Abstract：
:Cell-intact patch-clamp recording was used to determine the electrophysiological responses of sheep anterior pituitary gonadotropes to stimulation with gonadotropin-releasing hormone (GnRH). Cells were identified prior to recording by reverse haemolytic plaque assay (RHPA), or using morphological criteria in preparations enriched in gonadotropes by Percoll density gradient centrifugation. Most cells identified by RHPA did not generate action potentials, and responses to GnRH were inconsistent. The majority of gonadotropes in enriched preparations however spontaneously generated action potentials requiring the entry of both extracellular Na+ and Ca2+, and involving tetraethylammonium-sensitive K+ channels. Two-minute GnRH application (10(-7) M) evoked a characteristic sequence of changes in action potential generation. The immediate response was an inhibition of action potentials, followed by a recovery of these events, with a progressive decline in amplitude over about 10 min. The cells then remained quiescent for up to 1 h. The results indicate that GnRH may evoke an initial hyperpolarization involving Ca(2+)-dependent K+ channels, followed by a sustained depolarizing response, with consequent inactivation of action potential generation.
journal_name
Neuroendocrinologyjournal_title
Neuroendocrinologyauthors
Heyward PM,Chen C,Clarke IJdoi
10.1159/000126605subject
Has Abstractpub_date
1993-12-01 00:00:00pages
646-54issue
6eissn
0028-3835issn
1423-0194journal_volume
58pub_type
杂志文章abstract：:The effect of aging on the release of gonadotropin-releasing hormone (GnRH) in vitro and of luteinizing hormone (LH) both in vivo and in vitro in ovariectomized (Ovx) rats was studied. Old (21-24 months) and young (3-4 months) rats were Ovx before use. They were injected subcutaneously with estradiol benzoate (25 micr...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000125562
更新日期：1990-08-01 00:00:00
abstract：:This study was designed to investigate the effects of penfluridol, a potent neuroleptic calmodulin inhibitor, on basal and secretagogue-stimulated secretion of thyroid-stimulating hormone (TSH), growth hormone (GH), and luteinizing hormone (LH). The drug had no effect on basal TSH or LH release, but decreased GH relea...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000123548
更新日期：1983-09-01 00:00:00
abstract：:Neurons in the adult rat ventromedial hypothalamic nucleus (VMN, 4-6 neurons per brain; 3-7 brains per group) were studied under various hormonal conditions using the single-section Golgi impregnation technique. Intact rats of both sexes and ovariectomized females treated with oil, estrogen or estrogen and progesteron...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000125387
更新日期：1990-05-01 00:00:00
abstract：:In pituitary cells, c-fos transcription induced by releasing hormones and growth factors results from enhanced initiation of transcription, and sustained elongation of transcripts beyond the first intron. We studied the regulatory role of the first intron of the mouse c-fos gene for the control of its transcription in...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000054606
更新日期：2000-12-01 00:00:00
abstract：:The effect of sonicated suspensions of phosphatidylserine (PS), an acidic phospholipid, was investigated on prolactin secretion utilizing in vivo and in vitro approaches. Acute and chronic administration of PS reduced plasma prolactin levels during different phases of circadian rhythms. The phospholipid was able to in...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000123261
更新日期：1981-12-01 00:00:00
abstract：BACKGROUND:The appetite-stimulating hormone ghrelin is a fundamental regulator of human energy metabolism. A series of studies support the notion that long-term appetite and weight regulation may be already programmed in early life and it could be demonstrated that the intrauterine environment affects the ghrelin syste...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000363325
更新日期：2014-01-01 00:00:00
abstract：:The ability of dopamine receptor antagonists to stimulate prolactin release in rats with medial basal hypothalamic lesions was investigated. Starting levels of prolactin were elevated to approximately 400 ng/ml in animals in which the tuberoinfundibular dopaminergic neurons were completely destroyed by the lesion. In ...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000123188
更新日期：1981-06-01 00:00:00
abstract：:In situ hybridization was used to examine the effects of amygdala kindling on the expression of mRNAs for mineralocorticoid receptors (MR) and glucocorticoid receptors (GR) in the rat hippocampus. Kindling increased MR mRNA levels in the dentate gyrus of the hippocampus 4 h after the last seizure, and these levels rem...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000126691
更新日期：1994-05-01 00:00:00
abstract：:It has been well documented that interleukin-1beta (IL-1beta) is a major mediator for recruiting the hypothalamo-pituitary-adrenal (HPA) axis following infectious disease. The recent localization of IL-1beta receptors in neurons of the hippocampus provides further support for the role of IL-1beta as a neurotransmitter...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000054405
更新日期：1999-02-01 00:00:00
abstract：:Peptidyl-glycine alpha-amidation activity has been detected in human cerebrospinal fluid (CSF) and in several regions of the central nervous system. Activity was monitored by measuring conversion of mono-125I-D-Tyr-Val-Gly into mono-125I-D-Tyr-Val-NH2. The alpha-amidation activity in CSF is dependent on molecular oxyg...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000124223
更新日期：1985-12-01 00:00:00
abstract：:The effects of the dopamine (DA) receptor antagonist metoclopramide on the plasma thyroid stimulating hormone (TSH) and prolactin (PRL) levels were studied in 8 patients with subclinical hypothyroidism (defined as absence of clinical signs of hypothyroidism with normal thyroid hormone levels, normal or slightly increa...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000124603
更新日期：1986-01-01 00:00:00
abstract：:Previous studies have shown that prepubertal olfactory bulbectomy will prevent the testicular regression associated with short photoperiod in golden hamsters. The gonadal regression which normally occurs in hamsters on short photoperiod is known to be due in part to an increased responsiveness of the reproductive neur...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000124839
更新日期：1987-10-01 00:00:00
abstract：:After the combined treatment with dexamethasone, morphine and pentobarbitone, the electrical stimulation in the rat tuber cinereum induces a rise in plasma corticosterone (CS) level, probably by direct stimulation of the corticoliberin-producing neural elements and the consecutive ACTH release. We have attempted to lo...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000122803
更新日期：1978-01-01 00:00:00
abstract：BACKGROUND:Activation of somatostatin receptors (sstr1-5) by somatostatin and its analogues exerts an inhibitory effect on hormone secretion and provides the basis for the treatment of a range of endocrine diseases such as acromegaly, Cushing's disease and neuroendocrine tumors (NET). The lack of well-characterized com...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000330616
更新日期：2012-01-01 00:00:00
abstract：BACKGROUND:Gastroenteropancreatic neuroendocrine tumors (GEP-NETs) express insulin-like growth factor (IGF)-related factors [IGF1, IGF2; insulin receptor (IR)-A, IR-B; IGF-binding protein (IGFBP) 1-3] as well as somatostatin (SSTRs) and dopamine D2 receptors (D2Rs). OBJECTIVES:To (1) compare mRNA expression of IGF-rel...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000444280
更新日期：2016-01-01 00:00:00
abstract：:Three control point enzymes of the Embden-Meyerhof pathway, hexokinase (HK), phosphofructokinase (PFK) and pyruvate kinase (PK) were measured by quantitative histochemical methods in individual hypothalamic nuclei of adult neonatally androgenized female rats. HK activity was significantly increased in anterior hypotha...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000122715
更新日期：1977-01-01 00:00:00
abstract：:We have suggested that gamma-aminobutyric acid (GABA) in the hypothalamus plays a tonic inhibitory role in the control of the luteinizing hormone (LH) release in intact male rats. To assess whether feedback from the testis alters the inhibitory GABAergic tone in the medial preoptic area (MPO) of male rats, an in vivo ...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000066625
更新日期：2002-11-01 00:00:00
abstract：:The influence of catecholamines (CA) on hypothalamic somatostatin (HPT-SRIF) was investigated in rats by using several drugs which interfere with brain CA metabolism. Depletion of brain CA stores by alpha-methyl-rho-tyrosine (AMT) increased HPT-SRIF, while augmented brain CA levels following L-dopa administration decr...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000123374
更新日期：1982-01-01 00:00:00
abstract：:The growth hormone secretagogue receptor subtype 1a (GHSR-1a) is involved in biological actions of ghrelin by triggering intracellular second messengers coupled to heterotrimeric G-protein complex involving Galpha(q/11). Adenosine is a partial agonist of the GHSR-1a, binding to a binding pocket distinct from the one d...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000076042
更新日期：2004-01-01 00:00:00
abstract：:Pregnancy and parturition are associated with opioid-mediated elevations in maternal pain thresholds. This analgesia is subserved by a spinal cord dynorphin/kappa-opiate receptor system. During gestation, elevated pain thresholds are paralleled by a significant increase in the content of dynorphin (1-17 and 1-8) in th...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000126555
更新日期：1993-09-01 00:00:00
abstract：:The relationship between concentrations of vasopressin in plasma and cerebrospinal fluid (CSF) was examined under basal and hypoxic conditions in unanesthetized and unrestrained adult female sheep. Under basal conditions mean (+/- SE) concentration of vasopressin (4.1 +/- 0.3 pg/ml) in CSF was significantly (p less th...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000123933
更新日期：1984-06-01 00:00:00
abstract：:Neuroendocrine mechanisms governing growth hormone (GH) secretion are sensitive to nutritional status since the normal pulsatile pattern of GH release is disrupted during conditions of food deprivation or malnutrition. A reasonable hypothesis for this occurrence is the alteration of somatostatin and GH-releasing hormo...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000127172
更新日期：1997-02-01 00:00:00
abstract：:Parturition is initiated in sheep by an increase in the activity of the fetal hypothalamus-pituitary-adrenal (HPA) axis. Prostaglandins, known to augment the activity of this endocrine axis, have long been proposed as involved in the initiation of paturition. We have previously demonstrated that endogenously produced ...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000054548
更新日期：2000-05-01 00:00:00
abstract：:Vasopressin and oxytocin modulate memory processes which effects are dissociated from the typical peripheral endocrine effects of these neuropeptides. Recently, vasopressin has been implicated in the regulation of body temperature. In view of this, experiments were designed to determine whether the antipyretic effect ...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000123554
更新日期：1983-10-01 00:00:00
abstract：AIM:To extensively explore microRNA expression profiles in lung carcinoids in correlation with clinical and pathological features. METHODS:A PCR-based array was employed in the screening phase to analyze 752 microRNAs in a discovery set of 12 lung carcinoids, including 6 typical (3 with lymph node metastasis) and 6 at...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000381454
更新日期：2015-01-01 00:00:00
abstract：:Changes in antidiuretic substance (i.e., vasopressin) release from the cut pituitary stalk and the intact pituitary gland were observed during electrical stimulation of amygdala medial nucleus. Electrical pulses of 4 or 36 cycles per second (cps) were used and in both cases the same energy (about 2.160 nanowatt second...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000122349
更新日期：1975-01-01 00:00:00
abstract：:In addition to the magnocellular hypothalamic nuclei, arginine vasopressin (AVP)-containing neurons have also been identified in limbic structures, including the hippocampus and amygdala. In the present study, we compared the qualitative properties of the in vitro release of AVP from the dissected hypothalamus with th...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000126661
更新日期：1994-03-01 00:00:00
abstract：:The diuretic action of two new narcotic antagonists, oxilorphan and butorphanol, was studied in rats heterozygous for hereditary hypothalmic diabetes insipidus. Both drugs caused a prompt increase in urine volume and a decrease in urine osmolality with an associated decrease in urinary ADH excretion. The effects appea...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000122444
更新日期：1975-01-01 00:00:00
abstract：:Knowledge of specific neurotransmitters as well as the pathways and mechanisms regulating appetite in ruminants that continually graze, such as sheep, is incomplete. Although fundamentally agouti-related protein (AGRP) has a similar function across species to increase food intake, the regulation of AGRP may vary acros...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000082735
更新日期：2004-01-01 00:00:00
abstract：:Immobilization of adult male rats resulted in concomitant, significant releases of prolactin (PRL) and oxytocin (OT). Naloxone (0.2 mg/kg, i.p.) administration 30 min prior to initiation of restraint resulted in a significant diminution of the PRL response to stress. On the other hand, a significant augmentation of OT...
journal_title：Neuroendocrinology
pub_type： 杂志文章
doi：10.1159/000124053
更新日期：1985-01-01 00:00:00
