Abstract：
:Catecholamine uptake into PC12 cells, in the presence and absence of ATP, was measured in a bicarbonate-buffered Krebs Ringer. ATP enhanced significantly the uptake in a dose-dependent manner with maximal uptake at 1-3 mM. ATP increased the Vmax of uptake by 3-5 fold for both dopamine and norepinephrine, without a significant change in the Km. ATP-stimulated amine uptake was temperature- and Na(+)-dependent and robust in bicarbonate, but not in HEPES buffer. The ability to enhance uptake was not observed with metabolites of ATP. GTP and UTP were equally effective to ATP in enhancing CA uptake. This uptake was less sensitive to uptake inhibitors in bicarbonate buffered media than in phosphate-buffered media, and more so in the presence of ATP. It is suggested that ATP is an allosteric modulator of the transporter and that a stable ATP analog, with ATP-like enhancement of dopamine uptake, may be an effective cocaine antagonist.
journal_name
Life Scijournal_title
Life sciencesauthors
Eshleman A,Dunigan C,Shamoo A,Eldefrawi Mdoi
10.1016/0024-3205(95)00128-ssubject
Has Abstractpub_date
1995-01-01 00:00:00pages
1613-21issue
19eissn
0024-3205issn
1879-0631pii
002432059500128Sjournal_volume
56pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract：AIMS:Cisplatin is an anticancer agent marred by nephrotoxicity. Limiting this adverse effect may allow the use of higher doses to improve its efficacy. The Wnt/β-catenin signaling pathway plays a critical role in nephrogenesis and repair of renal diseases. BIO, a small molecule agonist of this pathway, exerted a protec...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2020.118672
更新日期：2020-12-15 00:00:00
abstract：:SR 142801 is the first potent and selective non-peptide antagonist of the tachykinin NK3 receptor. It inhibited [MePhe7]NKB binding to its receptor from various species, including humans. SR 142801 was a competitive antagonist of [MePhe7]NKB-mediated contractions of guinea-pig ileum and inhibited the acetylcholine rel...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(94)00413-m
更新日期：1995-01-01 00:00:00
abstract：:Classical studies have demonstrated that the visual centers in primates consist of cortical areas V1, V2 and V4 and their branches. However, nothing is known about how these visual areas change in postnatal development. In the present studies, therefore, pigs aged 2, 4, and 6 months old, were stimulated visually with ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2005.06.030
更新日期：2006-02-09 00:00:00
abstract：:Experiments were done to examine the analgesic effect of thiorphan alone or in combination with stress in mice. Analgesia was assessed by measuring jump latencies from a 55 degrees C hot plate. Thiorphan exhibited weak analgesic properties evidenced by significant increases in jump latencies only after 300 mg/kg i.p. ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(82)90338-1
更新日期：1982-09-20 00:00:00
abstract：:Immunization with plasmid DNA encoding antigenic proteins elicits both antibody and cell-mediated immune responses. This method of producing the protein antigens of interest directly in host cells can provide appropriate tertiary structure for the induction of conformationally specific antibodies, and also facilitates...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/s0024-3205(96)00502-4
更新日期：1997-01-01 00:00:00
abstract：:Time-course studies revealed the increased susceptibility of the gastric mucosa to noxious injury in portal hypertension correlates with the level of elevated portal venous pressure and hyperglucagonemia. Whether acute elevation of portal venous pressure by exogenous glucagon aggravates such injury is not known. We te...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(03)00413-2
更新日期：2003-07-18 00:00:00
abstract：:We have developed and utilized a photoaffinity probe to identify the adenosine transporter in cardiac sarcolemmal (SL) vesicles. The probe is an azidosalicylate derivative of adenosine made by reacting adenosine with N-hydroxysuccinimidyl-4-azidosalicylic acid (NHS-ASA). Following synthesis and radiolabeling of the pr...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(95)00268-b
更新日期：1995-01-01 00:00:00
abstract：:Silica (SiO2) nanoparticles (NPs) were synthesized by laser ablation method and were characterized by TEM and DLS techniques. Afterwards, their inhibition activity against carbonic anhydrase (CA) isoforms (CA I and CA II) was explored by experimental and theoretical analysis. Also, the protective effect of SiO2 NPs ag...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2021.119032
更新日期：2021-01-12 00:00:00
abstract：:Regulation of glucose-induced oscillations in intracellular Ca2+ concentration ([Ca2+]i) was investigated by using a novel technique, electroporation from an electrolyte-filled capillary, to deliver energy metabolites to the intracellular compartment of mouse islets. Intracellular application of ATP resulted in a nife...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2005.05.053
更新日期：2005-10-21 00:00:00
abstract：:A recent neuropathological study has reported decreased levels of dynorphin A immunoreactivity in striato-pallidal fibers in the brain of a patient with severe Gilles de la Tourette's syndrome (TS). This observation, taken with the neuroanatomic distribution of dynorphin and its broad range of motor and behavioral eff...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(88)90575-9
更新日期：1988-01-01 00:00:00
abstract：:The melanocortins are a family of bioactive peptides derived from proopiomelanocortin. Those peptides, included among hormones and comprising ACTH, alpha-MSH, beta-MSH and gamma-MSH, are best known mainly for their physiological effects, such as the control of skin pigmentation by alpha-MSH, and ACTH effects on pigmen...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/s0024-3205(03)00349-7
更新日期：2003-07-04 00:00:00
abstract：:To investigate the changes in the FSH receptor and the activin receptor during sexual maturation in rat testes, we examined the mRNA levels of the receptors by Northern blot analysis. With a full length rat activin receptor cRNA probe in this study, Northern blot analysis revealed two activin receptor mRNAs (6Kb and 3...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(93)90575-n
更新日期：1993-01-01 00:00:00
abstract：:The effects of 1,3-dipropyl-8(p-sulfophenyl)xanthine (DPSPX) and enprofylline on the respiratory responses to bilateral occlusions of the common carotid arteries (CCO) were studied in rats anesthetized with sodium pentobarbitone and breathing spontaneously. CCO during periods of 5, 10 and 15 s caused increases in tida...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(89)90208-7
更新日期：1989-01-01 00:00:00
abstract：:Oxytocin (OT) and arginine-8-vasopressin (AVP) were measured by radioimmunoassay in micropunched hypothalamic neurosecretory nuclei of estrous cycling female Sprague-Dawley rats. In the paraventricular nucleus (PVN): the concentration (pg/microgram protein) of OT was significantly higher in rats in diestrus than durin...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(86)90638-7
更新日期：1986-06-23 00:00:00
abstract：:Estrogen has diverse effects on the vasculature, such as vasodilation, endothelial growth and inhibition of vascular smooth muscle cell (VSMC) proliferation and migration. However, little is known about the genes that are regulated by estrogen in the vascular wall. Wistar rats were ovariectomized or sham-operated (Sha...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2004.03.004
更新日期：2004-07-23 00:00:00
abstract：AIMS:Cyclooxygenase-2 (COX-2)-controlled production of prostaglandin E(2) (PGE(2)) has been implicated in cell growth and metastasis in many cancers. Recent studies have found that COX-2 is co-expressed with survivin in many cancers. Survivin is a member of the inhibitor-of-apoptosis protein family. Some COX-2 inhibito...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2009.12.009
更新日期：2010-01-30 00:00:00
abstract：:Vascular smooth muscle cell (VSMC) accumulation and endothelial cell dysfunction are associated with pathogenesis of atherosclerosis. Long noncoding RNA taurine up-regulated gene 1 (TUG1) has been reported to play an important role in cardiovascular diseases, including atherosclerosis. However, the regulatory mechanis...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2020.117287
更新日期：2020-02-15 00:00:00
abstract：:Nociceptin/orphanin FQ (=N/OFQ), the endogenous ligand of ORL1 receptor (=NOP), has been reported to induce, in rodents, after intracerebroventricular (i.c.v.) administration, anti-stress and anxiolytic effects. We have observed that the handling of mice followed by an i.c.v. injection of saline, induced a marked incr...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(02)02218-x
更新日期：2002-11-29 00:00:00
abstract：:Decreased heart rate variability (HRV) and heart rate turbulence (HRT) are independent predictors of mortality after acute myocardial infarction (AMI). There are no previous studies on the relationship between warm-up phenomenon and cardiac autonomic control in stable coronary artery disease (CAD). We investigated the...
journal_title：Life sciences
pub_type： 临床试验,杂志文章
doi：10.1016/j.lfs.2004.06.033
更新日期：2005-03-25 00:00:00
abstract：:Fluorescence spectroscopy was used to examine the biophysical characteristics of human platelet membranes as a function of subject age. The structural order of membrane lipid domains was determined with the use of 1,6-diphenyl-1,3,5-hexatriene (DPH), a fluorescent probe that preferentially localizes in the hydrocarbon...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(85)90079-7
更新日期：1985-10-14 00:00:00
abstract：:According to our previously proposed auto-addiction hypothesis of chronic anorexia nervosa, patients become addicted to an initial period of dieting through endogenous opioid mediated mechanisms. Morphine causes hyperactivity and anorexia in the mouse, symptoms of anorexia nervosa but responses opposite to those of mo...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(87)90457-7
更新日期：1987-08-10 00:00:00
abstract：:Alzheimer's disease (AD) is the most common form of dementia worldwide. β-amyloid peptide (Aβ) is currently assumed to be the main cause of synaptic dysfunction and cognitive impairments in AD, but the molecular signaling pathways underlying its neurotoxic consequences have not yet been completely explored. Additional...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/j.lfs.2020.118020
更新日期：2020-09-15 00:00:00
abstract：:Involvement of cyclic AMP-dependent protein kinase in the desensitization of GABAB receptor expressed in Xenopus oocytes was studied. GABA produced an outward current in the presence of bicuculline, under voltage-clamp conditions. Baclofen mimicked the effect of GABA, thereby indicating that the response to GABA in th...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(95)02235-1
更新日期：1995-11-17 00:00:00
abstract：AIMS:Nicotine is a risk factor for various diseases, including osteoporosis, oral cancer, and periodontal disease. Numerous studies have elucidated the effects of nicotine on cell proliferation and differentiation. The purpose of this study was to determine the effects of nicotine on the proliferation and osteoblast di...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2011.10.019
更新日期：2012-01-16 00:00:00
abstract：:Artemisinin is a promising and a potent antimalarial drug, which meets the dual challenge posed by drug-resistant parasites and rapid progression of malarial illness. This review article focuses on the progress achieved during the last years in the production of artemisinin from Artemisia annua. The structure, biosynt...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/s0024-3205(99)00356-2
更新日期：2000-01-01 00:00:00
abstract：:The effect on thermonociceptive threshold of intrathecally (i.t.) administered angiotensin II (Ang II) was assessed in the rat tail-flick test. Rats were pretreated, 15 min earlier, with i.t. naloxone (opiate antagonist), losartan (Ang II selective antagonist at AT1 receptor) or [Sar1, Leu8] Ang II (non selective Ang ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(97)00410-4
更新日期：1997-01-01 00:00:00
abstract：:The traditional Chinese medicines have been used clinically for a long time in some Asian countries, however, very few studies have been done to demonstrate the working mechanisms of these medicines using recently developed biochemical methodologies. In this study, we examined the anti-inflammatory effect of Huang-Lia...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(99)00557-3
更新日期：2000-01-01 00:00:00
abstract：:Depression is a leading cause of disability worldwide. However, the biological and molecular mechanisms underlying this disease remain unclear. Stress, a disposing factor in the development of depression, leads to hypothalamic-pituitary-adrenal (HPA) axis activation and glucocorticoids release. Glucocorticoids at phys...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/j.lfs.2016.03.022
更新日期：2016-05-01 00:00:00
abstract：:P75(NTR) is a common neurotrophin receptor which binds all neurotrophins with similar affinities and has been shown to be capable of mediating programmed cell death. In this study, we investigated effects of the antipsychotic drugs (APDs) haloperidol, clozapine, quetiapine, and risperidone on p75(NTR) mRNA levels in P...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2006.01.039
更新日期：2006-07-04 00:00:00
abstract：:Linear sucrose density gradient centrifugation of a crude synaptosomal-mitochondrial preparation of rat striatum was performed at 82,500g for 7.5, 15 and 30 min and 1, 4 and 20 h. After centrifugation various marker enzyme activities were measured throughout the gradients, viz. tyrosine hydroxylase (TH) and DOPA decar...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(83)90248-5
更新日期：1983-08-15 00:00:00
