Abstract:
:The mu-opioid receptor (OR) is involved in several aspects of female reproductive neuroendocrinology, such as the control of gonadotropin release and the display of lordosis behavior. Even though the neuroendocrine events modulated by mu-ORs are steroid hormone-dependent, few studies have shown how steroid hormones such as estrogen and/or progesterone can affect mu-OR function. Therefore, the present study investigated if in vivo estrogen or estrogen plus progesterone treatment of ovariectomized (OVX) rats affects mu-OR coupling to its G proteins. We used autoradiographic analysis of agonist-stimulated [(35)S]-GTPgammaS binding, in which brain sections were incubated in the presence or absence of the mu-OR agonist [D-Ala(2), N-Me-Phe(4), Gly(2)ol]-enkephalin (DAMGO). Film images were quantified using calibrated [(14)C] standards. Analysis was performed in steroid-responsive hypothalamic regions such as the medial preoptic area (mPOA) and the ventromedial nucleus of the hypothalamus, as well as in non-hypothalamic brain regions. Treatment with estrogen, alone or with progesterone, significantly increased DAMGO-stimulated [(35)S]-GTPgammaS binding in the mPOA when compared to control OVX animals. In addition, estrogen increased mu-OR coupling in the caudate putamen. Steroid treatment had no effect on either basal or DAMGO-stimulated binding in the other brain regions examined. These findings suggest that estrogen modulates mu-OR function in a brain region-specific fashion. This could have important implications in terms of how these hormones synchronize reproductive behavior and gonadotropin release.
journal_name
Neuroendocrinologyjournal_title
Neuroendocrinologyauthors
Acosta-Martinez M,Etgen AMdoi
10.1159/000065953subject
Has Abstractpub_date
2002-10-01 00:00:00pages
235-42issue
4eissn
0028-3835issn
1423-0194pii
65953journal_volume
76pub_type
杂志文章abstract::Changes in antidiuretic substance (i.e., vasopressin) release from the cut pituitary stalk and the intact pituitary gland were observed during electrical stimulation of amygdala medial nucleus. Electrical pulses of 4 or 36 cycles per second (cps) were used and in both cases the same energy (about 2.160 nanowatt second...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000122349
更新日期:1975-01-01 00:00:00
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journal_title:Neuroendocrinology
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journal_title:Neuroendocrinology
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doi:10.1159/000227806
更新日期:2009-01-01 00:00:00
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journal_title:Neuroendocrinology
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doi:10.1159/000125917
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doi:10.1159/000125153
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pub_type: 杂志文章,评审
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更新日期:2007-01-01 00:00:00
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journal_title:Neuroendocrinology
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doi:10.1159/000054456
更新日期:1999-07-01 00:00:00
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doi:10.1159/000123237
更新日期:1981-01-01 00:00:00
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更新日期:2001-04-01 00:00:00
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journal_title:Neuroendocrinology
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doi:10.1159/000126457
更新日期:1993-05-01 00:00:00
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journal_title:Neuroendocrinology
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doi:10.1159/000126159
更新日期:1992-04-01 00:00:00
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journal_title:Neuroendocrinology
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doi:10.1159/000122949
更新日期:1979-01-01 00:00:00
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更新日期:1998-09-01 00:00:00
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journal_title:Neuroendocrinology
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doi:10.1159/000126555
更新日期:1993-09-01 00:00:00
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journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000123300
更新日期:1982-03-01 00:00:00
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journal_title:Neuroendocrinology
pub_type: 杂志文章
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更新日期:2020-04-08 00:00:00
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pub_type: 杂志文章
doi:10.1159/000123360
更新日期:1982-01-01 00:00:00
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更新日期:1988-06-01 00:00:00
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更新日期:2003-03-01 00:00:00
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更新日期:1991-08-01 00:00:00
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更新日期:2020-01-01 00:00:00
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journal_title:Neuroendocrinology
pub_type: 杂志文章
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更新日期:1984-03-01 00:00:00
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更新日期:1988-10-01 00:00:00
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journal_title:Neuroendocrinology
pub_type: 杂志文章
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更新日期:1985-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:2005-01-01 00:00:00
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journal_title:Neuroendocrinology
pub_type: 杂志文章
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更新日期:2002-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:1991-07-01 00:00:00
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journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000126131
更新日期:1992-03-01 00:00:00
abstract::To clarify the role and site of action of gamma-aminobutyric acid (GABA) in ovine pineal glands, we have investigated the effects of aminooxyacetic acid (AOAA), an inhibitor of GABA transaminase, on endogenous GABA content and beta-adrenoceptor mediated pineal function in Merino sheep. A significant elevation of endog...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000123892
更新日期:1984-03-01 00:00:00