Abstract:
AIM:To extensively explore microRNA expression profiles in lung carcinoids in correlation with clinical and pathological features. METHODS:A PCR-based array was employed in the screening phase to analyze 752 microRNAs in a discovery set of 12 lung carcinoids, including 6 typical (3 with lymph node metastasis) and 6 atypical (3 with lymph node metastasis). The results were validated by means of real-time PCR in 37 carcinoids, including 22 typical (4 with lymph node metastasis) and 15 atypical (7 with lymph node metastasis), and 19 high-grade neuroendocrine carcinomas. RESULTS:Unsupervised cluster analysis segregated the pilot cases into 3 distinct groups. Twenty-four microRNAs were differentially regulated in atypical versus typical carcinoids, and 29 in metastatic versus nonmetastatic cases. Eleven microRNAs were selected for validation. All but 1 were significantly different among lung neuroendocrine tumor histotypes. Moreover, 5 (miR-129-5p, miR-409-3p, miR-409-5p, miR-185 and miR-497) were significantly upregulated in typical compared to atypical carcinoids. MiR-409-3p, miR-409-5p and miR-431-5p were also significantly downregulated in carcinoids metastatic to the lymph nodes. Predictive in silico analysis of specific target genes showed that these 3 latter microRNAs linked to metastatic potential are implicated in several cellular functions and highlighted several novel genes which may be worth exploring. CONCLUSIONS:Our findings demonstrate that lung carcinoids have distinct microRNA expression profiles as compared to high-grade neuroendocrine carcinomas and that specific microRNAs might have potential implications as diagnostic tools or clinical biomarkers.
journal_name
Neuroendocrinologyjournal_title
Neuroendocrinologyauthors
Rapa I,Votta A,Felice B,Righi L,Giorcelli J,Scarpa A,Speel EJ,Scagliotti GV,Papotti M,Volante Mdoi
10.1159/000381454subject
Has Abstractpub_date
2015-01-01 00:00:00pages
246-55issue
3eissn
0028-3835issn
1423-0194pii
000381454journal_volume
101pub_type
杂志文章abstract::Lactotrophs, gonadotrophs, thyrotrophs and somatotrophs of the rat anterior pituitary (AP) express 68-kDa neurofilaments (NF68) and other neuronal markers. NF68 expression in the AP appears to be estrogen-dependent, but its significance is unknown. The aims of this work were: (1) to establish the expression pattern of...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000095548
更新日期:2006-01-01 00:00:00
abstract::Using specific antibodies to corticotrophin-releasing factor (CRF) and arginine vasopressin (AVP) we have immunocytochemically located cells and fibres containing these peptides in sheep fetuses. CRF neurones were first detected, in low frequency, in the paraventricular nuclei of fetuses of 90 days of gestation (term ...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000124860
更新日期:1987-11-01 00:00:00
abstract::We measured the concentration of oxytocin and vasopressin in microdissected areas of the hypothalamus and posterior pituitary in weanling and adult female rats. Oxytocin concentration was significantly less in adult rats as compared to weanling rats in the supraoptic nucleus, median eminence, and retrochiasmatic area ...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000123067
更新日期:1980-08-01 00:00:00
abstract::Previous studies indicate that 7 days after testicular administration of naloxone, serum testosterone (T) concentration and basal T secretion in vitro decrease significantly. In neonatal rats, short-term (2 h) intratesticular treatment with 0.3 micrograms (D-Met2-Pro5)-enkephalinamide suppresses steroidogenesis. In th...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000126242
更新日期:1992-09-01 00:00:00
abstract::Pregnancy and parturition are associated with opioid-mediated elevations in maternal pain thresholds. This analgesia is subserved by a spinal cord dynorphin/kappa-opiate receptor system. During gestation, elevated pain thresholds are paralleled by a significant increase in the content of dynorphin (1-17 and 1-8) in th...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000126555
更新日期:1993-09-01 00:00:00
abstract::The ability of the flutamide metabolite Sch 16423 to block androgen receptor binding and inhibit masculine copulatory behavior in male rats was examined. Restoration of copulatory behavior was prevented in Sch 16423-treated rats given two 10-mm testosterone (T)-filled Silastic capsules plus 15 mg Sch 16423 daily for 1...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000124510
更新日期:1986-01-01 00:00:00
abstract:BACKGROUND:The reviewing and assessment of epidemiological characteristics of neuroendocrine tumours (NETs) remains a challenge. Despite the fact that it is an uncommon family of neoplasms, several worldwide series have revealed an increasing incidence of this rare condition. However, the data are difficult to compare ...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000441897
更新日期:2017-01-01 00:00:00
abstract::Dehydration is a classic homeostatic stressor in rats that leads to a series of endocrine responses including stimulation of the hypothalamo-pituitary-adrenal (HPA) axis. During the last decade, it has been well established that perinatal food restriction is associated with the onset of diseases in adults. Our previou...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000078098
更新日期:2004-01-01 00:00:00
abstract::Estrogens are crucial determinants in the regulation of anterior pituitary function and maintenance of tissue homeostasis. Estrogen actions in this gland are exerted through both classical and non-classical mechanisms of action. This review summarizes the expression of classical α- and β-estrogen receptors and variant...
journal_title:Neuroendocrinology
pub_type: 杂志文章,评审
doi:10.1159/000321683
更新日期:2010-01-01 00:00:00
abstract::The effect of stress- or lipopolysaccharide (LPS) endotoxin-induced release of ACTH, beta-endorphin (beta-END) and prolactin (PRL) was investigated in two groups of conscious male rats: (1) Rats pretreated with different H3 receptor agonists, which inhibit neuronal histamine (HA) synthesis and release, and (2) rats wi...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000054402
更新日期:1999-01-01 00:00:00
abstract::A consistent group of gonadotropin-releasing hormone (GnRH) cell bodies occurs in the ventral hypothalamic tract at the infundibular lip (IL), just below the arcuate nucleus (ARC), at the site of the so-called GnRH 'pulse generator'. Immunocytochemical studies were performed to examine contacts between these GnRH neur...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000124914
更新日期:1988-03-01 00:00:00
abstract:BACKGROUND:The outcomes of bariatric surgery are very irregular and mostly unpredictable. The search for variables of predictive value is encouraged to help preventing therapeutic failures. OBJECTIVE:We aimed to confirm the hypothesis that preexisting eating behaviors could predict neuroendocrine and metabolic outcome...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000500687
更新日期:2020-01-01 00:00:00
abstract::Glass microknife lesions and immunocytochemistry were used to evaluate luteinizing hormone-releasing hormone (LH-RH)- and somatostatin (SS)-immunoreactive pathways from the preoptic region to the rat median eminence. Cuts were so placed that axons of more caudally located neurons in the periventricular hypothalamic ar...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000123886
更新日期:1984-03-01 00:00:00
abstract::Over the last 10 years, kisspeptins--peptide products of varying lengths encoded by the KISS1 gene--have been found to be key regulators of normal reproductive function throughout life in animals and humans. By activating the kisspeptin receptor [previously known as orphan G protein-coupled receptor 54 (GPR54)], they ...
journal_title:Neuroendocrinology
pub_type: 杂志文章,评审
doi:10.1159/000439133
更新日期:2015-01-01 00:00:00
abstract::Copulatory behavior in the ovariectomized rat, i.e. the lordosis response (LR) on being mounted by a male, can be induced by administration of either estrogen alone or estrogen followed by progesterone. LR has been shown to be inhibited by lysergic acid diethylamide (LSD) in certain doses (greater than or equal to 50 ...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000123499
更新日期:1983-06-01 00:00:00
abstract::As part of an assessment of endocrine influences on the actions of morphine, we have studied the effects of intracerebroventricular administration of somatostatin and thyrotropin releasing hormone (TRH) on the antinociceptive, temperature and cataleptic responses to subcutaneous morphine in both intact and hypophysect...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000123586
更新日期:1983-12-01 00:00:00
abstract:BACKGROUND:The amygdala receives afferent sensory input and processes information related to hydromineral balance. Angiotensin acts on and through the amygdala to stimulate thirst and sodium appetite. In addition, different angiotensins seem to play a role in cognition and learning mechanisms by acting on and through t...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000328128
更新日期:2011-01-01 00:00:00
abstract::The distribution of gamma-aminobutyric acid (GABA) nerve fibers and cell bodies in the rat hypothalamus and pituitary gland was immunohistochemically examined using antibodies against the GABA-synthesizing enzyme glutamate decarboxylase (GAD). The dense network of GAD-positive nerve fibers was observed to be essential...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000123288
更新日期:1982-02-01 00:00:00
abstract::In previous studies we demonstrated that insulin-like growth factor I (IGF-I) induces pituitary vasoactive intestinal peptide (VIP) gene expression and secretion, and that IGF-I-induced prolactin (PRL) release is mediated by VIP. In this study, we investigate the mitotropic action of IGF-I and VIP on pituitary lactotr...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000070900
更新日期:2003-05-01 00:00:00
abstract::The exact physiological basis of acute growth hormone (GH) suppression by oral glucose is not fully understood. Glucose-mediated increase in hypothalamic somatostatin seems to be the most plausible explanation. Attempts to better understand its underlying mechanisms are compromised by species disparities in the respon...
journal_title:Neuroendocrinology
pub_type: 杂志文章,评审
doi:10.1159/000497214
更新日期:2019-01-01 00:00:00
abstract::Introduction Autophagic cell death in cancer cells can be mediated by inhibition of deacetylases. Although extensive studies have focused on the autophagic process in cancer, little is known about the role of autophagy in degrading cytosolic and nuclear components of pancreatic neuroendocrine neoplastic (pNEN) cells l...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000512567
更新日期:2020-10-27 00:00:00
abstract::It is not known where melatonin acts to influence the neuroendocrine axis of seasonally breeding mammals. However, since the pars tuberalis (PT) contains the highest density of melatonin receptors, this adenohypophyseal subdivision is a potential target. Gonadotropes are the only immunocytochemically detectable adenoh...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000127247
更新日期:1997-10-01 00:00:00
abstract::The storage form of prolactin (PRL) was converted into the releasable form in the lactating rat pituitary gland within 10 min of suckling by 6 pups following 4-5 h of nonsuckling on postpartum day 13-14. The characteristics of the PRL discharge from the releasable pool into the circulation was then studied using a sti...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000123957
更新日期:1984-07-01 00:00:00
abstract::Leptin, the product of the obese gene, is a protein that is secreted primarily from adipocytes. Leptin can influence the function of the pituitary gland through its action on the hypothalamus, but it can also directly act at the level of the pituitary gland. The ability of leptin to induce an increase in intracellular...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000081843
更新日期:2004-01-01 00:00:00
abstract::The mu-opioid receptor (OR) is involved in several aspects of female reproductive neuroendocrinology, such as the control of gonadotropin release and the display of lordosis behavior. Even though the neuroendocrine events modulated by mu-ORs are steroid hormone-dependent, few studies have shown how steroid hormones su...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000065953
更新日期:2002-10-01 00:00:00
abstract::Changes in antidiuretic substance (i.e., vasopressin) release from the cut pituitary stalk and the intact pituitary gland were observed during electrical stimulation of amygdala medial nucleus. Electrical pulses of 4 or 36 cycles per second (cps) were used and in both cases the same energy (about 2.160 nanowatt second...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000122349
更新日期:1975-01-01 00:00:00
abstract::The hypothalamic GnRH pulse generator activity, which was assessed by the characteristic increases in the multiple unit activity (MUA) specifically associated with the pulsatile LH secretion (MUA volleys), was monitored throughout the estrous cycle of the female goat. Blood samples were collected daily for analysis of...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000127051
更新日期:1995-12-01 00:00:00
abstract::Oxytocin receptors are regulated during parturition and lactation. Gonadal steroids are thought to be key players in this regulation. It is not well documented how oxytocin receptor gene expression is regulated in the CNS. In this study we analyzed potential estrogen effects on the oxytocin receptor mRNA levels in som...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000127160
更新日期:1997-01-01 00:00:00
abstract::These studies investigated the effect of aging on thyroid hormone regulation of beta-endorphin in rat corpus striatum and hypothalamus. In both brain areas, basal levels of beta-endorphin declined with age. In addition, age modified the response of beta-endorphin to thyroid hormone status. Hypothyroid rats aged 6 mont...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000123141
更新日期:1981-02-01 00:00:00
abstract::Rats bearing transplanted prolactin-secreting tumors are known to have very high prolactin levels in the serum, reduced hypothalamic opiate concentrations, increasing hypothalamic dopamine synthesis and increased hypophyseal portal blood dopamine levels. Further, it has been shown that incubation in 3H-leucine of ante...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000123211
更新日期:1981-08-01 00:00:00