Agonist-specific coupling of growth hormone secretagogue receptor type 1a to different intracellular signaling systems. Role of adenosine.

Abstract:

:The growth hormone secretagogue receptor subtype 1a (GHSR-1a) is involved in biological actions of ghrelin by triggering intracellular second messengers coupled to heterotrimeric G-protein complex involving Galpha(q/11). Adenosine is a partial agonist of the GHSR-1a, binding to a binding pocket distinct from the one described for ghrelin. This suggests a variety of functions for the poorly understood GHSR1a receptor. In this work, a sequential analysis of the pathways involved in the regulation of GHSR-1a signaling was undertaken to characterize the intracellular calcium mobilization that is observed following adenosine binding. The results showed that adenosine induced, in a dose-dependent manner, a calcium mobilization from IP(3)-sensitive intracellular stores since the IP(3) receptor blocker 2-APB was able to suppress the calcium response. However, adenosine did not show any effect in the formation of inositol phosphates. The calcium-mobilizing activity was blocked after preincubation of cells with CTX, the inhibitor of adenylate cyclase MDL-12,330A and the protein kinase A blocker H-89. Furthermore, the administration of adenosine stimulated cAMP production. Based on the experimental data, a signaling pathway is proposed involving adenylate cyclase and protein kinase A, which causes phosphorylation of the IP(3) receptor, with a cross-talk between the signaling pathways activated by ghrelin and adenosine. The data described in this report suggest that GHSR-1a is able to activate different intracellular second-messenger systems depending on the agonist that activates it. The regulation of the ghrelin-activated earliest signaling pathways by adenosine may have unexpected implications in the GHSR-1a actions.

journal_name

Neuroendocrinology

journal_title

Neuroendocrinology

authors

Carreira MC,Camiña JP,Smith RG,Casanueva FF

doi

10.1159/000076042

subject

Has Abstract

pub_date

2004-01-01 00:00:00

pages

13-25

issue

1

eissn

0028-3835

issn

1423-0194

pii

76042

journal_volume

79

pub_type

杂志文章
  • Autoradiographic identification of prolactin binding sites in rat median eminence.

    abstract::Binding sites for prolactin were localized and quantified in the female rat brain by in vitro autoradiographic analysis with iodine-125-labeled ovine prolactin. Following incubation, labeled prolactin bound preferentially to the median eminence and choroid plexus of the lateral and third ventricles. The addition of ex...

    journal_title:Neuroendocrinology

    pub_type: 杂志文章

    doi:10.1159/000125182

    authors: Barton AC,Lahti RA,Piercey MF,Moore KE

    更新日期:1989-06-01 00:00:00

  • Beta-adrenergic receptors in the premammillary nucleus mediate the inhibition of LH release evoked by locus ceruleus stimulation.

    abstract::The neurotransmitter and andrenoceptors involved in the inhibition of luteinizing hormone (LH) release induced by stimulating the locus ceruleus nucleus (LC) have been characterized in ovariectomized, estrogen-primed rats in which the release of LH was triggered by electrical stimulation of the medial preoptic area. B...

    journal_title:Neuroendocrinology

    pub_type: 杂志文章

    doi:10.1159/000123858

    authors: Dotti C,Taleisnik S

    更新日期:1984-01-01 00:00:00

  • VERTEBRAL FRACTURES ASSOCIATED WITH SPINAL SAGITTAL IMBALANCE AND QUALITY OF LIFE IN ACROMEGALY: A RADIOGRAPHIC STUDY WITH EOS 2D/3D TECHNOLOGY.

    abstract:INTRODUCTION:Acromegaly is commonly complicated by arthropathy and skeletal fragility with high risk of vertebral fractures (VFs). OBJECTIVE:This study aimed to assess whether VFs may be associated with sagittal spine deformities, arthropathy, impaired quality of life (QoL), pain and disability. METHODS:Thirty-eight ...

    journal_title:Neuroendocrinology

    pub_type: 杂志文章

    doi:10.1159/000511811

    authors: Cellini M,Biamonte E,Mazza M,Trenti N,Ragucci P,Milani D,Ferrante E,Rossini Z,Lavezzi E,Sala E,Mantovani G,Arosio M,Fornari M,Balzarini L,Lania AG,Mazziotti G

    更新日期:2020-09-25 00:00:00

  • Neuroanatomical localization of sex steroid-concentrating cells in the Japanese quail (Coturnix japonica): autoradiography with [3H]-testosterone, [3H]-estradiol, and [3H]-dihydrotestosterone.

    abstract::Steroid autoradiography was undertaken to determine the neuroanatomical loci which might be involved in the activation of steroid-sensitive behaviors in the Japanese quail (Coturnix japonica). Male and female quail were either surgically gonadectomized or photically regressed and implanted with androgen or estrogen to...

    journal_title:Neuroendocrinology

    pub_type: 杂志文章

    doi:10.1159/000125091

    authors: Watson JT,Adkins-Regan E

    更新日期:1989-01-01 00:00:00

  • Subfornical organ connections with septal neurons projecting to the median eminence.

    abstract::We have examined the effect of electrical stimulation in the subfornical organ (SFO) on the activity of neurons in the medial septum and diagonal band of Broca (MS-DBB) which project to the median eminence in the rat. Previous studies have suggested that septal neurons with such projections are luteinizing hormone rel...

    journal_title:Neuroendocrinology

    pub_type: 杂志文章

    doi:10.1159/000124991

    authors: Donevan SD,Ferguson AV

    更新日期:1988-07-01 00:00:00

  • Estrogen modulation of mu-opioid receptor-stimulated [35S]-GTP-gamma-S binding in female rat brain visualized by in vitro autoradiography.

    abstract::The mu-opioid receptor (OR) is involved in several aspects of female reproductive neuroendocrinology, such as the control of gonadotropin release and the display of lordosis behavior. Even though the neuroendocrine events modulated by mu-ORs are steroid hormone-dependent, few studies have shown how steroid hormones su...

    journal_title:Neuroendocrinology

    pub_type: 杂志文章

    doi:10.1159/000065953

    authors: Acosta-Martinez M,Etgen AM

    更新日期:2002-10-01 00:00:00

  • An early pineal-induced suppression of pituitary prolactin mRNA levels in light-deprived male hamsters.

    abstract::Light deprivation by blinding or exposure to short photoperiod results in depressed prolactin (PRL) cell activity and gonadal regression in the male Syrian hamster after at least 42-56 days of treatment, and both of these events can be prevented if pineal removal accompanies light deprivation. In the present study, we...

    journal_title:Neuroendocrinology

    pub_type: 杂志文章

    doi:10.1159/000125272

    authors: Massa JS,Blask DE

    更新日期:1989-11-01 00:00:00

  • ACTH release after tuberal electrical stimulation in rats with various cuts around the medial basal hypothalamus.

    abstract::After the combined treatment with dexamethasone, morphine and pentobarbitone, the electrical stimulation in the rat tuber cinereum induces a rise in plasma corticosterone (CS) level, probably by direct stimulation of the corticoliberin-producing neural elements and the consecutive ACTH release. We have attempted to lo...

    journal_title:Neuroendocrinology

    pub_type: 杂志文章

    doi:10.1159/000122803

    authors: Makara GB,Stark E,Palkovits M

    更新日期:1978-01-01 00:00:00

  • Hypoprolactinemia induced by hypophysectomy and long-term bromocriptine treatment decreases tuberoinfundibular dopaminergic neuronal activity and the responsiveness of these neurons to prolactin.

    abstract::The effect of long-term decreases in circulating concentrations of prolactin was determined on the responsiveness of tuberoinfundibular dopamine (DA) neurons to this hormone. The activity of these neurons in ovariectomized rats was estimated by measuring the rate of DA synthesis (DOPA accumulation after the administra...

    journal_title:Neuroendocrinology

    pub_type: 杂志文章

    doi:10.1159/000124100

    authors: Demarest KT,Riegle GD,Moore KE

    更新日期:1985-05-01 00:00:00

  • Regulation of His-dTrp-Ala-Trp-dPhe-Lys-NH2 (GHRP-6)-induced GH secretion in the rat.

    abstract::His-dTrp-Ala-Trp-dPhe,Lys-NH2(GHRP-6) is a synthetic compound that releases GH in a dose-response and specific manner in several species and that may well be related to an endogenous compound of similar structure. The aim of this study was to investigate the in vivo GH responses to GHRP-6 in pentobarbital anesthetized...

    journal_title:Neuroendocrinology

    pub_type: 杂志文章

    doi:10.1159/000126366

    authors: Mallo F,Alvarez CV,Benitez L,Burguera B,Coya R,Casanueva FF,Dieguez C

    更新日期:1993-01-01 00:00:00

  • Vasectomy modulates the local effect of intratesticular opioids on the gonad of immature rats.

    abstract::Previous studies indicate that 7 days after testicular administration of naloxone, serum testosterone (T) concentration and basal T secretion in vitro decrease significantly. In neonatal rats, short-term (2 h) intratesticular treatment with 0.3 micrograms (D-Met2-Pro5)-enkephalinamide suppresses steroidogenesis. In th...

    journal_title:Neuroendocrinology

    pub_type: 杂志文章

    doi:10.1159/000126242

    authors: Gerendai I,Nemeskéri A,Csernus V

    更新日期:1992-09-01 00:00:00

  • Increased number of corticotropin-releasing hormone expressing neurons in the hypothalamic paraventricular nucleus of patients with multiple sclerosis.

    abstract::Observations in experimental allergic encephalomyelitis (EAE), a model for multiple sclerosis (MS), have indicated that a low activity of the hypothalamo-pituitary-adrenal (HPA) system is accompanied by a high susceptibility for EAE in rat strains and that elevated corticosteroid levels are necessary for spontaneous r...

    journal_title:Neuroendocrinology

    pub_type: 杂志文章

    doi:10.1159/000126989

    authors: Purba JS,Raadsheer FC,Hofman MA,Ravid R,Polman CH,Kamphorst W,Swaab DF

    更新日期:1995-07-01 00:00:00

  • ADH release from cut pituitary stalk and intact pituitary gland during amygdala stimulation at various frequencies in rats.

    abstract::Changes in antidiuretic substance (i.e., vasopressin) release from the cut pituitary stalk and the intact pituitary gland were observed during electrical stimulation of amygdala medial nucleus. Electrical pulses of 4 or 36 cycles per second (cps) were used and in both cases the same energy (about 2.160 nanowatt second...

    journal_title:Neuroendocrinology

    pub_type: 杂志文章

    doi:10.1159/000122349

    authors: Tomas T

    更新日期:1975-01-01 00:00:00

  • Photoperiodic dependence of seasonal changes in pituitary content of melanocyte-stimulating hormone.

    abstract::In the Siberian hamster the pituitary content of bioassayable MSH has been shown to be seasonally variable, being greater during the long days of spring and summer than during the short days of autumn and winter. Similar changes in the pituitary content of both bioassayable MSH and immunoreactive alpha MSH can be indu...

    journal_title:Neuroendocrinology

    pub_type: 杂志文章

    doi:10.1159/000123019

    authors: Logan A,Weatherhead B

    更新日期:1980-05-01 00:00:00

  • Quantitative histochemical studies of the hypothalamus. Control point enzymes following androgen sterilization.

    abstract::Three control point enzymes of the Embden-Meyerhof pathway, hexokinase (HK), phosphofructokinase (PFK) and pyruvate kinase (PK) were measured by quantitative histochemical methods in individual hypothalamic nuclei of adult neonatally androgenized female rats. HK activity was significantly increased in anterior hypotha...

    journal_title:Neuroendocrinology

    pub_type: 杂志文章

    doi:10.1159/000122715

    authors: Packman PM,Bragdon MJ,Boshans RL

    更新日期:1977-01-01 00:00:00

  • Naloxone-induced dissociation of oxytocin and prolactin releases.

    abstract::Immobilization of adult male rats resulted in concomitant, significant releases of prolactin (PRL) and oxytocin (OT). Naloxone (0.2 mg/kg, i.p.) administration 30 min prior to initiation of restraint resulted in a significant diminution of the PRL response to stress. On the other hand, a significant augmentation of OT...

    journal_title:Neuroendocrinology

    pub_type: 杂志文章

    doi:10.1159/000124053

    authors: Samson WK,McDonald JK,Lumpkin MD

    更新日期:1985-01-01 00:00:00

  • Effects of centrally administered prolactin upon mammary contractility in anesthetized lactating rats.

    abstract::The possibility was investigated that centrally administered rat prolactin (PRL), i.e., intracerebroventricularly (ICV) or intrathecally (IT), may influence mammary contractility in urethane-anesthetized lactating rats. In addition to the monomer (23 kD PRL), the 16- and 7-kD PRL fragments were also tested and their e...

    journal_title:Neuroendocrinology

    pub_type: 杂志文章

    doi:10.1159/000127006

    authors: Morales MT,Mena F

    更新日期:1995-08-01 00:00:00

  • The actions of prostaglandin E2, naloxone and testosterone on starvation-induced suppression of luteinizing hormone-releasing hormone and luteinizing-hormone secretion. In vitro and in vivo studies.

    abstract::In man and other mammals, starvation is accompanied by a severe suppression of luteinizing hormone-releasing hormone (LHRH) and luteinizing-hormone (LH) secretion, which is caused by unknown alterations in hypothalamic functions. Prostaglandin E2 (PGE2), endorphins and testosterone (T) are know to be strongly involved...

    journal_title:Neuroendocrinology

    pub_type: 杂志文章

    doi:10.1159/000124034

    authors: Küderling I,Dorsch G,Warnhoff M,Pirke KM

    更新日期:1984-12-01 00:00:00

  • IL-1 beta potentiates the acetylcholine-induced release of vasopressin from the hypothalamus in vitro, but not from the amygdala.

    abstract::In addition to the magnocellular hypothalamic nuclei, arginine vasopressin (AVP)-containing neurons have also been identified in limbic structures, including the hippocampus and amygdala. In the present study, we compared the qualitative properties of the in vitro release of AVP from the dissected hypothalamus with th...

    journal_title:Neuroendocrinology

    pub_type: 杂志文章

    doi:10.1159/000126661

    authors: Raber J,Pich EM,Koob GF,Bloom FE

    更新日期:1994-03-01 00:00:00

  • Corticotrophin-releasing factor and arginine vasopressin fibre projections to the median eminence of fetal sheep.

    abstract::Using specific antibodies to corticotrophin-releasing factor (CRF) and arginine vasopressin (AVP) we have immunocytochemically located cells and fibres containing these peptides in sheep fetuses. CRF neurones were first detected, in low frequency, in the paraventricular nuclei of fetuses of 90 days of gestation (term ...

    journal_title:Neuroendocrinology

    pub_type: 杂志文章

    doi:10.1159/000124860

    authors: Levidiotis M,Oldfield B,Wintour EM

    更新日期:1987-11-01 00:00:00

  • A detailed characterization of prolactin secretion patterns during daylight in individual cycling and pseudopregnant rats.

    abstract::In order to characterize plasma prolactin (Prl) patterns during the estrous cycle and pseudopregnancy (PSP), I collected sequential blood samples between 8.00 and 19.00 h at 1-hour, 1.5-min or 1-min intervals from conscious, unrestrained female rats. During the afternoon of proestrus and estrus a single Prl surge was ...

    journal_title:Neuroendocrinology

    pub_type: 杂志文章

    doi:10.1159/000123247

    authors: Wiersma J

    更新日期:1981-11-01 00:00:00

  • Subfornical organ efferents influence the excitability of neurohypophyseal and tuberoinfundibular paraventricular nucleus neurons in the rat.

    abstract::Electrical stimulation in the subfornical organ (SFO) alters the excitability of antidromically identified paraventricular nucleus neurons. Extracellular recordings demonstrate that the dominant effect of single stimuli delivered to the SFO on neurohypophyseal oxytocin and vasopressin containing neurons is an increase...

    journal_title:Neuroendocrinology

    pub_type: 杂志文章

    doi:10.1159/000124015

    authors: Ferguson AV,Day TA,Renaud LP

    更新日期:1984-11-01 00:00:00

  • Effect of interleukin-1 on ACTH and corticosterone secretion in dexamethasone and adrenalectomized pretreated male rats.

    abstract::The present study was designed to investigate the role of glucocorticoids in mediating the stimulatory effect of interleukin-1 (IL-1) upon the adrenocortical (AC) axis. Intact male rats were injected with either vehicle or dexamethasone (DEX) (5-50 micrograms/100 g b.w.) and were subsequently exposed to ether stress o...

    journal_title:Neuroendocrinology

    pub_type: 杂志文章

    doi:10.1159/000125294

    authors: Weidenfeld J,Abramsky O,Ovadia H

    更新日期:1989-12-01 00:00:00

  • The History of Acromegaly.

    abstract::Pierre Marie coined the term 'acromegaly' in 1886 and linked it to a distinct clinical disease with a characteristic clinical picture. However, Pierre Marie was not the first physician to give a full record of the clinical picture of acromegaly; others had preceded him, like the Dutch physician Johannes Wier. After Ma...

    journal_title:Neuroendocrinology

    pub_type: 杂志文章,评审

    doi:10.1159/000371808

    authors: de Herder WW

    更新日期:2016-01-01 00:00:00

  • Comparison of chemotherapeutic treatment outcomes of advanced extrapulmonary neuroendocrine carcinomas and advanced small-cell lung carcinoma.

    abstract:BACKGROUND:The chemotherapy for small-cell lung carcinoma (SCLC) has been adopted for advanced extrapulmonary neuroendocrine carcinomas (EP-NECs). The aim of this study was to clarify the efficacy of standard SCLC regimens when used to treat EP-NECs and to compare the outcome with that for SCLC. METHODS:We reviewed th...

    journal_title:Neuroendocrinology

    pub_type: 杂志文章

    doi:10.1159/000338794

    authors: Terashima T,Morizane C,Hiraoka N,Tsuda H,Tamura T,Shimada Y,Kaneko S,Kushima R,Ueno H,Kondo S,Ikeda M,Okusaka T

    更新日期:2012-01-01 00:00:00

  • A role for agouti-related protein in appetite regulation in a species with continuous nutrient delivery.

    abstract::Knowledge of specific neurotransmitters as well as the pathways and mechanisms regulating appetite in ruminants that continually graze, such as sheep, is incomplete. Although fundamentally agouti-related protein (AGRP) has a similar function across species to increase food intake, the regulation of AGRP may vary acros...

    journal_title:Neuroendocrinology

    pub_type: 杂志文章

    doi:10.1159/000082735

    authors: Wagner CG,McMahon CD,Marks DL,Daniel JA,Steele B,Sartin JL

    更新日期:2004-01-01 00:00:00

  • The luteinizing hormone but not the cortisol response to arginine vasopressin is prevented by naloxone and a corticotropin-releasing hormone antagonist in the ovariectomized rhesus monkey.

    abstract::In the primate, arginine vasopressin (AVP) is known to activate the hypothalamo-pituitary-adrenal axis and to inhibit LH secretion. In the present study, we investigate the role of the endogenous opioid peptides and corticotropin-releasing hormone (CRH) in these processes. Adult ovariectomized rhesus monkeys bearing a...

    journal_title:Neuroendocrinology

    pub_type: 杂志文章

    doi:10.1159/000127121

    authors: Xiao E,Xia-Zhang L,Thornell D,Shalts E,Ferin M

    更新日期:1996-09-01 00:00:00

  • Glucocorticoids increase excitotoxic injury and inflammation in the hippocampus of adult male rats.

    abstract:BACKGROUND/AIMS:Stress exacerbates neuron loss in many CNS injuries via the actions of adrenal glucocorticoid (GC) hormones. For some injuries, this GC endangerment of neurons is accompanied by greater immune cell activation in the CNS, a surprising outcome given the potent immunosuppressive properties of GCs. METHODS...

    journal_title:Neuroendocrinology

    pub_type: 杂志文章

    doi:10.1159/000367849

    authors: Sorrells SF,Munhoz CD,Manley NC,Yen S,Sapolsky RM

    更新日期:2014-01-01 00:00:00

  • Decreased stimulation of adenylate cyclase by growth hormone-releasing factor in the anterior pituitary of old rats.

    abstract::Adenylate cyclase activity was studied in anterior pituitary homogenates from young adult (6 months) and old (20 and 24 months) male rats. Basal, NaF-, GTP-, 5'-guanylimidodiphosphate (Gpp(NH)p)- and forskolin-stimulated activities were similar in the three groups, implying that the stimulatory guanyl nucleotide regul...

    journal_title:Neuroendocrinology

    pub_type: 杂志文章

    doi:10.1159/000124682

    authors: Robberecht P,Gillard M,Waelbroeck M,Camus JC,De Neef P,Christophe J

    更新日期:1986-01-01 00:00:00

  • Demonstration of contacts between neurons staining for LHRH in the preoptic area of the rat brain.

    abstract::It has been recently demonstrated that luteinizing hormone-releasing hormone (LHRH) can inhibit LHRH release by the hypothalamus, suggesting an ultrashort feedback of LHRH on LHRH neurons. In order to test this hypothesis, immunocytochemical localization of LHRH was conducted in the preoptic area of the rat brain. Con...

    journal_title:Neuroendocrinology

    pub_type: 杂志文章

    doi:10.1159/000124861

    authors: Pelletier G

    更新日期:1987-11-01 00:00:00