship exists between heat shock proteins (HSPs) and numerous autoimmune diseases. HSPs are over expressed to protect the host aga...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/j.jneuroim.2013.02.001
更新日期：2013-04-15 00:00:00
abstract：:The synthetic amino acid copolymer, copolymer 1 (Cop 1) induces T suppressor (Ts) lines/clones, which are confined to the Th2 pathway, cross react with myelin basic protein (MBP), but not with other myelin antigens on the level of Th2 cytokine secretion. Nevertheless, Cop 1 Ts cells inhibited the IL-2 response of a pr...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/s0165-5728(98)00166-0
更新日期：1998-11-02 00:00:00
abstract：:We evaluated the concentration of antineuronal antibodies in paired cerebrospinal fluid (CSF) and serum samples from 19 patients with central and peripheral paraneoplastic neurological syndromes (PNS), using an enzyme linked immunosorbent assay (ELISA) employing recombinant antineuronal antigens (HuD, Yo, Ri, CV2/CRMP...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/j.jneuroim.2006.11.008
更新日期：2007-02-01 00:00:00
abstract：:Functional studies of cerebrospinal fluid T lymphocytes during acute viral infections of the nervous system are rare. Recently, we had the opportunity to investigate the requirements for interferon-gamma (IFN-gamma) production of human in vivo activated (primary) cytotoxic T lymphocytes (CTL) generated during acute vi...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/0165-5728(91)90106-h
更新日期：1991-09-01 00:00:00
abstract：:The peroxisome proliferator-activated receptor gamma agonist pioglitazone is FDA-approved for treatment of type-2 diabetes due to insulin sensitizing effects. However pioglitazone has anti-inflammatory and neuroprotective effects, reduces glial and T-cell activation, and reduces signs in an animal model of multiple sc...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.jneuroim.2009.04.011
更新日期：2009-06-25 00:00:00
abstract：:The B7/CD28:CTLA-4 costimulatory pathway plays a critical role in determining the fate of immune responses (activation vs. down-regulation) and is a highly promising therapeutic target for treating autoimmune diseases. In this review, we highlight the mechanisms by which this costimulatory pathway operates emphasizing...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章,评审
doi：10.1016/s0165-5728(98)00058-7
更新日期：1998-08-14 00:00:00
abstract：:In this study we aimed to investigate whether treatment with an immune modulatory drug had an effect on the distribution of B cell subpopulations in patients with remitting-relapsing multiple sclerosis (RRMS). We investigated the first-line drugs glatiramer acetate, interferon-β and natalizumab. Our data show that the...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/j.jneuroim.2014.04.008
更新日期：2014-07-15 00:00:00
abstract：:Some patients with peripheral neuropathy and gammopathy have IgM monoclonal antibodies that react with the myelin-associated glycoprotein (MAG), some 20-26 kDa glycoproteins present only in the peripheral nervous system (PNS), and some acidic glycolipids that are also PNS-specific. This communication describes an inve...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/0165-5728(86)90114-1
更新日期：1986-04-01 00:00:00
abstract：:G-protein-coupled receptors (GPCR) play an important role in inflammation. Their responsiveness is regulated by G-protein-coupled receptor kinases (GRKs) and beta-arrestins. We show here that induction of experimental autoimmune encephalomyelitis (EAE) by myelin oligodendrocyte glycoprotein (MOG) resulted in a profoun...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/s0165-5728(03)00050-x
更新日期：2003-04-01 00:00:00
abstract：:Light and electron immunocytochemistry using antibodies recognising a class II major histocompatibility complex antigen, fibrin, fibronectin, albumin and factor VIII related antigen has been used to stain sections of spinal cord from normal guinea pigs and those with chronic relapsing experimental allergic encephalomy...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/0165-5728(89)90003-9
更新日期：1989-03-01 00:00:00
abstract：:To elucidate the possible involvement of the inducible nitric oxide synthase (iNOS) and NO in the development of lymphocytic choriomeningitis (LCM), the consequences of inhibition of iNOS by the inhibitor aminoguanidine was examined in mice following intracerebral infection with LCM virus (LCMV). Aminoguanidine admini...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/s0165-5728(96)00129-4
更新日期：1996-12-01 00:00:00
abstract：:Fatigue is a common "ghost" symptom in patients with multiple sclerosis (MS), an autoimmune disease mediated by T cells that target myelin antigens of the central nervous system. As fatigue has been associated with inflammatory states, its occurrence may negatively impact MS progression. The aim of this study was to e...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/j.jneuroim.2016.12.013
更新日期：2017-02-15 00:00:00
abstract：:We previously reported that the CD4+ suppressor cells (Ts) that regulate recovery of Lewis rats from experimental autoimmune encephalomyelitis (EAE) produce transforming growth factor-beta (TGF-beta). We also reported that TGF-beta downregulates interferon-gamma (IFN-gamma), but not interleukin-2 (IL-2) production, by...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/0165-5728(94)90131-7
更新日期：1994-04-01 00:00:00
abstract：:The overall goal of this study was to determine, during induction of experimental autoimmune myasthenia gravis (EAMG) in Lewis rats, the relative importance of acetylcholine receptor (AChR)-reactive helper T cells associated with one particular immunodominant fine specificity. Thus, experiments presented below were de...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/0165-5728(94)00159-l
更新日期：1995-03-01 00:00:00
abstract：:Indirect immunofluorescence and cell-type-specific markers were used to determine whether antibodies directed against specific neural cell types were present in patients with Alzheimer's disease (AD), other neurological diseases (OND) and normal controls. Sera and cerebrospinal fluid (CSF) were tested on either frozen...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/0165-5728(81)90013-8
更新日期：1981-03-01 00:00:00
abstract：:Autologous stem cell transplantation is being considered as treatment of severe refractory autoimmune disorders, including MS. Stem cell mobilization is achieved with granulocyte-colony stimulating factor (G-CSF), however, G-CSF administration resulted in cases of worsened clinical MS status. We studied autoreactive T...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/j.jneuroim.2005.11.012
更新日期：2006-03-01 00:00:00
abstract：:We previously reported that interleukin-4 (IL-4) inhibited proliferation of a human astrocytic cell line derived from non-neoplastic adult cortex. To determine whether this effect was receptor-associated and/or limited to only non-neoplastic astrocytes, we examined IL-4 responsiveness and receptor expression in human ...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/0165-5728(95)00055-7
更新日期：1995-07-01 00:00:00
abstract：:We investigated the role of IFN-gamma activated microglia in the passage of T lymphocytes across a monolayer of brain endothelial cells (EC) in vitro. Microglia isolated from Fisher 344 (F344) newborn rats were stimulated with IFN-gamma (100 U/ml) for 48 h. T lymphocytes primed with glioma cells were 51Cr-labeled, and...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/s0165-5728(97)00163-x
更新日期：1998-01-01 00:00:00
abstract：:Lymph node cells (LNC) from SJL/J mice immunized with either rat whole spinal cord (WSC) homogenate or rat myelin basic protein (MBP) in the presence of complete Freund's adjuvant were assayed for their in vitro proliferative response to MBP and whole or sonicated purified oligodendrocytes (OD), and astrocytes. WSC-im...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/0165-5728(89)90162-8
更新日期：1989-01-01 00:00:00
abstract：:This study aimed to determine the seasonal impact on the clinical onset of inflammatory neurological diseases of the central nervous system by analyzing the onset month with information on clinical manifestations in Japanese patients. As a result, patients with anti-aquaporin-4 antibodies (AQP4-IgG)-positive neuromyel...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章,多中心研究
doi：10.1016/j.jneuroim.2020.577431
更新日期：2020-12-15 00:00:00
abstract：:The prevalence of vascular dementia is increasing at an alarming rate. The Confirmation of the clinical diagnosis of vascular dementia depends on post-mortem examination of the brain. In our study, we investigated the vascular disease and neuroinflammation during vascular dementia. Our results showed a β-amyloid depos...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/j.jneuroim.2018.04.004
更新日期：2018-07-15 00:00:00
abstract：:The aberrant expression of B7 costimulatory molecules is involved in the pathogenesis of autoimmune diseases and overexpression of B7-1 was found in inflammatory multiple sclerosis (MS) lesions. We here report that costimulatory molecules B7-1 and B7-2 are expressed on cerebrospinal fluid (CSF) monocytes and B-lymphoc...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/s0165-5728(99)00012-0
更新日期：1999-04-01 00:00:00
abstract：:CD4+CD25+ regulatory T cells (Treg) transform the HIV-1 infected macrophage from a neurotoxic to a neuroprotective phenotype. This was demonstrated previously in a murine model of HIV-1 encephalitis induced by intracranial injection of HIV-1/vesicular stomatitis virus-infected bone marrow macrophages. In this report, ...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/j.jneuroim.2010.08.014
更新日期：2011-01-01 00:00:00
abstract：:We describe here the methods by which enriched populations of oligodendrocytes were isolated from adult porcine brains using Percoll density gradient centrifugation and their immunological properties analyzed by a fluorescence-activated cell sorter (FACS). The analyses by immunofluorescence microscopy and FACS indicat...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/0165-5728(86)90049-4
更新日期：1986-11-01 00:00:00
